##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140062_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4091184
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.304187980789912	31.0	18.0	33.0	18.0	33.0
2	30.23414493212723	32.0	27.0	33.0	25.0	33.0
3	31.324142839823384	33.0	31.0	33.0	28.0	33.0
4	32.43056753252848	33.0	33.0	33.0	31.0	34.0
5	32.91886676326462	33.0	33.0	34.0	32.0	34.0
6	37.092293820077515	38.0	37.0	38.0	36.0	38.0
7	37.20754089769612	38.0	38.0	38.0	36.0	38.0
8	37.44134020860465	38.0	38.0	38.0	37.0	38.0
9	37.48875117814305	38.0	38.0	38.0	37.0	38.0
10-11	37.51018054920043	38.0	38.0	38.0	37.0	38.0
12-13	37.52911418797101	38.0	38.0	38.0	37.5	38.0
14-15	37.53821314318789	38.0	38.0	38.0	38.0	38.0
16-17	37.47701276696428	38.0	38.0	38.0	37.5	38.0
18-19	37.508284398843955	38.0	38.0	38.0	37.0	38.0
20-21	37.53201413292039	38.0	38.0	38.0	37.0	38.0
22-23	37.54119744357466	38.0	38.0	38.0	38.0	38.0
24-25	37.54097006058481	38.0	38.0	38.0	38.0	38.0
26-27	37.51064928664846	38.0	38.0	38.0	37.5	38.0
28-29	37.4845108143748	38.0	38.0	38.0	37.0	38.0
30-31	37.38862982370918	38.0	38.0	38.0	37.0	38.0
32-33	37.45235942637507	38.0	38.0	38.0	37.0	38.0
34-35	37.462011729365315	38.0	38.0	38.0	37.0	38.0
36-37	37.441960249563984	38.0	38.0	38.0	37.0	38.0
38-39	37.4206457972394	38.0	38.0	38.0	37.0	38.0
40-41	37.405944889934005	38.0	38.0	38.0	37.0	38.0
42-43	37.378604325005696	38.0	38.0	38.0	37.0	38.0
44-45	37.340481021840674	38.0	38.0	38.0	37.0	38.0
46-47	37.306128473749794	38.0	38.0	38.0	37.0	38.0
48-49	37.27278139995634	38.0	38.0	38.0	37.0	38.0
50-51	37.22650754168116	38.0	38.0	38.0	37.0	38.0
52-53	37.20570194220238	38.0	38.0	38.0	37.0	38.0
54-55	37.19521135115471	38.0	38.0	38.0	37.0	38.0
56-57	37.18581190338359	38.0	38.0	38.0	37.0	38.0
58-59	37.17888876340005	38.0	38.0	38.0	37.0	38.0
60-61	37.18088309213998	38.0	38.0	38.0	37.0	38.0
62-63	37.189858282007975	38.0	38.0	38.0	37.0	38.0
64-65	37.19537806354158	38.0	38.0	38.0	37.0	38.0
66-67	37.178452841211325	38.0	38.0	38.0	37.0	38.0
68-69	37.18199014468438	38.0	38.0	38.0	36.5	38.0
70-71	37.167261178390845	38.0	38.0	38.0	36.0	38.0
72-73	37.16118711125437	38.0	38.0	38.0	36.0	38.0
74-75	37.15531658709325	38.0	38.0	38.0	36.0	38.0
76-77	37.15981327096998	38.0	38.0	38.0	36.0	38.0
78-79	37.1468177527571	38.0	38.0	38.0	36.0	38.0
80-81	37.144067969213076	38.0	38.0	38.0	36.0	38.0
82-83	37.09609430914958	38.0	38.0	38.0	36.0	38.0
84-85	37.08792196128145	38.0	38.0	38.0	36.0	38.0
86-87	37.08248431197913	38.0	38.0	38.0	36.0	38.0
88-89	37.08835832871364	38.0	38.0	38.0	36.0	38.0
90-91	37.08035664634908	38.0	38.0	38.0	36.0	38.0
92-93	37.06777055709381	38.0	38.0	38.0	36.0	38.0
94-95	37.03869032734296	38.0	38.0	38.0	36.0	38.0
96-97	37.00737939193692	38.0	38.0	38.0	36.0	38.0
98-99	37.01351915192425	38.0	38.0	38.0	36.0	38.0
100	35.48060372990834	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	4.0
18	5.0
19	11.0
20	38.0
21	87.0
22	209.0
23	603.0
24	1394.0
25	2687.0
26	4949.0
27	8048.0
28	13027.0
29	19541.0
30	27422.0
31	36774.0
32	47387.0
33	62720.0
34	92305.0
35	171916.0
36	574814.0
37	3027242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.32514792783703	21.47960101525622	14.433034544523052	23.762216512383702
2	13.921669616708096	21.106073646820764	42.02541590450812	22.946840831963016
3	17.477018877664754	26.574825282852103	31.313917926937535	24.634237912545608
4	12.136138408751085	18.555526467405507	39.812242102383934	29.49609302145948
5	12.893118810598509	37.96897923426315	35.77248570421499	13.365416250923353
6	28.764289261983816	38.75921982487222	19.319786154814842	13.15670475832913
7	25.641916863186793	33.37332175722236	23.72643225041944	17.25832912917141
8	23.75058173868494	37.12546294666777	21.71982487221303	17.40413044243427
9	25.382928636254643	17.05061172032332	21.92525995638897	35.641199687033065
10-11	23.467294264419916	27.345135133582676	29.452339320761578	19.735231281235826
12-13	24.463104624859774	25.015133151568484	29.56494129388232	20.956820929689414
14-15	21.704878098858423	26.278211881939313	27.408117063422228	24.608792955780036
16-17	21.05391739897301	29.4072571656518	28.07428118608207	21.464544249293112
18-19	21.037724042722104	28.465549337306754	29.999934004434902	20.496792615536236
20-21	22.07430927038763	27.529481355464057	29.77935272399292	20.616856650155395
22-23	21.69948183742142	27.496740400988124	29.61133651137107	21.192441250219385
24-25	21.376839095148014	27.689247142682362	29.749153490352732	21.184760271816895
26-27	21.29982198891058	27.827119365462522	29.68777101830826	21.185287627318633
28-29	21.221723684155826	27.908383501664314	29.705492719977443	21.164400094202417
30-31	21.60583419848681	27.650717243037175	29.62316925486479	21.120279303611223
32-33	21.251287495587746	27.831686949755845	29.620971601385726	21.296053953270686
34-35	21.39211231056355	27.82378605403378	29.647848825947527	21.13625280945514
36-37	21.33601041691749	27.800438730533877	29.658542187219272	21.20500866532936
38-39	21.210926608005735	27.843151866983877	29.725754236398714	21.22016728861167
40-41	21.42558256727545	27.74899714240578	29.620531384543398	21.20488890577538
42-43	21.41256012907366	27.756648301065546	29.484141823546583	21.346649746314206
44-45	21.45959016087984	27.86684703042447	29.33798963195704	21.335573176738652
46-47	21.494435815405787	27.860530578236535	29.122155001691347	21.52287860466633
48-49	21.49498936304859	27.911814647525272	29.124797392169672	21.46839859725647
50-51	21.445778524050514	28.040287278573878	29.009778306517735	21.504155890857874
52-53	21.577780851533866	28.03330669654183	28.926441156399484	21.462471295524825
54-55	21.58627799311579	27.998461248342014	28.847642309750288	21.567618448791904
56-57	21.599481230597796	28.05850684517026	28.81778691266893	21.524225011563015
58-59	21.51492115932152	28.13396386691343	28.931844254243256	21.419270719521794
60-61	21.641499172403634	28.09185349706099	28.866949283489163	21.39969804704622
62-63	21.53630264842824	28.226613609530364	28.817821308844554	21.41926243319684
64-65	21.52864481855054	28.199430489660454	28.7608795068348	21.511045184954206
66-67	21.610224228069416	28.153053504736747	28.649922918073823	21.58679934912002
68-69	21.66423624795901	28.12502111367603	28.684533528517537	21.526209109847418
70-71	21.60249775071894	27.962882987471854	28.76980856901073	21.664810692798476
72-73	21.651567988311147	27.92162443807453	28.7580822139849	21.668725359629423
74-75	21.6185944913271	28.00063604242333	28.671193632843934	21.709575833405633
76-77	21.637100577248162	28.02357219967691	28.62231169701408	21.71701552606084
78-79	21.674943205040204	28.011879609340085	28.573737821113383	21.73943936450633
80-81	21.69652265857954	28.032284085108728	28.519827566088395	21.75136569022334
82-83	21.691107665059505	28.01901986673274	28.57581688102364	21.714055587184113
84-85	21.821042723918808	27.96978113821203	28.5015144709752	21.70766166689397
86-87	21.69047884679393	28.002284389168935	28.53670245244701	21.77053431159013
88-89	21.750482896728343	28.00916454771338	28.441482494125918	21.798870061432357
90-91	21.77236458185785	28.034756663292182	28.496006299317923	21.696872455532045
92-93	21.777372145766577	28.049639660432412	28.457949537626824	21.715038656174183
94-95	21.700436843572824	28.18530158213795	28.4069183680846	21.707343206204623
96-97	21.84454175851347	28.174445197218397	28.48853338720076	21.492479657067378
98-99	22.86281756963033	30.122869971674	28.74797152808128	18.266340930614398
100	21.698097709410643	31.037467097280043	22.75546511844979	24.50897007485952
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	858.0
1	817.0
2	1118.0
3	1614.5
4	1971.0
5	2478.5
6	3108.5
7	4126.5
8	5512.0
9	6521.5
10	6695.0
11	6692.5
12	7381.0
13	8784.5
14	11143.0
15	13750.5
16	15399.0
17	16050.0
18	15790.0
19	14752.5
20	13652.5
21	13533.5
22	15026.5
23	18210.0
24	23586.0
25	30896.5
26	39787.0
27	49968.0
28	60274.5
29	70856.5
30	80348.5
31	89731.5
32	100093.5
33	109771.0
34	119310.0
35	128264.5
36	137437.0
37	145360.0
38	151335.5
39	156174.0
40	161350.5
41	165970.5
42	168977.5
43	173227.0
44	174586.5
45	174097.5
46	173090.5
47	170527.0
48	167465.0
49	162616.5
50	157406.0
51	150678.5
52	141831.0
53	132124.5
54	121831.0
55	110911.0
56	100078.0
57	89164.0
58	78145.0
59	67272.5
60	56256.0
61	45441.5
62	35552.0
63	27370.0
64	20484.5
65	15014.0
66	10881.5
67	7997.0
68	5873.0
69	4150.5
70	2943.5
71	2079.0
72	1419.0
73	971.5
74	658.0
75	442.5
76	290.0
77	176.0
78	113.5
79	79.5
80	53.0
81	30.5
82	21.0
83	13.0
84	7.5
85	6.5
86	4.5
87	2.5
88	1.5
89	1.0
90	1.0
91	1.5
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006868427330572275
3	0.0
4	0.004179719122874943
5	0.002884250622802592
6	0.0
7	0.0
8	0.0
9	4.1552763209867853E-4
10-11	6.966198538124905E-4
12-13	1.222140094407878E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.777479238745022E-4
24-25	0.001478917842813767
26-27	0.0
28-29	0.002103125023690725
30-31	0.00299867360710693
32-33	5.151182294454335E-4
34-35	1.2298570930655E-5
36-37	0.0012594976308479124
38-39	0.002272857682594307
40-41	0.0016872319410253696
42-43	0.0
44-45	0.0
46-47	0.0014962934126513782
48-49	6.063844802730227E-4
50-51	0.0018474332387065887
52-53	0.0026220928365079227
54-55	0.002061493560013051
56-57	0.002159271030100238
58-59	0.0020977302961605214
60-61	9.089165253776278E-4
62-63	8.893554280235074E-4
64-65	0.003602618731132837
66-67	0.002591858748714696
68-69	8.445463280110962E-5
70-71	0.0032421018204828317
72-73	0.010143949249218442
74-75	0.012432039032830883
76-77	0.005053943892726305
78-79	0.004499873811104475
80-81	3.2911694632132524E-4
82-83	1.0582034577553842E-4
84-85	4.581667769830451E-5
86-87	0.0020214059171334677
88-89	0.0046949879989243645
90-91	7.728971165101392E-4
92-93	9.410545744662865E-4
94-95	0.0022751751763865863
96-97	0.0024429942058407893
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	24.0
20-21	87.0
22-23	195.0
24-25	271.0
26-27	945.0
28-29	2638.0
30-31	7993.0
32-33	10347.0
34-35	14324.0
36-37	22180.0
38-39	25359.0
40-41	25065.0
42-43	28824.0
44-45	34336.0
46-47	34940.0
48-49	32766.0
50-51	30061.0
52-53	29406.0
54-55	31908.0
56-57	33023.0
58-59	32664.0
60-61	31782.0
62-63	31554.0
64-65	33429.0
66-67	35745.0
68-69	35945.0
70-71	37065.0
72-73	33315.0
74-75	33358.0
76-77	34795.0
78-79	35568.0
80-81	35293.0
82-83	33737.0
84-85	33419.0
86-87	34293.0
88-89	35781.0
90-91	35180.0
92-93	35522.0
94-95	40354.0
96-97	123042.0
98-99	266301.0
100-101	2678350.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.45656694349435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.596840053718	83.75992261075183
2	5.739004347727499	10.382612619384354
3	1.0794678385732623	2.929348644097544
4	0.32377670143819137	1.1715091547355017
5	0.11539676571495219	0.5219197631478655
6	0.054282085108599484	0.2946102639275092
7	0.03207566442995038	0.20310181407354072
8	0.018080873611722496	0.13084270034045095
9	0.010234022009997276	0.0833161047343662
>10	0.02964051036323311	0.3839684941752748
>50	7.115185589783775E-4	0.043934149585475805
>100	4.896187455744097E-4	0.09491368104623879
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.444280188815756E-5	0.0	2.444280188815756E-5	0.0
3	0.0	2.444280188815756E-5	0.0	2.444280188815756E-5	0.0
4	0.0	7.332840566447268E-5	0.0	4.888560377631512E-5	0.0
5	0.0	7.332840566447268E-5	0.0	1.222140094407878E-4	0.0
6	0.0	7.332840566447268E-5	0.0	1.222140094407878E-4	4.888560377631512E-5
7	0.0	7.332840566447268E-5	0.0	1.7109961321710293E-4	7.332840566447268E-5
8	0.0	7.332840566447268E-5	0.0	1.7109961321710293E-4	7.332840566447268E-5
9	0.0	1.222140094407878E-4	0.0	3.1775642454604826E-4	7.332840566447268E-5
10-11	0.0	1.222140094407878E-4	0.0	3.7886342926644217E-4	9.777120755263024E-5
12-13	0.0	1.222140094407878E-4	0.0	4.1552763209867853E-4	1.222140094407878E-4
14-15	0.0	1.222140094407878E-4	0.0	4.6441323587499364E-4	1.955424151052605E-4
16-17	0.0	1.222140094407878E-4	0.0	4.888560377631512E-4	1.955424151052605E-4
18-19	0.0	1.222140094407878E-4	0.0	5.744058443717026E-4	2.1998521699341804E-4
20-21	0.0	1.222140094407878E-4	0.0	7.577268585328843E-4	2.1998521699341804E-4
22-23	0.0	1.4665681132894535E-4	0.0	8.066124623091994E-4	2.1998521699341804E-4
24-25	0.0	1.7109961321710293E-4	0.0	8.432766651414359E-4	2.1998521699341804E-4
26-27	0.0	1.7109961321710293E-4	0.0	9.532692736381449E-4	2.1998521699341804E-4
28-29	0.0	1.833210141611817E-4	0.0	0.0011732544906315628	2.1998521699341804E-4
30-31	0.0	1.955424151052605E-4	0.0	0.002016531155772999	2.1998521699341804E-4
32-33	0.0	1.955424151052605E-4	0.0	0.004558582552141385	2.1998521699341804E-4
34-35	0.0	2.1998521699341804E-4	0.0	0.007760589599490025	2.5664941982565435E-4
36-37	0.0	2.444280188815756E-4	0.0	0.012575821571457065	2.6887082076973315E-4
38-39	0.0	2.444280188815756E-4	0.0	0.019578684312414205	2.6887082076973315E-4
40-41	0.0	3.91084830210521E-4	0.0	0.02644711164298648	2.933136226578907E-4
42-43	0.0	4.1552763209867853E-4	0.0	0.033596631195272564	2.933136226578907E-4
44-45	0.0	4.7663463681907244E-4	0.0	0.0416383130164764	2.933136226578907E-4
46-47	0.0	5.132988396513088E-4	0.0	0.05047438589904536	2.933136226578907E-4
48-49	0.0	5.132988396513088E-4	0.0	0.05907825216367682	2.933136226578907E-4
50-51	0.0	5.377416415394663E-4	0.0	0.06793876784813394	2.933136226578907E-4
52-53	0.0	5.621844434276239E-4	0.0	0.07618821348538712	3.1775642454604826E-4
54-55	0.0	5.621844434276239E-4	0.0	0.0836310466603311	3.4219922643420587E-4
56-57	0.0	5.621844434276239E-4	0.0	0.08893513467006128	3.4219922643420587E-4
58-59	0.0	6.110700472039389E-4	0.0	0.09431255108545594	3.4219922643420587E-4
60-61	0.0	6.355128490920965E-4	0.0	0.09924999706686377	3.4219922643420587E-4
62-63	0.0	6.355128490920965E-4	0.0	0.103784136817117	3.4219922643420587E-4
64-65	0.0	6.843984528684117E-4	0.0	0.10835494077020247	3.4219922643420587E-4
66-67	0.0	7.088412547565692E-4	0.0	0.11362236457710043	3.4219922643420587E-4
68-69	0.0	7.088412547565692E-4	0.0	0.11847426075189971	3.4219922643420587E-4
70-71	2.444280188815756E-5	7.088412547565692E-4	0.0	0.12328949272386674	3.4219922643420587E-4
72-73	2.444280188815756E-5	7.088412547565692E-4	0.0	0.12788473947884035	3.4219922643420587E-4
74-75	2.444280188815756E-5	7.088412547565692E-4	0.0	0.13031679826671203	3.4219922643420587E-4
76-77	2.444280188815756E-5	7.088412547565692E-4	0.0	0.13317660608762646	3.4219922643420587E-4
78-79	2.444280188815756E-5	7.21062655700648E-4	0.0	0.13525424424811988	3.4219922643420587E-4
80-81	2.444280188815756E-5	7.332840566447268E-4	0.0	0.13604863530948497	3.4219922643420587E-4
82-83	2.444280188815756E-5	7.332840566447268E-4	0.0	0.13652526994630404	3.4219922643420587E-4
84-85	2.444280188815756E-5	7.332840566447268E-4	0.0	0.1367330337623534	3.4219922643420587E-4
86-87	2.444280188815756E-5	7.332840566447268E-4	0.0	0.13687969057368235	3.4219922643420587E-4
88	2.444280188815756E-5	8.310552641973571E-4	0.0	0.13687969057368235	3.4219922643420587E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5820	0.0	53.93384	1
GTATCAA	10810	0.0	37.114372	1
ATCAACG	12790	0.0	30.96688	3
TCAACGC	12930	0.0	30.664896	4
CAACGCA	13090	0.0	30.354774	5
TATCAAC	13195	0.0	30.050877	2
AACGCAG	13525	0.0	29.315151	6
ACGCAGA	15760	0.0	25.157831	7
CGCAGAG	15850	0.0	25.014978	8
GTGGTAT	2265	0.0	22.188639	1
GCAGAGT	18570	0.0	21.3739	9
TGGTATC	2200	0.0	21.295452	2
GAGTACT	10160	0.0	20.727505	12-13
TACATGG	14500	0.0	19.679935	2
CAGAGTA	18145	0.0	19.433474	10-11
GTACATG	14960	0.0	19.217154	1
ACATGGG	14890	0.0	18.76265	3
GTACTTT	11370	0.0	18.409533	14-15
AGAGTAC	16960	0.0	18.25516	10-11
CATGGGG	10075	0.0	16.570337	4
>>END_MODULE