##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140059_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7944010
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.877141770969573	32.0	18.0	33.0	18.0	33.0
2	31.235004361776987	32.0	32.0	33.0	27.0	34.0
3	32.443930206532976	33.0	33.0	33.0	31.0	34.0
4	32.76450646461925	33.0	33.0	34.0	31.0	34.0
5	33.1512294168814	33.0	33.0	34.0	33.0	34.0
6	37.038934744543376	38.0	37.0	38.0	36.0	38.0
7	37.35433049555577	38.0	38.0	38.0	37.0	38.0
8	37.29588847446063	38.0	38.0	38.0	36.0	38.0
9	37.45351252075463	38.0	38.0	38.0	37.0	38.0
10-11	37.487852923145866	38.0	38.0	38.0	37.0	38.0
12-13	37.50801226332797	38.0	38.0	38.0	37.0	38.0
14-15	37.45978479382579	38.0	38.0	38.0	37.0	38.0
16-17	37.47609425214722	38.0	38.0	38.0	37.0	38.0
18-19	37.39088590019398	38.0	38.0	38.0	37.0	38.0
20-21	37.48672706824179	38.0	38.0	38.0	37.0	38.0
22-23	37.50359127714276	38.0	38.0	38.0	37.0	38.0
24-25	37.51149349881208	38.0	38.0	38.0	37.0	38.0
26-27	37.49674957076232	38.0	38.0	38.0	37.0	38.0
28-29	37.430355606297375	38.0	38.0	38.0	37.0	38.0
30-31	37.47682542904285	38.0	38.0	38.0	37.0	38.0
32-33	37.473018247293794	38.0	38.0	38.0	37.0	38.0
34-35	37.45716444671655	38.0	38.0	38.0	37.0	38.0
36-37	37.44042058474459	38.0	38.0	38.0	37.0	38.0
38-39	37.43046260628262	38.0	38.0	38.0	37.0	38.0
40-41	37.418772705603544	38.0	38.0	38.0	37.0	38.0
42-43	37.35492875206796	38.0	38.0	38.0	37.0	38.0
44-45	37.37218686523226	38.0	38.0	38.0	37.0	38.0
46-47	37.36447919952903	38.0	38.0	38.0	37.0	38.0
48-49	37.33639564912408	38.0	38.0	38.0	37.0	38.0
50-51	37.30415249033008	38.0	38.0	38.0	37.0	38.0
52-53	37.28245101926143	38.0	38.0	38.0	37.0	38.0
54-55	37.267008598502564	38.0	38.0	38.0	37.0	38.0
56-57	37.26557920728607	38.0	38.0	38.0	37.0	38.0
58-59	37.25745578299231	38.0	38.0	38.0	37.0	38.0
60-61	37.25182060936125	38.0	38.0	38.0	37.0	38.0
62-63	37.25075641427117	38.0	38.0	38.0	37.0	38.0
64-65	37.24861610184632	38.0	38.0	38.0	37.0	38.0
66-67	37.24780673108596	38.0	38.0	38.0	37.0	38.0
68-69	37.24507034907466	38.0	38.0	38.0	37.0	38.0
70-71	37.247760343118344	38.0	38.0	38.0	37.0	38.0
72-73	37.21829427845926	38.0	38.0	38.0	37.0	38.0
74-75	37.213579215403755	38.0	38.0	38.0	36.0	38.0
76-77	37.21047910016166	38.0	38.0	38.0	36.0	38.0
78-79	37.19359754378939	38.0	38.0	38.0	36.0	38.0
80-81	37.18216450575366	38.0	38.0	38.0	36.0	38.0
82-83	37.1797949536518	38.0	38.0	38.0	36.0	38.0
84-85	37.15783210692818	38.0	38.0	38.0	36.0	38.0
86-87	37.13831739681579	38.0	38.0	38.0	36.0	38.0
88-89	37.116401068179236	38.0	38.0	38.0	36.0	38.0
90-91	37.101492101483984	38.0	38.0	38.0	36.0	38.0
92-93	37.09422473918332	38.0	38.0	38.0	36.0	38.0
94-95	37.0509435850677	38.0	38.0	38.0	36.0	38.0
96-97	37.05942659588028	38.0	38.0	38.0	36.0	38.0
98-99	37.05082182634885	38.0	38.0	38.0	36.0	38.0
100	35.37762792785327	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	8.0
19	23.0
20	99.0
21	312.0
22	683.0
23	1531.0
24	2934.0
25	5755.0
26	9231.0
27	14688.0
28	22293.0
29	32491.0
30	44925.0
31	60529.0
32	80771.0
33	110032.0
34	166029.0
35	309089.0
36	1009212.0
37	6073374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44436877597083	19.007327533575612	14.101178120369939	23.447125570083625
2	14.322869067778049	20.596934436456852	42.65937964659564	22.42081684916946
3	18.064705356614606	26.04163388515372	30.352429062904	25.541231695327678
4	12.398801109263458	18.33081781115583	39.16146882997378	30.108912249606934
5	13.584549469900164	37.430305511431975	35.75237568668505	13.23276933198281
6	29.86212218293326	37.90917410520406	19.11859153901251	13.110112172850167
7	26.59157025230331	32.60222482096574	23.240794007056888	17.56541091967407
8	24.09449990483393	36.948930565024604	21.47662741527954	17.479942114861917
9	25.388702263016683	17.337177073810945	21.58488710596352	35.68923355720885
10-11	23.987734154413197	26.90459226511548	29.22407575015641	19.883597830314915
12-13	24.780539047659808	24.357106801225072	29.237079258460145	21.625274892654968
14-15	22.03924227432004	25.878040553702764	26.738575778389993	25.344141393587204
16-17	21.7863648207895	28.99252392683292	27.467928665749415	21.753182586628164
18-19	21.830788229118543	28.228860487335737	28.942983455458894	20.997367828086823
20-21	22.493685749734137	27.37385472390513	28.881891797718414	21.250567728642324
22-23	22.26159203526037	27.363110364893213	28.67556778038115	21.699729819465272
24-25	22.089213969041126	27.433204524229115	28.79204185457666	21.685539652153103
26-27	22.070510965097544	27.611049412970978	28.702460205119284	21.6159794168122
28-29	21.959560863184656	27.55005684811532	28.73907876774367	21.751303520956355
30-31	22.207009353803222	27.364644327631122	28.734067130021696	21.69427918854396
32-33	21.999784023290143	27.53888606382825	28.693219094991946	21.768110817889664
34-35	22.186682717636085	27.630602777280718	28.676142279547594	21.506572225535603
36-37	22.140047753187282	27.513614075500097	28.615661476586634	21.73067669472599
38-39	22.002537261281987	27.604275246358785	28.749065649993522	21.644121842365706
40-41	22.274361065887263	27.429445883145455	28.635020771913595	21.66117227905369
42-43	22.221472397936775	27.476073407840516	28.515529677379188	21.786924516843527
44-45	22.191402604707104	27.594844819437963	28.448395485243548	21.765357090611385
46-47	22.312081510099564	27.524217898549942	28.241393907496715	21.92230668385378
48-49	22.22206498414316	27.610809952652616	28.271780329832247	21.895344733371974
50-51	22.137700232362757	27.71720310821718	28.270410838903043	21.87468582051702
52-53	22.330542361824897	27.678922439984554	28.212072924997283	21.778462273193266
54-55	22.3212313521168	27.564801062614297	28.168376079737968	21.94559150553094
56-57	22.36148697055772	27.7021528398137	28.06494337331499	21.87141681631359
58-59	22.291386941102875	27.79992801607732	28.17335483224095	21.73533021057886
60-61	22.44494671253094	27.848601512307752	28.059744878097288	21.646706897064025
62-63	22.34512836885542	27.959593576862922	28.002526766873608	21.69275128740805
64-65	22.410065491659815	27.935611642162854	27.945393379825852	21.70892948635148
66-67	22.48608144085329	27.929543371219467	27.81812886006073	21.76624632786651
68-69	22.344860358888088	27.89447468458378	27.88568511406134	21.874979842466796
70-71	22.3716632494944	27.688688393162643	27.911237981624744	22.028410375718217
72-73	22.354491507231327	27.61134326133065	27.975704981131837	22.058460250306187
74-75	22.287799464041733	27.696191724008813	27.950125356828952	22.06588345512051
76-77	22.34347633450379	27.652287034711932	27.86472853117664	22.139508099607635
78-79	22.30026643651947	27.638512945705607	27.866840483576127	22.1943801341988
80-81	22.288249590038774	27.66675818093786	27.856452516034775	22.188539712988586
82-83	22.267229851453667	27.646616357265664	27.970594209522154	22.11555958175852
84-85	22.38385679646466	27.64163214316571	27.875520330870458	22.09899072949917
86-87	22.262028798198106	27.617105773470623	27.962018846340314	22.158846581990954
88-89	22.288401887383426	27.618488025871855	27.896007380412218	22.197102706332497
90-91	22.311540406453176	27.65053571937479	27.90941984127165	22.128504032900384
92-93	22.32617870754245	27.645268504454844	27.935403847286956	22.09314894071575
94-95	22.301483074261423	27.716656754607904	27.879750926165087	22.102109244965586
96-97	22.361263525264516	27.807769737253185	27.96756051872449	21.86340621875781
98-99	23.447949056242756	29.69835646538766	28.209834992927114	18.64385948544247
100	22.315581790416143	30.609022401661452	22.142699423286672	24.932696384635737
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	705.0
1	707.0
2	1137.5
3	1809.0
4	2394.0
5	3133.5
6	3748.5
7	4684.0
8	6065.0
9	7154.0
10	7766.0
11	8357.5
12	9302.5
13	10931.0
14	13713.0
15	16697.5
16	18928.0
17	20323.0
18	20565.5
19	19849.0
20	19135.5
21	19511.5
22	21752.5
23	26474.5
24	34362.0
25	45521.0
26	60213.5
27	76806.0
28	95003.5
29	115010.0
30	133537.0
31	152070.5
32	172283.0
33	191339.5
34	209431.0
35	227031.0
36	245896.0
37	263612.5
38	277951.5
39	291330.5
40	305618.0
41	316954.0
42	324104.5
43	334879.0
44	340882.0
45	341548.5
46	340958.0
47	337646.0
48	334616.0
49	326725.0
50	316517.0
51	305256.5
52	290351.5
53	272999.0
54	254588.0
55	234190.5
56	213013.5
57	192563.5
58	170828.0
59	147637.5
60	122213.0
61	98286.5
62	77867.5
63	59711.5
64	44768.0
65	33334.5
66	24803.0
67	18916.5
68	14383.5
69	10587.0
70	7802.0
71	5626.0
72	3992.0
73	2697.0
74	1757.0
75	1139.5
76	702.0
77	418.0
78	278.5
79	183.5
80	112.5
81	74.5
82	50.5
83	27.5
84	17.0
85	12.5
86	9.5
87	7.5
88	5.5
89	4.0
90	3.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.769382213768613E-4
3	0.0
4	0.0
5	0.005186297600330312
6	2.517620194335103E-4
7	0.0
8	2.517620194335103E-5
9	1.5105721166010615E-4
10-11	0.0
12-13	0.0
14-15	6.294050485837757E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.3847940424146048E-4
26-27	1.8885041026807378E-5
28-29	6.800193503284022E-4
30-31	2.58342949256971E-4
32-33	0.001476849904399214
34-35	3.478878652411445E-4
36-37	0.0013010042038936326
38-39	8.099069346782634E-4
40-41	7.564555467615817E-4
42-43	1.4180038012814628E-4
44-45	5.189819934656275E-5
46-47	4.7049567372693343E-4
48-49	0.0014809413705842525
50-51	0.0015839199912307826
52-53	0.0016207960269553718
54-55	0.0012151918858482312
56-57	0.0028602103627247107
58-59	4.15499719810148E-4
60-61	1.3724419825885147E-5
62-63	0.0019081053688969145
64-65	0.002515041655987029
66-67	0.0030629446362921845
68-69	7.442726096196597E-4
70-71	0.00455665792818421
72-73	0.001170127426876787
74-75	0.004589346378732532
76-77	2.866055976277875E-4
78-79	8.38123555581202E-4
80-81	0.0
82-83	2.267005659579629E-5
84-85	8.693235176032293E-4
86-87	0.0
88-89	3.108850009956092E-5
90-91	0.0027948497827122057
92-93	8.088767723025368E-4
94-95	8.014061151133333E-6
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	46.0
20-21	139.0
22-23	315.0
24-25	493.0
26-27	1347.0
28-29	3614.0
30-31	12242.0
32-33	16013.0
34-35	22771.0
36-37	36499.0
38-39	40925.0
40-41	41173.0
42-43	47392.0
44-45	55324.0
46-47	55824.0
48-49	52721.0
50-51	49212.0
52-53	47764.0
54-55	52194.0
56-57	54055.0
58-59	54012.0
60-61	54205.0
62-63	54635.0
64-65	58161.0
66-67	62676.0
68-69	64074.0
70-71	68672.0
72-73	59167.0
74-75	59268.0
76-77	62068.0
78-79	62862.0
80-81	62380.0
82-83	60042.0
84-85	59976.0
86-87	62595.0
88-89	64123.0
90-91	64157.0
92-93	64204.0
94-95	76197.0
96-97	245633.0
98-99	540999.0
100-101	5393841.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.72687893750302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.01826233964961	77.16984677755528
2	6.971479274859835	11.952863196224408
3	1.7124440037865971	4.404074393995997
4	0.6187757877898892	2.1218286819728758
5	0.273065554470629	1.1704528865052874
6	0.13830838520797717	0.7114047716859475
7	0.08056090118015796	0.48343642357972816
8	0.05225523231874937	0.3583742379872418
9	0.03437244540932825	0.26519782197522734
>10	0.09924953334438298	1.2625716853759585
>50	9.873213067422362E-4	0.05444206801496931
>100	2.16571510232855E-4	0.0348086060654163
>500	2.2649165839216067E-5	0.010698449061615815
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.7764302915026544E-5	0.0	0.0	0.0
3	0.0	3.7764302915026544E-5	0.0	0.0	0.0
4	0.0	7.552860583005309E-5	0.0	0.0	0.0
5	0.0	1.0070480777340412E-4	0.0	0.0	0.0
6	7.552860583005309E-5	1.0070480777340412E-4	0.0	1.2588100971675515E-5	0.0
7	7.552860583005309E-5	1.0070480777340412E-4	0.0	1.2588100971675515E-5	0.0
8	7.552860583005309E-5	1.2588100971675515E-4	0.0	1.2588100971675515E-5	0.0
9	7.552860583005309E-5	2.0140961554680824E-4	0.0	2.517620194335103E-5	1.2588100971675515E-5
10-11	8.182265631589085E-5	2.0140961554680824E-4	0.0	5.035240388670206E-5	1.2588100971675515E-5
12-13	1.0070480777340412E-4	2.0770366603264597E-4	0.0	6.294050485837757E-5	1.2588100971675515E-5
14-15	1.1329290874507963E-4	3.3358467574940115E-4	0.0	6.294050485837757E-5	1.2588100971675515E-5
16-17	1.2588100971675515E-4	3.524668272069144E-4	0.0	6.294050485837757E-5	1.2588100971675515E-5
18-19	1.3846911068843065E-4	3.5876087769275215E-4	0.0	6.294050485837757E-5	1.2588100971675515E-5
20-21	1.5735126214594391E-4	3.9652518060777873E-4	0.0	8.182265631589085E-5	1.2588100971675515E-5
22-23	1.762334136034572E-4	4.3428948352280527E-4	0.0	1.3846911068843065E-4	1.2588100971675515E-5
24-25	1.762334136034572E-4	4.8464188740950727E-4	0.0	1.5735126214594391E-4	1.2588100971675515E-5
26-27	1.762334136034572E-4	5.224061903245338E-4	0.0	1.888215145751327E-4	1.2588100971675515E-5
28-29	1.762334136034572E-4	5.349942912962094E-4	0.0	3.2099657477772557E-4	1.2588100971675515E-5
30-31	1.762334136034572E-4	6.168169476121002E-4	0.0	9.944599767623655E-4	1.2588100971675515E-5
32-33	1.762334136034572E-4	6.168169476121002E-4	0.0	0.0025994428506509936	1.2588100971675515E-5
34-35	1.888215145751327E-4	7.049336544138287E-4	0.0	0.005041534439156043	1.2588100971675515E-5
36-37	1.888215145751327E-4	8.056384621872329E-4	0.0	0.008396263348107568	1.2588100971675515E-5
38-39	1.888215145751327E-4	8.685789670456105E-4	0.0	0.01644635391949406	1.2588100971675515E-5
40-41	2.0140961554680824E-4	9.566956738473391E-4	0.0	0.024603443349139793	1.8882151457513272E-5
42-43	2.1399771651848374E-4	0.001007048077734041	0.0	0.030607967512629012	2.517620194335103E-5
44-45	2.202917670043215E-4	0.0010951647845357697	0.0	0.03757548140045141	2.517620194335103E-5
46-47	2.3917391846183477E-4	0.001164399339879985	0.0	0.04523534084171596	3.7764302915026544E-5
48-49	2.517620194335103E-4	0.0011706933903658228	0.0	0.05263085016257532	3.7764302915026544E-5
50-51	2.6435012040518577E-4	0.001296574400082578	0.0	0.06056764782521674	4.40583534008643E-5
52-53	2.6435012040518577E-4	0.0013091625010542534	0.0	0.07713988275442754	5.035240388670206E-5
54-55	2.7064417089102356E-4	0.0013091625010542534	0.0	0.09650667609935032	5.035240388670206E-5
56-57	2.769382213768613E-4	0.001321750602025929	0.0	0.12103459084266006	5.6646454372539816E-5
58-59	2.769382213768613E-4	0.00134692680396928	0.0	0.1426105959081119	6.294050485837757E-5
60-61	2.895263223485368E-4	0.0013595149049409555	0.0	0.15174326316306247	6.294050485837757E-5
62-63	3.2729062526356336E-4	0.001397279207855982	0.0	0.1638026638939276	6.294050485837757E-5
64-65	3.2729062526356336E-4	0.0015357483185444126	0.0	0.16987642261276104	6.294050485837757E-5
66-67	3.2729062526356336E-4	0.0015483364195160883	0.0	0.17526412982863818	6.294050485837757E-5
68-69	3.398787262352389E-4	0.001586100722431115	0.0	0.1807210715998595	6.294050485837757E-5
70-71	3.5246682720691436E-4	0.0016427471768036546	0.0	0.1853220225050069	6.294050485837757E-5
72-73	3.776430291502654E-4	0.0016679233787470055	0.0	0.18919286355379714	7.552860583005309E-5
74-75	3.8393707963610315E-4	0.0016993936311761944	0.0	0.1920377743733958	7.552860583005309E-5
76-77	3.9023113012194094E-4	0.0017749222370062475	0.0	0.1951533293638855	7.552860583005309E-5
78-79	3.9023113012194094E-4	0.0018000984389495984	0.0	0.19706672071158016	7.552860583005309E-5
80-81	3.9023113012194094E-4	0.0018000984389495984	0.0	0.19769612576016393	7.552860583005309E-5
82-83	3.9023113012194094E-4	0.0018063924894354362	0.0	0.19806747473882838	8.81167068017286E-5
84-85	3.9652518060777873E-4	0.0018252746408929496	0.0	0.19823112005146015	8.81167068017286E-5
86-87	4.279954330369675E-4	0.001837862741864625	0.0	0.1983192367582619	8.81167068017286E-5
88	4.40583534008643E-4	0.0019511556506097048	0.0	0.19832553080874774	8.81167068017286E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8285	0.0	48.97679	1
GTATCAA	14320	0.0	34.84735	1
TCAACGC	16320	0.0	29.651262	4
ATCAACG	16355	0.0	29.535017	3
CAACGCA	16725	0.0	28.933249	5
AACGCAG	17480	0.0	27.707556	6
TATCAAC	17680	0.0	27.589964	2
ACGCAGA	20010	0.0	24.247139	7
CGCAGAG	20465	0.0	23.644773	8
GTACATG	19530	0.0	20.533758	1
TACATGG	19295	0.0	20.04556	2
ACATGGG	19605	0.0	19.398438	3
GCAGAGT	25185	0.0	19.093456	9
GAGTACT	13960	0.0	19.078241	12-13
CTTATAC	6300	0.0	18.020638	1
TGGTATC	4155	0.0	17.8695	2
CAGAGTA	24585	0.0	17.829874	10-11
AGAGTAC	22160	0.0	17.667542	10-11
CATGGGG	15140	0.0	17.535048	4
TATGCCG	3390	0.0	17.184742	42-43
>>END_MODULE