FastQCFastQC Report
Fri 27 May 2016
SRR2140069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22576622
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA328550.051.8710861
GTATCAA589700.038.0376321
TCAACGC716300.030.6703954
ATCAACG717550.030.6169643
CAACGCA730100.030.0671395
TATCAAC745350.029.7656962
AACGCAG753650.029.1903176
ACGCAGA870150.025.2099137
CGCAGAG887850.024.7201548
GTACATG790050.022.2455351
TACATGG789050.021.8761772
ACATGGG795950.021.117943
GCAGAGT1035400.021.0936749
GAGTACT588850.019.72316212-13
CATGGGG552300.019.2314474
CAGAGTA1005200.019.0050710-11
GTACTTT625800.018.53682714-15
AGAGTAC930450.018.5027110-11
AGTACTT630250.016.98595612-13
GTGGTAT147350.016.9433821
Produced by FastQC (version 0.11.5)