FastQCFastQC Report
Fri 27 May 2016
SRR2140055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5638159
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113250.055.985461
CTTATAC71000.044.854791
GTATCAA226500.040.423771
TATACAC90800.037.997163
TTATACA96850.034.338452
ATCAACG281350.032.3077933
TATCAAC284200.032.187512
TCAACGC285950.031.8315724
TGGTATC42300.031.4681052
GTGGTAT44400.031.3806741
CAACGCA290050.031.3673215
AACGCAG296750.030.7966636
ACGCAGA332750.027.3900287
TACACAT127250.027.0153395
CGCAGAG337700.026.963998
ACACATC121800.026.9284936
CCGAACC12500.026.205959
ACATCTC127000.025.9891578
ACCGAAC12350.025.8527418
GGACCGA13900.025.6546736
Produced by FastQC (version 0.11.5)