FastQCFastQC Report
Fri 27 May 2016
SRR2140063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4756066
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67750.050.739191
GTATCAA123000.037.2752571
CTTATAC41550.030.4754091
ATCAACG149400.030.1461723
TCAACGC149400.030.1177314
CAACGCA150050.030.0146355
TATCAAC155650.029.1013012
AACGCAG155800.028.9338746
ACGCAGA179150.025.1626997
CGCAGAG181400.024.803758
TATACAC62500.024.1337743
GTGGTAT28750.022.7607821
ACATCTC59600.021.3862028
GCAGAGT213200.021.1639129
TGGTATC30100.021.0341072
TTATACA68400.020.0655352
GTACATG162700.019.9269731
ACACATC63150.019.9148486
GAGTACT122900.019.86059412-13
TACATGG159700.019.7425542
Produced by FastQC (version 0.11.5)