##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140066_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4443941
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.573477910710334	31.0	18.0	33.0	18.0	33.0
2	30.35672210769675	32.0	28.0	33.0	25.0	33.0
3	31.406332127271718	33.0	31.0	33.0	29.0	33.0
4	32.48747204339571	33.0	33.0	33.0	31.0	34.0
5	32.94737486388771	33.0	33.0	34.0	32.0	34.0
6	37.12152569082263	38.0	37.0	38.0	36.0	38.0
7	37.232020182086124	38.0	38.0	38.0	36.0	38.0
8	37.456006279111264	38.0	38.0	38.0	37.0	38.0
9	37.50539059812	38.0	38.0	38.0	37.0	38.0
10-11	37.52490885905101	38.0	38.0	38.0	37.0	38.0
12-13	37.544715377634404	38.0	38.0	38.0	37.5	38.0
14-15	37.551037243743785	38.0	38.0	38.0	38.0	38.0
16-17	37.48805980997498	38.0	38.0	38.0	37.5	38.0
18-19	37.52021730261495	38.0	38.0	38.0	37.0	38.0
20-21	37.54596428552641	38.0	38.0	38.0	38.0	38.0
22-23	37.55326534014522	38.0	38.0	38.0	38.0	38.0
24-25	37.550322649559384	38.0	38.0	38.0	38.0	38.0
26-27	37.520956756907474	38.0	38.0	38.0	38.0	38.0
28-29	37.492868212294624	38.0	38.0	38.0	37.0	38.0
30-31	37.39807954392289	38.0	38.0	38.0	37.0	38.0
32-33	37.46211420100253	38.0	38.0	38.0	37.0	38.0
34-35	37.47365443806625	38.0	38.0	38.0	37.0	38.0
36-37	37.45751331493045	38.0	38.0	38.0	37.0	38.0
38-39	37.43652928280265	38.0	38.0	38.0	37.0	38.0
40-41	37.42039443308153	38.0	38.0	38.0	37.0	38.0
42-43	37.39840080960237	38.0	38.0	38.0	37.0	38.0
44-45	37.365440651726175	38.0	38.0	38.0	37.0	38.0
46-47	37.340929647144236	38.0	38.0	38.0	37.0	38.0
48-49	37.31384403938279	38.0	38.0	38.0	37.0	38.0
50-51	37.27164486039008	38.0	38.0	38.0	37.0	38.0
52-53	37.25192114575438	38.0	38.0	38.0	37.0	38.0
54-55	37.23926961681431	38.0	38.0	38.0	37.0	38.0
56-57	37.22935797361659	38.0	38.0	38.0	37.0	38.0
58-59	37.21977350511756	38.0	38.0	38.0	37.0	38.0
60-61	37.21833298308387	38.0	38.0	38.0	37.0	38.0
62-63	37.22342768427117	38.0	38.0	38.0	37.0	38.0
64-65	37.22648455009873	38.0	38.0	38.0	37.0	38.0
66-67	37.207496825465654	38.0	38.0	38.0	37.0	38.0
68-69	37.2095910905043	38.0	38.0	38.0	37.0	38.0
70-71	37.19314667715028	38.0	38.0	38.0	36.5	38.0
72-73	37.186581569280044	38.0	38.0	38.0	36.0	38.0
74-75	37.17626911654717	38.0	38.0	38.0	36.0	38.0
76-77	37.179437913724904	38.0	38.0	38.0	36.0	38.0
78-79	37.16232535811952	38.0	38.0	38.0	36.0	38.0
80-81	37.1602857912893	38.0	38.0	38.0	36.0	38.0
82-83	37.10848026470307	38.0	38.0	38.0	36.0	38.0
84-85	37.10063669524905	38.0	38.0	38.0	36.0	38.0
86-87	37.096650061809285	38.0	38.0	38.0	36.0	38.0
88-89	37.10358542072038	38.0	38.0	38.0	36.0	38.0
90-91	37.09366994230346	38.0	38.0	38.0	36.0	38.0
92-93	37.07675060434897	38.0	38.0	38.0	36.0	38.0
94-95	37.048707174581416	38.0	38.0	38.0	36.0	38.0
96-97	37.01530029585314	38.0	38.0	38.0	36.0	38.0
98-99	37.01931421881814	38.0	38.0	38.0	36.0	38.0
100	35.47437232054674	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	2.0
19	12.0
20	20.0
21	80.0
22	277.0
23	691.0
24	1646.0
25	3143.0
26	5515.0
27	9159.0
28	13990.0
29	20394.0
30	28087.0
31	37004.0
32	47324.0
33	64029.0
34	95567.0
35	179450.0
36	608178.0
37	3329370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.954513347499436	21.50579856933294	14.385587027370525	24.154101055797096
2	14.014268566081572	21.04834038240045	41.70744803666565	23.229943014852324
3	17.36568959848927	26.169429342108728	31.444026822138277	25.02085423726373
4	12.392883696898668	18.574529543396505	39.55055709478983	29.482029664915
5	13.18202050579029	37.533175301058684	35.923748452344356	13.361055740806666
6	29.16472113378643	38.694618132869	19.115600319626207	13.025060413718364
7	25.726421660413585	33.0971765826774	23.812939910768392	17.36346184614062
8	23.70080970921981	37.155938838971984	21.690521993878857	17.452729457929347
9	25.12205961608349	17.108778593746308	21.935244327381024	35.833917462789174
10-11	23.566892597152716	27.140743017676854	29.62682596451758	19.665538420652855
12-13	24.379995636755265	24.743558110195217	29.526137894960563	21.350308358088952
14-15	21.63932869495792	26.22315417778949	27.145882449834506	24.991634677418084
16-17	21.33826709220487	29.197732373134567	27.894204716039212	21.569795818621355
18-19	21.275777513697864	28.466725818367077	29.51223924890092	20.74525741903414
20-21	22.048466776682904	27.61254527143395	29.376619288125866	20.962368663757278
22-23	21.801915739420732	27.58703022133508	29.171076452922833	21.439977586321355
24-25	21.5558586009871	27.667221458107132	29.338763695417363	21.438156245488404
26-27	21.564742291244656	27.86414584740041	29.184424938104886	21.386686923250057
28-29	21.437205402917115	27.874821154824502	29.235356029253772	21.452617413004607
30-31	21.754690524870416	27.642051211080776	29.17915448500977	21.42410377903904
32-33	21.521281675247895	27.820461453604047	29.14928761367383	21.50896925747423
34-35	21.66281934698367	27.883648623201886	29.13157833315847	21.32195369665597
36-37	21.657332858676345	27.76370094060384	29.094166908699243	21.484799292020572
38-39	21.504537948787135	27.881029992860316	29.19101439641719	21.423417661935357
40-41	21.77178560397102	27.72604329247701	29.0630755130811	21.43909559047087
42-43	21.7645314773509	27.764256116845726	28.944127124611775	21.5270852811916
44-45	21.748263221295904	27.93188115549175	28.785753956096706	21.53410166711564
46-47	21.77764909018211	27.850733987703457	28.607701244084183	21.763915678030248
48-49	21.75295066630562	27.882645484857793	28.69169982181577	21.672704027020817
50-51	21.736378241220365	28.02431121721244	28.5654159722535	21.673894569313696
52-53	21.883547182717866	27.985882394517652	28.459063264779775	21.671507157984703
54-55	21.83999320917805	27.927621156044747	28.452726829674035	21.779658805103168
56-57	21.86356703848989	28.009029418658614	28.414138251536407	21.713265291315086
58-59	21.792942952886136	28.079710549309965	28.49665903009316	21.63068746771074
60-61	21.891042849215648	28.102122456952998	28.424222254988734	21.58261243884262
62-63	21.820821805649846	28.158766044221156	28.366481900028766	21.65393025010023
64-65	21.83492841133233	28.144026760321346	28.339236781857156	21.681808046489166
66-67	21.855634105669086	28.186921598656706	28.216665661076874	21.740778634597337
68-69	21.85593328202431	28.12080138508442	28.261689501256072	21.761575831635202
70-71	21.839373674289376	27.938133373262257	28.317767945046768	21.9047250074016
72-73	21.930176535828924	27.893213598847254	28.262232168366175	21.914377696957647
74-75	21.827473620215436	27.97028447840111	28.235563509775023	21.96667839160843
76-77	21.940828625565217	27.907366481140077	28.174320003394847	21.97748488989986
78-79	21.904396461930236	27.94616560566755	28.104468442505002	22.044969489897206
80-81	21.905653567561583	27.929994862467233	28.113986706187276	22.050364863783912
82-83	21.957747460341743	27.89136251058798	28.201041044031292	21.949848985038983
84-85	22.042976464188314	27.89273531151089	28.14000633841383	21.924281885886966
86-87	21.89227870723801	27.921456386259752	28.178843195107923	22.007421711394315
88-89	21.98441856648465	27.903941059033254	28.12357319595093	21.988067178531164
90-91	22.02698434825664	27.915591991517903	28.11329944117856	21.9441242190469
92-93	22.05787710852707	27.886587352455177	28.111009688572718	21.944525850445036
94-95	21.928996342748224	27.968191487223265	28.131277957047097	21.97153421298141
96-97	22.023507651471817	28.106041157156564	28.179395599033754	21.691055592337868
98-99	23.104628634090588	29.94044259368767	28.441624710348584	18.51330406187316
100	21.962761926388392	30.901123899833934	22.388601773397937	24.74751240037974
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	882.0
1	820.0
2	989.0
3	1356.0
4	1689.0
5	2178.0
6	2686.0
7	3392.0
8	4256.0
9	4898.5
10	5206.5
11	5439.5
12	6053.0
13	7283.0
14	9626.5
15	12196.5
16	13844.5
17	14617.5
18	14589.5
19	14033.5
20	13461.0
21	13700.0
22	15338.0
23	18519.5
24	23822.0
25	31271.0
26	40832.5
27	51498.5
28	62120.5
29	73422.0
30	84316.5
31	94281.5
32	105387.5
33	116552.0
34	126765.0
35	136838.0
36	147328.0
37	156840.0
38	164037.5
39	170423.0
40	177250.5
41	182437.0
42	185377.0
43	189717.0
44	192849.0
45	194066.5
46	193369.0
47	190489.5
48	186638.5
49	180494.5
50	173868.0
51	166210.0
52	156542.0
53	146270.0
54	135493.5
55	123960.5
56	112370.5
57	100444.0
58	88580.0
59	76469.5
60	63507.0
61	51068.5
62	40003.0
63	30775.5
64	23041.0
65	17002.5
66	12727.5
67	9723.5
68	7433.5
69	5414.5
70	3907.5
71	2859.5
72	2044.5
73	1385.0
74	971.0
75	679.5
76	430.5
77	262.0
78	153.0
79	97.0
80	64.5
81	42.0
82	31.5
83	21.0
84	11.0
85	9.0
86	9.5
87	8.5
88	5.5
89	2.5
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0073583335152289375
3	0.0
4	0.003892940972888704
5	0.00276781352407694
6	0.0
7	0.0
8	0.0
9	1.350152938574117E-4
10-11	5.063073519652938E-4
12-13	1.1251274488117641E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0010239075665756513
24-25	0.0015303322542615534
26-27	0.0
28-29	0.0020939180798455995
30-31	0.00299766272462749
32-33	6.212649270194442E-4
34-35	3.3990247744718734E-5
36-37	0.001206354712442593
38-39	0.0025310079974126456
40-41	0.0015564877059377352
42-43	0.0
44-45	0.0
46-47	0.0014052047129385226
48-49	5.59912548807696E-4
50-51	0.001561466841951329
52-53	0.0023478766541789485
54-55	0.0018171948341419444
56-57	0.0020413664464286416
58-59	0.00171131716361829
60-61	8.254090933819075E-4
62-63	6.81030954748646E-4
64-65	0.003535965796272151
66-67	0.0025673973907540315
68-69	9.072823536563284E-5
70-71	0.003232777378515956
72-73	0.010218623010805566
74-75	0.012568482888050575
76-77	0.005024659899226606
78-79	0.004571714070307248
80-81	3.161125740098564E-4
82-83	1.2493179071259845E-4
84-85	5.606731778577266E-5
86-87	0.001642445783006293
88-89	0.004463984870525124
90-91	6.073480436885692E-4
92-93	0.0010231472447740928
94-95	0.002438699590786209
96-97	0.0024320774805839146
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	34.0
20-21	105.0
22-23	249.0
24-25	381.0
26-27	1150.0
28-29	2980.0
30-31	9797.0
32-33	12387.0
34-35	17309.0
36-37	26374.0
38-39	29325.0
40-41	28967.0
42-43	32929.0
44-45	37972.0
46-47	37938.0
48-49	35059.0
50-51	32017.0
52-53	30976.0
54-55	33742.0
56-57	33831.0
58-59	33807.0
60-61	33768.0
62-63	33246.0
64-65	35322.0
66-67	37091.0
68-69	37404.0
70-71	38648.0
72-73	34987.0
74-75	35322.0
76-77	36724.0
78-79	37070.0
80-81	36372.0
82-83	34724.0
84-85	35650.0
86-87	36488.0
88-89	36773.0
90-91	37100.0
92-93	37094.0
94-95	43105.0
96-97	133866.0
98-99	291754.0
100-101	2924104.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.69851712054724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.59302250087323	81.24165274416373
2	6.239272585511654	11.06828452491533
3	1.3494063336347544	3.5907102235943142
4	0.4228505088175667	1.500248523831482
5	0.16828561896179978	0.7463342427312545
6	0.08297083214175038	0.4415633865138659
7	0.045561865747144686	0.28288889503120307
8	0.02957340745337002	0.20984939098525282
9	0.01867769674725032	0.14910156042375347
>10	0.04921047121097116	0.6277685616128603
>50	7.164596377873299E-4	0.04196428815703474
>100	4.1733533094781236E-4	0.08045039235976352
>500	3.438393181461325E-5	0.01918326568015297
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.5005097952470564E-5	0.0
3	0.0	0.0	0.0	4.5005097952470564E-5	0.0
4	0.0	0.0	0.0	4.5005097952470564E-5	0.0
5	0.0	0.0	0.0	6.750764692870585E-5	0.0
6	0.0	0.0	0.0	6.750764692870585E-5	0.0
7	0.0	0.0	0.0	6.750764692870585E-5	0.0
8	0.0	4.5005097952470564E-5	0.0	6.750764692870585E-5	0.0
9	0.0	1.350152938574117E-4	0.0	9.001019590494113E-5	0.0
10-11	2.2502548976235282E-5	1.350152938574117E-4	0.0	9.001019590494113E-5	0.0
12-13	4.5005097952470564E-5	1.350152938574117E-4	0.0	1.1251274488117642E-4	0.0
14-15	6.750764692870585E-5	2.2502548976235283E-4	0.0	1.350152938574117E-4	1.1251274488117641E-5
16-17	1.1251274488117642E-4	2.700305877148234E-4	0.0	1.350152938574117E-4	2.2502548976235282E-5
18-19	1.350152938574117E-4	2.925331366910587E-4	0.0	1.350152938574117E-4	2.2502548976235282E-5
20-21	1.350152938574117E-4	3.262869601554116E-4	0.0	1.5751784283364698E-4	2.2502548976235282E-5
22-23	1.6876911732176462E-4	3.9379460708411745E-4	0.0	1.9127166629799992E-4	2.2502548976235282E-5
24-25	2.0252294078611753E-4	4.050458815722351E-4	0.0	2.0252294078611756E-4	2.2502548976235282E-5
26-27	2.2502548976235283E-4	4.050458815722351E-4	0.0	2.5877931322670575E-4	2.2502548976235282E-5
28-29	2.2502548976235283E-4	4.275484305484704E-4	0.0	5.963175478702349E-4	2.2502548976235282E-5
30-31	2.4752803873858814E-4	5.400611754296468E-4	0.0	0.0015751784283364698	2.2502548976235282E-5
32-33	2.700305877148234E-4	5.400611754296468E-4	0.0	0.004207976658555998	2.2502548976235282E-5
34-35	2.812818622029411E-4	6.525739203108233E-4	0.0	0.007448343711133879	2.2502548976235282E-5
36-37	2.925331366910587E-4	7.313328417276467E-4	0.0	0.012252637917560111	2.2502548976235282E-5
38-39	2.925331366910587E-4	7.425841162157644E-4	0.0	0.023391399660796577	2.2502548976235282E-5
40-41	2.925331366910587E-4	7.650866651919996E-4	0.0	0.035182735324343864	2.2502548976235282E-5
42-43	2.925331366910587E-4	7.988404886563526E-4	0.0	0.0438124628567301	2.2502548976235282E-5
44-45	2.925331366910587E-4	8.775994100731761E-4	0.0	0.05302725666249844	2.2502548976235282E-5
46-47	3.037844111791763E-4	9.563583314899995E-4	0.0	0.06346843938747163	2.2502548976235282E-5
48-49	3.1503568566729396E-4	9.676096059781172E-4	0.0	0.07331330456457455	2.2502548976235282E-5
50-51	3.1503568566729396E-4	0.001035117252906823	0.0	0.08234807797853302	2.2502548976235282E-5
52-53	3.1503568566729396E-4	0.0010576198018830583	0.0	0.10312918195808629	2.2502548976235282E-5
54-55	3.1503568566729396E-4	0.0010801223508592936	0.0	0.1300984869061043	2.2502548976235282E-5
56-57	3.1503568566729396E-4	0.001102624899835529	0.0	0.1621196140992871	2.2502548976235282E-5
58-59	3.1503568566729396E-4	0.001102624899835529	0.0	0.18862761679329226	3.3753823464352926E-5
60-61	3.1503568566729396E-4	0.001102624899835529	0.0	0.1991700609886585	4.5005097952470564E-5
62-63	3.1503568566729396E-4	0.001102624899835529	0.0	0.2112876836123612	4.5005097952470564E-5
64-65	3.1503568566729396E-4	0.0012151376447167052	0.0	0.21762215114917144	4.5005097952470564E-5
66-67	3.1503568566729396E-4	0.0012376401936929405	0.0	0.22379910084314802	5.625637244058821E-5
68-69	3.1503568566729396E-4	0.0012938965661335286	0.0	0.22967226612594543	6.750764692870585E-5
70-71	3.1503568566729396E-4	0.0013389016640859993	0.0	0.23484785239047953	6.750764692870585E-5
72-73	3.1503568566729396E-4	0.001350152938574117	0.0	0.2385157678736059	1.1251274488117642E-4
74-75	3.1503568566729396E-4	0.0013726554875503523	0.0	0.24181239129862436	1.1251274488117642E-4
76-77	3.1503568566729396E-4	0.001473916957943411	0.0	0.24492899433183296	1.1251274488117642E-4
78-79	3.1503568566729396E-4	0.0014851682324315287	0.0	0.24714549540599212	1.1251274488117642E-4
80-81	3.1503568566729396E-4	0.0014851682324315287	0.0	0.2475842951110287	1.1251274488117642E-4
82-83	3.1503568566729396E-4	0.0014851682324315287	0.0	0.24829312540378012	1.1251274488117642E-4
84-85	3.1503568566729396E-4	0.0014964195069196464	0.0	0.24855190471700683	1.1251274488117642E-4
86-87	3.1503568566729396E-4	0.0015301733303839991	0.0	0.24872067383432858	1.1251274488117642E-4
88	3.1503568566729396E-4	0.0015751784283364698	0.0	0.24874317638330481	1.1251274488117642E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5000	0.0	49.04725	1
GTATCAA	9275	0.0	36.952522	1
TCAACGC	10370	0.0	32.470684	4
ATCAACG	10405	0.0	32.36146	3
CAACGCA	10795	0.0	31.191961	5
AACGCAG	11290	0.0	30.086954	6
TATCAAC	11405	0.0	29.901937	2
ACGCAGA	12870	0.0	26.227924	7
CGCAGAG	12960	0.0	26.045786	8
GCAGAGT	15705	0.0	21.384954	9
TGGTATC	2510	0.0	20.015682	2
GAGTACT	9235	0.0	19.474176	12-13
CAGAGTA	15385	0.0	18.56497	10-11
TACATGG	11015	0.0	18.553202	2
GTGGTAT	2760	0.0	18.356928	1
GTACATG	11405	0.0	18.292072	1
AGAGTAC	14225	0.0	17.774746	10-11
ACATGGG	11130	0.0	17.670162	3
GTACTTT	10450	0.0	17.210146	14-15
AGTACTT	10050	0.0	16.073668	12-13
>>END_MODULE