##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140065_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4950636
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.145774805499737	31.0	18.0	33.0	18.0	33.0
2	30.211972562717193	32.0	27.0	33.0	25.0	33.0
3	31.304798211785315	33.0	31.0	33.0	28.0	33.0
4	32.43584299067837	33.0	33.0	33.0	31.0	34.0
5	32.912732222688156	33.0	33.0	34.0	32.0	34.0
6	37.072749844666426	38.0	37.0	38.0	36.0	38.0
7	37.18030935823195	38.0	38.0	38.0	36.0	38.0
8	37.41311479979542	38.0	38.0	38.0	37.0	38.0
9	37.46082685133789	38.0	38.0	38.0	37.0	38.0
10-11	37.48414153252229	38.0	38.0	38.0	37.0	38.0
12-13	37.50519064621192	38.0	38.0	38.0	37.0	38.0
14-15	37.512819968989845	38.0	38.0	38.0	37.0	38.0
16-17	37.45075370114062	38.0	38.0	38.0	37.0	38.0
18-19	37.48423808577322	38.0	38.0	38.0	37.0	38.0
20-21	37.507967748285516	38.0	38.0	38.0	37.0	38.0
22-23	37.51561857935988	38.0	38.0	38.0	37.0	38.0
24-25	37.51062778146836	38.0	38.0	38.0	37.0	38.0
26-27	37.4779325791136	38.0	38.0	38.0	37.0	38.0
28-29	37.4484437342078	38.0	38.0	38.0	37.0	38.0
30-31	37.35669955438076	38.0	38.0	38.0	37.0	38.0
32-33	37.415523848344726	38.0	38.0	38.0	37.0	38.0
34-35	37.41468229651943	38.0	38.0	38.0	37.0	38.0
36-37	37.3937083028568	38.0	38.0	38.0	37.0	38.0
38-39	37.352496940408585	38.0	38.0	38.0	37.0	38.0
40-41	37.37333705623324	38.0	38.0	38.0	37.0	38.0
42-43	37.35403257581082	38.0	38.0	38.0	37.0	38.0
44-45	37.322445841932634	38.0	38.0	38.0	37.0	38.0
46-47	37.29662310734853	38.0	38.0	38.0	37.0	38.0
48-49	37.270991305154766	38.0	38.0	38.0	37.0	38.0
50-51	37.229874060643326	38.0	38.0	38.0	37.0	38.0
52-53	37.20789442979432	38.0	38.0	38.0	37.0	38.0
54-55	37.19336197350617	38.0	38.0	38.0	37.0	38.0
56-57	37.18372183353216	38.0	38.0	38.0	37.0	38.0
58-59	37.17132659937908	38.0	38.0	38.0	37.0	38.0
60-61	37.16698956373008	38.0	38.0	38.0	36.0	38.0
62-63	37.17108664179744	38.0	38.0	38.0	36.5	38.0
64-65	37.17397321523031	38.0	38.0	38.0	36.0	38.0
66-67	37.14971103227197	38.0	38.0	38.0	36.0	38.0
68-69	37.147503117797754	38.0	38.0	38.0	36.0	38.0
70-71	37.124776227954456	38.0	38.0	38.0	36.0	38.0
72-73	37.11244327214605	38.0	38.0	38.0	36.0	38.0
74-75	37.103724324127434	38.0	38.0	38.0	36.0	38.0
76-77	37.10920489128425	38.0	38.0	38.0	36.0	38.0
78-79	37.095404801885266	38.0	38.0	38.0	36.0	38.0
80-81	37.09576340001544	38.0	38.0	38.0	36.0	38.0
82-83	37.04800805065658	38.0	38.0	38.0	36.0	38.0
84-85	37.037373585199816	38.0	38.0	38.0	36.0	38.0
86-87	37.032073292035676	38.0	38.0	38.0	36.0	38.0
88-89	37.03860522559154	38.0	38.0	38.0	36.0	38.0
90-91	37.02941257498731	38.0	38.0	38.0	36.0	38.0
92-93	37.01429409001451	38.0	38.0	38.0	36.0	38.0
94-95	36.986273542564746	38.0	38.0	38.0	36.0	38.0
96-97	36.95798418517211	38.0	38.0	38.0	36.0	38.0
98-99	36.9619683739657	38.0	38.0	38.0	35.5	38.0
100	35.41374334725821	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	6.0
19	12.0
20	41.0
21	119.0
22	295.0
23	928.0
24	1901.0
25	3671.0
26	6634.0
27	10917.0
28	17041.0
29	24950.0
30	34645.0
31	45262.0
32	59337.0
33	78788.0
34	117321.0
35	217672.0
36	703040.0
37	3628053.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.40984552287827	21.756517748426667	14.343672206964921	24.489964521730137
2	13.759968938948752	20.76623993409627	42.255695721287665	23.21809540566731
3	17.250834034253376	26.254384285170634	31.545542835304392	24.949238845271598
4	12.146885180201753	18.82698623708938	39.22786057672399	29.798268005984873
5	13.046224589848018	37.58438067242745	36.05448923963364	13.314905498090893
6	28.956521949907042	38.757828287113014	19.042805813232885	13.242843949747062
7	25.52987131350396	32.88997615659887	23.518614578005735	18.061537951891435
8	23.445937047280392	37.465044895241746	21.76595087984655	17.32306717763132
9	24.737442029999873	17.097673200998422	21.669927873695038	36.49495689530667
10-11	23.44623013818997	27.16101290496883	29.571340919936674	19.821416036904523
12-13	24.37644375389344	24.5149411105967	29.50545142078715	21.603163714722715
14-15	21.556705037494172	26.04035117912123	26.839622222276088	25.56332156110851
16-17	21.437164841042648	29.298811304244545	27.542410712482194	21.721613142230613
18-19	21.380788246197056	28.266913584436427	29.254170575255383	21.09812759411114
20-21	22.037201290111202	27.43473947805318	29.10435159024769	21.423707641587928
22-23	21.714625252781737	27.7553587909867	28.940945782951218	21.58907017328035
24-25	21.644534057673265	27.887259043239393	29.133642494932488	21.334564404154854
26-27	21.78629089648801	27.938773669271587	29.054404941938543	21.220530492301858
28-29	21.472139161770524	27.67616127369606	29.325199073109015	21.5265004914244
30-31	21.968936753523145	27.776429976735685	29.00143448953763	21.25319878020354
32-33	21.7097206609735	27.571477951205832	29.135987622753323	21.582813765067343
34-35	21.424191093388092	27.902582796204918	29.49594839667764	21.177277713729346
36-37	21.785988759655243	27.716863309182443	29.063630003246523	21.43351792791579
38-39	21.412062142917566	27.58713922550967	29.352926127501256	21.647872504071504
40-41	21.61885615162784	27.47908605316069	29.37596831455449	21.526089480656978
42-43	21.808417841718633	27.456318568425004	29.19470282628509	21.54056076357127
44-45	21.596883190940193	27.754438114007883	29.06348791492427	21.585190780127654
46-47	21.93293726980737	27.465014604348205	28.66826045367601	21.93378767216842
48-49	21.743583398633508	27.533461712947837	28.749588899710567	21.973365988708093
50-51	21.525206674308375	27.70284298416769	28.905571087167658	21.866379254356275
52-53	21.678557640245643	27.67664238324235	29.090807977215793	21.55399199929622
54-55	21.697006940841238	27.63943560867145	28.765638516519076	21.89791893396823
56-57	21.96871009231268	27.94925615709295	28.343665480419318	21.73836827017506
58-59	21.52534357593326	28.317509220932525	28.842153672035302	21.314993531098914
60-61	21.890563378605812	28.7267164915129	28.149649934843413	21.233070195037875
62-63	21.547964507373173	29.00917545017479	28.076449174802086	21.36641086764995
64-65	21.509719547538573	29.007942161082763	28.09953498337118	21.382803308007485
66-67	21.555150226310918	28.894210872401622	28.046678855127166	21.503960046160298
68-69	21.63945685418433	28.672275771299393	28.133407699802692	21.554859674713583
70-71	21.725143519951047	28.30173675994959	28.248759848179716	21.724359871919642
72-73	21.827274064836157	27.96870292652509	28.359618577864342	21.844404430774407
74-75	21.774615798970363	27.99290026520983	28.349547141709174	21.882936794110634
76-77	21.858564124270014	27.916266121557364	28.294332435566293	21.93083731860633
78-79	21.83293766378983	27.978661379899027	28.252253999494787	21.93614695681635
80-81	21.864619677983804	27.98470507008667	28.23904088587121	21.91163436605832
82-83	21.94592200417439	27.955443625651803	28.31030565325871	21.788328716915096
84-85	22.0132904796277	27.930067253325447	28.21234499231257	21.84429727473428
86-87	21.858694989524647	27.930955351252706	28.29108744925265	21.91926220997
88-89	21.936997171558765	27.916354951372412	28.215839170910662	21.93080870615816
90-91	21.9578186656108	27.975300239399115	28.228642117563602	21.83823897742648
92-93	21.99681319491665	27.911035385610166	28.21877565908547	21.873375760387713
94-95	21.906941423161825	28.05182195398448	28.192449576284538	21.848787046569154
96-97	22.015348352921578	28.14366546784975	28.225647897986928	21.615338281241744
98-99	23.070374869812444	30.037776552703832	28.46560614285469	18.42624243462903
100	21.920511585718113	30.958241091772155	22.530262798321893	24.590984524187835
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	621.0
1	631.0
2	950.0
3	1430.5
4	1863.0
5	2436.0
6	2973.5
7	3826.0
8	4966.5
9	5759.5
10	6021.5
11	6283.0
12	7000.5
13	8320.0
14	10736.5
15	13381.0
16	15289.5
17	16358.0
18	16224.0
19	15358.0
20	14656.5
21	14791.0
22	16382.5
23	19941.0
24	25532.5
25	33034.5
26	42991.5
27	54846.5
28	66824.5
29	78993.5
30	90546.5
31	102015.0
32	114543.5
33	126624.0
34	137403.5
35	147527.5
36	158955.5
37	169799.5
38	177508.0
39	183857.0
40	190593.5
41	195574.5
42	201676.0
43	220757.0
44	229934.0
45	223630.0
46	216772.5
47	208048.0
48	202126.5
49	194917.5
50	187831.0
51	179667.5
52	168571.5
53	156920.5
54	145470.0
55	133727.5
56	121364.0
57	108832.5
58	96542.0
59	83422.5
60	69040.0
61	55369.0
62	43456.5
63	33247.0
64	24744.0
65	18137.0
66	13249.5
67	9953.5
68	7569.0
69	5409.5
70	3872.0
71	2830.0
72	1939.5
73	1321.0
74	879.5
75	558.5
76	348.0
77	202.0
78	127.0
79	82.0
80	54.5
81	31.0
82	19.0
83	16.5
84	12.5
85	8.0
86	6.0
87	5.0
88	3.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007776778579560282
3	0.0
4	0.003171309706470037
5	0.0031107114318241133
6	0.0
7	0.0
8	0.0
9	3.0299137322962143E-4
10-11	4.746864847264069E-4
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.888061132084466E-4
24-25	0.001393874346469892
26-27	0.0
28-29	0.001909683103750355
30-31	0.0028314818307350275
32-33	6.078364709176589E-4
34-35	1.0154775018928501E-5
36-37	0.001192211496281472
38-39	0.002406692777064361
40-41	0.001493058769779296
42-43	0.0
44-45	0.0
46-47	0.0013228306181534985
48-49	4.758694531466867E-4
50-51	0.001671551607665331
52-53	0.00233588605676396
54-55	0.0016932013973548958
56-57	0.0019223745170034027
58-59	0.001750242764140895
60-61	6.940879898649933E-4
62-63	7.435209913731589E-4
64-65	0.003320957826854652
66-67	0.0022339495799836313
68-69	9.120981381112682E-5
70-71	0.0027196456716551036
72-73	0.010053007723301996
74-75	0.011807948833320502
76-77	0.004961191730476731
78-79	0.004082233175482994
80-81	3.019171983629808E-4
82-83	8.527108836240895E-5
84-85	4.913662044220992E-5
86-87	0.0019205685081036218
88-89	0.004312986828388254
90-91	4.542417663948156E-4
92-93	8.660413593334233E-4
94-95	0.0018904316125433096
96-97	0.0019308028943779063
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	40.0
20-21	87.0
22-23	219.0
24-25	330.0
26-27	988.0
28-29	2541.0
30-31	8520.0
32-33	10765.0
34-35	14971.0
36-37	23171.0
38-39	26779.0
40-41	26035.0
42-43	29723.0
44-45	35006.0
46-47	34909.0
48-49	32674.0
50-51	30306.0
52-53	29552.0
54-55	32089.0
56-57	32859.0
58-59	32598.0
60-61	32965.0
62-63	33164.0
64-65	37118.0
66-67	44521.0
68-69	47148.0
70-71	59277.0
72-73	36773.0
74-75	33918.0
76-77	35823.0
78-79	36355.0
80-81	36216.0
82-83	34344.0
84-85	34699.0
86-87	35567.0
88-89	36547.0
90-91	37071.0
92-93	36879.0
94-95	44403.0
96-97	149018.0
98-99	334441.0
100-101	3370227.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.5703149416859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.27012928201879	78.1471352177107
2	7.057812592460709	12.219941178574405
3	1.6011449681561818	4.158348724817289
4	0.5117793058174543	1.7721958274101761
5	0.22130323743512598	0.9579145481186774
6	0.11951513131146978	0.620787753475853
7	0.06370852059016614	0.38606866843716947
8	0.03988804842982626	0.2762496731983423
9	0.029243285759836864	0.22784404123429164
>10	0.08344752703962406	1.0651902042312238
>50	0.0016073468087161723	0.09299225333374711
>100	4.20754172050797E-4	0.07533190945809622
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.019942488197476E-5	0.0
2	0.0	2.019942488197476E-5	0.0	4.039884976394952E-5	0.0
3	0.0	2.019942488197476E-5	0.0	4.039884976394952E-5	0.0
4	0.0	4.039884976394952E-5	0.0	4.039884976394952E-5	0.0
5	0.0	4.039884976394952E-5	0.0	4.039884976394952E-5	0.0
6	0.0	4.039884976394952E-5	0.0	4.039884976394952E-5	0.0
7	0.0	4.039884976394952E-5	0.0	4.039884976394952E-5	0.0
8	0.0	4.039884976394952E-5	0.0	6.059827464592428E-5	0.0
9	0.0	8.079769952789905E-5	0.0	6.059827464592428E-5	0.0
10-11	0.0	8.079769952789905E-5	0.0	2.019942488197476E-4	0.0
12-13	0.0	8.079769952789905E-5	0.0	2.2219367370172237E-4	0.0
14-15	0.0	1.6159539905579806E-4	0.0	2.625925234656719E-4	2.019942488197476E-5
16-17	4.039884976394952E-5	1.8179482393777284E-4	0.0	2.827919483476466E-4	4.039884976394952E-5
18-19	4.039884976394952E-5	1.9189453637876022E-4	0.0	2.827919483476466E-4	4.039884976394952E-5
20-21	4.039884976394952E-5	2.019942488197476E-4	0.0	2.827919483476466E-4	5.0498562204936905E-5
22-23	4.039884976394952E-5	2.4239309858369712E-4	0.0	2.827919483476466E-4	6.059827464592428E-5
24-25	6.059827464592428E-5	2.625925234656719E-4	0.0	2.827919483476466E-4	8.079769952789905E-5
26-27	6.059827464592428E-5	2.9289166078863403E-4	0.0	3.0299137322962143E-4	8.079769952789905E-5
28-29	6.059827464592428E-5	3.231907981115962E-4	0.0	4.544870598444321E-4	8.079769952789905E-5
30-31	6.059827464592428E-5	3.635896478755457E-4	0.0	0.0010200709565397256	8.079769952789905E-5
32-33	6.059827464592428E-5	3.8378907275752043E-4	0.0	0.002514828397805858	8.079769952789905E-5
34-35	7.069798708691166E-5	4.0398849763949524E-4	0.0	0.004413574336711485	9.089741196888642E-5
36-37	8.079769952789905E-5	4.2418792252147E-4	0.0	0.006918303022076355	1.009971244098738E-4
38-39	8.079769952789905E-5	4.645867722854195E-4	0.0	0.010998586848235258	1.009971244098738E-4
40-41	8.079769952789905E-5	6.059827464592429E-4	0.0	0.015088970386835147	1.009971244098738E-4
42-43	1.009971244098738E-4	6.160824589002302E-4	0.0	0.019209653062757997	1.009971244098738E-4
44-45	1.009971244098738E-4	7.271792957510914E-4	0.0	0.02381512193584824	1.009971244098738E-4
46-47	1.009971244098738E-4	8.281764201609652E-4	0.0	0.028774080744373046	1.009971244098738E-4
48-49	1.009971244098738E-4	8.281764201609652E-4	0.0	0.033632042428487975	1.009971244098738E-4
50-51	1.009971244098738E-4	9.190738321298516E-4	0.0	0.03884349404803747	1.009971244098738E-4
52-53	1.009971244098738E-4	9.29173544570839E-4	0.0	0.043974147968059055	1.1109683685086119E-4
54-55	1.009971244098738E-4	9.897718192167634E-4	0.0	0.048347323455006586	1.2119654929184856E-4
56-57	1.009971244098738E-4	0.001009971244098738	0.0	0.05332648168841336	1.2119654929184856E-4
58-59	1.009971244098738E-4	0.001009971244098738	0.0	0.05803294768591349	1.3129626173283594E-4
60-61	1.009971244098738E-4	0.0010402703814217	0.0	0.062274826911128185	1.413959741738233E-4
62-63	1.009971244098738E-4	0.001060469806303675	0.0	0.06662780297319375	1.413959741738233E-4
64-65	1.009971244098738E-4	0.0011715666431545361	0.0	0.0713039698333709	1.413959741738233E-4
66-67	1.3129626173283594E-4	0.0011816663555955236	0.0	0.07592963813134312	1.413959741738233E-4
68-69	1.413959741738233E-4	0.0012018657804774983	0.0	0.0805856055666383	1.413959741738233E-4
70-71	1.413959741738233E-4	0.001222065205359473	0.0	0.08469618853012018	1.615953990557981E-4
72-73	1.6159539905579806E-4	0.0012321649178004604	0.0	0.08806949248540996	1.615953990557981E-4
74-75	1.8179482393777284E-4	0.0012422646302414479	0.0	0.09058432088321582	1.615953990557981E-4
76-77	1.8179482393777284E-4	0.0013028629048873722	0.0	0.0931496478432266	1.615953990557981E-4
78-79	1.8179482393777284E-4	0.0013331620422103341	0.0	0.09470500355913866	1.615953990557981E-4
80-81	1.8179482393777284E-4	0.001353361467092309	0.0	0.09528068716827495	1.8179482393777284E-4
82-83	1.8179482393777284E-4	0.001353361467092309	0.0	0.0957957725027653	2.019942488197476E-4
84-85	1.8179482393777284E-4	0.001353361467092309	0.0	0.09597756732670307	2.019942488197476E-4
86-87	1.8179482393777284E-4	0.001353361467092309	0.0	0.09606846473867196	2.019942488197476E-4
88	1.8179482393777284E-4	0.0013937603168562584	0.0	0.09608866416355394	2.019942488197476E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	5500	0.0	49.518867	1
GGTATCA	5665	0.0	46.555164	1
GTATCAA	9970	0.0	37.77745	1
TATACAC	7745	0.0	37.666294	3
TTATACA	8290	0.0	33.892956	2
ATCAACG	11840	0.0	31.117167	3
TCAACGC	11935	0.0	30.942629	4
CAACGCA	12145	0.0	30.40729	5
TATCAAC	12590	0.0	29.6763	2
AACGCAG	12640	0.0	29.2835	6
TACACAT	10490	0.0	28.056898	5
ACACATC	10020	0.0	27.350563	6
ACATCTC	10090	0.0	27.160818	8
ACGCAGA	14485	0.0	25.494072	7
CGCAGAG	14680	0.0	25.126072	8
CACATCT	11580	0.0	23.66603	7
CATCTCC	11620	0.0	23.399153	9
ATACACA	13515	0.0	23.11643	4
GTGGTAT	2885	0.0	23.003359	1
TGGTATC	2790	0.0	21.160831	2
>>END_MODULE