FastQCFastQC Report
Fri 27 May 2016
SRR2140064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4261578
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49000.050.672151
CTTATAC50200.049.8857841
TATACAC70150.039.2237363
GTATCAA89950.038.274961
TTATACA73350.035.3045732
ATCAACG105400.032.3792153
TCAACGC105650.032.3433534
CAACGCA108500.031.4537265
TATCAAC111250.030.8701152
AACGCAG112150.030.5054026
ACATCTC91450.028.5942298
ACACATC92550.027.885646
TACACAT98550.027.1840935
ACGCAGA130400.026.2033377
CGCAGAG130750.026.1984448
CACATCT101450.025.355197
CATCTCC102400.024.953339
ATACACA123800.023.0874614
TATGCCG66100.022.4788942-43
CGTATGC67150.021.93964640-41