##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140069_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22576622
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56273737497133	33.0	31.0	34.0	28.0	34.0
2	32.050810302799064	33.0	31.0	34.0	30.0	34.0
3	32.08064240079849	33.0	31.0	34.0	30.0	34.0
4	35.39510051592306	37.0	35.0	37.0	33.0	37.0
5	35.383876471865456	37.0	35.0	37.0	33.0	37.0
6	35.452667631145175	37.0	35.0	37.0	33.0	37.0
7	35.53752913079734	37.0	35.0	37.0	33.0	37.0
8	35.71166448195837	37.0	35.0	37.0	35.0	37.0
9	37.660108540595665	39.0	38.0	39.0	35.0	39.0
10-11	37.79134557862554	39.0	38.0	39.0	35.0	39.0
12-13	37.81773686072256	39.0	38.0	39.0	35.0	39.0
14-15	39.23046350778252	41.0	39.0	41.0	36.0	41.0
16-17	39.25985357774073	41.0	39.0	41.0	36.0	41.0
18-19	39.30117235873462	41.0	39.0	41.0	36.0	41.0
20-21	39.317081126285316	41.0	39.0	41.0	36.0	41.0
22-23	39.27874463741472	41.0	39.0	41.0	36.0	41.0
24-25	39.259628645954876	41.0	39.0	41.0	36.0	41.0
26-27	39.18135943261087	41.0	39.0	41.0	36.0	41.0
28-29	39.11925445712879	41.0	39.0	41.0	35.5	41.0
30-31	39.038176952859075	41.0	39.0	41.0	35.0	41.0
32-33	38.9595579323057	41.0	39.0	41.0	35.0	41.0
34-35	38.888889362826575	40.0	38.0	41.0	35.0	41.0
36-37	38.83699440142699	40.0	38.0	41.0	35.0	41.0
38-39	38.79500492694992	40.0	38.0	41.0	35.0	41.0
40-41	38.74820067276213	40.0	38.0	41.0	35.0	41.0
42-43	38.68664080663825	40.0	38.0	41.0	35.0	41.0
44-45	38.61205280946942	40.0	38.0	41.0	34.5	41.0
46-47	38.54028270197535	40.0	38.0	41.0	34.0	41.0
48-49	38.46250326538694	40.0	38.0	41.0	34.0	41.0
50-51	38.34901739399156	40.0	38.0	41.0	34.0	41.0
52-53	38.241140741279615	40.0	37.5	41.0	34.0	41.0
54-55	38.09899841193083	40.0	37.0	41.0	33.5	41.0
56-57	37.93734385167054	40.0	37.0	41.0	33.0	41.0
58-59	37.739981463383415	39.0	36.0	41.0	33.0	41.0
60-61	37.535401711952005	39.0	36.0	41.0	33.0	41.0
62-63	37.30130537908971	39.0	35.0	41.0	33.0	41.0
64-65	37.29149154424783	39.0	35.0	41.0	33.0	41.0
66-67	37.07013144138286	38.0	35.0	40.5	33.0	41.0
68-69	36.783715690084065	37.0	35.0	40.0	33.0	41.0
70-71	36.47751993810007	37.0	35.0	39.5	32.0	41.0
72-73	36.15629765742261	36.5	35.0	39.0	32.0	41.0
74-75	35.82024642361337	36.0	35.0	39.0	32.0	40.5
76-77	35.490805326889614	35.5	35.0	37.5	32.0	39.5
78-79	35.19569270172765	35.0	35.0	37.0	32.0	39.0
80-81	34.91130588863612	35.0	35.0	37.0	31.0	39.0
82-83	34.66242779662024	35.0	34.0	36.0	31.0	38.0
84-85	34.43427748046176	35.0	34.0	36.0	31.0	37.0
86-87	34.23945669595004	35.0	34.0	35.5	31.0	37.0
88-89	34.09747325640504	35.0	34.0	35.0	31.0	36.5
90-91	33.98280024813121	35.0	34.0	35.0	31.0	36.0
92-93	33.905631614640356	35.0	34.0	35.0	31.0	36.0
94-95	33.85345229881881	35.0	34.0	35.0	31.0	36.0
96-97	33.84859592197397	35.0	34.0	35.0	31.0	35.5
98-99	33.88511395826056	35.0	34.0	35.0	31.0	35.0
100	33.31264798637173	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	8.0
17	257.0
18	2237.0
19	8220.0
20	16578.0
21	26388.0
22	37373.0
23	50924.0
24	67743.0
25	89449.0
26	116213.0
27	148293.0
28	184659.0
29	228238.0
30	280537.0
31	350020.0
32	444523.0
33	588046.0
34	845692.0
35	1290164.0
36	2240599.0
37	4752757.0
38	6983233.0
39	3639428.0
40	185043.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.36686861302812	18.834496143842955	10.142482785954426	27.656152457174503
2	14.489015519735387	21.712316008931197	38.75483752794321	25.043830943390212
3	18.100559950908508	26.71234872958408	28.573003525505275	26.614087794002135
4	12.21654678078537	18.644823941451968	37.70616304689513	31.432466230867533
5	13.2993589563576	38.647973997172826	34.27815729031562	13.774509756153956
6	29.519079648184785	38.62897337349652	18.436171286763756	13.415775691554934
7	26.60095868680904	33.45850186691875	22.27908054306981	17.6614589032024
8	25.199890399901275	36.54256602249885	20.35007717274976	17.907466404850116
9	26.592512378512605	16.693081896840013	20.2978815874226	36.41652413722478
10-11	24.51906666993849	26.83726777194569	28.22043528035328	20.423230277762546
12-13	25.53425175830113	24.472485299173634	28.447754495778867	21.54550844674637
14-15	22.67164901817464	25.408426911696537	26.327977675313875	25.591946394814958
16-17	21.98001506957798	28.43817878600114	26.979901899359476	22.601904245061405
18-19	22.046247662737144	27.59358773867942	28.802453263380144	21.55771133520329
20-21	22.991876283097177	26.75555562841641	28.632726421866455	21.61984166661996
22-23	22.666773918117062	26.661956203061926	28.51501389898558	22.156255979835425
24-25	22.369907362187913	26.78923258759464	28.608133352034244	22.2327266981832
26-27	22.30539498891225	26.942794491927707	28.515109755858315	22.236700763301723
28-29	22.291835144579395	26.929396304934993	28.634151999866926	22.144616550618686
30-31	22.59674723410659	26.76001150196833	28.484308378337204	22.15893288558788
32-33	22.31642479512212	27.010389116711668	28.51724588384124	22.15594020432497
34-35	22.457977113827095	26.969587220040943	28.507781155883922	22.064654510248047
36-37	22.4711780479482	26.84310708770813	28.497603719060994	22.188111145282676
38-39	22.331420026414072	27.0149557116888	28.549590961917993	22.104033299979136
40-41	22.568823218705656	26.852842104333114	28.388626349371876	22.189708327589358
42-43	22.446375977459486	26.96588503352528	28.204523335434267	22.383215653580972
44-45	22.534939120364296	27.139691161587248	27.997433908220202	22.327935809828254
46-47	22.689143021039122	27.108411487343957	27.607369833387796	22.59507565822912
48-49	22.611670645812644	27.2397954999363	27.572651538541365	22.5758823157097
50-51	22.589738543540918	27.406847786520995	27.445295497056875	22.558118172881215
52-53	22.776574480851032	27.304197285332695	27.32590102510642	22.59332720870985
54-55	22.736718200589216	27.18328430366359	27.392353569910348	22.687643925836838
56-57	22.68882387723737	27.35522490288538	27.380582639354394	22.575368580522856
58-59	22.686110256189426	27.362319505240034	27.394582581124123	22.556987657446417
60-61	22.725446737606195	27.35778296352529	27.342819881657842	22.57395041721067
62-63	22.696018518541294	27.447290610586965	27.331881786140954	22.524809084730787
64-65	22.702154809695607	27.33996756136538	27.301704219712423	22.65617340922659
66-67	22.72726206615098	27.411852870023424	27.17279195897005	22.688093104855543
68-69	22.6694732037294	27.36732726455653	27.295320608410606	22.66787892330347
70-71	22.73346567810274	27.110631892821168	27.291150895221065	22.86475153385502
72-73	22.73083777854355	27.17024973858634	27.281049714875255	22.817862767994853
74-75	22.56810689628241	27.346271989884247	27.309196726145768	22.77642438768758
76-77	22.754646939186834	27.276039843897802	27.184761075428156	22.784552141487207
78-79	22.693446243430422	27.336327816390842	27.16222280064961	22.80800313952912
80-81	22.73387509576184	27.384488940970346	27.135247969605995	22.746387993661823
82-83	22.780387228928724	27.296410260949884	27.2377559364173	22.685446573704084
84-85	22.832262823084598	27.294555248577385	27.17804626581338	22.69513566252464
86-87	22.611515761564778	27.38862542924722	27.300821035432595	22.69903777375541
88-89	22.705159871151555	27.33536711289396	27.188144569877664	22.77132844607682
90-91	22.73380342152437	27.339705663740656	27.23070393787999	22.695786976854983
92-93	22.755902342480667	27.323971750603445	27.23604615595847	22.684079750957423
94-95	22.58525423401327	27.40106712705393	27.281306579953835	22.73237205897897
96-97	22.701832047527294	27.481853082859793	27.33004216760869	22.486272702004232
98-99	23.72997169568907	29.482436890150428	27.46845267614624	19.319138738014267
100	22.810446727144033	30.36916309893385	21.28136666051915	25.539023513402974
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10422.0
1	9221.0
2	5821.0
3	3495.0
4	3580.0
5	5295.0
6	8601.5
7	14646.0
8	24259.5
9	30856.0
10	30610.5
11	29138.0
12	32487.5
13	42249.5
14	65889.0
15	99444.5
16	127155.0
17	143417.0
18	144800.0
19	132661.5
20	113663.5
21	93333.5
22	79576.5
23	79694.5
24	92781.5
25	117186.5
26	152353.5
27	192991.0
28	231839.5
29	275222.0
30	320845.0
31	363359.5
32	412794.5
33	465864.0
34	518116.0
35	567069.5
36	619752.5
37	670702.0
38	710894.0
39	744878.0
40	779554.5
41	810858.5
42	833803.5
43	861596.5
44	890880.5
45	922518.0
46	946399.5
47	960059.0
48	971594.0
49	970583.0
50	958938.0
51	937801.5
52	903621.5
53	857449.5
54	807986.5
55	754726.0
56	698323.5
57	636138.0
58	570109.5
59	503254.0
60	423878.5
61	342970.0
62	271733.5
63	210418.0
64	157961.5
65	115435.0
66	85003.5
67	63961.5
68	47615.5
69	34277.5
70	24338.0
71	17061.0
72	11434.5
73	7248.5
74	4581.0
75	2809.5
76	1806.0
77	1143.5
78	729.0
79	503.0
80	362.5
81	264.0
82	185.5
83	120.0
84	86.5
85	64.0
86	49.5
87	45.0
88	37.0
89	28.0
90	21.5
91	16.0
92	14.0
93	12.0
94	6.0
95	3.5
96	3.0
97	3.5
98	8.5
99	13.5
100	25.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008048148212784004
3	0.0
4	5.7581687818487634E-5
5	0.0
6	0.01056402503439177
7	0.008823286318032874
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	4.872296661564339E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.431760298495778E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	1.3805221059729015E-4
50-51	0.01404654606114051
52-53	3.5610909786811017E-4
54-55	0.0
56-57	0.008166362617635384
58-59	0.02402791396557613
60-61	0.004692835139480729
62-63	0.0012913224728603985
64-65	0.002060748589712337
66-67	0.001055439913219385
68-69	0.0015992841242157944
70-71	2.7888380559770506E-5
72-73	0.005653940163676456
74-75	0.0018288720789499861
76-77	0.01981343079575172
78-79	9.424233027774393E-5
80-81	3.3547238804698676E-4
82-83	0.004015358934561568
84-85	0.0030672062833173603
86-87	0.0033803195946918068
88-89	0.0
90-91	5.543775801945283E-6
92-93	0.0
94-95	0.0018156401286473377
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	4507.0
20-21	11108.0
22-23	13499.0
24-25	13587.0
26-27	15514.0
28-29	22190.0
30-31	40374.0
32-33	48542.0
34-35	65726.0
36-37	101578.0
38-39	135717.0
40-41	139827.0
42-43	161907.0
44-45	199388.0
46-47	190967.0
48-49	169622.0
50-51	147781.0
52-53	135786.0
54-55	141805.0
56-57	147679.0
58-59	152164.0
60-61	153619.0
62-63	141303.0
64-65	145415.0
66-67	153585.0
68-69	160241.0
70-71	163257.0
72-73	152026.0
74-75	149984.0
76-77	156610.0
78-79	168602.0
80-81	176434.0
82-83	170859.0
84-85	167226.0
86-87	175367.0
88-89	192179.0
90-91	210914.0
92-93	221531.0
94-95	266653.0
96-97	759070.0
98-99	1439357.0
100-101	1.5193122E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.09413645559869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.67157282835332	68.55213208107138
2	8.617053332903389	13.631167843156645
3	2.3752911243061225	5.636148009229227
4	0.9795047886181274	3.098923416394981
5	0.48437876328708845	1.9155759999811555
6	0.2675725869683244	1.2698053623270118
7	0.16907491969304142	0.9360984338594565
8	0.10795947454489314	0.6831169129062808
9	0.07426769143228995	0.5286725028349055
>10	0.24416322146623481	2.9595687817791765
>50	0.006423840005659971	0.35066787705214425
>100	0.0026589212238684514	0.37143215984543115
>500	6.544288326018709E-5	0.035732632073496536
>1k	1.30643143745637E-5	0.030957987488663796
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3288081804266378E-5
2	0.0	3.100552420995488E-5	0.0	4.429360601422126E-6	1.7717442405688503E-5
3	0.0	3.9864245412799135E-5	0.0	4.429360601422126E-6	1.7717442405688503E-5
4	4.429360601422126E-6	6.201104841990976E-5	0.0	4.429360601422126E-6	2.6576163608532756E-5
5	8.858721202844251E-6	6.201104841990976E-5	0.0	4.429360601422126E-6	2.6576163608532756E-5
6	3.5434884811377005E-5	6.201104841990976E-5	0.0	4.429360601422126E-6	4.429360601422126E-5
7	3.5434884811377005E-5	6.201104841990976E-5	0.0	4.429360601422126E-6	4.429360601422126E-5
8	3.5434884811377005E-5	7.086976962275401E-5	0.0	4.429360601422126E-6	5.315232721706551E-5
9	3.5434884811377005E-5	1.904625058611514E-4	0.0	4.429360601422126E-6	6.64404090213319E-5
10-11	3.9864245412799135E-5	1.904625058611514E-4	0.0	8.858721202844251E-6	6.64404090213319E-5
12-13	3.9864245412799135E-5	2.0153590736470673E-4	0.0	1.3288081804266376E-5	6.64404090213319E-5
14-15	5.5367007517776576E-5	3.16699283001682E-4	0.0	2.214680300711063E-5	9.301657262986463E-5
16-17	1.306661377419527E-4	3.2998736480594837E-4	0.0	3.100552420995488E-5	1.0187529383270889E-4
18-19	1.373101786440859E-4	3.52134167813059E-4	0.0	3.100552420995488E-5	1.0630465443413102E-4
20-21	1.4173953924550802E-4	3.764956511208807E-4	0.0	3.100552420995488E-5	1.1073401503555315E-4
22-23	1.4616889984693016E-4	4.362920192400794E-4	0.0	3.764956511208807E-5	1.5281294074906334E-4
24-25	1.4616889984693016E-4	4.872296661564339E-4	0.0	4.872296661564339E-5	1.904625058611514E-4
26-27	1.4838358014764122E-4	5.093764691635445E-4	0.0	7.972849082559826E-5	1.9489186646257355E-4
28-29	1.5059826044835228E-4	5.115911494642555E-4	0.0	2.3032675127395054E-4	1.9489186646257355E-4
30-31	1.5059826044835228E-4	5.603141160798989E-4	0.0	7.928555476545605E-4	1.9932122706399567E-4
32-33	1.5059826044835228E-4	5.6252879638061E-4	0.0	0.0018559020919958708	2.214680300711063E-4
34-35	1.5059826044835228E-4	6.378279266047861E-4	0.0	0.0032622240829473957	2.3475611187537268E-4
36-37	1.550276210497744E-4	7.175564174303844E-4	0.0	0.0053085886808044176	2.391854724767948E-4
38-39	1.550276210497744E-4	7.640647037453167E-4	0.0	0.009051398389006114	2.4361483307821691E-4
40-41	1.550276210497744E-4	8.637253172773145E-4	0.0	0.013128624822615183	2.4804419367963906E-4
42-43	1.550276210497744E-4	9.058042429908248E-4	0.0	0.016926801538334653	2.502588739803501E-4
44-45	1.550276210497744E-4	9.966061353199785E-4	0.0	0.02094644628412523	2.5468823458177223E-4
46-47	1.550276210497744E-4	0.0010696905852434434	0.0	0.02512111865096559	2.657616360853275E-4
48-49	1.550276210497744E-4	0.0010763346261455768	0.0	0.02944638927825429	2.879084390924382E-4
50-51	1.7717442405688503E-4	0.0011826392805797076	0.0	0.033638779087500334	2.879084390924382E-4
52-53	1.8160378465830718E-4	0.001195927362383974	0.0	0.03830732516139926	3.16699283001682E-4
54-55	1.8160378465830718E-4	0.0012247182062932178	0.0	0.04295372443229107	3.632075693166143E-4
56-57	1.860331452597293E-4	0.0012490796896010394	0.0	0.04744952544273452	3.720662905194586E-4
58-59	1.860331452597293E-4	0.0012579384108038836	0.0	0.051752649267016115	3.85354372323725E-4
60-61	1.926771861618625E-4	0.0012845145744124166	0.0	0.05516547161041187	4.0750117533083557E-4
62-63	2.2589739067252842E-4	0.0013044466971188161	0.0	0.058385616767645754	4.252186177365241E-4
64-65	2.2589739067252842E-4	0.00133323754102806	0.0	0.06079297425451868	4.296479783379462E-4
66-67	2.3475611187537268E-4	0.0013465256228323262	0.0	0.06298329307192192	4.385066995407905E-4
68-69	2.3475611187537268E-4	0.00137531646674157	0.0	0.06531756610887138	4.429360601422126E-4
70-71	2.45829513378928E-4	0.0013908192288465476	0.0	0.06691656528598477	4.5179478134505684E-4
72-73	2.9233779969386033E-4	0.0013996779500493918	0.0	0.0682431587861107	4.960883873592781E-4
74-75	2.945524799945714E-4	0.0014218247530565025	0.0	0.069405865943984	4.960883873592781E-4
76-77	3.034112011974156E-4	0.001503767924182812	0.0	0.07037811059599616	4.983030676599892E-4
78-79	3.056258814981267E-4	0.0015502762104977442	0.0	0.07099379171959383	5.005177479607002E-4
80-81	3.056258814981267E-4	0.0015547055710991662	0.0	0.0711864689057557	5.049471085621223E-4
82-83	3.056258814981267E-4	0.0015547055710991662	0.0	0.07144115714033747	5.403819933734994E-4
84-85	3.0784056179883774E-4	0.0015635642923020104	0.0	0.07158732604018439	5.470260342756326E-4
86-87	3.100552420995488E-4	0.0015901404559105431	0.0	0.07164047836740146	5.492407145763436E-4
88	3.100552420995488E-4	0.0016964451103446741	0.0	0.07165376644920574	5.536700751777657E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	32855	0.0	51.871086	1
GTATCAA	58970	0.0	38.037632	1
TCAACGC	71630	0.0	30.670395	4
ATCAACG	71755	0.0	30.616964	3
CAACGCA	73010	0.0	30.067139	5
TATCAAC	74535	0.0	29.765696	2
AACGCAG	75365	0.0	29.190317	6
ACGCAGA	87015	0.0	25.209913	7
CGCAGAG	88785	0.0	24.720154	8
GTACATG	79005	0.0	22.245535	1
TACATGG	78905	0.0	21.876177	2
ACATGGG	79595	0.0	21.11794	3
GCAGAGT	103540	0.0	21.093674	9
GAGTACT	58885	0.0	19.723162	12-13
CATGGGG	55230	0.0	19.231447	4
CAGAGTA	100520	0.0	19.00507	10-11
GTACTTT	62580	0.0	18.536827	14-15
AGAGTAC	93045	0.0	18.50271	10-11
AGTACTT	63025	0.0	16.985956	12-13
GTGGTAT	14735	0.0	16.943382	1
>>END_MODULE