##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140054_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6049998
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.405013191739897	32.0	18.0	33.0	18.0	33.0
2	31.020908436663944	32.0	31.0	33.0	27.0	33.0
3	32.348815652501045	33.0	33.0	33.0	30.0	34.0
4	32.6878096488627	33.0	33.0	34.0	31.0	34.0
5	33.07617638881864	33.0	33.0	34.0	33.0	34.0
6	37.009517027939516	38.0	37.0	38.0	35.0	38.0
7	37.385328061265476	38.0	38.0	38.0	37.0	38.0
8	37.35411284433482	38.0	38.0	38.0	37.0	38.0
9	37.50533355548217	38.0	38.0	38.0	37.0	38.0
10-11	37.53789819434651	38.0	38.0	38.0	37.5	38.0
12-13	37.554880183431465	38.0	38.0	38.0	37.5	38.0
14-15	37.51889603930448	38.0	38.0	38.0	37.0	38.0
16-17	37.535522821660436	38.0	38.0	38.0	37.0	38.0
18-19	37.46343064906799	38.0	38.0	38.0	37.0	38.0
20-21	37.55028718931205	38.0	38.0	38.0	37.5	38.0
22-23	37.56412620774171	38.0	38.0	38.0	38.0	38.0
24-25	37.56617786059405	38.0	38.0	38.0	38.0	38.0
26-27	37.54672058357558	38.0	38.0	38.0	38.0	38.0
28-29	37.48018831279427	38.0	38.0	38.0	37.5	38.0
30-31	37.51800789372508	38.0	38.0	38.0	38.0	38.0
32-33	37.50391605542546	38.0	38.0	38.0	38.0	38.0
34-35	37.47505210312339	38.0	38.0	38.0	37.5	38.0
36-37	37.446889444021565	38.0	38.0	38.0	37.0	38.0
38-39	37.42003850537777	38.0	38.0	38.0	37.0	38.0
40-41	37.40155495125545	38.0	38.0	38.0	37.0	38.0
42-43	37.32053968809697	38.0	38.0	38.0	37.0	38.0
44-45	37.3095909881076	38.0	38.0	38.0	37.0	38.0
46-47	37.2629795507215	38.0	38.0	38.0	37.0	38.0
48-49	37.20257158588586	38.0	38.0	38.0	37.0	38.0
50-51	37.16158361528362	38.0	38.0	38.0	37.0	38.0
52-53	37.14516924338662	38.0	38.0	38.0	37.0	38.0
54-55	37.13500278786888	38.0	38.0	38.0	37.0	38.0
56-57	37.14403865250525	38.0	38.0	38.0	37.0	38.0
58-59	37.144962889917736	38.0	38.0	38.0	37.0	38.0
60-61	37.15234122192559	38.0	38.0	38.0	37.0	38.0
62-63	37.16463255483292	38.0	38.0	38.0	37.0	38.0
64-65	37.171441267165825	38.0	38.0	38.0	37.0	38.0
66-67	37.181730695707365	38.0	38.0	38.0	37.0	38.0
68-69	37.19232224595924	38.0	38.0	38.0	37.0	38.0
70-71	37.207787395075044	38.0	38.0	38.0	37.0	38.0
72-73	37.187305852888805	38.0	38.0	38.0	37.0	38.0
74-75	37.19160052683061	38.0	38.0	38.0	37.0	38.0
76-77	37.194439523910575	38.0	38.0	38.0	37.0	38.0
78-79	37.182617274533484	38.0	38.0	38.0	36.0	38.0
80-81	37.179615313454974	38.0	38.0	38.0	36.0	38.0
82-83	37.182566330427335	38.0	38.0	38.0	36.0	38.0
84-85	37.17038354829651	38.0	38.0	38.0	36.0	38.0
86-87	37.160551580297906	38.0	38.0	38.0	36.0	38.0
88-89	37.14548455903167	38.0	38.0	38.0	36.0	38.0
90-91	37.13722283509689	38.0	38.0	38.0	36.0	38.0
92-93	37.13569629761324	38.0	38.0	38.0	36.0	38.0
94-95	37.099569687674574	38.0	38.0	38.0	36.0	38.0
96-97	37.1153654112303	38.0	38.0	38.0	36.0	38.0
98-99	37.11060706654047	38.0	38.0	38.0	36.0	38.0
100	35.55602330600075	38.0	36.0	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	16.0
20	59.0
21	147.0
22	387.0
23	916.0
24	1983.0
25	3925.0
26	6972.0
27	11799.0
28	19305.0
29	30035.0
30	42233.0
31	54993.0
32	69104.0
33	88562.0
34	125631.0
35	228287.0
36	794521.0
37	4571121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.88776657446829	19.28270058932251	14.455178332290359	22.374354503918845
2	14.326786426802594	21.110971900412263	42.91758223412896	21.64465943865618
3	17.6201215273129	27.359149540214723	31.11655574101016	23.90417319146221
4	12.134516408104599	18.912766582732754	39.219533626292105	29.733183382870536
5	12.838662092987274	37.996653711728605	35.580330754247164	13.584353441036958
6	28.430098682987165	38.365207548654304	19.386579688954292	13.818114079404243
7	25.387033185796092	33.87715169492618	23.466090402013357	17.26972471726437
8	23.987449911132188	36.35864612825428	21.786357910590702	17.867546050022835
9	25.776501668515984	16.918730569173125	21.878559408474764	35.42620835383613
10-11	23.723272305213985	27.25513297690346	29.002464133045997	20.019130584836557
12-13	24.93729749993306	25.106719043543485	29.20492700989323	20.75105644663023
14-15	22.127571127095507	26.14787857350354	27.56319320793108	24.161357091469874
16-17	20.901486248425204	29.3160344846395	28.167207658581045	21.61527160835425
18-19	20.928684604523838	27.86433152539885	30.763101078711102	20.44388279136621
20-21	21.997011863289522	26.83006431155168	30.69708809565211	20.475835729506684
22-23	21.744373591615794	26.896366477222244	30.460621443268128	20.898638487893837
24-25	21.365591267201232	27.098996973256394	30.641392088808505	20.894019670733872
26-27	21.214008784403173	27.255945790687637	30.666513346196933	20.86353207871226
28-29	21.07703709597235	27.22633851998706	30.719197770061722	20.977426613978864
30-31	21.428909801099895	27.16774811000013	30.524355084440035	20.87898700445994
32-33	21.35819293677676	27.121497437016846	30.48207739143443	21.038232234771964
34-35	21.364115027407255	27.231757323138737	30.52613153947275	20.877996109981257
36-37	21.25111106284404	27.287124803753116	30.47075855349618	20.991005579906666
38-39	21.12965704786689	27.204323662687024	30.58117286155243	21.084846427893662
40-41	21.391806031133314	27.077316834054123	30.44728130984519	21.08359582496737
42-43	21.450482629104105	27.14498286368081	30.178603574997254	21.22593093221783
44-45	21.382456306387727	27.32826876375361	30.03893329148716	21.250341638371502
46-47	21.522217786561406	27.368020396679622	29.617555210176917	21.49220660658206
48-49	21.51720857082694	27.45715527450975	29.44918963430421	21.5764465203591
50-51	21.480642057268348	27.657824492136236	29.341950397146732	21.51958305344868
52-53	21.636071583683847	27.593161065477556	29.293637461901696	21.4771298889369
54-55	21.59073884615232	27.612487614877285	29.13378218944907	21.662991349521324
56-57	21.697949462600967	27.7258618115168	28.962328672259385	21.613860053622854
58-59	21.527157379830378	27.884855566954048	29.188489444618654	21.399497608596914
60-61	21.77743337365102	27.910464177918254	28.998545278724823	21.313557169705895
62-63	21.576517737894196	28.02953974770909	28.94811427105324	21.44582824334347
64-65	21.566454259162963	28.00692702447755	28.92626134735788	21.500357369001605
66-67	21.672882933191815	27.993777758676025	28.789076298412088	21.544263009720073
68-69	21.65748621174625	27.85621358270889	28.933810457937003	21.552489747607858
70-71	21.645386309095144	27.652874689151158	28.99166985701437	21.71006914473933
72-73	21.764812471270623	27.579798541108424	28.943117617125154	21.7122713704958
74-75	21.673799876088676	27.671769875137763	28.8819998064671	21.772430442306458
76-77	21.781739665916515	27.572217954501394	28.774441810236652	21.87160056934544
78-79	21.78259056036998	27.636761626861766	28.706340062380598	21.874307750387658
80-81	21.760402295370472	27.692666063310895	28.6388981174318	21.908033523886832
82-83	21.81491443235363	27.688035931949535	28.71404658441999	21.783003051276854
84-85	22.018911155567455	27.62247405343562	28.54656507830262	21.81204971269431
86-87	21.792010295799596	27.71159482572404	28.58408880581913	21.912306072657238
88-89	21.884559153712402	27.673829999370366	28.497928078838868	21.943682768078368
90-91	21.98051421035342	27.723088838568433	28.471322470028817	21.82507448104933
92-93	21.96455698727979	27.719480380057703	28.399371257399697	21.916591375262804
94-95	21.907333935750913	27.80570200774785	28.368792872827846	21.91817118367339
96-97	21.93917757591375	27.877585412606148	28.45662247916242	21.726614532317683
98-99	23.003055016105435	29.807205835269716	28.639332181303857	18.55040696732099
100	29.01900303771597	40.792426485589104	30.188570476694938	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2097.0
1	1852.5
2	2647.0
3	4058.0
4	5013.5
5	6459.5
6	7936.5
7	10323.5
8	13558.0
9	15612.0
10	15752.5
11	15435.0
12	16713.5
13	20020.5
14	25849.0
15	32317.5
16	37099.5
17	39557.0
18	38542.0
19	34746.0
20	30698.5
21	27888.5
22	27977.5
23	31909.5
24	39109.5
25	49357.0
26	63034.0
27	77753.5
28	92372.0
29	108426.0
30	123860.0
31	138142.5
32	152883.5
33	167318.0
34	180831.5
35	193519.5
36	206894.0
37	218615.0
38	227048.5
39	233457.0
40	239283.5
41	244281.5
42	248455.0
43	257565.0
44	260945.5
45	257581.5
46	254153.5
47	248762.5
48	243918.5
49	237741.0
50	229612.0
51	220945.5
52	211195.5
53	200052.0
54	188534.5
55	174559.5
56	158216.0
57	141949.5
58	126719.0
59	110534.5
60	93133.0
61	75816.0
62	59624.5
63	46122.0
64	36016.5
65	27485.5
66	19764.0
67	14945.5
68	11514.0
69	8437.5
70	6285.0
71	4638.0
72	3342.0
73	2435.5
74	1812.5
75	1373.5
76	908.0
77	531.0
78	409.0
79	283.0
80	162.0
81	112.5
82	88.5
83	71.5
84	55.0
85	41.5
86	28.5
87	15.5
88	9.0
89	8.5
90	7.5
91	5.0
92	2.5
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.644628973431066E-4
3	0.0
4	0.0
5	0.00512396863602269
6	3.1404969059493904E-4
7	0.0
8	1.6528931083944162E-5
9	1.8181824192338577E-4
10-11	0.0
12-13	0.0
14-15	8.264465541972081E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	9.918590687286425E-5
26-27	3.306694874978414E-5
28-29	6.20287264129565E-4
30-31	2.4017293113587801E-4
32-33	0.00142863514759753
34-35	2.50172076693419E-4
36-37	0.0012998396249484677
38-39	7.01613914880538E-4
40-41	7.41929913206992E-4
42-43	9.465669008100289E-5
44-45	7.828753498039289E-5
46-47	5.197095440253441E-4
48-49	0.0016231755217283002
50-51	0.0016330410580806841
52-53	0.0017510914680804296
54-55	0.0012722621678738868
56-57	0.0029657984680251954
58-59	3.869034868308433E-4
60-61	9.547920295870953E-6
62-63	0.002115484798116581
64-65	0.002336457277732037
66-67	0.003129244223152743
68-69	7.329717607066088E-4
70-71	0.0047763818205001085
72-73	0.0011370165986851334
74-75	0.003916583884325475
76-77	3.289653805322957E-4
78-79	0.0011081250309306744
80-81	1.0910591412971776E-5
82-83	2.2129940370875672E-5
84-85	9.196748164435615E-4
86-87	0.0
88-89	1.1531813448008762E-5
90-91	0.0031246130804495416
92-93	5.700263067140548E-4
94-95	0.0
96-97	1.2288300235615867E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	42.0
20-21	175.0
22-23	402.0
24-25	734.0
26-27	1985.0
28-29	5422.0
30-31	16564.0
32-33	22101.0
34-35	29821.0
36-37	44618.0
38-39	52356.0
40-41	51499.0
42-43	59068.0
44-45	70615.0
46-47	71547.0
48-49	65983.0
50-51	60631.0
52-53	57675.0
54-55	61948.0
56-57	62770.0
58-59	61864.0
60-61	61057.0
62-63	60855.0
64-65	64107.0
66-67	68160.0
68-69	70885.0
70-71	73389.0
72-73	63808.0
74-75	62287.0
76-77	64058.0
78-79	64358.0
80-81	64857.0
82-83	61238.0
84-85	60080.0
86-87	61702.0
88-89	62998.0
90-91	62584.0
92-93	61837.0
94-95	67839.0
96-97	175491.0
98-99	1249173.0
100-101	2671415.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.89359833822573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.8046136004446	78.90339621454821
2	6.6221284942394005	11.50841147045116
3	1.5117669326313028	3.94088605875228
4	0.5228794384941615	1.817395036313147
5	0.21443084650868827	0.9316333923925847
6	0.11556593159183562	0.6025163784794302
7	0.059896696045215025	0.3643247613557795
8	0.036256875361627236	0.25203922917378796
9	0.02506624236675248	0.1960286396458686
>10	0.08505849135753712	1.18115342576705
>50	0.0014828184697081952	0.09379968406671872
>100	7.67229585937947E-4	0.1296832925434696
>500	2.8800967763792852E-5	0.0210470318840475
>1k	5.760193550157971E-5	0.057685384626427944
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.652893108394416E-5	0.0
4	0.0	1.652893108394416E-5	0.0	1.652893108394416E-5	0.0
5	0.0	1.652893108394416E-5	0.0	3.305786216788832E-5	0.0
6	0.0	1.652893108394416E-5	0.0	3.305786216788832E-5	1.652893108394416E-5
7	0.0	1.652893108394416E-5	0.0	3.305786216788832E-5	1.652893108394416E-5
8	0.0	4.958679325183248E-5	0.0	4.958679325183248E-5	1.652893108394416E-5
9	0.0	6.611572433577663E-5	0.0	6.611572433577663E-5	3.305786216788832E-5
10-11	0.0	6.611572433577663E-5	0.0	7.438018987774873E-5	3.305786216788832E-5
12-13	0.0	6.611572433577663E-5	0.0	8.26446554197208E-5	3.305786216788832E-5
14-15	0.0	9.917358650366496E-5	0.0	1.0743805204563704E-4	5.7851258793804555E-5
16-17	0.0	1.1570251758760912E-4	0.0	1.1570251758760912E-4	6.611572433577663E-5
18-19	0.0	1.239669831295812E-4	0.0	1.1570251758760912E-4	8.26446554197208E-5
20-21	0.0	1.3223144867155327E-4	0.0	1.1570251758760912E-4	8.26446554197208E-5
22-23	0.0	1.8181824192338577E-4	0.0	1.1570251758760912E-4	9.090912096169288E-5
24-25	0.0	2.0661163854930202E-4	0.0	1.4049591421352536E-4	9.917358650366496E-5
26-27	0.0	2.148761040912741E-4	0.0	2.0661163854930202E-4	9.917358650366496E-5
28-29	0.0	2.148761040912741E-4	0.0	4.8760346697635274E-4	9.917358650366496E-5
30-31	0.0	2.809918284270507E-4	0.0	0.0016942154361042764	9.917358650366496E-5
32-33	0.0	2.975207595109949E-4	0.0	0.004446282461580979	9.917358650366496E-5
34-35	0.0	3.388430872208553E-4	0.0	0.008115705162216583	9.917358650366496E-5
36-37	0.0	3.8016541493071567E-4	0.0	0.013206615936071385	9.917358650366496E-5
38-39	0.0	4.13223277098604E-4	0.0	0.020719015113724003	9.917358650366496E-5
40-41	0.0	5.123968636022689E-4	0.0	0.02876860455160481	9.917358650366496E-5
42-43	0.0	5.20661329144241E-4	0.0	0.03607439209070813	1.0743805204563704E-4
44-45	0.0	5.537191913121294E-4	0.0	0.04482646109965656	1.1570251758760912E-4
46-47	0.0	5.785125879380456E-4	0.0	0.05436365433509234	1.1570251758760912E-4
48-49	0.0	5.785125879380456E-4	0.0	0.06388431863944417	1.1570251758760912E-4
50-51	0.0	7.024795710676268E-4	0.0	0.07270250337272838	1.1570251758760912E-4
52-53	0.0	7.107440366095988E-4	0.0	0.08196696924527909	1.239669831295812E-4
54-55	0.0	7.272729676935431E-4	0.0	0.09064465806434978	1.3223144867155327E-4
56-57	0.0	7.438018987774872E-4	0.0	0.09790085881020126	1.3223144867155327E-4
58-59	0.0	7.438018987774872E-4	0.0	0.1053471422635181	1.4049591421352536E-4
60-61	0.0	7.768597609453755E-4	0.0	0.1126611942681634	1.4876037975549745E-4
62-63	0.0	7.933886920293197E-4	0.0	0.11995045287618278	1.4876037975549745E-4
64-65	0.0	9.090912096169288E-4	0.0	0.12690913286252326	1.4876037975549745E-4
66-67	0.0	9.090912096169288E-4	0.0	0.13376037479681813	1.4876037975549745E-4
68-69	0.0	9.25620140700873E-4	0.0	0.14025624471280818	1.4876037975549745E-4
70-71	0.0	9.33884606242845E-4	0.0	0.1463223624206157	1.4876037975549745E-4
72-73	0.0	9.421490717848171E-4	0.0	0.15170252948843949	1.652893108394416E-4
74-75	0.0	9.421490717848171E-4	0.0	0.15580996886279963	1.652893108394416E-4
76-77	0.0	9.504135373267892E-4	0.0	0.15953724282222903	1.652893108394416E-4
78-79	0.0	9.586780028687612E-4	0.0	0.16207443374361447	1.652893108394416E-4
80-81	0.0	9.586780028687612E-4	0.0	0.1628678224356438	1.735537763814137E-4
82-83	0.0	9.586780028687612E-4	0.0	0.16352897967900154	1.8181824192338577E-4
84-85	0.0	9.586780028687612E-4	0.0	0.16381823597297057	1.8181824192338577E-4
86-87	0.0	9.752069339527055E-4	0.0	0.16396699635272607	1.9008270746535784E-4
88	0.0	9.752069339527055E-4	0.0	0.16398352528381002	1.9834717300732993E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11245	0.0	55.20942	1
GTATCAA	22180	0.0	38.960293	1
TGGTATC	4060	0.0	32.955555	2
GTGGTAT	4175	0.0	32.836666	1
ATCAACG	27530	0.0	31.015146	3
TCAACGC	27735	0.0	30.800745	4
TATCAAC	28065	0.0	30.629276	2
CAACGCA	28415	0.0	30.049164	5
AACGCAG	29185	0.0	29.29723	6
ACGCAGA	32805	0.0	26.039211	7
CGCAGAG	33135	0.0	25.767456	8
GCAGAGT	36570	0.0	23.121992	9
CTTATAC	4545	0.0	23.00759	1
GAGTACT	22410	0.0	21.042774	12-13
GTACATG	25085	0.0	20.120913	1
TATACAC	6225	0.0	20.03893	3
TACATGG	24655	0.0	20.020987	2
CAGAGTA	36630	0.0	19.808062	10-11
ACATGGG	24875	0.0	19.347404	3
GTACTTT	24660	0.0	18.93107	14-15
>>END_MODULE