##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140061_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3974812
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.6327584801495	31.0	18.0	33.0	18.0	33.0
2	30.424304344457045	32.0	29.0	33.0	25.0	33.0
3	31.426697916781976	33.0	31.0	33.0	29.0	33.0
4	32.44531162731722	33.0	33.0	33.0	31.0	34.0
5	32.92746222966017	33.0	33.0	34.0	32.0	34.0
6	37.094980592792815	38.0	37.0	38.0	36.0	38.0
7	37.20913341310231	38.0	38.0	38.0	36.0	38.0
8	37.44049026721264	38.0	38.0	38.0	37.0	38.0
9	37.49224793524826	38.0	38.0	38.0	37.0	38.0
10-11	37.50925842530414	38.0	38.0	38.0	37.0	38.0
12-13	37.53080233731809	38.0	38.0	38.0	37.5	38.0
14-15	37.53569439259014	38.0	38.0	38.0	38.0	38.0
16-17	37.47226988345612	38.0	38.0	38.0	37.5	38.0
18-19	37.506055254940364	38.0	38.0	38.0	37.0	38.0
20-21	37.52960618337664	38.0	38.0	38.0	37.0	38.0
22-23	37.538070803282935	38.0	38.0	38.0	38.0	38.0
24-25	37.535574742736586	38.0	38.0	38.0	38.0	38.0
26-27	37.50523668990471	38.0	38.0	38.0	37.5	38.0
28-29	37.4777011167974	38.0	38.0	38.0	37.0	38.0
30-31	37.38375736002007	38.0	38.0	38.0	37.0	38.0
32-33	37.444854773766664	38.0	38.0	38.0	37.0	38.0
34-35	37.45190197454969	38.0	38.0	38.0	37.0	38.0
36-37	37.43429059692552	38.0	38.0	38.0	37.0	38.0
38-39	37.40417154411992	38.0	38.0	38.0	37.0	38.0
40-41	37.406503051798715	38.0	38.0	38.0	37.0	38.0
42-43	37.385080483426094	38.0	38.0	38.0	37.0	38.0
44-45	37.353289339397406	38.0	38.0	38.0	37.0	38.0
46-47	37.32890454219752	38.0	38.0	38.0	37.0	38.0
48-49	37.30133209221479	38.0	38.0	38.0	37.0	38.0
50-51	37.26239565229973	38.0	38.0	38.0	37.0	38.0
52-53	37.24334296493083	38.0	38.0	38.0	37.0	38.0
54-55	37.23032230171128	38.0	38.0	38.0	37.0	38.0
56-57	37.2234630558203	38.0	38.0	38.0	37.0	38.0
58-59	37.213756747366276	38.0	38.0	38.0	37.0	38.0
60-61	37.213267703365005	38.0	38.0	38.0	37.0	38.0
62-63	37.221255079470794	38.0	38.0	38.0	37.0	38.0
64-65	37.2220632509301	38.0	38.0	38.0	37.0	38.0
66-67	37.20139815567076	38.0	38.0	38.0	37.0	38.0
68-69	37.20424894781165	38.0	38.0	38.0	37.0	38.0
70-71	37.18577558540244	38.0	38.0	38.0	36.5	38.0
72-73	37.17874755161246	38.0	38.0	38.0	36.0	38.0
74-75	37.169443651750356	38.0	38.0	38.0	36.0	38.0
76-77	37.17365457398094	38.0	38.0	38.0	36.0	38.0
78-79	37.160527134785205	38.0	38.0	38.0	36.0	38.0
80-81	37.16111348995113	38.0	38.0	38.0	36.0	38.0
82-83	37.11451670621733	38.0	38.0	38.0	36.0	38.0
84-85	37.10585249372983	38.0	38.0	38.0	36.0	38.0
86-87	37.09922386963391	38.0	38.0	38.0	36.0	38.0
88-89	37.10511365323521	38.0	38.0	38.0	36.0	38.0
90-91	37.09424552874251	38.0	38.0	38.0	36.0	38.0
92-93	37.07929420555529	38.0	38.0	38.0	36.0	38.0
94-95	37.052062027024505	38.0	38.0	38.0	36.0	38.0
96-97	37.02291189502296	38.0	38.0	38.0	36.0	38.0
98-99	37.024113780998874	38.0	38.0	38.0	36.0	38.0
100	35.51303051939103	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	5.0
19	6.0
20	39.0
21	78.0
22	218.0
23	612.0
24	1323.0
25	2644.0
26	4842.0
27	7915.0
28	12526.0
29	18402.0
30	25420.0
31	34037.0
32	44195.0
33	59443.0
34	88723.0
35	164940.0
36	550251.0
37	2959190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.03658537812606	21.30020237435129	14.30990950012227	24.35330274740038
2	14.000846390662128	20.864511071011272	41.75025738931254	23.38438514901407
3	17.367462913969263	26.130242134722348	31.427272535153865	25.075022416154525
4	12.425125595774828	18.497651117480356	39.05814742206621	30.0190758646786
5	13.246049311555852	37.453137819405654	35.79997619938375	13.500836669654742
6	29.35074665166554	38.41115001162319	18.918026814853132	13.320076521858141
7	25.801522185200206	32.895115542571574	23.476556878664955	17.82680539356327
8	23.881003680174057	37.03636800935491	21.58021561774494	17.502412692726097
9	25.124610043272614	17.05393982087149	21.56015397001107	36.26129616584482
10-11	23.728116795960545	26.990008289735695	29.334821041085135	19.947053873218618
12-13	24.623409767156225	24.45672259636999	29.279203464013758	21.640664172460024
14-15	21.854819800282378	25.90652086186718	26.81918037884559	25.419478959004856
16-17	21.58754929792906	29.094558434461803	27.46622984936143	21.851662418247706
18-19	21.598681899923818	28.116965028786268	29.16700211230116	21.11735095898875
20-21	22.3184722061137	27.301280046654192	28.9930730705144	21.387174676717713
22-23	22.02635258195776	27.4216224416859	28.83019061605343	21.72183436030291
24-25	21.86038588895751	27.501894295124075	28.955510263286577	21.68220955263184
26-27	21.88430631725554	27.63094424796464	28.904926002392983	21.57982343238683
28-29	21.57497312632158	27.532793061537795	29.015492655157182	21.876741156983435
30-31	22.03952676923922	27.303866872952298	29.07283208914845	21.583774268660033
32-33	21.7505523607156	27.66007351680638	28.835842510730984	21.753531611747036
34-35	21.77367717061144	27.67492079695994	29.060015054464195	21.491386977964428
36-37	21.967838623880258	27.50681008577867	28.86699672706559	21.65835456327548
38-39	21.69278601588978	27.48391289702387	29.0667907306981	21.756510356388254
40-41	21.934765452687454	27.361842968188764	28.99468248812342	21.708709091000365
42-43	21.96530428781742	27.378945559870875	28.868309136655018	21.787441015656682
44-45	21.90582437590749	27.60636574556214	28.722299971571463	21.765509906958904
46-47	22.119570108513827	27.398433900092645	28.39764712114143	22.0843488702521
48-49	21.96248376560795	27.454035235111217	28.487599383724532	22.095881615556305
50-51	21.847309865179266	27.600189320850987	28.539903819538786	22.012596994430957
52-53	22.014899512792987	27.583877140016593	28.560398714561373	21.84082463262905
54-55	21.966752966588174	27.574976745326254	28.3713965470044	22.08687374108117
56-57	22.138587302614468	27.73206526271564	28.156770547172144	21.972576887497752
58-59	21.87781942274858	27.94057301483526	28.441948518830902	21.73965904358526
60-61	22.08000825839719	28.12143704503806	28.074689817035374	21.723864879529373
62-63	21.903585140071467	28.340493374426927	27.994459620290314	21.76146186521129
64-65	21.896781716829953	28.28233792813955	27.990639783853844	21.830240571176656
66-67	21.949516213481353	28.24614772450967	27.865559303167753	21.93877675884123
68-69	21.9577914755687	28.10078820130909	28.027117724112216	21.91430259900999
70-71	22.012811527133678	27.80043349310179	28.056245685765525	22.130509293999005
72-73	22.111803058822055	27.657772086211903	28.102327019455547	22.128097835510495
74-75	22.001815277571374	27.7341374974792	28.06686804933624	22.197179175613183
76-77	22.148913978921875	27.680646638992627	27.990940550445142	22.179498831640355
78-79	22.12346317189475	27.755790581377372	27.930683023602327	22.190063223125556
80-81	22.139238224943476	27.74264849609474	27.9228779613264	22.195235317635383
82-83	22.183921631248925	27.667426950511558	27.972949779773348	22.175701638466172
84-85	22.275838783132766	27.651947205474986	27.971677242279195	22.10053676911305
86-87	22.117579533062763	27.65357120730263	27.997390816689144	22.231458442945463
88-89	22.21865048295558	27.64324977789317	27.924771706632374	22.213328032518874
90-91	22.24121824598627	27.68276118775898	27.957137661560118	22.11888290469463
92-93	22.299740757051826	27.645404384637057	27.908880345028674	22.145974513282447
94-95	22.19039474506258	27.725464721100163	27.891780830200037	22.192359703637223
96-97	22.313279883180407	27.82497063211232	27.939745610859745	21.922003873847533
98-99	23.337968186061026	29.704961099882095	28.17435308946225	18.782717624594635
100	22.25765787791465	30.623959088428137	22.090722718676105	25.02766031498111
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	642.0
1	597.5
2	787.0
3	1161.5
4	1521.0
5	2041.5
6	2461.0
7	3047.0
8	3914.5
9	4571.0
10	4835.5
11	5091.5
12	5695.0
13	6720.0
14	8626.5
15	10763.5
16	12151.0
17	12894.5
18	12849.5
19	12231.5
20	11701.0
21	11771.5
22	13134.5
23	16059.5
24	20368.0
25	26758.5
26	35295.5
27	44290.0
28	53503.0
29	63335.5
30	72247.0
31	80985.5
32	90299.0
33	99189.0
34	108335.0
35	116781.0
36	125821.5
37	134260.5
38	140040.0
39	145567.0
40	152010.5
41	157081.5
42	160610.5
43	169691.0
44	175835.5
45	175755.5
46	174390.5
47	170241.0
48	165862.0
49	161802.0
50	158326.0
51	152665.0
52	145153.0
53	137239.0
54	128681.0
55	118907.0
56	108309.5
57	97341.5
58	85908.5
59	74318.0
60	62457.0
61	50729.0
62	39990.0
63	30796.0
64	22978.0
65	16842.0
66	12419.0
67	9456.0
68	7160.5
69	5253.5
70	3808.0
71	2693.5
72	1849.5
73	1257.5
74	862.0
75	562.0
76	356.5
77	209.5
78	134.0
79	85.0
80	54.5
81	36.0
82	22.5
83	9.5
84	5.5
85	3.0
86	4.0
87	5.0
88	3.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0072456257050647925
3	0.0
4	0.003924713923576763
5	0.0031448028233788164
6	0.0
7	0.0
8	0.0
9	3.019010710443664E-4
10-11	4.27693183979519E-4
12-13	2.515842258703053E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.560525295514579E-4
24-25	0.0016354264569479646
26-27	0.0
28-29	0.0021521133879324078
30-31	0.0030863785391628683
32-33	7.448072104407328E-4
34-35	3.7977947724874075E-5
36-37	0.0013220207341155712
38-39	0.0022762742148260645
40-41	0.0016347049666455829
42-43	0.0
44-45	0.0
46-47	0.001647056591283302
48-49	5.981930581689576E-4
50-51	0.0017425149933362205
52-53	0.0026208491794512995
54-55	0.0019338798314310493
56-57	0.0020333561078376672
58-59	0.0019685317719641705
60-61	8.672360864209153E-4
62-63	8.328569109478334E-4
64-65	0.003676013810413435
66-67	0.0025768268694806957
68-69	1.0191557604867487E-4
70-71	0.0031375266303096563
72-73	0.010339846740493869
74-75	0.012387689469861143
76-77	0.005175864456897304
78-79	0.004553642290783598
80-81	2.954572667714763E-4
82-83	4.715098799394393E-5
84-85	9.529000560305233E-5
86-87	0.0018939333142869983
88-89	0.004673152721389393
90-91	8.370070008250263E-4
92-93	0.0010125926688290777
94-95	0.002099270646200849
96-97	0.0022015830918418544
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	24.0
20-21	77.0
22-23	158.0
24-25	309.0
26-27	921.0
28-29	2402.0
30-31	7857.0
32-33	10260.0
34-35	14268.0
36-37	22185.0
38-39	25616.0
40-41	25143.0
42-43	28573.0
44-45	33704.0
46-47	33889.0
48-49	31757.0
50-51	29458.0
52-53	29079.0
54-55	31710.0
56-57	32516.0
58-59	32253.0
60-61	32708.0
62-63	32450.0
64-65	34592.0
66-67	38395.0
68-69	39502.0
70-71	45013.0
72-73	34150.0
74-75	32922.0
76-77	33980.0
78-79	34959.0
80-81	34403.0
82-83	33106.0
84-85	33029.0
86-87	33423.0
88-89	34754.0
90-91	34812.0
92-93	34206.0
94-95	39014.0
96-97	119418.0
98-99	259155.0
100-101	2568662.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.11723563667138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.88543538190895	81.88575996507721
2	6.1221600112251116	10.9117995265151
3	1.2317495459673886	3.293103436000163
4	0.3938003008685424	1.403775768251759
5	0.14757263189348807	0.6575632504987873
6	0.07233054563425795	0.38675389674103067
7	0.04560924465348473	0.28451988620966356
8	0.02741680519300264	0.19546479110316256
9	0.019679550813447733	0.15784084503592824
>10	0.05316229161724781	0.6772512077306735
>50	6.633311659703369E-4	0.04385268782724662
>100	3.823391368820667E-4	0.07910536098223934
>500	3.801992223725316E-5	0.023209378027000703
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.5158422587030532E-5	0.0	0.0	0.0
3	0.0	2.5158422587030532E-5	0.0	0.0	0.0
4	0.0	5.0316845174061065E-5	0.0	0.0	0.0
5	2.5158422587030532E-5	1.0063369034812213E-4	0.0	0.0	0.0
6	2.5158422587030532E-5	1.0063369034812213E-4	0.0	0.0	2.5158422587030532E-5
7	2.5158422587030532E-5	1.0063369034812213E-4	0.0	0.0	7.54752677610916E-5
8	2.5158422587030532E-5	1.2579211293515266E-4	0.0	2.5158422587030532E-5	7.54752677610916E-5
9	2.5158422587030532E-5	2.515842258703053E-4	0.0	1.0063369034812213E-4	1.0063369034812213E-4
10-11	7.54752677610916E-5	2.641634371638206E-4	0.0	1.132129016416374E-4	1.0063369034812213E-4
12-13	7.54752677610916E-5	2.7674264845733586E-4	0.0	1.3837132422866793E-4	1.132129016416374E-4
14-15	7.54752677610916E-5	3.270594936313969E-4	0.0	1.6352974681569846E-4	1.88688169402729E-4
16-17	7.54752677610916E-5	3.270594936313969E-4	0.0	2.0126738069624426E-4	2.264258032832748E-4
18-19	7.54752677610916E-5	3.8995555009897325E-4	0.0	2.0126738069624426E-4	2.7674264845733586E-4
20-21	7.54752677610916E-5	4.528516065665496E-4	0.0	2.264258032832748E-4	3.019010710443664E-4
22-23	7.54752677610916E-5	5.283268743276412E-4	0.0	2.3900501457679006E-4	3.396387049249122E-4
24-25	7.54752677610916E-5	5.534852969146717E-4	0.0	3.019010710443664E-4	3.77376338805458E-4
26-27	7.54752677610916E-5	6.038021420887328E-4	0.0	3.8995555009897325E-4	4.025347613924885E-4
28-29	7.54752677610916E-5	6.16381353382248E-4	0.0	5.786437195017022E-4	4.025347613924885E-4
30-31	7.54752677610916E-5	7.295942550238854E-4	0.0	0.001610139045569954	4.151139726860038E-4
32-33	7.54752677610916E-5	7.295942550238854E-4	0.0	0.0038743970784027018	4.2769318397951905E-4
34-35	7.54752677610916E-5	8.679655792525533E-4	0.0	0.006817932521085274	4.402723952730343E-4
36-37	7.54752677610916E-5	0.0010063369034812213	0.0	0.010868438557597188	4.528516065665496E-4
38-39	7.54752677610916E-5	0.0010818121712423128	0.0	0.016516504428385544	4.780100291535801E-4
40-41	7.54752677610916E-5	0.001232762706764496	0.0	0.02278095165255615	4.780100291535801E-4
42-43	7.54752677610916E-5	0.0012956587632320725	0.0	0.028617705692747228	4.905892404470954E-4
44-45	7.54752677610916E-5	0.0015220845665153472	0.0	0.03557400953806117	5.157476630341259E-4
46-47	7.54752677610916E-5	0.0016604558907440151	0.0	0.043637283977204455	5.409060856211565E-4
48-49	7.54752677610916E-5	0.0016604558907440151	0.0	0.0514238157678904	5.786437195017022E-4
50-51	7.54752677610916E-5	0.0019120401166143205	0.0	0.05962546153126236	6.038021420887328E-4
52-53	7.54752677610916E-5	0.0019120401166143205	0.0	0.06737425568806776	6.038021420887328E-4
54-55	7.54752677610916E-5	0.0019246193279078357	0.0	0.07568911435308134	6.038021420887328E-4
56-57	7.54752677610916E-5	0.0019623569617883813	0.0	0.08368949273575707	6.415397759692786E-4
58-59	7.54752677610916E-5	0.0020126738069624426	0.0	0.09141312846997543	6.792774098498244E-4
60-61	7.54752677610916E-5	0.0021007282860170495	0.0	0.0985581204846921	6.792774098498244E-4
62-63	7.54752677610916E-5	0.002163624342484626	0.0	0.10535089458319036	6.792774098498244E-4
64-65	7.54752677610916E-5	0.0022642580328327477	0.0	0.11146439127183877	6.792774098498244E-4
66-67	7.54752677610916E-5	0.0022894164554197786	0.0	0.1180810564122278	6.792774098498244E-4
68-69	7.54752677610916E-5	0.002327154089300324	0.0	0.1242574491573438	6.792774098498244E-4
70-71	7.54752677610916E-5	0.0023523125118873546	0.0	0.12928913367474992	7.044358324368549E-4
72-73	7.54752677610916E-5	0.002377470934474385	0.0	0.13326416444350073	7.54752677610916E-4
74-75	7.54752677610916E-5	0.0024529462022354767	0.0	0.1368743980847396	7.54752677610916E-4
76-77	7.54752677610916E-5	0.002528421469996568	0.0	0.14004435933070544	7.54752677610916E-4
78-79	7.54752677610916E-5	0.002566159103877114	0.0	0.14224572130707064	7.54752677610916E-4
80-81	7.54752677610916E-5	0.0025913175264641445	0.0	0.14291241950562694	7.924903114914618E-4
82-83	7.54752677610916E-5	0.0026164759490511754	0.0	0.14346590480254162	9.560200583071602E-4
84-85	7.54752677610916E-5	0.0026667927942252362	0.0	0.14374264745099893	9.93757692187706E-4
86-87	7.54752677610916E-5	0.0027171096393992975	0.0	0.14385586035264059	0.001044074537361767
88	7.54752677610916E-5	0.00284290175233445	0.0	0.14385586035264059	0.0010566537486552824
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4685	0.0	48.252113	1
GTATCAA	8270	0.0	37.919724	1
CTTATAC	3745	0.0	32.791428	1
TCAACGC	9875	0.0	31.194424	4
ATCAACG	9880	0.0	31.092627	3
CAACGCA	10165	0.0	30.21897	5
TATCAAC	10490	0.0	29.81375	2
AACGCAG	10605	0.0	29.043852	6
TATACAC	5160	0.0	27.667225	3
ACGCAGA	12305	0.0	24.996767	7
CGCAGAG	12525	0.0	24.557701	8
TTATACA	5560	0.0	23.921247	2
ACACATC	5840	0.0	22.333275	6
ACATCTC	5930	0.0	21.636106	8
TACACAT	6490	0.0	21.603195	5
GTGGTAT	2165	0.0	20.608467	1
GCAGAGT	14875	0.0	20.563755	9
TATGCCG	3580	0.0	19.741209	42-43
CACATCT	6520	0.0	19.22212	7
GAGTACT	8545	0.0	19.116623	12-13
>>END_MODULE