##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140055_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5638159
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.81765821077412	32.0	18.0	33.0	18.0	33.0
2	31.206995758721952	32.0	32.0	33.0	27.0	33.0
3	32.42765023121909	33.0	33.0	33.0	31.0	34.0
4	32.76205140720579	33.0	33.0	34.0	31.0	34.0
5	33.117111809014254	33.0	33.0	34.0	33.0	34.0
6	37.035076165819376	38.0	37.0	38.0	36.0	38.0
7	37.39567064355581	38.0	38.0	38.0	37.0	38.0
8	37.36385121455425	38.0	38.0	38.0	37.0	38.0
9	37.51124790911359	38.0	38.0	38.0	37.0	38.0
10-11	37.54155248193604	38.0	38.0	38.0	37.5	38.0
12-13	37.55455823434564	38.0	38.0	38.0	37.5	38.0
14-15	37.51866486915321	38.0	38.0	38.0	37.0	38.0
16-17	37.53540215875431	38.0	38.0	38.0	37.0	38.0
18-19	37.45486931106413	38.0	38.0	38.0	37.0	38.0
20-21	37.54933617900198	38.0	38.0	38.0	37.0	38.0
22-23	37.56256684594841	38.0	38.0	38.0	38.0	38.0
24-25	37.567182123876826	38.0	38.0	38.0	38.0	38.0
26-27	37.5473679647548	38.0	38.0	38.0	38.0	38.0
28-29	37.47661038183723	38.0	38.0	38.0	37.0	38.0
30-31	37.518489127276396	38.0	38.0	38.0	37.5	38.0
32-33	37.502501569347196	38.0	38.0	38.0	38.0	38.0
34-35	37.471280405483085	38.0	38.0	38.0	37.0	38.0
36-37	37.44199834746685	38.0	38.0	38.0	37.0	38.0
38-39	37.40293831757014	38.0	38.0	38.0	37.0	38.0
40-41	37.41515645418274	38.0	38.0	38.0	37.0	38.0
42-43	37.34078400481267	38.0	38.0	38.0	37.0	38.0
44-45	37.33120213865606	38.0	38.0	38.0	37.0	38.0
46-47	37.288532290304104	38.0	38.0	38.0	37.0	38.0
48-49	37.23493778276587	38.0	38.0	38.0	37.0	38.0
50-51	37.19635989981863	38.0	38.0	38.0	37.0	38.0
52-53	37.176320690678224	38.0	38.0	38.0	37.0	38.0
54-55	37.165270222342855	38.0	38.0	38.0	37.0	38.0
56-57	37.17262772583553	38.0	38.0	38.0	37.0	38.0
58-59	37.17150278200421	38.0	38.0	38.0	37.0	38.0
60-61	37.1703266632054	38.0	38.0	38.0	37.0	38.0
62-63	37.17751101998262	38.0	38.0	38.0	37.0	38.0
64-65	37.18451369475882	38.0	38.0	38.0	37.0	38.0
66-67	37.18850829881477	38.0	38.0	38.0	37.0	38.0
68-69	37.19543769635973	38.0	38.0	38.0	37.0	38.0
70-71	37.20602272869466	38.0	38.0	38.0	37.0	38.0
72-73	37.182104630633404	38.0	38.0	38.0	37.0	38.0
74-75	37.18800355864259	38.0	38.0	38.0	37.0	38.0
76-77	37.19470200169148	38.0	38.0	38.0	37.0	38.0
78-79	37.18554392368942	38.0	38.0	38.0	36.0	38.0
80-81	37.183799225159895	38.0	38.0	38.0	36.0	38.0
82-83	37.19093737442431	38.0	38.0	38.0	36.0	38.0
84-85	37.17662682974456	38.0	38.0	38.0	36.0	38.0
86-87	37.166431997059	38.0	38.0	38.0	36.0	38.0
88-89	37.151613371991466	38.0	38.0	38.0	36.0	38.0
90-91	37.14091321035349	38.0	38.0	38.0	36.0	38.0
92-93	37.142147346825396	38.0	38.0	38.0	36.0	38.0
94-95	37.10149352475176	38.0	38.0	38.0	36.0	38.0
96-97	37.113453773121876	38.0	38.0	38.0	36.0	38.0
98-99	37.10795721055018	38.0	38.0	38.0	36.0	38.0
100	35.53719240184647	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	16.0
20	49.0
21	168.0
22	391.0
23	906.0
24	1954.0
25	3464.0
26	6214.0
27	10425.0
28	17207.0
29	27004.0
30	37809.0
31	49307.0
32	62157.0
33	80234.0
34	114552.0
35	211024.0
36	736505.0
37	4278769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80463552021147	19.3953380881951	14.318379456840432	22.481646934752995
2	14.258643578694766	20.7023231931779	43.37534774291899	21.663685485208344
3	17.62002455056695	27.82796654014191	31.06187675799849	23.49013215129265
4	11.951383421432421	18.98671179723736	38.791101847251916	30.270802934078304
5	12.622681244005951	38.43800944432511	35.43124254263524	13.508066769033697
6	28.23127110069003	38.326151597732945	19.24957588001018	14.193001421566844
7	25.46716046851463	33.922402684989905	22.905189442156562	17.7052474043389
8	24.418812743242164	36.29712688029085	21.448249844763104	17.83581053170389
9	25.971467566016837	16.67890769038398	21.274674560515912	36.07495018308327
10-11	23.965074415247955	27.060384426902466	28.660880262511217	20.313660895338355
12-13	25.278623749347968	24.848935618878432	28.813669497437015	21.058771134336578
14-15	22.465072375575076	25.791255620850706	27.005189105167133	24.738482898407085
16-17	21.202027115588617	29.340800782666825	27.534244777417594	21.922927324326967
18-19	21.29166630455083	27.655818149151166	30.059608109668424	20.992907436629583
20-21	22.520018960313262	26.399859526958807	29.9687438615099	21.11137765121803
22-23	22.193857619390073	26.85417812373429	29.77436350141644	21.177600755459196
24-25	21.840274747457546	27.11453971889508	30.01670253383256	21.02848299981481
26-27	21.667584910336632	27.22597655901764	30.063460380350694	21.042978150295035
28-29	21.056129978674708	26.95263974810775	30.310987184413634	21.68024308880391
30-31	21.825344462331667	26.625686887581423	30.519458997250982	21.029509652835923
32-33	21.27717538493117	27.27932382966956	30.086146255313206	21.357354530086067
34-35	21.365994883928355	27.381942360570864	30.550333021992564	20.701729733508216
36-37	21.730265113530187	27.082455779601446	30.011273112508253	21.17600599436012
38-39	21.314313224577187	26.815267487812672	30.370502089967705	21.49991719764244
40-41	21.488147475777236	26.74511144902666	30.374536718177854	21.392204357018247
42-43	21.82663119563532	26.708284980048923	30.135624639822062	21.329459184493693
44-45	21.4806258233577	27.1489856530298	29.90879987251595	21.461588651096548
46-47	21.96114744222148	26.865204041432335	29.317119645987283	21.8565288703589
48-49	21.824257209236553	26.988171247806047	29.224561477819535	21.963010065137865
50-51	21.50600719969329	27.189794719853754	29.375840999317887	21.928357081135065
52-53	21.65757560099963	27.26839836869764	29.56935715484334	21.504668875459394
54-55	21.623113089051245	27.338704839571648	29.0896587723799	21.9485232989972
56-57	22.065720087552965	27.615496558583175	28.550838258352428	21.767945095511426
58-59	21.467191380781397	28.08902955862871	29.187722169271257	21.25605689131864
60-61	21.88054421801683	28.588502685461663	28.350848618813096	21.180104477708404
62-63	21.49946820666579	28.935993454411197	28.262208081930602	21.30233025699241
64-65	21.46887447535953	28.880944396764864	28.295403521698404	21.35477760617721
66-67	21.665105178035873	28.74339071585966	28.191042463767356	21.400461642337103
68-69	21.63564939096556	28.464875394736673	28.39805010388199	21.501425110415777
70-71	21.732019496703124	28.050665271958852	28.483267121040146	21.73404811029788
72-73	21.932894415813784	27.638191783551115	28.66354090307908	21.765372897556016
74-75	21.838425208841368	27.655281553186228	28.615202667576817	21.891090570395587
76-77	21.869150855941527	27.60621947038995	28.526547700063748	21.998081973604773
78-79	21.899036092240188	27.642749519050003	28.491530443131087	21.966683945578726
80-81	21.8890833346239	27.618481749928403	28.491222319894543	22.001212595553156
82-83	21.921144107764214	27.59957399246231	28.54752858834943	21.93175331142404
84-85	22.13195350549883	27.55349041892045	28.380360452596555	21.93419562298416
86-87	21.871406726727592	27.71045929083939	28.385436545944685	22.032697436488334
88-89	22.0875175493598	27.589934123712606	28.30796997176418	22.014578355163415
90-91	22.123065741050993	27.62667765581624	28.315750570787156	21.934506032345613
92-93	22.130396451999797	27.61802539189409	28.276455427285658	21.975122728820455
94-95	21.988246475747356	27.68003718171232	28.280478777866495	22.05123756467383
96-97	22.064858902842364	27.82613280448326	28.29480658504967	21.814201707624704
98-99	23.13992758431289	29.7370256620618	28.463968695110335	18.659078058514975
100	29.453701446464812	40.57426683211429	29.972031721420894	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1770.0
1	1642.0
2	2398.0
3	3553.0
4	4419.5
5	5885.0
6	7240.0
7	9123.0
8	11928.5
9	13730.0
10	13852.0
11	13890.5
12	15103.0
13	17862.0
14	23023.5
15	28664.0
16	32706.0
17	35025.5
18	34207.0
19	30639.5
20	26708.0
21	23819.5
22	23340.5
23	26395.5
24	32549.0
25	41448.0
26	53192.0
27	65913.0
28	79178.5
29	94168.0
30	107795.0
31	119698.5
32	133650.0
33	148975.5
34	161954.5
35	174101.0
36	186557.5
37	197221.0
38	205175.5
39	209385.0
40	213452.5
41	216650.0
42	222294.0
43	247333.0
44	258866.5
45	251203.5
46	245342.0
47	234058.5
48	225926.0
49	219716.0
50	213008.0
51	205053.0
52	196118.5
53	186971.0
54	176930.5
55	165440.5
56	151448.0
57	135965.0
58	121546.0
59	106254.0
60	90255.5
61	74665.0
62	58968.0
63	45516.0
64	35991.5
65	27447.5
66	19529.0
67	14723.0
68	11198.5
69	8057.0
70	6072.5
71	4549.0
72	3341.0
73	2467.0
74	1793.5
75	1291.0
76	843.0
77	499.5
78	380.5
79	278.5
80	174.0
81	117.5
82	95.0
83	72.0
84	51.5
85	37.0
86	22.0
87	12.5
88	12.0
89	11.0
90	9.0
91	5.0
92	2.0
93	2.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.4830800266540905E-4
3	0.0
4	0.0
5	0.004895214909689492
6	2.4830800266540905E-4
7	0.0
8	3.5472571809344146E-5
9	2.4830800266540905E-4
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.321535135169653E-5
26-27	0.0
28-29	5.590869240838939E-4
30-31	2.3103165675733385E-4
32-33	0.0015322890723913937
34-35	3.0392058447861487E-4
36-37	0.0012666258112468826
38-39	7.239497977012784E-4
40-41	7.93741449032493E-4
42-43	1.5634893956331742E-4
44-45	3.714568817494876E-5
46-47	4.977339955511972E-4
48-49	0.0017569354790612228
50-51	0.0019581737917635754
52-53	0.001967885275584399
54-55	0.0013015456098768717
56-57	0.003153626388596566
58-59	5.295068702517345E-4
60-61	3.0296264137372957E-5
62-63	0.002257682710379763
64-65	0.0024180800465098064
66-67	0.003475281848498228
68-69	0.0010092748105511502
70-71	0.005233117585023055
72-73	0.001165468231809872
74-75	0.004196577485128031
76-77	2.478355228287496E-4
78-79	9.126102661354058E-4
80-81	0.0
82-83	0.0
84-85	0.0011025286435163313
86-87	0.0
88-89	1.2158557314223324E-5
90-91	0.003302551011165949
92-93	5.620849705098998E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	39.0
20-21	152.0
22-23	397.0
24-25	633.0
26-27	1729.0
28-29	5068.0
30-31	13609.0
32-33	17528.0
34-35	24186.0
36-37	37797.0
38-39	46087.0
40-41	42924.0
42-43	49459.0
44-45	59077.0
46-47	60171.0
48-49	57242.0
50-51	52398.0
52-53	47848.0
54-55	50921.0
56-57	52951.0
58-59	52908.0
60-61	57281.0
62-63	55297.0
64-65	58553.0
66-67	66995.0
68-69	73673.0
70-71	89830.0
72-73	58679.0
74-75	52704.0
76-77	54896.0
78-79	56369.0
80-81	57118.0
82-83	53387.0
84-85	51454.0
86-87	53080.0
88-89	54268.0
90-91	55126.0
92-93	54010.0
94-95	59566.0
96-97	160994.0
98-99	1190293.0
100-101	2551462.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.94703637996864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1669259622806	74.8529917764554
2	7.369521560128655	12.372989890221668
3	1.9478379924525773	4.905456804441047
4	0.7230713887734777	2.427988007147263
5	0.30966442647998804	1.2997705437648839
6	0.16301351827042726	0.8210701049204544
7	0.0975957308147604	0.5735010655665422
8	0.06059799463560404	0.40696176481825574
9	0.042169553423918316	0.31860081318642314
>10	0.1154379899779658	1.4603102840703932
>50	0.0025197186756139777	0.14641145287870505
>100	0.0013966587065222743	0.2407238158406374
>500	1.767895585139724E-4	0.09697082893624194
>1k	7.071582140261822E-5	0.07625284775207603
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.7736285904672076E-5	0.0
5	0.0	0.0	0.0	1.7736285904672076E-5	0.0
6	0.0	0.0	0.0	1.7736285904672076E-5	0.0
7	0.0	0.0	0.0	3.547257180934415E-5	0.0
8	0.0	0.0	0.0	3.547257180934415E-5	0.0
9	0.0	0.0	0.0	5.320885771401622E-5	0.0
10-11	0.0	0.0	0.0	7.09451436186883E-5	0.0
12-13	0.0	0.0	0.0	7.09451436186883E-5	0.0
14-15	0.0	0.0	0.0	7.09451436186883E-5	0.0
16-17	0.0	0.0	0.0	8.868142952336037E-5	0.0
18-19	0.0	0.0	0.0	8.868142952336037E-5	0.0
20-21	0.0	0.0	0.0	9.75495724756964E-5	0.0
22-23	0.0	0.0	0.0	1.0641771542803244E-4	0.0
24-25	0.0	0.0	0.0	1.5962657314204867E-4	0.0
26-27	0.0	0.0	0.0	2.128354308560649E-4	0.0
28-29	0.0	0.0	0.0	5.764292919018424E-4	0.0
30-31	0.0	0.0	0.0	0.0016051338743728228	0.0
32-33	0.0	0.0	0.0	0.004043873186265233	0.0
34-35	0.0	0.0	0.0	0.0075113170806286236	0.0
36-37	0.0	0.0	0.0	0.012495213419841476	0.0
38-39	0.0	0.0	0.0	0.019820299498471042	0.0
40-41	0.0	0.0	0.0	0.027287275864337986	0.0
42-43	0.0	0.0	0.0	0.0344527353698255	0.0
44-45	0.0	0.0	0.0	0.04223009673902421	0.0
46-47	0.0	0.0	0.0	0.05132881140812098	0.0
48-49	0.0	0.0	0.0	0.06015261364569534	0.0
50-51	0.0	0.0	0.0	0.0690296247409837	0.0
52-53	0.0	0.0	0.0	0.07757851454703565	0.0
54-55	0.0	0.0	0.0	0.08499228205518858	0.0
56-57	0.0	0.0	0.0	0.09308002842771905	0.0
58-59	0.0	0.0	0.0	0.1014338190888196	0.0
60-61	0.0	0.0	0.0	0.10983195046468182	0.0
62-63	0.0	0.0	0.0	0.11783988355064126	0.0
64-65	0.0	0.0	0.0	0.12546648648965025	0.0
66-67	0.0	0.0	0.0	0.13272062742466112	0.0
68-69	0.0	0.0	0.0	0.1394781523543412	0.0
70-71	0.0	0.0	0.0	0.1448433788405045	0.0
72-73	0.0	0.0	0.0	0.1492685821737202	0.0
74-75	0.0	0.0	0.0	0.15307301550027236	0.0
76-77	0.0	0.0	0.0	0.15653159125168342	0.0
78-79	0.0	0.0	0.0	0.1587486269897674	0.0
80-81	0.0	0.0	0.0	0.15940486956824026	0.0
82-83	0.0	0.0	0.0	0.15993695814538045	0.0
84-85	0.0	0.0	0.0	0.16021187057690284	0.0
86-87	0.0	0.0	0.0	0.16032715643528322	0.0
88	0.0	0.0	0.0	0.16033602457823556	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11325	0.0	55.98546	1
CTTATAC	7100	0.0	44.85479	1
GTATCAA	22650	0.0	40.42377	1
TATACAC	9080	0.0	37.99716	3
TTATACA	9685	0.0	34.33845	2
ATCAACG	28135	0.0	32.307793	3
TATCAAC	28420	0.0	32.18751	2
TCAACGC	28595	0.0	31.831572	4
TGGTATC	4230	0.0	31.468105	2
GTGGTAT	4440	0.0	31.380674	1
CAACGCA	29005	0.0	31.367321	5
AACGCAG	29675	0.0	30.796663	6
ACGCAGA	33275	0.0	27.390028	7
TACACAT	12725	0.0	27.015339	5
CGCAGAG	33770	0.0	26.96399	8
ACACATC	12180	0.0	26.928493	6
CCGAACC	1250	0.0	26.20595	9
ACATCTC	12700	0.0	25.989157	8
ACCGAAC	1235	0.0	25.852741	8
GGACCGA	1390	0.0	25.654673	6
>>END_MODULE