##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140068_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20003910
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.559257215214426	33.0	31.0	34.0	28.0	34.0
2	32.04464957100887	33.0	31.0	34.0	30.0	34.0
3	32.07386126012364	33.0	31.0	34.0	30.0	34.0
4	35.3934087385916	37.0	35.0	37.0	33.0	37.0
5	35.38902754511493	37.0	35.0	37.0	33.0	37.0
6	35.46070763165801	37.0	35.0	37.0	33.0	37.0
7	35.5434161621403	37.0	35.0	37.0	33.0	37.0
8	35.71877267994107	37.0	35.0	37.0	35.0	37.0
9	37.665232397066376	39.0	38.0	39.0	35.0	39.0
10-11	37.79376956804945	39.0	38.0	39.0	35.0	39.0
12-13	37.81656438666241	39.0	38.0	39.0	35.0	39.0
14-15	39.22354769642535	41.0	39.0	41.0	36.0	41.0
16-17	39.254109346622734	41.0	39.0	41.0	36.0	41.0
18-19	39.29626880444873	41.0	39.0	41.0	36.0	41.0
20-21	39.31340256503853	41.0	39.0	41.0	36.0	41.0
22-23	39.27370591908192	41.0	39.0	41.0	36.0	41.0
24-25	39.25141915745343	41.0	39.0	41.0	36.0	41.0
26-27	39.169061078328035	41.0	39.0	41.0	36.0	41.0
28-29	39.105824178771975	41.0	39.0	41.0	35.0	41.0
30-31	39.018779243777765	41.0	39.0	41.0	35.0	41.0
32-33	38.93268037280574	40.5	38.5	41.0	35.0	41.0
34-35	38.859223191466796	40.0	38.0	41.0	35.0	41.0
36-37	38.80486746253305	40.0	38.0	41.0	35.0	41.0
38-39	38.75684450884566	40.0	38.0	41.0	35.0	41.0
40-41	38.71299362619555	40.0	38.0	41.0	35.0	41.0
42-43	38.647660816173996	40.0	38.0	41.0	35.0	41.0
44-45	38.568532310411854	40.0	38.0	41.0	34.0	41.0
46-47	38.494232340863284	40.0	38.0	41.0	34.0	41.0
48-49	38.411553549007984	40.0	38.0	41.0	34.0	41.0
50-51	38.291206121934906	40.0	38.0	41.0	34.0	41.0
52-53	38.17675962652417	40.0	37.0	41.0	34.0	41.0
54-55	38.02529415699513	40.0	37.0	41.0	33.0	41.0
56-57	37.85559440354976	40.0	36.5	41.0	33.0	41.0
58-59	37.651257005714456	39.0	36.0	41.0	33.0	41.0
60-61	37.43620580673705	39.0	35.5	41.0	33.0	41.0
62-63	37.195133328807984	39.0	35.0	40.5	33.0	41.0
64-65	37.174322180598146	38.5	35.0	40.5	33.0	41.0
66-67	36.94640882903745	38.0	35.0	40.0	33.0	41.0
68-69	36.65815196388807	37.0	35.0	40.0	32.5	41.0
70-71	36.355622390393876	37.0	35.0	39.0	32.0	41.0
72-73	36.04293497240972	36.0	35.0	39.0	32.0	41.0
74-75	35.713486816406956	36.0	35.0	39.0	32.0	40.5
76-77	35.39334751971121	35.0	35.0	37.5	32.0	39.5
78-79	35.11010762403187	35.0	35.0	37.0	31.5	39.0
80-81	34.83951073821274	35.0	34.5	36.5	31.0	39.0
82-83	34.60225370086449	35.0	34.0	36.0	31.0	37.5
84-85	34.38646332544959	35.0	34.0	36.0	31.0	37.0
86-87	34.19967528929227	35.0	34.0	35.5	31.0	37.0
88-89	34.06663824189675	35.0	34.0	35.0	31.0	36.0
90-91	33.95719702973379	35.0	34.0	35.0	31.0	36.0
92-93	33.88406698446522	35.0	34.0	35.0	31.0	36.0
94-95	33.83557758579978	35.0	34.0	35.0	31.0	36.0
96-97	33.833096129018784	35.0	34.0	35.0	31.0	35.5
98-99	33.870719809675776	35.0	34.0	35.0	31.0	35.0
100	33.297469069240876	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	5.0
17	227.0
18	1926.0
19	7139.0
20	14270.0
21	22921.0
22	32861.0
23	44103.0
24	59843.0
25	79510.0
26	103587.0
27	133081.0
28	165066.0
29	204525.0
30	251176.0
31	314157.0
32	399944.0
33	531182.0
34	766568.0
35	1177085.0
36	2047519.0
37	4312817.0
38	6048947.0
39	3124673.0
40	160777.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.53749841905908	18.75196399103975	10.184099008643809	27.526438581257363
2	14.602968976192557	21.51427864653273	38.65541370309318	25.22733867418153
3	18.11006948141638	26.726934884230136	28.468894331158257	26.69410130319523
4	12.28210691862414	18.57951235021362	37.363539768860136	31.774840962302108
5	13.343906266324934	38.61408094717483	34.05549715030711	13.986515636193126
6	29.62370403878539	38.46449781709166	18.228224561883465	13.68357358223948
7	26.72729294332377	33.477056518242975	21.972034147600844	17.823616390832406
8	25.507448293858552	36.31739494928741	20.03952227339555	18.135634483458485
9	26.741976943507545	16.559577602578695	20.088557686972198	36.60988776694156
10-11	24.79099586030931	26.72980182374346	27.81058553052878	20.66861678541845
12-13	25.806734783349857	24.38287314829951	28.081437578953317	21.728954489397324
14-15	22.918756883029367	25.143501945369678	26.148897890462415	25.788843281138536
16-17	22.115474429599367	28.222628381899824	26.786453471911642	22.87544371658917
18-19	22.14842998193853	27.28405846656979	28.76451153799432	21.80300001349736
20-21	23.191799882734024	26.396841569663863	28.586222372798293	21.825136174803816
22-23	22.882864680423644	26.335474219092575	28.46816400326248	22.3134970972213
24-25	22.55451075594834	26.508724529848937	28.59613570848874	22.34062900571398
26-27	22.494114654931977	26.650922308584956	28.472275928054952	22.38268710842811
28-29	22.393691565000136	26.610043119828354	28.622289239339672	22.373976075831845
30-31	22.77511993199035	26.48520833554608	28.473800594097646	22.265871138365924
32-33	22.44553043028581	26.680287256781078	28.532641885033172	22.34154042789994
34-35	22.567435836241707	26.63191166550029	28.56629754667315	22.23435495158485
36-37	22.624145871240454	26.484476163901565	28.538763126856953	22.352614838001024
38-39	22.448267148712194	26.6559430940864	28.56372873933155	22.332061017869854
40-41	22.673912747989274	26.524830279766853	28.402584522868857	22.398672449375017
42-43	22.650740967718107	26.58540899859138	28.203491241462373	22.560358792228136
44-45	22.68833745959168	26.878130434594492	27.93547012576969	22.49806198004414
46-47	22.89164184578404	26.829712983188163	27.432896181883404	22.845748989144386
48-49	22.83516421106167	26.964498176708908	27.37902077776524	22.821316834464184
50-51	22.73318978126385	27.12631005208831	27.28011469147408	22.860385475173764
52-53	22.900794557765945	27.031552727126073	27.20204259361674	22.86561012149125
54-55	22.885482039365584	26.889435250058607	27.24714347914039	22.977939231435414
56-57	22.943451515278973	27.074232002769737	27.156504820529488	22.825811661421803
58-59	22.85175066522564	27.202204983747308	27.223935259959305	22.72210909106775
60-61	22.98328029499419	27.219720938879487	27.066290340064604	22.730708426061717
62-63	22.899729362130543	27.33201276278538	27.014623150776316	22.75363472430776
64-65	22.86431713565913	27.239056103185742	27.017041115295164	22.879585645859958
66-67	22.946862056233076	27.28535353067954	26.88067801558135	22.88710639750603
68-69	22.82199813628656	27.19524336680264	27.05706725018989	22.925691246720913
70-71	22.913336760429	26.88059266672816	27.08684146245584	23.119229110386996
72-73	22.985531502308007	26.86954375826559	27.089139898790982	23.055784840635418
74-75	22.754217268733633	27.119692769743832	27.135349016105714	22.990740945416817
76-77	22.940597748676847	27.066930950197275	26.9767500049701	23.015721296155775
78-79	22.920048191087094	27.137061999124573	26.910895816352664	23.03199399343567
80-81	22.930852424796235	27.155211244410477	26.954097541204835	22.959838789588456
82-83	22.943036360200377	27.05007167218625	27.117305015818836	22.889586951794534
84-85	23.08236943155279	27.07435689505021	26.94726682651322	22.896006846883772
86-87	22.794636747220174	27.200401497516257	27.081092138985923	22.923869616277646
88-89	22.92344551630887	27.080486317360318	26.974344101443005	23.0217240648878
90-91	22.997302101169918	27.07872346494065	27.015370681353083	22.908603752536344
92-93	22.991854622373133	27.07973291368015	27.05187427180874	22.87653819213798
94-95	22.789752286620047	27.1497503048843	27.12495251547856	22.93554489301709
96-97	22.926276402170178	27.243814649608243	27.12472283977653	22.705186108445044
98-99	23.921730710985496	29.30018497215195	27.21419973896702	19.563884577895536
100	23.02518185314829	30.156507403545863	20.980694658464426	25.837616084841418
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14569.0
1	12620.0
2	7561.5
3	4275.0
4	4331.5
5	6079.0
6	9264.0
7	15135.0
8	24555.0
9	30865.5
10	30719.0
11	29247.5
12	32277.0
13	42230.0
14	66882.0
15	102011.5
16	130879.0
17	147252.5
18	148092.0
19	134922.0
20	113613.5
21	90739.0
22	75122.0
23	72684.0
24	82318.5
25	102920.5
26	132098.0
27	165492.5
28	197210.0
29	232155.5
30	270692.0
31	307659.0
32	349294.0
33	393226.5
34	436494.5
35	476722.0
36	522929.5
37	569974.5
38	602143.5
39	627424.0
40	656743.0
41	684904.5
42	710807.0
43	739156.5
44	766949.0
45	804498.5
46	834085.5
47	851404.5
48	865348.5
49	863852.5
50	858151.0
51	851696.5
52	830346.0
53	794129.0
54	756393.0
55	708976.0
56	657325.0
57	600680.5
58	540736.5
59	484259.0
60	411582.5
61	335406.5
62	266646.5
63	205502.5
64	155270.5
65	114222.5
66	83913.5
67	63679.0
68	48338.0
69	35162.5
70	25238.0
71	17948.0
72	12294.5
73	8065.5
74	5234.5
75	3254.0
76	2063.5
77	1324.5
78	866.5
79	563.5
80	382.0
81	269.0
82	203.5
83	145.5
84	97.5
85	67.0
86	45.5
87	35.0
88	27.0
89	19.5
90	15.5
91	10.5
92	6.0
93	3.0
94	1.5
95	4.0
96	4.0
97	2.5
98	5.5
99	12.0
100	23.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007968442169555853
3	0.0
4	2.4995113455319487E-5
5	0.0
6	0.010767894876551633
7	0.009008238889297143
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	4.749071556510702E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.509026718252873E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	1.4889630085220798E-4
50-51	0.01416869043331309
52-53	2.917395801764792E-4
54-55	0.0
56-57	0.007910169409141854
58-59	0.024132482664009636
60-61	0.00461411563725181
62-63	0.0012228394320393243
64-65	0.001960795393543502
66-67	0.0010194094709005722
68-69	0.001588007961174957
70-71	1.73875954981081E-5
72-73	0.0054206779391038585
74-75	0.0017418449604136997
76-77	0.01948731515127314
78-79	7.791697478834453E-5
80-81	2.9343238723370673E-4
82-83	0.004059863961870637
84-85	0.00300038644237303
86-87	0.003392804105280517
88-89	6.288418107864669E-6
90-91	6.35944384247009E-6
92-93	0.0
94-95	0.001849281412295236
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	4280.0
20-21	10755.0
22-23	13556.0
24-25	13452.0
26-27	15416.0
28-29	23392.0
30-31	40893.0
32-33	48249.0
34-35	64734.0
36-37	100546.0
38-39	138262.0
40-41	139902.0
42-43	160076.0
44-45	195922.0
46-47	187390.0
48-49	163750.0
50-51	141949.0
52-53	127227.0
54-55	131289.0
56-57	137902.0
58-59	141603.0
60-61	142606.0
62-63	131373.0
64-65	134998.0
66-67	145807.0
68-69	151435.0
70-71	160840.0
72-73	138686.0
74-75	134281.0
76-77	141089.0
78-79	153338.0
80-81	162008.0
82-83	155122.0
84-85	149998.0
86-87	157511.0
88-89	172440.0
90-91	189934.0
92-93	197394.0
94-95	236376.0
96-97	664880.0
98-99	1254993.0
100-101	1.3228256E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.38514857341406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.94199740364637	66.50634237777139
2	8.701043646062532	13.466631105886185
3	2.600941777017008	6.038227975357821
4	1.1354366054841183	3.5146372164432567
5	0.5913442048524643	2.2880629575267672
6	0.31404957999838506	1.4581664044555986
7	0.1935966210881556	1.0487052300352504
8	0.12810365160423637	0.7930656089752562
9	0.0829731258667311	0.5778798905517814
>10	0.30204210132996956	3.580236146575144
>50	0.0056714582869289015	0.30516503390638544
>100	0.0026914729780664923	0.3300441508254064
>500	7.741470111486872E-5	0.04035786998209079
>1k	3.0937083956066944E-5	0.05247803170770771
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.998045382127794E-6
2	4.999022691063897E-6	2.4995113455319484E-5	0.0	0.0	9.998045382127794E-6
3	4.999022691063897E-6	2.4995113455319484E-5	0.0	4.999022691063897E-6	9.998045382127794E-6
4	4.999022691063897E-6	9.498143113021404E-5	0.0	1.4997068073191692E-5	1.4997068073191692E-5
5	4.999022691063897E-6	9.998045382127794E-5	0.0	1.9996090764255588E-5	1.4997068073191692E-5
6	4.999022691063897E-6	9.998045382127794E-5	0.0	1.9996090764255588E-5	3.9992181528511176E-5
7	4.999022691063897E-6	9.998045382127794E-5	0.0	2.4995113455319484E-5	3.9992181528511176E-5
8	4.999022691063897E-6	1.0497947651234183E-4	0.0	3.499315883744728E-5	4.499120421957507E-5
9	4.999022691063897E-6	1.8996286226042808E-4	0.0	7.998436305702235E-5	5.998827229276677E-5
10-11	4.999022691063897E-6	1.8996286226042808E-4	0.0	8.498338574808625E-5	5.998827229276677E-5
12-13	4.999022691063897E-6	1.8996286226042808E-4	0.0	1.1997654458553353E-4	6.998631767489456E-5
14-15	9.998045382127794E-6	2.749462480085143E-4	0.0	1.2497556727659743E-4	1.1247801054893768E-4
16-17	1.749657941872364E-5	2.849442933906421E-4	0.0	1.4497165804085301E-4	1.4747116938638495E-4
18-19	1.9996090764255588E-5	3.1243891819149355E-4	0.0	1.599687261140447E-4	1.8246432822383225E-4
20-21	2.4995113455319484E-5	3.499315883744728E-4	0.0	1.599687261140447E-4	2.0246041898808783E-4
22-23	2.4995113455319484E-5	3.799257245208562E-4	0.0	2.074594416791517E-4	2.374535778255351E-4
24-25	3.499315883744728E-5	4.074203493217076E-4	0.0	2.324545551344712E-4	2.9244282742723796E-4
26-27	3.749267018297923E-5	4.2491692874043125E-4	0.0	2.774457593540463E-4	3.1493842953702554E-4
28-29	3.9992181528511176E-5	4.274164400859632E-4	0.0	4.474125308502188E-4	3.1993745222808946E-4
30-31	3.9992181528511176E-5	4.924037350697939E-4	0.0	0.0011022845033795893	3.299354976102172E-4
32-33	4.2491692874043124E-5	5.049012917974536E-4	0.0	0.0025370040157149274	3.424330543378769E-4
34-35	4.499120421957507E-5	6.023822342731996E-4	0.0	0.004374144854680909	3.499315883744728E-4
36-37	4.499120421957507E-5	7.048621994400095E-4	0.0	0.006948641540578816	3.549306110655367E-4
38-39	4.499120421957507E-5	7.423548696229887E-4	0.0	0.011772698437455478	3.6742816779319643E-4
40-41	4.499120421957507E-5	8.073421646068193E-4	0.0	0.016966683013470867	3.8992376990298395E-4
42-43	4.499120421957507E-5	8.573323915174584E-4	0.0	0.021478300992156033	3.974223039395798E-4
44-45	4.499120421957507E-5	9.298182205378848E-4	0.0	0.0261148945381178	4.2241741739489926E-4
46-47	4.499120421957507E-5	9.723099134119279E-4	0.0	0.03111141771783616	4.524115535412827E-4
48-49	4.999022691063897E-5	9.873069814851196E-4	0.0	0.03605295164795283	4.84905201033198E-4
50-51	4.999022691063897E-5	0.0011597732643268241	0.0	0.04132692058702524	4.84905201033198E-4
52-53	4.999022691063897E-5	0.001164772287017888	0.0	0.04715078202211467	5.248973825617092E-4
54-55	4.999022691063897E-5	0.0011847683777821436	0.0	0.05257972066461007	5.748876094723481E-4
56-57	4.999022691063897E-5	0.001219761536619591	0.0	0.057803699376771836	5.848856548544759E-4
58-59	4.999022691063897E-5	0.0012222610479651229	0.0	0.06293519616914893	5.898846775455398E-4
60-61	4.999022691063897E-5	0.0012297595820017187	0.0	0.067516800465509	6.048817456187315E-4
62-63	4.999022691063897E-5	0.0012372581160383146	0.0	0.07143103523261202	6.298768590740511E-4
64-65	4.999022691063897E-5	0.0012697517635302299	0.0	0.07447793956281547	6.323763704195831E-4
66-67	4.999022691063897E-5	0.0012747507862212936	0.0	0.07751734535898232	6.398749044561788E-4
68-69	4.999022691063897E-5	0.0012997458996766133	0.0	0.0802068195667747	6.473734384927747E-4
70-71	4.999022691063897E-5	0.0013222415017864007	0.0	0.08222392522261898	6.673695292570303E-4
72-73	4.999022691063897E-5	0.0013322395471685286	0.0	0.08405106801620282	7.098612221310734E-4
74-75	5.4989249601702864E-5	0.001362233683314912	0.0	0.08541580121086328	7.173597561676692E-4
76-77	5.4989249601702864E-5	0.0014472170690629982	0.0	0.08672804466726755	7.223587788587331E-4
78-79	5.4989249601702864E-5	0.0014797107165549135	0.0	0.08756788047936628	7.24858290204265E-4
80-81	5.4989249601702864E-5	0.0014797107165549135	0.0	0.08786282281813905	7.523529150051166E-4
82-83	5.4989249601702864E-5	0.0014922082732825733	0.0	0.08825274658804204	7.798475398059679E-4
84-85	5.4989249601702864E-5	0.0014997068073191692	0.0	0.08845770651837566	7.873460738425638E-4
86-87	5.4989249601702864E-5	0.0015346999661566166	0.0	0.08855018843816034	7.948446078791596E-4
88	5.4989249601702864E-5	0.0016746726015064055	0.0	0.08855768697219693	7.998436305702235E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	32335	0.0	52.00926	1
GTATCAA	59935	0.0	39.160282	1
ATCAACG	73880	0.0	31.119635	3
TCAACGC	74250	0.0	30.941586	4
TATCAAC	75795	0.0	30.628416	2
CAACGCA	74990	0.0	30.60782	5
AACGCAG	77415	0.0	29.753708	6
ACGCAGA	88430	0.0	25.991495	7
CGCAGAG	89745	0.0	25.58966	8
GCAGAGT	103655	0.0	22.00345	9
GTACATG	75030	0.0	21.69203	1
TACATGG	74115	0.0	21.597288	2
ACATGGG	75575	0.0	20.70435	3
GAGTACT	59395	0.0	20.477842	12-13
CAGAGTA	99860	0.0	19.504866	10-11
GTACTTT	63635	0.0	19.096157	14-15
AGAGTAC	93585	0.0	18.96288	10-11
CATGGGG	53530	0.0	18.833986	4
CTTATAC	11870	0.0	18.109505	1
AGTACTT	63160	0.0	18.08248	12-13
>>END_MODULE