##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140057_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4567473
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.545143890286816	32.0	18.0	33.0	18.0	33.0
2	31.09951585920705	32.0	31.0	33.0	27.0	33.0
3	32.37589625598225	33.0	33.0	33.0	30.0	34.0
4	32.69975454698911	33.0	33.0	34.0	31.0	34.0
5	33.1009418118071	33.0	33.0	34.0	33.0	34.0
6	37.00562214598751	38.0	37.0	38.0	35.0	38.0
7	37.347919735924	38.0	38.0	38.0	37.0	38.0
8	37.29970051273428	38.0	38.0	38.0	36.0	38.0
9	37.45430131716159	38.0	38.0	38.0	37.0	38.0
10-11	37.49156995564068	38.0	38.0	38.0	37.0	38.0
12-13	37.50976458974142	38.0	38.0	38.0	37.0	38.0
14-15	37.46335271166354	38.0	38.0	38.0	37.0	38.0
16-17	37.4814795840063	38.0	38.0	38.0	37.0	38.0
18-19	37.40522253771396	38.0	38.0	38.0	37.0	38.0
20-21	37.494741924991864	38.0	38.0	38.0	37.0	38.0
22-23	37.50954354443925	38.0	38.0	38.0	37.0	38.0
24-25	37.51455485396343	38.0	38.0	38.0	37.0	38.0
26-27	37.49733384304551	38.0	38.0	38.0	37.0	38.0
28-29	37.43604589778678	38.0	38.0	38.0	37.0	38.0
30-31	37.47777446138826	38.0	38.0	38.0	37.0	38.0
32-33	37.47280477254732	38.0	38.0	38.0	37.0	38.0
34-35	37.45196403523278	38.0	38.0	38.0	37.0	38.0
36-37	37.432615415030426	38.0	38.0	38.0	37.0	38.0
38-39	37.413728269184375	38.0	38.0	38.0	37.0	38.0
40-41	37.40828162894847	38.0	38.0	38.0	37.0	38.0
42-43	37.343020571122835	38.0	38.0	38.0	37.0	38.0
44-45	37.35323313898725	38.0	38.0	38.0	37.0	38.0
46-47	37.33644786863597	38.0	38.0	38.0	37.0	38.0
48-49	37.30060214582006	38.0	38.0	38.0	37.0	38.0
50-51	37.26874717598556	38.0	38.0	38.0	37.0	38.0
52-53	37.24849343828732	38.0	38.0	38.0	37.0	38.0
54-55	37.23108082437946	38.0	38.0	38.0	37.0	38.0
56-57	37.2328253500674	38.0	38.0	38.0	37.0	38.0
58-59	37.22269748528615	38.0	38.0	38.0	37.0	38.0
60-61	37.21782157475491	38.0	38.0	38.0	37.0	38.0
62-63	37.218575038871144	38.0	38.0	38.0	37.0	38.0
64-65	37.21677883833014	38.0	38.0	38.0	37.0	38.0
66-67	37.21498620168775	38.0	38.0	38.0	37.0	38.0
68-69	37.2150987529198	38.0	38.0	38.0	37.0	38.0
70-71	37.220575337784375	38.0	38.0	38.0	37.0	38.0
72-73	37.19124839269488	38.0	38.0	38.0	36.0	38.0
74-75	37.18555237965213	38.0	38.0	38.0	36.0	38.0
76-77	37.18242453949601	38.0	38.0	38.0	36.0	38.0
78-79	37.1660728062122	38.0	38.0	38.0	36.0	38.0
80-81	37.15508232239267	38.0	38.0	38.0	36.0	38.0
82-83	37.154093540145624	38.0	38.0	38.0	36.0	38.0
84-85	37.13709543026921	38.0	38.0	38.0	36.0	38.0
86-87	37.11792385697389	38.0	38.0	38.0	36.0	38.0
88-89	37.09890941438094	38.0	38.0	38.0	36.0	38.0
90-91	37.08279762581891	38.0	38.0	38.0	36.0	38.0
92-93	37.075969472407834	38.0	38.0	38.0	36.0	38.0
94-95	37.03564488232871	38.0	38.0	38.0	36.0	38.0
96-97	37.04349863860937	38.0	38.0	38.0	36.0	38.0
98-99	37.03410895937884	38.0	38.0	38.0	36.0	38.0
100	35.361145289890736	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	15.0
20	52.0
21	133.0
22	347.0
23	810.0
24	1569.0
25	3068.0
26	5220.0
27	8526.0
28	13386.0
29	19984.0
30	28009.0
31	37466.0
32	49590.0
33	66300.0
34	99756.0
35	183317.0
36	598003.0
37	3451919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.179653169268875	19.103123324429067	14.238923798783265	23.478299707518797
2	14.06781271524536	20.584845588206317	43.031206389894436	22.31613530665389
3	17.664734963950526	26.4230352319543	30.80738517775584	25.104844626339336
4	12.086201713726606	18.590104418789117	39.25621454138864	30.06747932609563
5	13.181957514636913	37.71397042402162	35.93058797255223	13.173484088789241
6	29.351387435065067	38.20421297890708	19.143168726054782	13.301230859973066
7	26.02206953385384	32.936264757339565	23.28580814818172	17.75585756062488
8	23.91202398175621	36.91534398021488	21.65081690703304	17.521815130995876
9	25.26769755146615	17.087585506893536	21.599713451741753	36.045003489898555
10-11	23.73479821336656	26.965829902004895	29.428230883904515	19.871141000724034
12-13	24.631311449460128	24.443483300284424	29.345307569415297	21.579897680840148
14-15	21.865714571899446	25.933226746302253	26.88942297955817	25.31163570224013
16-17	21.522863955627106	29.088272661929253	27.618696377625003	21.77016700481864
18-19	21.555540667673352	28.13645532223179	29.274809068384204	21.033194941710658
20-21	22.29772911532029	27.310025973047846	29.15198206780316	21.240262843828702
22-23	21.98439765241168	27.43603450202969	28.992196855687208	21.587370989871424
24-25	21.888626932355184	27.488963248781644	29.101804142757125	21.52060567610604
26-27	21.881046099560617	27.626123737623953	29.033474787405577	21.459355375409853
28-29	21.716804114701553	27.50804241303713	29.146653267573225	21.62850020468809
30-31	22.00885718933086	27.42223434241222	29.122087336393875	21.44682113186304
32-33	21.619118141063915	27.431769047184236	29.24128627827882	21.707826533473032
34-35	21.75651693334772	27.47753559845254	29.079537522871746	21.686409945328002
36-37	21.731182327514663	27.49612826467353	29.248603755102547	21.524085652709264
38-39	21.61558958805178	27.497141848855165	29.288223972823978	21.599044590269077
40-41	21.93315455901341	27.348000100147	29.16898778206582	21.549857558773773
42-43	21.99093541950251	27.34874233303028	29.025807797383408	21.634514450083802
44-45	21.856452002947236	27.639352265332672	28.897737640615205	21.60645809110489
46-47	22.113582296361713	27.40802757131484	28.546310464854805	21.93207966746864
48-49	21.97193583643257	27.424563413660024	28.665770408251962	21.937730341655442
50-51	21.792808838181223	27.624954580743722	28.707916709784993	21.874319871290066
52-53	22.002258391755365	27.553314276959973	28.77769074476562	21.66673658651904
54-55	21.976002125943804	27.46031546057779	28.623403113725864	21.940279299752543
56-57	22.152523900821013	27.683131802001476	28.348141868972565	21.816202428204946
58-59	21.85106658286149	27.949161970181336	28.67781693660161	21.52195451035557
60-61	22.114072583964113	28.153169625605045	28.281269197134062	21.451488593296773
62-63	21.89787897429906	28.406507716001496	28.155467648531733	21.540145661167713
64-65	21.906117358886764	28.321317824080865	28.174698699786415	21.59786611724595
66-67	21.95448848860241	28.3330996427944	28.052609009197987	21.6598028594052
68-69	21.932394510914953	28.173055416502468	28.20372993641428	21.690820136168306
70-71	22.0014057434807	27.82888139001375	28.26005544933369	21.90965741717186
72-73	22.084328219682707	27.649555583578216	28.32375852102279	21.94235767571629
74-75	22.008977229234088	27.755038601049524	28.274762017940212	21.96122215177618
76-77	22.067688129521994	27.682490762477457	28.18204144017475	22.067779667825803
78-79	22.0611051422355	27.737950539668837	28.172676063705147	22.028268254390515
80-81	22.058896527429358	27.694297960011525	28.16777330614772	22.07903220641139
82-83	22.120400959131768	27.692099293590555	28.196775667490204	21.990724079787473
84-85	22.267882139067364	27.65522753493072	28.10150176375437	21.975388562247545
86-87	22.03907236644454	27.670129514781898	28.222205264326618	22.068592854446944
88-89	22.15784919008088	27.666933267822447	28.091193157817507	22.084024384279168
90-91	22.176195278722787	27.743880684228415	28.117978553950834	21.961945483097963
92-93	22.234275983195225	27.71076501986575	28.041811152054457	22.013147844884568
94-95	22.153800556748514	27.80793123957676	28.024573786984103	22.013694416690623
96-97	22.218142130458578	27.914232288912515	28.097442511920406	21.7701830687085
98-99	23.253046786988822	29.81972159417664	28.304702332653203	18.622529286181333
100	22.10957265957758	30.76495877706627	22.323469004982936	24.80199955837322
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	520.0
1	533.0
2	884.5
3	1380.5
4	1710.5
5	2181.0
6	2674.5
7	3456.5
8	4727.5
9	5712.5
10	6047.0
11	6242.0
12	6832.5
13	8041.5
14	10048.5
15	12108.0
16	13643.5
17	14715.5
18	14752.5
19	13926.0
20	13097.5
21	12927.0
22	14223.0
23	17402.0
24	22633.0
25	29675.0
26	38818.0
27	49406.5
28	60042.5
29	71752.5
30	82852.5
31	93087.0
32	104395.0
33	115276.0
34	125976.0
35	136421.0
36	147792.0
37	158227.5
38	164619.5
39	169662.5
40	176014.5
41	181346.5
42	184322.5
43	187652.5
44	196650.5
45	206517.5
46	202251.5
47	194685.0
48	189594.0
49	182187.0
50	176597.0
51	170033.0
52	161702.0
53	152081.0
54	141812.0
55	130911.0
56	119692.0
57	108500.0
58	97082.0
59	84371.5
60	70236.0
61	56703.0
62	44708.0
63	34404.0
64	25834.5
65	19100.0
66	14081.5
67	10691.5
68	8131.5
69	5907.5
70	4248.5
71	3005.5
72	2082.5
73	1407.5
74	937.5
75	632.5
76	403.0
77	227.5
78	148.5
79	106.5
80	68.0
81	40.0
82	29.0
83	17.0
84	6.5
85	3.0
86	2.0
87	2.0
88	2.0
89	2.5
90	3.0
91	3.0
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.40833388615543E-4
3	0.0
4	0.0
5	0.0055391679381574885
6	3.5030311071351707E-4
7	0.0
8	2.1893944419594817E-5
9	1.094697220979741E-4
10-11	0.0
12-13	0.0
14-15	1.0946972209797409E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.532689698540737E-4
26-27	4.3794941136861925E-5
28-29	6.023422791383351E-4
30-31	2.740188180779202E-4
32-33	0.0014714966163813811
34-35	3.3027680499026234E-4
36-37	0.0011824483554629833
38-39	6.222391609549371E-4
40-41	6.929766813346731E-4
42-43	1.1244969984363868E-4
44-45	9.061435741451413E-5
46-47	4.4538922793033566E-4
48-49	0.001416094338132473
50-51	0.0016589644697375299
52-53	0.0015892393538199528
54-55	0.0011891768423704886
56-57	0.0024571803677223836
58-59	2.872764884183885E-4
60-61	1.206892369217824E-5
62-63	0.0016915832415943524
64-65	0.0021727244761954095
66-67	0.0026901582358512554
68-69	6.891695131455951E-4
70-71	0.004193237030767592
72-73	8.723152413869453E-4
74-75	0.003730119144927174
76-77	2.3538365574355736E-4
78-79	0.001003852946110268
80-81	0.0
82-83	4.0435555631035256E-5
84-85	8.438944914106513E-4
86-87	0.0
88-89	0.0
90-91	0.002668745478934468
92-93	5.253771355870592E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	27.0
20-21	87.0
22-23	171.0
24-25	305.0
26-27	911.0
28-29	2386.0
30-31	7985.0
32-33	10659.0
34-35	15012.0
36-37	23230.0
38-39	26804.0
40-41	26336.0
42-43	30460.0
44-45	35715.0
46-47	35851.0
48-49	33645.0
50-51	31452.0
52-53	31424.0
54-55	34103.0
56-57	35165.0
58-59	34235.0
60-61	34501.0
62-63	34679.0
64-65	38260.0
66-67	42117.0
68-69	43691.0
70-71	50280.0
72-73	38668.0
74-75	36420.0
76-77	38019.0
78-79	38210.0
80-81	37943.0
82-83	36310.0
84-85	36548.0
86-87	38008.0
88-89	38311.0
90-91	38814.0
92-93	38197.0
94-95	45247.0
96-97	137958.0
98-99	302265.0
100-101	3007064.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.27870345141554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.4863726781524	80.76298363500301
2	6.236850930773354	11.011622275768518
3	1.3762913173370237	3.6449163919785943
4	0.4565545991765509	1.6121619228034652
5	0.18733422060673838	0.8268811053622158
6	0.09359997813791583	0.49577308278576293
7	0.05083767256033164	0.31415186740795775
8	0.0332797648113573	0.23503155909717321
9	0.020017332019135817	0.15903937054852346
>10	0.057231140010728966	0.7557326962698319
>50	0.0011078394763716724	0.06646806835947514
>100	4.880449049582067E-4	0.08711119503505341
>500	3.4482033110557955E-5	0.02812682958040806
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.3787888839189634E-5	0.0	2.1893944419594817E-5	0.0
3	0.0	4.3787888839189634E-5	0.0	2.1893944419594817E-5	0.0
4	0.0	8.757577767837927E-5	0.0	2.1893944419594817E-5	0.0
5	0.0	8.757577767837927E-5	0.0	2.1893944419594817E-5	0.0
6	0.0	8.757577767837927E-5	0.0	2.1893944419594817E-5	0.0
7	0.0	8.757577767837927E-5	0.0	2.1893944419594817E-5	0.0
8	0.0	8.757577767837927E-5	0.0	2.1893944419594817E-5	2.1893944419594817E-5
9	0.0	1.5325761093716372E-4	0.0	2.1893944419594817E-5	2.1893944419594817E-5
10-11	0.0	1.5325761093716372E-4	0.0	2.1893944419594817E-5	2.1893944419594817E-5
12-13	0.0	1.5325761093716372E-4	0.0	2.1893944419594817E-5	2.1893944419594817E-5
14-15	0.0	1.5325761093716372E-4	0.0	2.1893944419594817E-5	2.1893944419594817E-5
16-17	0.0	1.6420458314696113E-4	0.0	2.1893944419594817E-5	5.473486104898704E-5
18-19	0.0	1.8609852756655594E-4	0.0	2.1893944419594817E-5	6.568183325878445E-5
20-21	0.0	1.9704549977635335E-4	0.0	2.1893944419594817E-5	6.568183325878445E-5
22-23	0.0	2.40833388615543E-4	0.0	2.1893944419594817E-5	6.568183325878445E-5
24-25	0.0	2.9556824966453E-4	0.0	4.3787888839189634E-5	8.757577767837927E-5
26-27	0.0	3.2840916629392226E-4	0.0	9.852274988817668E-5	9.852274988817668E-5
28-29	0.0	3.2840916629392226E-4	0.0	3.2840916629392226E-4	1.0946972209797409E-4
30-31	0.0	4.159849439723015E-4	0.0	9.08598693413185E-4	1.204166943077715E-4
32-33	0.0	4.269319161820989E-4	0.0	0.0022331823307986717	1.5325761093716372E-4
34-35	0.0	5.145076938604782E-4	0.0	0.004061326689834839	1.7515155535675854E-4
36-37	0.0	6.020834715388574E-4	0.0	0.0068309106589135825	1.8609852756655594E-4
38-39	0.0	6.130304437486549E-4	0.0	0.01111117679294437	1.9704549977635335E-4
40-41	0.0	7.006062214270341E-4	0.0	0.015774586954318066	1.9704549977635335E-4
42-43	0.0	7.006062214270341E-4	0.0	0.020251898588125206	1.9704549977635335E-4
44-45	0.0	7.115531936368316E-4	0.0	0.024882467832869508	1.9704549977635335E-4
46-47	0.0	7.22500165846629E-4	0.0	0.030323113021138822	1.9704549977635335E-4
48-49	0.0	7.22500165846629E-4	0.0	0.035424402070904415	2.1893944419594817E-4
50-51	0.0	8.210229157348057E-4	0.0	0.041215350369887244	2.1893944419594817E-4
52-53	0.0	8.31969887944603E-4	0.0	0.04649179097500959	2.5178036082534037E-4
54-55	0.0	8.429168601544005E-4	0.0	0.05174633763571235	2.846212774547326E-4
56-57	0.0	8.538638323641979E-4	0.0	0.05719792979619146	2.846212774547326E-4
58-59	0.0	8.648108045739952E-4	0.0	0.06318592359495065	2.846212774547326E-4
60-61	0.0	8.976517212033876E-4	0.0	0.06852804603333178	2.846212774547326E-4
62-63	0.0	8.976517212033876E-4	0.0	0.07381543361066392	2.846212774547326E-4
64-65	0.0	0.001018068415511159	0.0	0.07891672266042951	2.846212774547326E-4
66-67	0.0	0.0010509093321405512	0.0	0.08442304968195762	2.9556824966453E-4
68-69	0.0	0.0011275381376091333	0.0	0.0895681266205624	3.0651522187432744E-4
70-71	0.0	0.0011713260264483228	0.0	0.09397975642111076	3.0651522187432744E-4
72-73	0.0	0.0011822729986581202	0.0	0.09790971944442803	3.0651522187432744E-4
74-75	0.0	0.0011822729986581202	0.0	0.10073403827455575	3.0651522187432744E-4
76-77	0.0	0.0012151139152875123	0.0	0.10355835710468347	3.0651522187432744E-4
78-79	0.0	0.0012479548319169046	0.0	0.1056163878801254	3.0651522187432744E-4
80-81	0.0	0.0012479548319169046	0.0	0.10633888804597202	3.3935613850371964E-4
82-83	0.0	0.0012479548319169046	0.0	0.10698475940635008	3.721970551331119E-4
84-85	0.0	0.0012698487763364995	0.0	0.10721464582275582	3.721970551331119E-4
86-87	0.0	0.0013245836373854865	0.0	0.107313168572644	3.721970551331119E-4
88	0.0	0.0014231063872736632	0.0	0.1073241155448538	3.940909995527067E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5230	0.0	50.329807	1
GTATCAA	9575	0.0	37.30901	1
CTTATAC	3825	0.0	36.97795	1
TCAACGC	11415	0.0	30.696167	4
ATCAACG	11495	0.0	30.556885	3
CAACGCA	11750	0.0	29.711899	5
TATCAAC	12070	0.0	29.349012	2
AACGCAG	12140	0.0	28.686687	6
TATACAC	5665	0.0	28.663546	3
ACGCAGA	13825	0.0	25.190336	7
CGCAGAG	14125	0.0	24.655321	8
TTATACA	6310	0.0	24.176037	2
ACATCTC	6860	0.0	21.988323	8
ACACATC	6880	0.0	21.61386	6
TACACAT	7670	0.0	21.05924	5
GCAGAGT	16870	0.0	20.567545	9
TATGCCG	4200	0.0	20.21636	42-43
GTGGTAT	2625	0.0	20.185457	1
CTCGTAT	4140	0.0	19.900913	38-39
GTACATG	12690	0.0	19.833525	1
>>END_MODULE