##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140056_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5035397
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.605999685824177	32.0	18.0	33.0	18.0	33.0
2	31.102213589117202	32.0	31.0	33.0	27.0	33.0
3	32.36545738101683	33.0	33.0	33.0	30.0	34.0
4	32.695457577624964	33.0	33.0	34.0	31.0	34.0
5	33.1006311915426	33.0	33.0	34.0	33.0	34.0
6	36.98909420647468	38.0	37.0	38.0	35.0	38.0
7	37.31923163158734	38.0	38.0	38.0	36.0	38.0
8	37.264910790549386	38.0	38.0	38.0	36.0	38.0
9	37.4205922591605	38.0	38.0	38.0	37.0	38.0
10-11	37.45882459714696	38.0	38.0	38.0	37.0	38.0
12-13	37.47928058105448	38.0	38.0	38.0	37.0	38.0
14-15	37.43461190845528	38.0	38.0	38.0	37.0	38.0
16-17	37.45165316657257	38.0	38.0	38.0	37.0	38.0
18-19	37.37270040475458	38.0	38.0	38.0	37.0	38.0
20-21	37.46161280835277	38.0	38.0	38.0	37.0	38.0
22-23	37.47737909008805	38.0	38.0	38.0	37.0	38.0
24-25	37.483143288842655	38.0	38.0	38.0	37.0	38.0
26-27	37.46604005294063	38.0	38.0	38.0	37.0	38.0
28-29	37.402847837782225	38.0	38.0	38.0	37.0	38.0
30-31	37.444657296199296	38.0	38.0	38.0	37.0	38.0
32-33	37.4386068321503	38.0	38.0	38.0	37.0	38.0
34-35	37.41917761060071	38.0	38.0	38.0	37.0	38.0
36-37	37.402009023667205	38.0	38.0	38.0	37.0	38.0
38-39	37.385298014505665	38.0	38.0	38.0	37.0	38.0
40-41	37.37456150849191	38.0	38.0	38.0	37.0	38.0
42-43	37.31129197268751	38.0	38.0	38.0	37.0	38.0
44-45	37.321498971658386	38.0	38.0	38.0	37.0	38.0
46-47	37.30788758244317	38.0	38.0	38.0	37.0	38.0
48-49	37.27441610826884	38.0	38.0	38.0	37.0	38.0
50-51	37.240411833245105	38.0	38.0	38.0	37.0	38.0
52-53	37.21855498443921	38.0	38.0	38.0	37.0	38.0
54-55	37.20277789282508	38.0	38.0	38.0	37.0	38.0
56-57	37.202769222641194	38.0	38.0	38.0	37.0	38.0
58-59	37.192335753370266	38.0	38.0	38.0	37.0	38.0
60-61	37.19004766914647	38.0	38.0	38.0	37.0	38.0
62-63	37.189683009475445	38.0	38.0	38.0	36.5	38.0
64-65	37.18693802022973	38.0	38.0	38.0	36.5	38.0
66-67	37.1852755018444	38.0	38.0	38.0	36.0	38.0
68-69	37.18444116857043	38.0	38.0	38.0	36.0	38.0
70-71	37.18786314723695	38.0	38.0	38.0	36.0	38.0
72-73	37.15756909020781	38.0	38.0	38.0	36.0	38.0
74-75	37.15236511511136	38.0	38.0	38.0	36.0	38.0
76-77	37.14546883433194	38.0	38.0	38.0	36.0	38.0
78-79	37.12685686922763	38.0	38.0	38.0	36.0	38.0
80-81	37.11420228682734	38.0	38.0	38.0	36.0	38.0
82-83	37.111548748241304	38.0	38.0	38.0	36.0	38.0
84-85	37.09141698357996	38.0	38.0	38.0	36.0	38.0
86-87	37.06839709140804	38.0	38.0	38.0	36.0	38.0
88-89	37.04955330755777	38.0	38.0	38.0	36.0	38.0
90-91	37.0320404750851	38.0	38.0	38.0	36.0	38.0
92-93	37.02378199409356	38.0	38.0	38.0	36.0	38.0
94-95	36.98096695710498	38.0	38.0	38.0	36.0	38.0
96-97	36.99291916630669	38.0	38.0	38.0	36.0	38.0
98-99	36.98128640927443	38.0	38.0	38.0	36.0	38.0
100	35.27711707677732	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	11.0
20	72.0
21	185.0
22	426.0
23	945.0
24	2004.0
25	3830.0
26	6241.0
27	10501.0
28	15835.0
29	23475.0
30	32750.0
31	43900.0
32	58168.0
33	78309.0
34	115896.0
35	210322.0
36	658379.0
37	3774148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2690212906748	19.08056107591914	14.318394358975073	23.332023274430995
2	14.016301031082614	20.648959057168796	43.13269268082076	22.202047230927832
3	17.84667624022495	26.30789588189372	30.635121719300386	25.21030615858094
4	12.055414895786768	18.51264160502141	39.586253079945834	29.84569041924599
5	13.232589301939552	37.57470750051091	36.07371557119499	13.118987626354556
6	29.353532019548883	38.34241209744573	19.222552750270737	13.081503132734648
7	26.007542205708905	32.85077621486449	23.527400123565233	17.614281455861374
8	23.667448663928585	37.25519556849242	21.680892291114283	17.396463476464717
9	25.031611312033785	17.417450635810873	21.84359038571262	35.707347666442715
10-11	23.60473265563768	27.161582294305692	29.50453559073892	19.729149459317707
12-13	24.49310352291984	24.573335528459822	29.53949807731148	21.394062871308854
14-15	21.69311791037707	26.147642990973996	27.069179159972805	25.09005993867613
16-17	21.354582369572846	29.277135447314283	27.797808196652618	21.570473986460254
18-19	21.398213487437037	28.40580395150571	29.420748751290116	20.775233809767137
20-21	22.07243799815882	27.535211274598442	29.39227019666639	21.000080530576348
22-23	21.80656604514027	27.52898671323977	29.205868719611665	21.458578522008295
24-25	21.617431756876794	27.63853068757076	29.315444138022396	21.42859341753005
26-27	21.600548207772732	27.792793351987626	29.22236429498941	21.384294145250234
28-29	21.46956170855048	27.752014652960742	29.37255836140831	21.405865277080473
30-31	21.80841259839827	27.54058956546394	29.194255097940463	21.456742738197327
32-33	21.669218013263816	27.66548002162172	29.2667736599057	21.39852830520877
34-35	21.779287448232196	27.817698675889975	29.150012905147264	21.253000970730568
36-37	21.652991865222354	27.68825819136096	29.171512134466788	21.487237808949896
38-39	21.5305388821417	27.718201437024415	29.34433396225124	21.406925718582652
40-41	21.789245833256615	27.614441405591624	29.21482333388436	21.381489427267404
42-43	21.773701064763376	27.665437848389512	29.0924693551491	21.46839173169802
44-45	21.73887629117471	27.844945302398198	28.97433768673619	21.4418407196909
46-47	21.834240069307288	27.69287176604665	28.759626441968038	21.713261722678027
48-49	21.754225148838994	27.79107533355126	28.76388770841044	21.69081180919931
50-51	21.658696564116887	27.92868961719964	28.801433373266683	21.611180445416785
52-53	21.847331254823118	27.863876752307775	28.752152298801537	21.536639694067567
54-55	21.837193933497648	27.779998555463365	28.69004602896137	21.692761482077614
56-57	21.916938478637963	27.950138468636677	28.55035443654196	21.582568616183405
58-59	21.76291628383795	28.06176664570125	28.719625542375077	21.455691528085723
60-61	21.907240512371278	28.140877692105327	28.562458470859763	21.389423324663632
62-63	21.799847741417526	28.245705210418127	28.495016614201152	21.4594304339632
64-65	21.7841817999115	28.21674373588558	28.49380948771153	21.50526497649139
66-67	21.855221883860736	28.21295791878124	28.344905562916235	21.586914634441793
68-69	21.82780465158745	28.150732816206137	28.444242898661066	21.57721963354535
70-71	21.852090061281892	27.903552356839068	28.507482460153184	21.736875121725856
72-73	21.903099751440152	27.808160619376043	28.51797863613865	21.77076099304515
74-75	21.788962536997282	27.932544696430483	28.45581688461694	21.822675881955295
76-77	21.9300533496924	27.899564918180808	28.348228859810497	21.822152872316295
78-79	21.86329099175465	27.927929620950685	28.35869016377827	21.850089223516388
80-81	21.84372624191684	27.897070959174243	28.375074849261207	21.88412794964771
82-83	21.9075527727097	27.89590794114913	28.41699758227903	21.779541703862144
84-85	22.01633361488077	27.872001193799772	28.303389338576007	21.808275852743446
86-87	21.85452887505404	27.875028693995535	28.40444814196936	21.865994288981067
88-89	21.95403383353715	27.88121960553578	28.31750122060687	21.847245340320203
90-91	21.980938152620023	27.939281301656692	28.289788885343636	21.789991660379645
92-93	21.991760194992235	27.94142803122577	28.29399238721034	21.77281938657166
94-95	21.91384196104434	28.021759455280677	28.239752179895543	21.82464640377944
96-97	21.99424698075912	28.11908196282358	28.320032131429905	21.566638924987398
98-99	23.057359348703528	30.01017563917253	28.574403370671792	18.35806164145215
100	21.902660637377277	30.958111364464212	22.570860316610954	24.568367681547556
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	593.0
1	567.5
2	906.5
3	1415.5
4	1805.0
5	2357.5
6	2898.0
7	3753.5
8	5067.5
9	6120.5
10	6419.0
11	6544.0
12	7164.0
13	8419.0
14	10495.5
15	12695.5
16	14290.5
17	15204.5
18	15298.5
19	14723.0
20	14264.5
21	14629.0
22	16505.0
23	20145.0
24	25874.5
25	33969.0
26	44249.5
27	55796.0
28	67836.5
29	80664.0
30	93311.0
31	105891.5
32	118625.5
33	130891.5
34	142634.5
35	153726.0
36	165457.5
37	175531.5
38	182673.5
39	188625.5
40	195102.0
41	202140.0
42	206098.5
43	208386.5
44	213463.0
45	219076.0
46	216181.0
47	210651.0
48	206340.0
49	198615.5
50	191654.0
51	183323.5
52	172649.5
53	161886.5
54	150588.0
55	138502.0
56	125863.5
57	112730.5
58	99961.5
59	86344.0
60	71047.0
61	57303.0
62	45418.5
63	34736.5
64	26000.5
65	19144.0
66	14081.0
67	10729.5
68	8157.0
69	5892.0
70	4244.0
71	3127.0
72	2251.5
73	1535.5
74	1024.5
75	651.0
76	378.5
77	238.5
78	164.0
79	94.5
80	63.5
81	47.0
82	35.0
83	25.0
84	17.0
85	13.5
86	8.5
87	4.5
88	3.0
89	2.0
90	1.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.9859407311876304E-4
3	0.0
4	0.0
5	0.005163445901087839
6	3.971881462375261E-4
7	0.0
8	0.0
9	3.1775051699002083E-4
10-11	0.0
12-13	0.0
14-15	9.929703655938151E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	9.930487579988835E-5
26-27	9.931299244347302E-6
28-29	5.562976168408772E-4
30-31	2.2865348250686535E-4
32-33	0.0013941590313223718
34-35	2.495188527961532E-4
36-37	0.0013219289587365884
38-39	6.94394615201224E-4
40-41	7.284021184361611E-4
42-43	9.154084293045835E-5
44-45	1.4331720571168202E-4
46-47	4.641091502213027E-4
48-49	0.001506602608001847
50-51	0.0016427897122439316
52-53	0.0016748306761986652
54-55	0.00153784574749591
56-57	0.0029276406891623444
58-59	3.445177429867492E-4
60-61	1.0848427292646957E-5
62-63	0.0019348971602159346
64-65	0.002457016804782963
66-67	0.0030449384011184038
68-69	8.412209952608974E-4
70-71	0.004472517512170626
72-73	0.0010180680774116088
74-75	0.003876538255952361
76-77	3.1423834908947694E-4
78-79	9.513639270047822E-4
80-81	0.0
82-83	0.0
84-85	8.571911151054339E-4
86-87	0.0
88-89	0.0
90-91	0.0027334122875331056
92-93	7.028145085513072E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	23.0
20-21	94.0
22-23	228.0
24-25	318.0
26-27	973.0
28-29	2455.0
30-31	8032.0
32-33	10337.0
34-35	14842.0
36-37	23024.0
38-39	26446.0
40-41	25819.0
42-43	29858.0
44-45	35681.0
46-47	36422.0
48-49	34325.0
50-51	32074.0
52-53	31617.0
54-55	34442.0
56-57	35385.0
58-59	35193.0
60-61	35299.0
62-63	35342.0
64-65	37748.0
66-67	40724.0
68-69	42090.0
70-71	45802.0
72-73	37915.0
74-75	37189.0
76-77	38809.0
78-79	39501.0
80-81	39034.0
82-83	37663.0
84-85	37470.0
86-87	38791.0
88-89	39940.0
90-91	40649.0
92-93	39855.0
94-95	47708.0
96-97	154006.0
98-99	339821.0
100-101	3412453.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.53474919480563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.66505661737155	81.1554279754665
2	6.182706932815074	10.94768815283537
3	1.2966953184180456	3.4440778439466095
4	0.41003215913002894	1.45208377481527
5	0.18228632841077208	0.806933718374484
6	0.09432272762575462	0.5010503420229689
7	0.05237638323049254	0.32459909671298764
8	0.03241068061053335	0.22955771832692157
9	0.021414123491978354	0.17063046473300092
>10	0.0613611602658045	0.8038826110668633
>50	8.656105464932571E-4	0.055873988409591055
>100	4.4086608554222846E-4	0.0865579165229128
>500	3.109199788742028E-5	0.021636396766535507
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.9859407311876302E-5	0.0
3	0.0	0.0	0.0	1.9859407311876302E-5	0.0
4	0.0	1.9859407311876302E-5	0.0	1.9859407311876302E-5	0.0
5	0.0	1.9859407311876302E-5	0.0	3.9718814623752604E-5	0.0
6	0.0	1.9859407311876302E-5	0.0	3.9718814623752604E-5	3.9718814623752604E-5
7	0.0	1.9859407311876302E-5	0.0	5.957822193562891E-5	3.9718814623752604E-5
8	0.0	1.9859407311876302E-5	0.0	5.957822193562891E-5	3.9718814623752604E-5
9	0.0	9.929703655938152E-5	0.0	5.957822193562891E-5	5.957822193562891E-5
10-11	1.9859407311876302E-5	9.929703655938152E-5	0.0	1.1915644387125782E-4	5.957822193562891E-5
12-13	1.9859407311876302E-5	1.0922674021531968E-4	0.0	1.7873466580688673E-4	5.957822193562891E-5
14-15	2.9789110967814455E-5	1.6880496215094859E-4	0.0	1.7873466580688673E-4	5.957822193562891E-5
16-17	4.9648518279690754E-5	1.7873466580688673E-4	0.0	1.7873466580688673E-4	7.943762924750521E-5
18-19	5.957822193562891E-5	1.9859407311876301E-4	0.0	1.9859407311876301E-4	7.943762924750521E-5
20-21	5.957822193562891E-5	2.1845348043063933E-4	0.0	2.3831288774251564E-4	7.943762924750521E-5
22-23	5.957822193562891E-5	2.1845348043063933E-4	0.0	2.581722950543919E-4	9.929703655938151E-5
24-25	6.950792559156705E-5	2.780317023662682E-4	0.0	2.9789110967814455E-4	1.1915644387125782E-4
26-27	7.943762924750521E-5	2.9789110967814455E-4	0.0	3.8725844258158794E-4	1.1915644387125782E-4
28-29	7.943762924750521E-5	2.9789110967814455E-4	0.0	5.461337010765983E-4	1.1915644387125782E-4
30-31	7.943762924750521E-5	3.1775051699002083E-4	0.0	0.0011220565131210111	1.1915644387125782E-4
32-33	1.0922674021531968E-4	3.3760992430189717E-4	0.0	0.002611512061511734	1.1915644387125782E-4
34-35	1.2908614752719596E-4	3.475396279578353E-4	0.0	0.00461731220001124	1.3901585118313413E-4
36-37	1.3901585118313413E-4	3.8725844258158794E-4	0.0	0.007437348038297676	1.3901585118313413E-4
38-39	1.3901585118313413E-4	4.56766368173155E-4	0.0	0.011568104759167946	1.3901585118313413E-4
40-41	1.3901585118313413E-4	5.163445901087838E-4	0.0	0.015510197110575392	1.3901585118313413E-4
42-43	1.3901585118313413E-4	5.262742937647219E-4	0.0	0.019819688497252554	1.4894555483907227E-4
44-45	1.3901585118313413E-4	5.858525157003509E-4	0.0	0.025231376989738843	1.5887525849501042E-4
46-47	1.3901585118313413E-4	6.355010339800417E-4	0.0	0.031179269479645794	1.5887525849501042E-4
48-49	1.3901585118313413E-4	6.355010339800417E-4	0.0	0.036849130267186475	1.9859407311876304E-4
50-51	1.3901585118313413E-4	7.24868366883485E-4	0.0	0.0423700454998881	1.9859407311876304E-4
52-53	1.3901585118313413E-4	7.347980705394232E-4	0.0	0.04750370229000812	2.0852377677470118E-4
54-55	1.3901585118313413E-4	7.347980705394232E-4	0.0	0.05274658582034346	2.1845348043063933E-4
56-57	1.3901585118313413E-4	7.347980705394232E-4	0.0	0.058664689199282596	2.1845348043063933E-4
58-59	1.3901585118313413E-4	7.546574778512995E-4	0.0	0.06438419850510298	2.283831840865775E-4
60-61	1.3901585118313413E-4	7.84446588819114E-4	0.0	0.07009377810726741	2.3831288774251564E-4
62-63	1.3901585118313413E-4	7.943762924750522E-4	0.0	0.07596223296792687	2.581722950543919E-4
64-65	1.3901585118313413E-4	8.638842180666191E-4	0.0	0.08131434323847753	2.581722950543919E-4
66-67	1.3901585118313413E-4	8.738139217225573E-4	0.0	0.08672603173096381	2.681019987103301E-4
68-69	1.3901585118313413E-4	8.837436253784955E-4	0.0	0.09208807170517042	2.7803170236626826E-4
70-71	1.3901585118313413E-4	8.936733290344336E-4	0.0	0.09685432946002073	2.7803170236626826E-4
72-73	1.3901585118313413E-4	8.936733290344336E-4	0.0	0.10031979603594314	2.7803170236626826E-4
74-75	1.3901585118313413E-4	9.333921436581863E-4	0.0	0.10316962098519739	2.7803170236626826E-4
76-77	1.3901585118313413E-4	9.631812546260006E-4	0.0	0.10601944593445164	2.7803170236626826E-4
78-79	1.3901585118313413E-4	9.731109582819388E-4	0.0	0.10758833911208987	2.7803170236626826E-4
80-81	1.3901585118313413E-4	9.731109582819388E-4	0.0	0.10815433222047835	2.7803170236626826E-4
82-83	1.4894555483907227E-4	9.731109582819388E-4	0.0	0.10866074710693119	2.7803170236626826E-4
84-85	1.5887525849501042E-4	9.731109582819388E-4	0.0	0.10891891940198559	2.7803170236626826E-4
86-87	1.5887525849501042E-4	9.731109582819388E-4	0.0	0.1089487085129534	2.7803170236626826E-4
88	1.5887525849501042E-4	0.0010724079948413203	0.0	0.1089487085129534	2.9789110967814455E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5600	0.0	48.4614	1
GTATCAA	10235	0.0	36.92747	1
ATCAACG	11500	0.0	32.115097	3
TCAACGC	11560	0.0	31.911087	4
CAACGCA	11825	0.0	31.195953	5
AACGCAG	12330	0.0	30.09053	6
TATCAAC	12510	0.0	29.798178	2
ACGCAGA	14050	0.0	26.34544	7
CGCAGAG	14290	0.0	25.812399	8
CTTATAC	3300	0.0	23.010817	1
GTGGTAT	3025	0.0	21.251724	1
GCAGAGT	17370	0.0	21.185741	9
TGGTATC	2685	0.0	21.050398	2
GTACATG	12290	0.0	20.677437	1
GAGTACT	10530	0.0	20.259672	12-13
TACATGG	12385	0.0	19.543402	2
ACATGGG	12195	0.0	19.034163	3
CAGAGTA	16920	0.0	18.868	10-11
AGAGTAC	15250	0.0	18.826635	10-11
TATACAC	5620	0.0	18.425032	3
>>END_MODULE