FastQCFastQC Report
Fri 27 May 2016
SRR2140058_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140058_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4990394
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60450.048.2741971
GTATCAA110300.038.4612961
ATCAACG125700.033.0674483
TCAACGC125850.033.0280384
TATCAAC133200.031.591592
CAACGCA131650.031.5729475
AACGCAG136400.030.5969646
CTTATAC36050.028.1713261
ACGCAGA154200.027.065027
CGCAGAG155300.026.8181368
TATACAC53100.022.6764983
GCAGAGT190750.021.90159
GTGGTAT29200.020.9854091
GAGTACT108300.020.51417512-13
GTACATG140550.020.030821
TACATGG138900.019.9294132
CAGAGTA185050.019.21864510-11
ACCGAAC4050.019.0436978
ACATGGG140050.018.9090463
TGGTATC28700.018.8127752
Produced by FastQC (version 0.11.5)