##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140063_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4756066 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.376168034674034 31.0 18.0 33.0 18.0 33.0 2 30.331958597715 32.0 28.0 33.0 25.0 33.0 3 31.390085839851675 33.0 31.0 33.0 29.0 33.0 4 32.45598000532373 33.0 33.0 33.0 31.0 34.0 5 32.935265616583116 33.0 33.0 34.0 32.0 34.0 6 37.11107457297691 38.0 37.0 38.0 36.0 38.0 7 37.23292233539232 38.0 38.0 38.0 36.0 38.0 8 37.463950878730444 38.0 38.0 38.0 37.0 38.0 9 37.50994876858311 38.0 38.0 38.0 37.0 38.0 10-11 37.52552771555315 38.0 38.0 38.0 37.5 38.0 12-13 37.54607989039681 38.0 38.0 38.0 37.5 38.0 14-15 37.55186639546213 38.0 38.0 38.0 38.0 38.0 16-17 37.48980806826482 38.0 38.0 38.0 37.5 38.0 18-19 37.52199633058078 38.0 38.0 38.0 37.0 38.0 20-21 37.54731396288531 38.0 38.0 38.0 38.0 38.0 22-23 37.55249536653643 38.0 38.0 38.0 38.0 38.0 24-25 37.550075806845925 38.0 38.0 38.0 38.0 38.0 26-27 37.51967677002946 38.0 38.0 38.0 38.0 38.0 28-29 37.49275452739006 38.0 38.0 38.0 37.0 38.0 30-31 37.39826053489227 38.0 38.0 38.0 37.0 38.0 32-33 37.455544593185365 38.0 38.0 38.0 37.0 38.0 34-35 37.46508097894186 38.0 38.0 38.0 37.0 38.0 36-37 37.44626220535953 38.0 38.0 38.0 37.0 38.0 38-39 37.41535334697753 38.0 38.0 38.0 37.0 38.0 40-41 37.40974637891286 38.0 38.0 38.0 37.0 38.0 42-43 37.38321853361184 38.0 38.0 38.0 37.0 38.0 44-45 37.34347311835175 38.0 38.0 38.0 37.0 38.0 46-47 37.30873141428371 38.0 38.0 38.0 37.0 38.0 48-49 37.27531973357234 38.0 38.0 38.0 37.0 38.0 50-51 37.233038015275646 38.0 38.0 38.0 37.0 38.0 52-53 37.211857217842066 38.0 38.0 38.0 37.0 38.0 54-55 37.199914042176836 38.0 38.0 38.0 37.0 38.0 56-57 37.19292223286114 38.0 38.0 38.0 37.0 38.0 58-59 37.18485570525199 38.0 38.0 38.0 37.0 38.0 60-61 37.18770335756797 38.0 38.0 38.0 37.0 38.0 62-63 37.19674587644964 38.0 38.0 38.0 37.0 38.0 64-65 37.199644365148885 38.0 38.0 38.0 37.0 38.0 66-67 37.18293202704832 38.0 38.0 38.0 37.0 38.0 68-69 37.18694046362517 38.0 38.0 38.0 37.0 38.0 70-71 37.171363736070674 38.0 38.0 38.0 36.5 38.0 72-73 37.16278075726716 38.0 38.0 38.0 36.0 38.0 74-75 37.155520154128524 38.0 38.0 38.0 36.0 38.0 76-77 37.1615603141656 38.0 38.0 38.0 36.0 38.0 78-79 37.150539419381715 38.0 38.0 38.0 36.0 38.0 80-81 37.15158038725732 38.0 38.0 38.0 36.0 38.0 82-83 37.1052880647409 38.0 38.0 38.0 36.0 38.0 84-85 37.09972412258229 38.0 38.0 38.0 36.0 38.0 86-87 37.09470963360357 38.0 38.0 38.0 36.0 38.0 88-89 37.10471284592202 38.0 38.0 38.0 36.0 38.0 90-91 37.09667409152101 38.0 38.0 38.0 36.0 38.0 92-93 37.08256234013753 38.0 38.0 38.0 36.0 38.0 94-95 37.053953340243 38.0 38.0 38.0 36.0 38.0 96-97 37.025880540960145 38.0 38.0 38.0 36.0 38.0 98-99 37.030699276133475 38.0 38.0 38.0 36.0 38.0 100 35.49241007091868 38.0 36.0 38.0 29.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 9.0 19 19.0 20 37.0 21 107.0 22 239.0 23 734.0 24 1678.0 25 3329.0 26 5930.0 27 9571.0 28 15214.0 29 23208.0 30 31892.0 31 42163.0 32 54622.0 33 71274.0 34 105044.0 35 195133.0 36 658653.0 37 3537210.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.017190678178146 21.7927799992683 14.419501327357526 23.77052799519603 2 13.787322357720585 21.295709670195418 42.19536786947018 22.721600102613813 3 17.18756636261986 26.88936612738343 31.69030875517707 24.232758754819635 4 12.011200436679937 18.907812340329436 39.89689828561912 29.1840889373715 5 12.71067641645768 38.122777643325776 36.068710837057985 13.097835103158562 6 28.365733360302404 39.092750184711484 19.38597992542576 13.155536529560354 7 25.180916328747333 33.56275964210758 23.900215009631907 17.356109019513184 8 23.55734760619386 37.139160810636355 21.989602331002132 17.31388925216765 9 25.11599429190549 17.053531573344536 22.02120119351067 35.8092729412393 10-11 23.22261214700322 27.449243284152768 29.74707664171842 19.58106792712559 12-13 24.237153142954703 25.111846639638724 29.73441705813166 20.91658315927491 14-15 21.515176618659204 26.484083694381027 27.457629898323532 24.54310978863624 16-17 20.89310787529021 29.596572040842155 28.175040043599058 21.335280040268575 18-19 20.875624938762414 28.57688476148144 30.074151620267674 20.473338679488467 20-21 21.810096928928143 27.65020178151415 29.876542534967072 20.66315875459064 22-23 21.47602221652395 27.784311863997647 29.66724311710502 21.07242280237338 24-25 21.20932160299979 27.84362387063156 29.90610518187863 21.04094934449002 26-27 21.12347305857676 27.991675874336032 29.887232513174293 20.99761855391292 28-29 20.972055436386082 27.918255398180786 30.036818879137467 21.07287028629566 30-31 21.31380589235056 27.73986508543255 29.77296611972141 21.173362902495477 32-33 21.244456198241053 27.83860695841175 29.882075556321947 21.03486128702525 34-35 21.323645603778875 28.049154417558743 29.74583363454018 20.881366344122203 36-37 21.129816298052432 27.929192733556068 29.749013802093742 21.191977166297754 38-39 21.06792865703491 27.926616598989 29.894321888177483 21.11113285579861 40-41 21.278761697746745 27.783863258056236 29.87693720175421 21.06043784244281 42-43 21.32793981460664 27.82839829764808 29.692310909469345 21.151350978275932 44-45 21.256933142369988 28.062426840645138 29.48923494017615 21.19140507680872 46-47 21.435242712390014 27.890669482934094 29.19718302718329 21.47690477749261 48-49 21.399914567203705 27.963593306526136 29.21132139135127 21.425170734918893 50-51 21.263522525421163 28.104120712163816 29.197653305006536 21.43470345740848 52-53 21.39727359320287 28.076408475658894 29.246032764219493 21.28028516691875 54-55 21.40802850918697 28.07040792015039 29.05175573717649 21.469807833486147 56-57 21.557493388066995 28.231675282398587 28.842084478039737 21.36874685149468 58-59 21.3282995029365 28.36001975406216 29.120625358227905 21.191055384773442 60-61 21.51442778690257 28.555459648063042 28.79399752969646 21.136115035337927 62-63 21.351607695301226 28.68558545823216 28.767228678186946 21.19557816827967 64-65 21.31500111558217 28.644590283695997 28.790032672151472 21.250375928570357 66-67 21.381558179083072 28.634466234306146 28.625242425623572 21.35873316098721 68-69 21.423455140697047 28.53784967595975 28.73115539708455 21.30753978625865 70-71 21.429156255977798 28.294801695739558 28.77955336608039 21.49648868220226 72-73 21.524637954540676 28.135903404938826 28.819461855743732 21.519996784776765 74-75 21.486079508690022 28.217820289403488 28.74061612261642 21.555484079290075 76-77 21.526274353569004 28.180528056364583 28.683310188548056 21.609887401518357 78-79 21.538721700788628 28.169978348340436 28.672115308677178 21.619184642193762 80-81 21.51919695554205 28.201183523957486 28.622582321256658 21.65703719924381 82-83 21.595179422986778 28.1670782453393 28.68863964301333 21.549102688660593 84-85 21.702803877523674 28.142326141166553 28.576279567335007 21.57859041397477 86-87 21.56329547853061 28.154340763803752 28.635068286086014 21.64729547157962 88-89 21.66857216992224 28.119484514069644 28.606509523462016 21.6054337925461 90-91 21.66608258655821 28.152720455615288 28.59876266137149 21.58243429645501 92-93 21.673793666612337 28.11854804673476 28.61267565037762 21.594982636275276 94-95 21.590687224723823 28.261272722085256 28.52770286032683 21.620337192864092 96-97 21.68865661838072 28.344960359536103 28.590731662048686 21.375651360034496 98-99 22.737765593797217 30.285655633577218 28.78416311641511 18.192415656210457 100 21.569396039834515 31.15195640868411 22.835537737484444 24.443109813996934 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1124.0 1 1016.0 2 1315.5 3 1908.0 4 2371.0 5 3084.5 6 3799.0 7 4934.0 8 6434.5 9 7406.5 10 7638.5 11 7806.0 12 8652.5 13 10383.5 14 13431.5 15 16660.0 16 18826.5 17 19736.5 18 19496.5 19 18445.0 20 17326.0 21 17156.5 22 18816.5 23 22689.5 24 28984.0 25 37424.5 26 48129.0 27 60357.5 28 72703.0 29 85856.5 30 98440.0 31 110154.5 32 121834.0 33 132939.0 34 144015.0 35 154148.0 36 164382.0 37 173784.5 38 180523.0 39 186303.0 40 191560.0 41 195087.0 42 196939.0 43 199506.5 44 205721.0 45 212277.5 46 207840.5 47 200372.5 48 195058.0 49 186011.0 50 177826.5 51 169027.0 52 157926.0 53 145884.0 54 133853.0 55 122322.0 56 111393.0 57 99399.5 58 86197.0 59 73765.0 60 61692.0 61 49707.0 62 38673.5 63 29664.0 64 22219.0 65 16225.0 66 11761.5 67 8658.5 68 6448.5 69 4604.0 70 3284.5 71 2386.5 72 1673.0 73 1104.5 74 751.0 75 504.5 76 339.5 77 225.5 78 143.0 79 87.5 80 57.0 81 39.0 82 26.5 83 18.0 84 12.0 85 9.0 86 5.5 87 3.5 88 2.5 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0078005645842593434 3 0.0 4 0.0037846404991015684 5 0.0028384803743261764 6 0.0 7 0.0 8 0.0 9 2.733351471573355E-4 10-11 4.3102850128656745E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.67224646650233E-4 24-25 0.0016506811688294621 26-27 1.051503813436305E-5 28-29 0.002335180931291193 30-31 0.0034538284213856004 32-33 5.593106169502143E-4 34-35 1.0584134128921527E-5 36-37 0.0011373353720335608 38-39 0.002277793248235833 40-41 0.0017759217302859233 42-43 0.0 44-45 1.09213430674536E-5 46-47 0.0015209728936178655 48-49 5.115710134522047E-4 50-51 0.0017831029130742936 52-53 0.0027579119577298 54-55 0.0019483693515804008 56-57 0.001953859717699452 58-59 0.002179902987359925 60-61 8.773138568097452E-4 62-63 7.07986488785848E-4 64-65 0.0033454439993509124 66-67 0.0026696621398255987 68-69 9.725452896106146E-5 70-71 0.0033068876569127413 72-73 0.01062503444738188 74-75 0.012035042828672246 76-77 0.00527787437669382 78-79 0.004704988865073355 80-81 4.1463334362142317E-4 82-83 1.0474949942832961E-4 84-85 2.6456534757470963E-5 86-87 0.0017911835539407574 88-89 0.0045946194303753 90-91 4.783784555838589E-4 92-93 0.0010782659327269884 94-95 0.0018740666378936834 96-97 0.0024158732536256216 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 32.0 20-21 107.0 22-23 242.0 24-25 399.0 26-27 1243.0 28-29 3355.0 30-31 9837.0 32-33 12820.0 34-35 17816.0 36-37 26977.0 38-39 30437.0 40-41 30090.0 42-43 34593.0 44-45 41078.0 46-47 41315.0 48-49 38187.0 50-51 35418.0 52-53 34715.0 54-55 37440.0 56-57 38356.0 58-59 37833.0 60-61 37386.0 62-63 36880.0 64-65 40544.0 66-67 44252.0 68-69 45372.0 70-71 50264.0 72-73 39517.0 74-75 38568.0 76-77 40639.0 78-79 41254.0 80-81 40667.0 82-83 39099.0 84-85 38813.0 86-87 40233.0 88-89 41459.0 90-91 41619.0 92-93 41200.0 94-95 46846.0 96-97 140631.0 98-99 306541.0 100-101 3091992.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.25575040821326 #Duplication Level Percentage of deduplicated Percentage of total 1 91.88195829741717 82.00993136812129 2 6.1000878990746745 10.889358459759425 3 1.2811657911977148 3.4305424227205292 4 0.39692350850460983 1.417108224249591 5 0.1538174771380875 0.6864547173929094 6 0.06508692823757226 0.3485629572966023 7 0.03961321150644277 0.24749948433607727 8 0.023632478299235978 0.1687467667683298 9 0.01562647407231447 0.1255277402603025 >10 0.04082707920566239 0.5337253565517563 >50 8.159748038378009E-4 0.04962036693048032 >100 3.822345538962535E-4 0.06297957461708516 >500 6.264598875204699E-5 0.029942560995654666 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.102578055056427E-5 2 0.0 2.102578055056427E-5 0.0 0.0 2.102578055056427E-5 3 0.0 2.102578055056427E-5 0.0 0.0 2.102578055056427E-5 4 0.0 4.205156110112854E-5 0.0 0.0 2.102578055056427E-5 5 2.102578055056427E-5 4.205156110112854E-5 0.0 0.0 2.102578055056427E-5 6 4.205156110112854E-5 4.205156110112854E-5 0.0 0.0 6.307734165169281E-5 7 4.205156110112854E-5 4.205156110112854E-5 0.0 0.0 6.307734165169281E-5 8 4.205156110112854E-5 4.205156110112854E-5 0.0 0.0 6.307734165169281E-5 9 4.205156110112854E-5 1.2615468330338562E-4 0.0 0.0 6.307734165169281E-5 10-11 1.0512890275282135E-4 1.2615468330338562E-4 0.0 0.0 6.307734165169281E-5 12-13 1.2615468330338562E-4 1.471804638539499E-4 0.0 0.0 6.307734165169281E-5 14-15 1.2615468330338562E-4 2.2077069578092483E-4 0.0 0.0 6.307734165169281E-5 16-17 1.2615468330338562E-4 2.3128358605620697E-4 0.0 0.0 6.307734165169281E-5 18-19 1.2615468330338562E-4 2.417964763314891E-4 0.0 2.102578055056427E-5 6.307734165169281E-5 20-21 1.2615468330338562E-4 2.628222568820534E-4 0.0 2.102578055056427E-5 7.359023192697494E-5 22-23 1.2615468330338562E-4 3.469253790843105E-4 0.0 3.1538670825846405E-5 1.0512890275282135E-4 24-25 1.787191346797963E-4 3.88976940185439E-4 0.0 4.205156110112854E-5 1.1564179302810348E-4 26-27 1.8923202495507843E-4 3.9948983046072113E-4 0.0 8.410312220225708E-5 1.2615468330338562E-4 28-29 1.8923202495507843E-4 3.9948983046072113E-4 0.0 2.838480374326176E-4 1.2615468330338562E-4 30-31 1.8923202495507843E-4 4.205156110112854E-4 0.0 0.0011459050400057526 1.3666757357866775E-4 32-33 1.8923202495507843E-4 4.205156110112854E-4 0.0 0.002975147947904844 1.471804638539499E-4 34-35 1.9974491523036056E-4 4.7308006238769605E-4 0.0 0.005351061150118607 1.471804638539499E-4 36-37 2.102578055056427E-4 5.256445137641067E-4 0.0 0.008326209098023452 1.471804638539499E-4 38-39 2.102578055056427E-4 5.782089651405174E-4 0.0 0.012972906599698155 1.471804638539499E-4 40-41 2.102578055056427E-4 6.307734165169281E-4 0.0 0.01827140329844035 1.471804638539499E-4 42-43 2.3128358605620697E-4 6.517991970674923E-4 0.0 0.022939126580665617 1.471804638539499E-4 44-45 2.3128358605620697E-4 7.359023192697494E-4 0.0 0.028826345134823614 1.5769335412923202E-4 46-47 2.3128358605620697E-4 7.989796609214423E-4 0.0 0.035459978898526645 2.102578055056427E-4 48-49 2.3128358605620697E-4 8.094925511967243E-4 0.0 0.04174668728314536 2.102578055056427E-4 50-51 2.3128358605620697E-4 8.830827831236992E-4 0.0 0.04746569959289884 2.102578055056427E-4 52-53 2.733351471573355E-4 9.041085636742636E-4 0.0 0.05277470918191632 2.3128358605620697E-4 54-55 2.733351471573355E-4 9.041085636742636E-4 0.0 0.05876705663882713 2.628222568820534E-4 56-57 2.733351471573355E-4 9.041085636742636E-4 0.0 0.06455965918050759 2.838480374326176E-4 58-59 2.733351471573355E-4 9.041085636742636E-4 0.0 0.07066764843044651 3.1538670825846405E-4 60-61 2.733351471573355E-4 9.041085636742636E-4 0.0 0.07647076386240224 3.1538670825846405E-4 62-63 2.733351471573355E-4 9.251343442248279E-4 0.0 0.08253670155124004 3.574382693595926E-4 64-65 2.733351471573355E-4 0.0010512890275282134 0.0 0.08782468535970694 3.574382693595926E-4 66-67 2.733351471573355E-4 0.0010512890275282134 0.0 0.09345959454725816 3.574382693595926E-4 68-69 2.733351471573355E-4 0.0010723148080787777 0.0 0.09932578732086561 3.574382693595926E-4 70-71 2.733351471573355E-4 0.001093340588629342 0.0 0.10381479146841108 3.574382693595926E-4 72-73 2.733351471573355E-4 0.001093340588629342 0.0 0.10770456087026548 3.7846404991015686E-4 74-75 2.733351471573355E-4 0.0011143663691799061 0.0 0.11093201818477708 3.7846404991015686E-4 76-77 2.733351471573355E-4 0.0011564179302810348 0.0 0.11387562746185609 3.7846404991015686E-4 78-79 2.733351471573355E-4 0.0011774437108315991 0.0 0.11578897349195744 3.7846404991015686E-4 80-81 2.733351471573355E-4 0.0011774437108315991 0.0 0.11636718245709796 3.7846404991015686E-4 82-83 2.733351471573355E-4 0.0011774437108315991 0.0 0.11693487853196319 3.9948983046072113E-4 84-85 2.733351471573355E-4 0.0011984694913821632 0.0 0.11702949454444073 3.9948983046072113E-4 86-87 2.733351471573355E-4 0.0012405210524832919 0.0 0.1171556492277441 3.9948983046072113E-4 88 2.733351471573355E-4 0.0013456499552361133 0.0 0.11717667500829466 4.205156110112854E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6775 0.0 50.73919 1 GTATCAA 12300 0.0 37.275257 1 CTTATAC 4155 0.0 30.475409 1 ATCAACG 14940 0.0 30.146172 3 TCAACGC 14940 0.0 30.117731 4 CAACGCA 15005 0.0 30.014635 5 TATCAAC 15565 0.0 29.101301 2 AACGCAG 15580 0.0 28.933874 6 ACGCAGA 17915 0.0 25.162699 7 CGCAGAG 18140 0.0 24.80375 8 TATACAC 6250 0.0 24.133774 3 GTGGTAT 2875 0.0 22.760782 1 ACATCTC 5960 0.0 21.386202 8 GCAGAGT 21320 0.0 21.163912 9 TGGTATC 3010 0.0 21.034107 2 TTATACA 6840 0.0 20.065535 2 GTACATG 16270 0.0 19.926973 1 ACACATC 6315 0.0 19.914848 6 GAGTACT 12290 0.0 19.860594 12-13 TACATGG 15970 0.0 19.742554 2 >>END_MODULE