FastQCFastQC Report
Fri 27 May 2016
SRR2140068_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140068_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20003910
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA323350.052.009261
GTATCAA599350.039.1602821
ATCAACG738800.031.1196353
TCAACGC742500.030.9415864
TATCAAC757950.030.6284162
CAACGCA749900.030.607825
AACGCAG774150.029.7537086
ACGCAGA884300.025.9914957
CGCAGAG897450.025.589668
GCAGAGT1036550.022.003459
GTACATG750300.021.692031
TACATGG741150.021.5972882
ACATGGG755750.020.704353
GAGTACT593950.020.47784212-13
CAGAGTA998600.019.50486610-11
GTACTTT636350.019.09615714-15
AGAGTAC935850.018.9628810-11
CATGGGG535300.018.8339864
CTTATAC118700.018.1095051
AGTACTT631600.018.0824812-13
Produced by FastQC (version 0.11.5)