FastQCFastQC Report
Fri 27 May 2016
SRR2140060_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140060_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4309485
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48800.049.7620431
GTATCAA93050.040.1821171
TCAACGC106550.034.327284
ATCAACG107500.034.0239223
CAACGCA109300.033.3460465
AACGCAG113050.032.2384226
TATCAAC115150.032.1726722
ACGCAGA128450.028.3733257
CGCAGAG131500.027.7478078
GCAGAGT158350.023.0699029
GTACATG122350.021.6681391
TGGTATC22300.021.1262682
TACATGG119650.021.1190432
GAGTACT90200.020.96230112-13
ACATGGG122450.020.1814333
GTGGTAT26400.019.9542851
CAGAGTA151150.019.90457710-11
AGAGTAC141050.019.20446610-11
CATGGGG88850.018.9921324
GTACTTT105800.017.8309714-15
Produced by FastQC (version 0.11.5)