FastQCFastQC Report
Fri 27 May 2016
SRR2140065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4950636
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC55000.049.5188671
GGTATCA56650.046.5551641
GTATCAA99700.037.777451
TATACAC77450.037.6662943
TTATACA82900.033.8929562
ATCAACG118400.031.1171673
TCAACGC119350.030.9426294
CAACGCA121450.030.407295
TATCAAC125900.029.67632
AACGCAG126400.029.28356
TACACAT104900.028.0568985
ACACATC100200.027.3505636
ACATCTC100900.027.1608188
ACGCAGA144850.025.4940727
CGCAGAG146800.025.1260728
CACATCT115800.023.666037
CATCTCC116200.023.3991539
ATACACA135150.023.116434
GTGGTAT28850.023.0033591
TGGTATC27900.021.1608312
Produced by FastQC (version 0.11.5)