FastQCFastQC Report
Fri 27 May 2016
SRR2140053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5270009
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70800.043.6449431
GTATCAA122200.035.11491
TCAACGC143300.029.5868384
ATCAACG144100.029.33363
CAACGCA145550.029.1294635
AACGCAG150850.028.1044256
TATCAAC152950.027.9713552
CTTATAC55300.027.6685771
ACGCAGA173800.024.417877
CGCAGAG173850.024.3616818
TATACAC75000.023.2504833
GCAGAGT204350.020.7674399
TTATACA81550.019.9153792
GAGTACT118850.019.65147612-13
GTACATG143050.019.4202331
TACATGG143050.019.1513372
AGAGTAC179700.018.8475710-11
ACATGGG144200.018.4949153
CAGAGTA198800.018.3588610-11
ACATCTC85700.018.2020538
Produced by FastQC (version 0.11.5)