FastQCFastQC Report
Fri 27 May 2016
SRR2140057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4567473
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52300.050.3298071
GTATCAA95750.037.309011
CTTATAC38250.036.977951
TCAACGC114150.030.6961674
ATCAACG114950.030.5568853
CAACGCA117500.029.7118995
TATCAAC120700.029.3490122
AACGCAG121400.028.6866876
TATACAC56650.028.6635463
ACGCAGA138250.025.1903367
CGCAGAG141250.024.6553218
TTATACA63100.024.1760372
ACATCTC68600.021.9883238
ACACATC68800.021.613866
TACACAT76700.021.059245
GCAGAGT168700.020.5675459
TATGCCG42000.020.2163642-43
GTGGTAT26250.020.1854571
CTCGTAT41400.019.90091338-39
GTACATG126900.019.8335251
Produced by FastQC (version 0.11.5)