FastQCFastQC Report
Fri 27 May 2016
SRR2140062_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140062_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4091184
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58200.053.933841
GTATCAA108100.037.1143721
ATCAACG127900.030.966883
TCAACGC129300.030.6648964
CAACGCA130900.030.3547745
TATCAAC131950.030.0508772
AACGCAG135250.029.3151516
ACGCAGA157600.025.1578317
CGCAGAG158500.025.0149788
GTGGTAT22650.022.1886391
GCAGAGT185700.021.37399
TGGTATC22000.021.2954522
GAGTACT101600.020.72750512-13
TACATGG145000.019.6799352
CAGAGTA181450.019.43347410-11
GTACATG149600.019.2171541
ACATGGG148900.018.762653
GTACTTT113700.018.40953314-15
AGAGTAC169600.018.2551610-11
CATGGGG100750.016.5703374
Produced by FastQC (version 0.11.5)