FastQCFastQC Report
Fri 27 May 2016
SRR2140061_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140061_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3974812
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46850.048.2521131
GTATCAA82700.037.9197241
CTTATAC37450.032.7914281
TCAACGC98750.031.1944244
ATCAACG98800.031.0926273
CAACGCA101650.030.218975
TATCAAC104900.029.813752
AACGCAG106050.029.0438526
TATACAC51600.027.6672253
ACGCAGA123050.024.9967677
CGCAGAG125250.024.5577018
TTATACA55600.023.9212472
ACACATC58400.022.3332756
ACATCTC59300.021.6361068
TACACAT64900.021.6031955
GTGGTAT21650.020.6084671
GCAGAGT148750.020.5637559
TATGCCG35800.019.74120942-43
CACATCT65200.019.222127
GAGTACT85450.019.11662312-13
Produced by FastQC (version 0.11.5)