##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140067_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5513473
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.666153439039242	31.0	18.0	33.0	18.0	33.0
2	30.455998786971477	32.0	29.0	33.0	25.0	33.0
3	31.449065951715006	33.0	31.0	33.0	29.0	33.0
4	32.45725643346761	33.0	33.0	33.0	31.0	34.0
5	32.92681763382173	33.0	33.0	34.0	32.0	34.0
6	37.08753176083387	38.0	37.0	38.0	36.0	38.0
7	37.20852845384388	38.0	38.0	38.0	36.0	38.0
8	37.43151476392466	38.0	38.0	38.0	37.0	38.0
9	37.47958319556476	38.0	38.0	38.0	37.0	38.0
10-11	37.49887765841966	38.0	38.0	38.0	37.0	38.0
12-13	37.52014728284695	38.0	38.0	38.0	37.0	38.0
14-15	37.52603377217953	38.0	38.0	38.0	37.0	38.0
16-17	37.46411481474563	38.0	38.0	38.0	37.0	38.0
18-19	37.494798287758	38.0	38.0	38.0	37.0	38.0
20-21	37.519260636623564	38.0	38.0	38.0	37.0	38.0
22-23	37.52800346800598	38.0	38.0	38.0	37.5	38.0
24-25	37.52318350070378	38.0	38.0	38.0	38.0	38.0
26-27	37.492166295769415	38.0	38.0	38.0	37.0	38.0
28-29	37.463506887245714	38.0	38.0	38.0	37.0	38.0
30-31	37.36917949534179	38.0	38.0	38.0	37.0	38.0
32-33	37.43088807862078	38.0	38.0	38.0	37.0	38.0
34-35	37.438589792741716	38.0	38.0	38.0	37.0	38.0
36-37	37.42115955938515	38.0	38.0	38.0	37.0	38.0
38-39	37.39578804229603	38.0	38.0	38.0	37.0	38.0
40-41	37.389681402501154	38.0	38.0	38.0	37.0	38.0
42-43	37.36910887152255	38.0	38.0	38.0	37.0	38.0
44-45	37.33852860886881	38.0	38.0	38.0	37.0	38.0
46-47	37.31455030973389	38.0	38.0	38.0	37.0	38.0
48-49	37.287423957168805	38.0	38.0	38.0	37.0	38.0
50-51	37.248558038914965	38.0	38.0	38.0	37.0	38.0
52-53	37.22816554621335	38.0	38.0	38.0	37.0	38.0
54-55	37.21454905549152	38.0	38.0	38.0	37.0	38.0
56-57	37.206183800290745	38.0	38.0	38.0	37.0	38.0
58-59	37.1954513711037	38.0	38.0	38.0	37.0	38.0
60-61	37.19515008245078	38.0	38.0	38.0	37.0	38.0
62-63	37.20200832482086	38.0	38.0	38.0	37.0	38.0
64-65	37.20454694526521	38.0	38.0	38.0	37.0	38.0
66-67	37.1857818207243	38.0	38.0	38.0	37.0	38.0
68-69	37.18679239526625	38.0	38.0	38.0	36.5	38.0
70-71	37.169081403597566	38.0	38.0	38.0	36.0	38.0
72-73	37.162934168917175	38.0	38.0	38.0	36.0	38.0
74-75	37.1519765339433	38.0	38.0	38.0	36.0	38.0
76-77	37.1550754272056	38.0	38.0	38.0	36.0	38.0
78-79	37.13972357463786	38.0	38.0	38.0	36.0	38.0
80-81	37.13872937945447	38.0	38.0	38.0	36.0	38.0
82-83	37.08601422367168	38.0	38.0	38.0	36.0	38.0
84-85	37.07593632079855	38.0	38.0	38.0	36.0	38.0
86-87	37.073603937518925	38.0	38.0	38.0	36.0	38.0
88-89	37.07941694030833	38.0	38.0	38.0	36.0	38.0
90-91	37.07085412832015	38.0	38.0	38.0	36.0	38.0
92-93	37.05645656487262	38.0	38.0	38.0	36.0	38.0
94-95	37.02700329577539	38.0	38.0	38.0	36.0	38.0
96-97	36.99220652720694	38.0	38.0	38.0	36.0	38.0
98-99	36.99825216781305	38.0	38.0	38.0	36.0	38.0
100	35.47419140921428	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	9.0
19	15.0
20	41.0
21	103.0
22	341.0
23	818.0
24	1979.0
25	3852.0
26	7084.0
27	11583.0
28	17922.0
29	26632.0
30	36421.0
31	48366.0
32	62594.0
33	84226.0
34	126483.0
35	233196.0
36	764366.0
37	4087439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.06400321539618	21.342718101639385	14.267377386268146	24.32590129669629
2	14.005214163238897	20.9786904226164	41.81038446699574	23.20571094714896
3	17.48358974461288	26.058892462155885	31.30366286367957	25.15385492955166
4	12.344640664056357	18.438069478586836	39.54359547614871	29.673694381208094
5	13.19182325484547	37.499650843941936	35.94123585645895	13.367290044753643
6	29.23819523556205	38.56298924471018	19.11022326580723	13.088592253920533
7	25.75875496261612	32.92349486430785	23.698184429306174	17.61956574376985
8	23.596506231190396	37.26163164306781	21.71458897141602	17.427273154325775
9	25.083259906940437	17.022003250954302	21.848901360997036	36.04583548110823
10-11	23.616630201222865	27.0514918184458	29.55687910939071	19.774998870940625
12-13	24.444558306947208	24.570205074932357	29.54561757585623	21.439619042264198
14-15	21.70322589772363	26.0199424210475	27.109437191403675	25.167394489825195
16-17	21.389376532722657	29.100187848929345	27.823696606476535	21.686739011871463
18-19	21.34870343973753	28.299322405315124	29.436663605680124	20.91531054926722
20-21	22.11139206343247	27.493197924834277	29.268484676671765	21.12692533506149
22-23	21.824631698088567	27.496462995679412	29.092014713937974	21.586890592294047
24-25	21.64686795278493	27.557522326157812	29.247892547112038	21.547717173945223
26-27	21.659230913383105	27.771418298356902	29.079993563521494	21.4893572247385
28-29	21.523669545092456	27.75389600119702	29.161507824849604	21.56092662886092
30-31	21.95790683432136	27.559489129532345	29.04993070100714	21.432673335139157
32-33	21.721448276226273	27.738972733965824	29.03190090852788	21.50767808128002
34-35	21.769791493798955	27.778112159934487	29.061055817292047	21.391040528974507
36-37	21.77330933197137	27.652480927183344	29.06276430772031	21.511445433124983
38-39	21.574339958781525	27.697910805824655	29.216533257206635	21.511215978187188
40-41	21.83417353313459	27.558792064329683	29.077342473110836	21.529691929424892
42-43	21.751463769163013	27.618322822445673	28.972288241519202	21.65792516687212
44-45	21.75207452274115	27.735191578469166	28.8690536550266	21.643680243763082
46-47	21.859235336772347	27.6783289900727	28.59553883794389	21.866896835211065
48-49	21.78773229029638	27.744199948525267	28.66206683632273	21.806000924855624
50-51	21.730476999823843	27.883573404467434	28.59045884204474	21.79549075366398
52-53	21.875339171991758	27.800562334363395	28.56230666891733	21.76179182472752
54-55	21.85035651225416	27.743391734755157	28.54593358720814	21.860318165782544
56-57	21.899803365168506	27.825422725283516	28.39582215339893	21.878951756149046
58-59	21.749281874492222	27.955592995007557	28.58546741536699	21.709657715133236
60-61	21.902324297612573	28.001946482400204	28.451637851636463	21.64409136835076
62-63	21.7994981498664	28.091463982503623	28.41789787408622	21.691139993543757
64-65	21.74844816379092	28.069407113557034	28.41483248312003	21.767312239532025
66-67	21.79371633505764	28.136911212861275	28.229989317230764	21.839383134850323
68-69	21.834960722429223	28.058086341344495	28.27050176212037	21.836451174105914
70-71	21.833236316095846	27.80494377386177	28.343826282653843	22.017993627388545
72-73	21.90552573257504	27.74077114236846	28.362806643922404	21.990896481134094
74-75	21.84928172185443	27.827584057016274	28.244071741216192	22.079062479913098
76-77	21.96059093991601	27.758473411352785	28.182267146516516	22.09866850221469
78-79	21.964755136883543	27.83434081402458	28.11069523983273	22.090208809259153
80-81	21.98086526273152	27.824018250678634	28.12276605501803	22.07235043157182
82-83	22.029055372763395	27.7860277551377	28.171919424651552	22.012997447447347
84-85	22.136314381525978	27.81424108347594	28.055946173568934	21.993498361429147
86-87	21.949109166825373	27.811992618815708	28.143042073182016	22.095856141176903
88-89	22.051743039762805	27.831578734893103	28.04950959821334	22.067168627130755
90-91	22.091333580096308	27.825873387424238	28.108295348633796	21.974497683845655
92-93	22.156150944774144	27.821062403358614	28.058478951781247	21.964307700085996
94-95	22.074670646691313	27.94096060203787	28.047936775709264	21.93643197556155
96-97	22.114111228873988	28.015372559381014	28.136347395822387	21.734168815922608
98-99	23.186519663707266	29.877666943859506	28.390640165060688	18.545173227372548
100	22.016530733628215	30.88107853123953	22.314767592105174	24.78762314302708
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	826.0
1	796.0
2	1115.5
3	1624.0
4	2067.0
5	2721.0
6	3332.0
7	4216.5
8	5418.0
9	6359.0
10	6715.0
11	6830.5
12	7510.5
13	8958.0
14	11680.5
15	14731.5
16	16592.5
17	17453.5
18	17470.0
19	16716.5
20	16069.5
21	16375.0
22	18282.0
23	22256.0
24	28732.0
25	37721.5
26	49509.0
27	62934.5
28	75802.5
29	89482.5
30	102794.0
31	115091.5
32	128242.0
33	141595.0
34	154446.0
35	166213.0
36	179222.0
37	191423.5
38	199793.0
39	206780.5
40	214363.0
41	220853.5
42	225486.0
43	230977.5
44	237778.5
45	243678.0
46	241896.5
47	237362.0
48	233720.5
49	226457.5
50	218390.0
51	208436.0
52	195639.0
53	182970.5
54	171068.5
55	158447.0
56	144654.5
57	128941.0
58	113745.5
59	98761.0
60	81958.5
61	65760.0
62	51466.0
63	39559.5
64	30017.5
65	22258.0
66	16210.0
67	12233.5
68	9239.0
69	6651.5
70	4732.5
71	3342.5
72	2338.0
73	1567.5
74	1022.5
75	658.5
76	424.0
77	266.5
78	160.5
79	88.0
80	56.5
81	39.5
82	25.0
83	17.0
84	12.5
85	9.5
86	8.0
87	6.0
88	4.0
89	2.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007472603928594554
3	0.0
4	0.003736301964297277
5	0.003246592483539867
6	0.0
7	0.0
8	0.0
9	2.720608226430056E-4
10-11	6.166711979908127E-4
12-13	9.068694088100187E-6
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.703994608569426E-4
24-25	0.0014602124309788874
26-27	0.0
28-29	0.002005272869547291
30-31	0.0029884240804487396
32-33	6.009348360831869E-4
34-35	0.0
36-37	0.0010825633448638562
38-39	0.002225031568212405
40-41	0.0015523152549645272
42-43	0.0
44-45	0.0
46-47	0.0013704852859845894
48-49	4.607937037148325E-4
50-51	0.001693793987224922
52-53	0.0025938518495187577
54-55	0.001876370929650433
56-57	0.001821759812057753
58-59	0.0019063149026616647
60-61	8.553018450772649E-4
62-63	6.086929880393856E-4
64-65	0.003324631664057939
66-67	0.0025083078666108716
68-69	9.380456298747105E-5
70-71	0.002989374877504049
72-73	0.009645421747926953
74-75	0.012242457744744006
76-77	0.00501307296825458
78-79	0.004547037960006397
80-81	3.4214386906882563E-4
82-83	7.800512805711848E-5
84-85	6.749334768691862E-5
86-87	0.0021463072953325653
88-89	0.004484114651127852
90-91	5.792524515411881E-4
92-93	0.0010181913340682257
94-95	0.0025548720878450183
96-97	0.0024739600690835335
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	52.0
20-21	161.0
22-23	294.0
24-25	435.0
26-27	1339.0
28-29	3648.0
30-31	12529.0
32-33	15754.0
34-35	21562.0
36-37	32712.0
38-39	37077.0
40-41	35841.0
42-43	40242.0
44-45	46402.0
46-47	46012.0
48-49	43274.0
50-51	39523.0
52-53	37263.0
54-55	39614.0
56-57	39954.0
58-59	40456.0
60-61	40838.0
62-63	40329.0
64-65	42776.0
66-67	46164.0
68-69	47019.0
70-71	49790.0
72-73	42138.0
74-75	41797.0
76-77	43000.0
78-79	44072.0
80-81	43879.0
82-83	42213.0
84-85	41941.0
86-87	42739.0
88-89	43676.0
90-91	43892.0
92-93	43715.0
94-95	52190.0
96-97	165662.0
98-99	364674.0
100-101	3656825.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.52573310494279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.15424072327978	79.78341744929494
2	6.447326814656744	11.286140120399743
3	1.40020101312033	3.676608605029215
4	0.48608638095089185	1.701802673802212
5	0.20023914917042404	0.8763039163725682
6	0.1039027378670834	0.5456497982056305
7	0.06307327295954428	0.3864374118578595
8	0.03525498561621848	0.2468574769330989
9	0.025732242439760387	0.20270100455767348
>10	0.08176919319211967	1.0591214534356412
>50	0.0013686653020382855	0.0765501281406431
>100	7.446915864203594E-4	0.11591196410920214
>500	6.012985860797284E-5	0.04249799786155979
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.8137388176200374E-5	0.0	0.0	0.0
3	0.0	1.8137388176200374E-5	0.0	0.0	0.0
4	0.0	9.068694088100187E-5	0.0	0.0	0.0
5	0.0	9.068694088100187E-5	0.0	0.0	0.0
6	0.0	9.068694088100187E-5	0.0	0.0	0.0
7	0.0	9.068694088100187E-5	0.0	0.0	0.0
8	0.0	9.068694088100187E-5	0.0	0.0	0.0
9	0.0	1.6323649358580337E-4	0.0	0.0	0.0
10-11	0.0	1.6323649358580337E-4	0.0	0.0	0.0
12-13	0.0	1.6323649358580337E-4	0.0	0.0	0.0
14-15	0.0	1.995112699382041E-4	0.0	9.068694088100187E-6	0.0
16-17	0.0	1.995112699382041E-4	0.0	1.8137388176200374E-5	0.0
18-19	0.0	2.0857996402630428E-4	0.0	1.8137388176200374E-5	0.0
20-21	0.0	2.3578604629060485E-4	0.0	3.627477635240075E-5	0.0
22-23	0.0	2.9926690490730617E-4	0.0	6.34808586167013E-5	0.0
24-25	0.0	3.536790694359073E-4	0.0	9.068694088100187E-5	0.0
26-27	0.0	3.627477635240075E-4	0.0	1.7230518767390353E-4	0.0
28-29	0.0	3.808851517002078E-4	0.0	5.531903393741113E-4	0.0
30-31	0.0	4.534347044050093E-4	0.0	0.0017230518767390355	0.0
32-33	0.0	4.534347044050093E-4	0.0	0.004634102679019195	9.068694088100187E-6
34-35	0.0	5.259842571098108E-4	0.0	0.00825251162017117	1.8137388176200374E-5
36-37	0.0	5.622590334622116E-4	0.0	0.013739071543471782	1.8137388176200374E-5
38-39	0.0	6.438772802551133E-4	0.0	0.027795547380027073	1.8137388176200374E-5
40-41	0.0	6.892207506956142E-4	0.0	0.043266739494325995	1.8137388176200374E-5
42-43	0.0	7.073581388718146E-4	0.0	0.05258028832280488	1.8137388176200374E-5
44-45	0.0	7.980450797528164E-4	0.0	0.0630546299945606	1.8137388176200374E-5
46-47	0.0	8.887320206338183E-4	0.0	0.07493461924997184	1.8137388176200374E-5
48-49	0.0	8.887320206338183E-4	0.0	0.08593494517883737	1.8137388176200374E-5
50-51	0.0	9.794189615148201E-4	0.0	0.09741591189437221	1.8137388176200374E-5
52-53	0.0	9.794189615148201E-4	0.0	0.12853967000473204	1.8137388176200374E-5
54-55	0.0	9.794189615148201E-4	0.0	0.16775270324167724	1.8137388176200374E-5
56-57	0.0	0.001015693737867221	0.0	0.21712267385729467	1.8137388176200374E-5
58-59	0.0	0.0010610372083077217	0.0	0.2529168094230261	1.8137388176200374E-5
60-61	0.0	0.0011063806787482227	0.0	0.26861471888952754	1.8137388176200374E-5
62-63	0.0	0.0011335867610125232	0.0	0.2824082025975279	1.8137388176200374E-5
64-65	0.0	0.001215205007805425	0.0	0.2887653571532861	1.8137388176200374E-5
66-67	0.0	0.0012242737018935252	0.0	0.295086236932692	1.8137388176200374E-5
68-69	0.0	0.0012514797841578257	0.0	0.3014705975707145	1.8137388176200374E-5
70-71	0.0	0.0012514797841578257	0.0	0.3066125471186673	1.8137388176200374E-5
72-73	0.0	0.0012514797841578257	0.0	0.310566497741079	1.8137388176200374E-5
74-75	0.0	0.0012877545605102267	0.0	0.3139037771654999	1.8137388176200374E-5
76-77	0.0	0.0013512354191269277	0.0	0.3167966905796038	1.8137388176200374E-5
78-79	0.0	0.0013784415013912284	0.0	0.31876459719672157	1.8137388176200374E-5
80-81	0.0	0.0013784415013912284	0.0	0.31947195533559336	1.8137388176200374E-5
82-83	0.0	0.0013965788895674287	0.0	0.31994352742817456	1.8137388176200374E-5
84-85	0.0	0.0014147162777436292	0.0	0.32013397000402466	1.8137388176200374E-5
86-87	0.0	0.0014328536659198295	0.0	0.32022465694490565	1.8137388176200374E-5
88	0.0	0.0015235406068008312	0.0	0.32023372563899377	1.8137388176200374E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6485	0.0	45.138115	1
GTATCAA	11085	0.0	35.491512	1
ATCAACG	12620	0.0	30.462889	3
TCAACGC	12660	0.0	30.29951	4
CAACGCA	13100	0.0	29.313854	5
AACGCAG	13550	0.0	28.401768	6
TATCAAC	13780	0.0	28.023909	2
ACGCAGA	15450	0.0	24.936607	7
CGCAGAG	15600	0.0	24.669481	8
GCAGAGT	18955	0.0	20.280521	9
GAGTACT	11440	0.0	18.88981	12-13
GTACATG	13025	0.0	18.182121	1
CAGAGTA	18565	0.0	17.937176	10-11
TACATGG	13110	0.0	17.80385	2
AGAGTAC	17040	0.0	17.777264	10-11
ACATGGG	13175	0.0	17.00256	3
GTACTTT	12940	0.0	16.6837	14-15
GTGGTAT	3320	0.0	16.322811	1
TATGCCG	2725	0.0	16.14506	42-43
CTTATAC	5100	0.0	16.064245	1
>>END_MODULE