FastQCFastQC Report
Fri 27 May 2016
SRR2140066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4443941
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50000.049.047251
GTATCAA92750.036.9525221
TCAACGC103700.032.4706844
ATCAACG104050.032.361463
CAACGCA107950.031.1919615
AACGCAG112900.030.0869546
TATCAAC114050.029.9019372
ACGCAGA128700.026.2279247
CGCAGAG129600.026.0457868
GCAGAGT157050.021.3849549
TGGTATC25100.020.0156822
GAGTACT92350.019.47417612-13
CAGAGTA153850.018.5649710-11
TACATGG110150.018.5532022
GTGGTAT27600.018.3569281
GTACATG114050.018.2920721
AGAGTAC142250.017.77474610-11
ACATGGG111300.017.6701623
GTACTTT104500.017.21014614-15
AGTACTT100500.016.07366812-13
Produced by FastQC (version 0.11.5)