FastQCFastQC Report
Fri 27 May 2016
SRR2140059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7944010
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82850.048.976791
GTATCAA143200.034.847351
TCAACGC163200.029.6512624
ATCAACG163550.029.5350173
CAACGCA167250.028.9332495
AACGCAG174800.027.7075566
TATCAAC176800.027.5899642
ACGCAGA200100.024.2471397
CGCAGAG204650.023.6447738
GTACATG195300.020.5337581
TACATGG192950.020.045562
ACATGGG196050.019.3984383
GCAGAGT251850.019.0934569
GAGTACT139600.019.07824112-13
CTTATAC63000.018.0206381
TGGTATC41550.017.86952
CAGAGTA245850.017.82987410-11
AGAGTAC221600.017.66754210-11
CATGGGG151400.017.5350484
TATGCCG33900.017.18474242-43
Produced by FastQC (version 0.11.5)