FastQCFastQC Report
Fri 27 May 2016
SRR2140067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5513473
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64850.045.1381151
GTATCAA110850.035.4915121
ATCAACG126200.030.4628893
TCAACGC126600.030.299514
CAACGCA131000.029.3138545
AACGCAG135500.028.4017686
TATCAAC137800.028.0239092
ACGCAGA154500.024.9366077
CGCAGAG156000.024.6694818
GCAGAGT189550.020.2805219
GAGTACT114400.018.8898112-13
GTACATG130250.018.1821211
CAGAGTA185650.017.93717610-11
TACATGG131100.017.803852
AGAGTAC170400.017.77726410-11
ACATGGG131750.017.002563
GTACTTT129400.016.683714-15
GTGGTAT33200.016.3228111
TATGCCG27250.016.1450642-43
CTTATAC51000.016.0642451
Produced by FastQC (version 0.11.5)