FastQCFastQC Report
Fri 27 May 2016
SRR2140054_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140054_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6049998
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112450.055.209421
GTATCAA221800.038.9602931
TGGTATC40600.032.9555552
GTGGTAT41750.032.8366661
ATCAACG275300.031.0151463
TCAACGC277350.030.8007454
TATCAAC280650.030.6292762
CAACGCA284150.030.0491645
AACGCAG291850.029.297236
ACGCAGA328050.026.0392117
CGCAGAG331350.025.7674568
GCAGAGT365700.023.1219929
CTTATAC45450.023.007591
GAGTACT224100.021.04277412-13
GTACATG250850.020.1209131
TATACAC62250.020.038933
TACATGG246550.020.0209872
CAGAGTA366300.019.80806210-11
ACATGGG248750.019.3474043
GTACTTT246600.018.9310714-15
Produced by FastQC (version 0.11.5)