FastQCFastQC Report
Fri 27 May 2016
SRR2140070_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140070_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20047205
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA281950.051.5721551
GTATCAA546600.040.0239871
ATCAACG667100.032.179723
TCAACGC668950.032.0652854
CAACGCA678550.031.61795
TATCAAC694300.031.21542
AACGCAG700600.030.7321136
ACGCAGA803850.026.7290787
CGCAGAG819400.026.1988418
GTACATG711200.022.4618191
GCAGAGT954850.022.4378579
TACATGG710400.021.9479962
ACATGGG712500.021.3383183
GAGTACT540350.019.98636812-13
AGAGTAC857450.019.41477610-11
CAGAGTA922600.019.10206810-11
CATGGGG486900.018.9116544
GTACTTT588600.018.39081614-15
AGTACTT583900.017.87637512-13
GTACTAG52100.017.5607511
Produced by FastQC (version 0.11.5)