FastQCFastQC Report
Fri 27 May 2016
SRR2140056_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140056_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5035397
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56000.048.46141
GTATCAA102350.036.927471
ATCAACG115000.032.1150973
TCAACGC115600.031.9110874
CAACGCA118250.031.1959535
AACGCAG123300.030.090536
TATCAAC125100.029.7981782
ACGCAGA140500.026.345447
CGCAGAG142900.025.8123998
CTTATAC33000.023.0108171
GTGGTAT30250.021.2517241
GCAGAGT173700.021.1857419
TGGTATC26850.021.0503982
GTACATG122900.020.6774371
GAGTACT105300.020.25967212-13
TACATGG123850.019.5434022
ACATGGG121950.019.0341633
CAGAGTA169200.018.86810-11
AGAGTAC152500.018.82663510-11
TATACAC56200.018.4250323
Produced by FastQC (version 0.11.5)