##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140064_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4261578
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.389010361889422	31.0	18.0	33.0	18.0	33.0
2	30.273088278567236	32.0	28.0	33.0	25.0	33.0
3	31.331362936452177	33.0	31.0	33.0	28.0	33.0
4	32.431797798843526	33.0	33.0	33.0	31.0	34.0
5	32.909842081970574	33.0	33.0	34.0	32.0	34.0
6	37.08701072701239	38.0	37.0	38.0	36.0	38.0
7	37.19504441781894	38.0	38.0	38.0	36.0	38.0
8	37.435697293350024	38.0	38.0	38.0	37.0	38.0
9	37.48656577446195	38.0	38.0	38.0	37.0	38.0
10-11	37.5072842735719	38.0	38.0	38.0	37.0	38.0
12-13	37.52608317857845	38.0	38.0	38.0	37.0	38.0
14-15	37.533216334418846	38.0	38.0	38.0	37.5	38.0
16-17	37.470114474028165	38.0	38.0	38.0	37.0	38.0
18-19	37.502819260846564	38.0	38.0	38.0	37.0	38.0
20-21	37.52796746229938	38.0	38.0	38.0	37.0	38.0
22-23	37.53469470485848	38.0	38.0	38.0	37.5	38.0
24-25	37.528077527203635	38.0	38.0	38.0	38.0	38.0
26-27	37.49628053292638	38.0	38.0	38.0	37.0	38.0
28-29	37.47226047247271	38.0	38.0	38.0	37.0	38.0
30-31	37.374720892307295	38.0	38.0	38.0	37.0	38.0
32-33	37.43774529500852	38.0	38.0	38.0	37.0	38.0
34-35	37.44392428048047	38.0	38.0	38.0	37.0	38.0
36-37	37.42221965453351	38.0	38.0	38.0	37.0	38.0
38-39	37.387570241453936	38.0	38.0	38.0	37.0	38.0
40-41	37.39474087665516	38.0	38.0	38.0	37.0	38.0
42-43	37.37290862889541	38.0	38.0	38.0	37.0	38.0
44-45	37.339539441825494	38.0	38.0	38.0	37.0	38.0
46-47	37.31527723963322	38.0	38.0	38.0	37.0	38.0
48-49	37.2879148424383	38.0	38.0	38.0	37.0	38.0
50-51	37.24756453837193	38.0	38.0	38.0	37.0	38.0
52-53	37.22628738627017	38.0	38.0	38.0	37.0	38.0
54-55	37.209922258634634	38.0	38.0	38.0	37.0	38.0
56-57	37.199521450635615	38.0	38.0	38.0	37.0	38.0
58-59	37.188800167826564	38.0	38.0	38.0	37.0	38.0
60-61	37.18404030868493	38.0	38.0	38.0	37.0	38.0
62-63	37.192534778863326	38.0	38.0	38.0	36.5	38.0
64-65	37.19470194808717	38.0	38.0	38.0	37.0	38.0
66-67	37.171550880564965	38.0	38.0	38.0	36.0	38.0
68-69	37.16812235685293	38.0	38.0	38.0	36.0	38.0
70-71	37.15130678401658	38.0	38.0	38.0	36.0	38.0
72-73	37.14240553058776	38.0	38.0	38.0	36.0	38.0
74-75	37.13431832182735	38.0	38.0	38.0	36.0	38.0
76-77	37.13737672888941	38.0	38.0	38.0	36.0	38.0
78-79	37.12661996767771	38.0	38.0	38.0	36.0	38.0
80-81	37.12987625289953	38.0	38.0	38.0	36.0	38.0
82-83	37.08451488657404	38.0	38.0	38.0	36.0	38.0
84-85	37.07561082874152	38.0	38.0	38.0	36.0	38.0
86-87	37.06988683508349	38.0	38.0	38.0	36.0	38.0
88-89	37.07723993264869	38.0	38.0	38.0	36.0	38.0
90-91	37.06844513927595	38.0	38.0	38.0	36.0	38.0
92-93	37.054909939494436	38.0	38.0	38.0	36.0	38.0
94-95	37.029632863891386	38.0	38.0	38.0	36.0	38.0
96-97	37.00239943230265	38.0	38.0	38.0	36.0	38.0
98-99	37.00201924431038	38.0	38.0	38.0	36.0	38.0
100	35.46558851348539	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	8.0
20	51.0
21	97.0
22	240.0
23	643.0
24	1459.0
25	2948.0
26	5263.0
27	8862.0
28	13886.0
29	20674.0
30	28170.0
31	37229.0
32	48759.0
33	65638.0
34	98049.0
35	182421.0
36	608461.0
37	3138718.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.506140683099076	21.675820552856244	14.332789403361854	24.485249360682825
2	13.753515967290534	20.79988285193583	42.23642976255458	23.21017141821906
3	17.123539684126396	26.30408266609223	31.747136858694127	24.825240791087243
4	12.19766717682214	18.98235950895541	39.13272630933256	29.687247004889887
5	12.94083772778728	37.6404926794561	36.1659414018574	13.25272819089921
6	28.82556649203652	38.81644311097908	19.041420807034388	13.316569589950014
7	25.453435323722807	32.993506161332725	23.451805880356993	18.10125263458747
8	23.481114272694292	37.3536750940614	21.84200312654139	17.323207506702918
9	24.787191205498814	16.98589159117809	21.625306342354417	36.60161086096868
10-11	23.408597704688148	27.11591728467255	29.689237921547907	19.786247089091404
12-13	24.359729316134533	24.529994944359206	29.51178982707456	21.598485912431702
14-15	21.565955146192326	26.049036295944834	26.84807130128793	25.53693725657491
16-17	21.372763797823247	29.371103849325298	27.547377990030924	21.70875436282053
18-19	21.425866193227016	28.260153867886494	29.223236087665178	21.09074385122131
20-21	22.069190383785735	27.425914838362687	29.082713573746904	21.422181204104675
22-23	21.741315889824296	27.787134434308175	28.883752309993838	21.58779736587369
24-25	21.874432368878676	27.78273603480767	29.039902561057744	21.302929035255907
26-27	21.861683470437885	27.888381042102544	28.958227205950433	21.291708281509138
28-29	21.557561891256398	27.633980820724606	29.113196228944528	21.69526105907447
30-31	21.772596265085202	27.652327350467626	29.240569204409024	21.334507180038152
32-33	21.197505922227595	27.51783439903007	29.523047961358163	21.761611717384174
34-35	21.29477480780424	27.62282480515848	29.21733380831072	21.86506657872656
36-37	21.40438656849887	27.7521798866249	29.46964978407821	21.37378376079802
38-39	21.33175258740741	27.576888333068815	29.445788250849343	21.64557082867443
40-41	21.574179397856817	27.481561310503977	29.426990763602408	21.5172685280368
42-43	21.870838455002435	27.436433892093838	29.247987894126794	21.44473975877694
44-45	21.532410976220138	27.81980790518744	29.08540023405999	21.562380884532434
46-47	21.959929499345368	27.447154295785104	28.63533279672127	21.957583408148256
48-49	21.745119603967115	27.49872449437768	28.77011778004672	21.986038121608484
50-51	21.470245244764165	27.641160355687422	28.989493504687513	21.899100894860897
52-53	21.656880216388846	27.606454291075394	29.24080175744887	21.495863735086893
54-55	21.675149593625314	27.62263332419411	28.79909780106113	21.90311928111945
56-57	21.986670990546006	27.911909856567352	28.359186138279757	21.74223301460689
58-59	21.455569975423273	28.361633629231765	28.952739473707588	21.23005692163737
60-61	21.859900873675976	28.777821847765768	28.167017005766187	21.195260272792073
62-63	21.4442466020459	29.21460783377401	28.076801808578978	21.264343755601114
64-65	21.43941973769503	29.169490736018993	28.087475803822386	21.30361372246359
66-67	21.478245303156307	29.060441983898365	28.015351191427495	21.445961521517834
68-69	21.56661327296554	28.818638735772396	28.129816830291652	21.484931160970408
70-71	21.621319005818368	28.421918334202452	28.270720776630835	21.68604188334835
72-73	21.826905381164924	28.03249009421307	28.341650979270465	21.798953545351544
74-75	21.785447345961373	28.058820311945905	28.319532969442605	21.836199372650114
76-77	21.833396852283155	28.00928788199591	28.30671183569765	21.85060343002329
78-79	21.837541585234554	28.038707254197565	28.242750618542072	21.88100054202581
80-81	21.836731376245144	27.98445543006159	28.266755707614426	21.91205748607884
82-83	21.889356077935847	27.951417180453586	28.303885899363124	21.855340842247443
84-85	21.994027992010157	27.91144917277678	28.235584003367848	21.85893883184522
86-87	21.89181566406761	27.91294432122946	28.31953897667833	21.875701038024598
88-89	21.9564833080612	27.948811557030123	28.171292038058986	21.923413096849693
90-91	21.97461400739519	27.967774254964162	28.23627181872875	21.821339918911896
92-93	21.95430692390804	27.943570696022295	28.253787339995952	21.848335040073717
94-95	21.916321089431367	28.03841071906408	28.198332140200293	21.846936051304258
96-97	22.005611582947708	28.16072964651844	28.25375563703257	21.57990313350129
98-99	23.045199670872744	30.0250185294327	28.48937808915246	18.440403710542093
100	21.870236732928362	31.01019590378152	22.514368281181135	24.605199082108985
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	585.0
1	570.0
2	844.5
3	1295.0
4	1648.5
5	2070.0
6	2530.5
7	3352.5
8	4360.0
9	5128.0
10	5507.0
11	5648.5
12	6172.0
13	7323.0
14	9509.0
15	11942.0
16	13491.0
17	14291.0
18	14340.0
19	13773.5
20	13224.0
21	13223.5
22	14529.5
23	17721.5
24	22928.5
25	30278.0
26	39388.5
27	49399.5
28	59753.0
29	70478.5
30	80038.5
31	89805.0
32	100908.5
33	111084.5
34	121133.5
35	130415.5
36	139502.0
37	148166.5
38	154739.5
39	160349.0
40	167146.5
41	172139.0
42	174293.5
43	177353.0
44	190345.0
45	207248.0
46	201287.0
47	188704.0
48	180263.5
49	168774.0
50	161678.0
51	153965.5
52	144685.5
53	135504.5
54	126358.5
55	115787.0
56	105139.5
57	94285.0
58	83004.5
59	72470.5
60	60811.0
61	49217.0
62	38601.0
63	29522.0
64	22403.0
65	16520.0
66	12038.0
67	9092.5
68	6894.5
69	5013.5
70	3755.5
71	2772.5
72	1955.0
73	1374.0
74	896.0
75	560.5
76	371.0
77	241.0
78	150.5
79	94.0
80	59.0
81	44.0
82	34.0
83	22.5
84	14.0
85	9.0
86	6.0
87	4.5
88	2.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007133507822689154
3	0.0
4	0.0030974441861676594
5	0.003425960993791502
6	0.0
7	0.0
8	0.0
9	1.4079291755307542E-4
10-11	5.162406976946098E-4
12-13	3.5198229388268855E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0010559860328247393
24-25	0.0014197910184958874
26-27	0.0
28-29	0.0019486459023967877
30-31	0.0029964155819250573
32-33	6.712696134382052E-4
34-35	5.9057166510381716E-5
36-37	0.0011031122949337966
38-39	0.002374642446268345
40-41	0.001501108238190091
42-43	0.0
44-45	0.0
46-47	0.0013511337424648192
48-49	5.82164751138194E-4
50-51	0.0016602198955010163
52-53	0.0022383858270439514
54-55	0.0016217221574378008
56-57	0.0020690841658736244
58-59	0.0019313865900738834
60-61	7.787175456484224E-4
62-63	8.896287343801472E-4
64-65	0.003417133586966445
66-67	0.002412561313712945
68-69	1.6185332851370089E-4
70-71	0.0028537332018886523
72-73	0.009782878300162539
74-75	0.012588498895638968
76-77	0.004940118703995145
78-79	0.004516677725640467
80-81	3.740396352538111E-4
82-83	7.262000637603656E-5
84-85	8.794859463276111E-5
86-87	0.0021456112409904676
88-89	0.004647247051538418
90-91	5.886414833765381E-4
92-93	0.001082423005422177
94-95	0.0020796592686254285
96-97	0.002329895720747768
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	32.0
20-21	86.0
22-23	212.0
24-25	302.0
26-27	1056.0
28-29	2670.0
30-31	8986.0
32-33	11579.0
34-35	15698.0
36-37	23973.0
38-39	26965.0
40-41	26280.0
42-43	29572.0
44-45	34651.0
46-47	34726.0
48-49	31859.0
50-51	29617.0
52-53	29131.0
54-55	31503.0
56-57	31663.0
58-59	30857.0
60-61	30682.0
62-63	31416.0
64-65	35956.0
66-67	42846.0
68-69	44679.0
70-71	56450.0
72-73	35602.0
74-75	31967.0
76-77	32951.0
78-79	33680.0
80-81	33300.0
82-83	31727.0
84-85	31733.0
86-87	32913.0
88-89	33152.0
90-91	33336.0
92-93	33032.0
94-95	39093.0
96-97	125040.0
98-99	279378.0
100-101	2811227.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.22117103088013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.41747022726038	80.64956276129529
2	6.291836890392939	11.10146436811513
3	1.3924599547010796	3.685333434520066
4	0.44622485554630714	1.5746591719752216
5	0.18923828008833954	0.83474113366315
6	0.09112600729452641	0.4823545844934986
7	0.05475176791417242	0.33811855569794813
8	0.03292876899545634	0.23240116491075974
9	0.021203318538772702	0.16835234321081682
>10	0.06137909503609358	0.7836565556918217
>50	8.559055463399335E-4	0.0568063954071745
>100	4.878337732101085E-4	0.07579404118908363
>500	3.709491235707794E-5	0.016755489830037922
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.6425840381192132E-4	0.0	4.6930972517691804E-5	0.0
5	0.0	1.6425840381192132E-4	0.0	4.6930972517691804E-5	0.0
6	0.0	1.6425840381192132E-4	0.0	4.6930972517691804E-5	0.0
7	0.0	1.6425840381192132E-4	0.0	4.6930972517691804E-5	0.0
8	0.0	1.6425840381192132E-4	0.0	4.6930972517691804E-5	0.0
9	0.0	2.581203488473049E-4	0.0	4.6930972517691804E-5	0.0
10-11	0.0	2.581203488473049E-4	0.0	5.866371564711476E-5	0.0
12-13	0.0	2.581203488473049E-4	0.0	7.039645877653771E-5	0.0
14-15	0.0	3.4024955075326557E-4	0.0	8.212920190596066E-5	1.1732743129422951E-5
16-17	0.0	3.519822938826885E-4	0.0	9.386194503538361E-5	2.3465486258845902E-5
18-19	0.0	3.8718052327095737E-4	0.0	1.0559468816480656E-4	3.5198229388268855E-5
20-21	0.0	4.341114957886492E-4	0.0	1.1732743129422951E-4	4.6930972517691804E-5
22-23	0.0	5.162406976946098E-4	0.0	1.2906017442365246E-4	4.6930972517691804E-5
24-25	0.0	5.749044133417246E-4	0.0	2.2292211945903605E-4	4.6930972517691804E-5
26-27	0.0	6.218353858594163E-4	0.0	2.933185782355738E-4	4.6930972517691804E-5
28-29	0.0	6.335681289888393E-4	0.0	4.8104246830634096E-4	5.866371564711476E-5
30-31	0.0	7.27430074024223E-4	0.0	0.0012436707717188328	7.039645877653771E-5
32-33	0.0	7.626283034124917E-4	0.0	0.0029918494980028525	7.039645877653771E-5
34-35	0.0	9.034212209655672E-4	0.0	0.004740028224286872	9.386194503538361E-5
36-37	0.0	0.0010794123679069116	0.0	0.007415093657795305	9.386194503538361E-5
38-39	0.0	0.0011028778541657574	0.0	0.011509821009963915	1.0559468816480656E-4
40-41	0.0	0.0012906017442365246	0.0	0.016120789059827133	1.1732743129422951E-4
42-43	0.0	0.0013375327167542165	0.0	0.0206026969352667	1.1732743129422951E-4
44-45	0.0	0.0015252566068249835	0.0	0.025706440196565684	1.2906017442365246E-4
46-47	0.0	0.0016425840381192132	0.0	0.030892312659770632	1.4079291755307542E-4
48-49	0.0	0.001654316781248636	0.0	0.03646536564624653	1.4079291755307542E-4
50-51	0.0	0.001971100845743056	0.0	0.041545643421286665	1.4079291755307542E-4
52-53	0.0	0.001994566332001902	0.0	0.04649686102190315	1.6425840381192132E-4
54-55	0.0	0.0020062990751313244	0.0	0.05143634587939022	1.8772389007076722E-4
56-57	0.0	0.0020414973045195933	0.0	0.05723232098532516	1.8772389007076722E-4
58-59	0.0	0.0020414973045195933	0.0	0.06251205539356548	1.8772389007076722E-4
60-61	0.0	0.0020884282770372853	0.0	0.06789738448997062	1.8772389007076722E-4
62-63	0.0	0.0021118937632961313	0.0	0.07276647288868114	2.1118937632961312E-4
64-65	0.0	0.002370014112143436	0.0	0.07728357899350898	2.1118937632961312E-4
66-67	0.0	0.002393479598402282	0.0	0.08217613287847836	2.1118937632961312E-4
68-69	0.0	0.002393479598402282	0.0	0.08718601419474195	2.1118937632961312E-4
70-71	0.0	0.002405212341531705	0.0	0.09101088845493382	2.2292211945903605E-4
72-73	0.0	0.0024286778277905507	0.0	0.09434298750368994	2.8158583510615084E-4
74-75	0.0	0.0024521433140493967	0.0	0.09691245824903358	2.8158583510615084E-4
76-77	0.0	0.0024990742865670886	0.0	0.09957579093941259	2.8158583510615084E-4
78-79	0.0	0.0025108070296965116	0.0	0.1015234262988968	2.8158583510615084E-4
80-81	0.0	0.0025108070296965116	0.0	0.10198100328094428	2.933185782355738E-4
82-83	0.0	0.002534272515955357	0.0	0.10253244220802717	3.050513213649967E-4
84-85	0.0	0.002557738002214203	0.0	0.10276709707061563	3.050513213649967E-4
86-87	0.0	0.002557738002214203	0.0	0.10287269175878043	3.050513213649967E-4
88	0.0	0.002651599947249587	0.0	0.10287269175878043	3.050513213649967E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4900	0.0	50.67215	1
CTTATAC	5020	0.0	49.885784	1
TATACAC	7015	0.0	39.223736	3
GTATCAA	8995	0.0	38.27496	1
TTATACA	7335	0.0	35.304573	2
ATCAACG	10540	0.0	32.379215	3
TCAACGC	10565	0.0	32.343353	4
CAACGCA	10850	0.0	31.453726	5
TATCAAC	11125	0.0	30.870115	2
AACGCAG	11215	0.0	30.505402	6
ACATCTC	9145	0.0	28.594229	8
ACACATC	9255	0.0	27.88564	6
TACACAT	9855	0.0	27.184093	5
ACGCAGA	13040	0.0	26.203337	7
CGCAGAG	13075	0.0	26.198444	8
CACATCT	10145	0.0	25.35519	7
CATCTCC	10240	0.0	24.95333	9
ATACACA	12380	0.0	23.087461	4
TATGCCG	6610	0.0	22.47889	42-43
CGTATGC	6715	0.0	21.939646	40-41
>>END_MODULE