##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140053_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5270009
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.544319184274638	33.0	32.0	33.0	27.0	34.0
2	32.00303092461512	33.0	32.0	33.0	30.0	34.0
3	32.69153297461162	33.0	33.0	34.0	31.0	34.0
4	32.687354803378895	33.0	33.0	34.0	31.0	34.0
5	33.20660097544425	34.0	33.0	34.0	33.0	34.0
6	37.26947240507559	38.0	38.0	38.0	36.0	38.0
7	37.36528343689736	38.0	38.0	38.0	37.0	38.0
8	37.488013967338574	38.0	38.0	38.0	37.0	38.0
9	37.5074253193875	38.0	38.0	38.0	37.0	38.0
10-11	37.524694739610496	38.0	38.0	38.0	37.0	38.0
12-13	37.54764062072759	38.0	38.0	38.0	37.5	38.0
14-15	37.51925822138065	38.0	38.0	38.0	37.0	38.0
16-17	37.54407022834306	38.0	38.0	38.0	38.0	38.0
18-19	37.46023536202689	38.0	38.0	38.0	37.0	38.0
20-21	37.53758439544326	38.0	38.0	38.0	37.5	38.0
22-23	37.546463711895996	38.0	38.0	38.0	38.0	38.0
24-25	37.54951372458949	38.0	38.0	38.0	38.0	38.0
26-27	37.52472811537402	38.0	38.0	38.0	38.0	38.0
28-29	37.45657977326266	38.0	38.0	38.0	37.0	38.0
30-31	37.49426185682819	38.0	38.0	38.0	37.0	38.0
32-33	37.482919347625234	38.0	38.0	38.0	37.0	38.0
34-35	37.45596291318955	38.0	38.0	38.0	37.0	38.0
36-37	37.43981701448659	38.0	38.0	38.0	37.0	38.0
38-39	37.4154245506269	38.0	38.0	38.0	37.0	38.0
40-41	37.41624795934162	38.0	38.0	38.0	37.0	38.0
42-43	37.356010556843515	38.0	38.0	38.0	37.0	38.0
44-45	37.36801235699359	38.0	38.0	38.0	37.0	38.0
46-47	37.353482970463986	38.0	38.0	38.0	37.0	38.0
48-49	37.32243080810828	38.0	38.0	38.0	37.0	38.0
50-51	37.29442061020221	38.0	38.0	38.0	37.0	38.0
52-53	37.278704182395224	38.0	38.0	38.0	37.0	38.0
54-55	37.26770294958921	38.0	38.0	38.0	37.0	38.0
56-57	37.27267742434739	38.0	38.0	38.0	37.0	38.0
58-59	37.268394076531294	38.0	38.0	38.0	37.0	38.0
60-61	37.26667722020556	38.0	38.0	38.0	37.0	38.0
62-63	37.26906252253407	38.0	38.0	38.0	37.0	38.0
64-65	37.27204602619453	38.0	38.0	38.0	37.0	38.0
66-67	37.2726088979541	38.0	38.0	38.0	37.0	38.0
68-69	37.2734276583341	38.0	38.0	38.0	37.0	38.0
70-71	37.27721364248414	38.0	38.0	38.0	37.0	38.0
72-73	37.25036833707073	38.0	38.0	38.0	37.0	38.0
74-75	37.248179707453446	38.0	38.0	38.0	37.0	38.0
76-77	37.24611892562213	38.0	38.0	38.0	37.0	38.0
78-79	37.23279878563651	38.0	38.0	38.0	37.0	38.0
80-81	37.22797483267983	38.0	38.0	38.0	37.0	38.0
82-83	37.23104026554979	38.0	38.0	38.0	37.0	38.0
84-85	37.21709542699023	38.0	38.0	38.0	37.0	38.0
86-87	37.20350013251412	38.0	38.0	38.0	37.0	38.0
88-89	37.18855921567534	38.0	38.0	38.0	36.5	38.0
90-91	37.18080856857482	38.0	38.0	38.0	36.0	38.0
92-93	37.17836001678434	38.0	38.0	38.0	36.0	38.0
94-95	37.14313811459044	38.0	38.0	38.0	36.0	38.0
96-97	37.15347470519852	38.0	38.0	38.0	36.0	38.0
98-99	37.148104590580765	38.0	38.0	38.0	36.0	38.0
100	35.6729144777833	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	19.0
20	49.0
21	136.0
22	381.0
23	860.0
24	1817.0
25	3550.0
26	6008.0
27	9977.0
28	15501.0
29	22521.0
30	31518.0
31	41867.0
32	54287.0
33	71360.0
34	102700.0
35	180425.0
36	532131.0
37	4194897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.81144832959488	20.85028697294445	14.340772473064087	23.99749222439658
2	14.270978554224687	20.884521770349046	42.35280187491852	22.491697800507744
3	17.770121455200552	26.354262393100274	30.72156043756282	25.15405571413635
4	12.216677428824125	18.34640130595602	39.321564725980544	30.115356539239308
5	13.092448022918154	37.699215907896225	35.96615943648907	13.242176632696554
6	29.357699458481356	38.24611565022655	19.049205748621347	13.346979142670751
7	25.90344722371442	32.898577592561985	23.37047242234311	17.827502761380483
8	23.858345485440715	36.949338757743114	21.613110423993906	17.579205332822266
9	25.135123339658445	17.133074003795066	21.569620493358634	36.16218216318786
10-11	23.7404623028158	26.975162661012536	29.303042935979807	19.98133210019186
12-13	24.673591639027563	24.423535140072815	29.311781061474466	21.591092159425155
14-15	21.87282792116674	25.929082853558693	26.87963151486079	25.318457710413778
16-17	21.466358027092554	29.135775669453317	27.586404501396487	21.811461802057643
18-19	21.51128204904394	28.14120431293381	29.280822860074814	21.06669077794744
20-21	22.229413959731772	27.284598211003114	29.16924141306067	21.316746416204445
22-23	21.96990304923216	27.414719882104162	28.97364033287053	21.641736735793145
24-25	21.797767052640047	27.497975119974615	29.111018839164938	21.5932389882204
26-27	21.77536321278433	27.667201245029844	29.032425815390162	21.52500972679566
28-29	21.626941033881522	27.582615444432967	29.065454077386192	21.724989444299315
30-31	22.16137498375366	27.41786382869404	29.005131183014726	21.415630004537576
32-33	21.94491954908804	27.5722664623759	29.00191789125629	21.48089609727977
34-35	21.905774747055045	27.62519083838339	29.070161150716086	21.39887326384548
36-37	21.847657262125814	27.47098487856346	29.091415891948273	21.58994196736245
38-39	21.626687512152458	27.43776473575949	29.259166985034145	21.67638076705391
40-41	21.869782648180664	27.34077132651208	29.171069806865653	21.618376218441608
42-43	21.907225185711603	27.37177854128751	29.0153537705922	21.705642502408683
44-45	21.806005430997445	27.574184906193423	28.869080135728797	21.750729527080335
46-47	22.014066589193103	27.38236906589585	28.573958172390597	22.029606172520456
48-49	21.94416891030183	27.456965115049087	28.633810800184413	21.96505517446467
50-51	21.786080085841096	27.5972286680702	28.695705079115907	21.9209861669728
52-53	21.93368881479227	27.620514342405723	28.676455320801843	21.76934152200016
54-55	21.907430415073602	27.516117864957955	28.55497266066876	22.021479059299683
56-57	22.042003326037083	27.742271097420517	28.29268262587265	21.923042950669746
58-59	21.79363187842255	27.94771915814282	28.602945231351505	21.65570373208313
60-61	21.987997865683333	28.146201736823706	28.255091604724967	21.61070879276799
62-63	21.808861112592886	28.318183744541475	28.205676673316027	21.66727846954961
64-65	21.793188002871965	28.265864705538895	28.1819306263352	21.759016665253938
66-67	21.84353606348763	28.289742860057665	28.041043594052866	21.825677482401833
68-69	21.854107644728682	28.155724371049423	28.136396755044117	21.853771229177777
70-71	21.881670711025883	27.898071398415215	28.187503681385152	22.032754209173756
72-73	21.978433020915446	27.713326157215583	28.23954766039544	22.068693161473526
74-75	21.931725493197437	27.79665495413916	28.16798805656123	22.103631496102167
76-77	22.069893911370766	27.756707753873112	28.01636128297235	22.15703705178377
78-79	22.025178421211596	27.75445384763361	28.04136255820765	22.179005172947143
80-81	22.106139117262757	27.726043974860694	27.986423261380374	22.181393646496176
82-83	22.075487265352127	27.755910742095818	28.075960861217297	22.09264113133475
84-85	22.245439933731664	27.732768703272704	27.95836578897206	22.063425574023565
86-87	22.06386047033369	27.780842239151887	28.026152306133273	22.12914498438115
88-89	22.137800664759737	27.758677280348483	27.973969059355124	22.129552995536656
90-91	22.199481881002505	27.76077948394377	27.961753838873488	22.077984796180235
92-93	22.28179978806661	27.761434279618403	27.926105123718354	22.030660808596632
94-95	22.171636477428645	27.843768198129048	27.93743606439737	22.047159260044932
96-97	22.242758993746044	27.916674851155626	28.022670278922345	21.817895876175985
98-99	23.299302846404565	29.80713035698017	28.26152166316073	18.632045133454533
100	22.153907088743566	30.72745156370102	22.2587442128933	24.859897134662113
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	711.0
1	701.0
2	1087.0
3	1650.5
4	2090.5
5	2737.0
6	3336.5
7	4221.0
8	5498.0
9	6452.5
10	6766.0
11	7007.5
12	7837.0
13	9377.0
14	12045.0
15	14813.0
16	16633.5
17	17609.5
18	17302.0
19	16120.5
20	15235.5
21	15153.0
22	16344.0
23	19644.0
24	25268.0
25	33104.0
26	43585.5
27	55215.0
28	66690.5
29	79217.0
30	91580.0
31	103743.5
32	117032.0
33	130128.5
34	143333.5
35	156391.0
36	169138.0
37	180910.5
38	189852.0
39	196930.0
40	204479.0
41	211082.5
42	214833.0
43	218629.5
44	228590.0
45	239376.5
46	236130.0
47	230253.0
48	225347.0
49	215091.5
50	207190.5
51	198376.0
52	187202.5
53	175129.5
54	163342.5
55	150955.0
56	137701.0
57	124482.5
58	110567.0
59	95892.5
60	79928.0
61	64365.0
62	50261.0
63	38509.5
64	28932.5
65	20991.0
66	15188.0
67	11520.0
68	8748.5
69	6219.0
70	4391.0
71	3166.5
72	2239.0
73	1506.0
74	971.0
75	626.0
76	389.0
77	221.0
78	126.5
79	80.0
80	54.0
81	35.5
82	21.5
83	15.5
84	12.5
85	11.0
86	9.5
87	5.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.7950599325352194E-4
3	0.0
4	0.0
5	0.009013267339771147
6	2.846294949401415E-4
7	0.0
8	7.590119865070438E-5
9	1.7077769696408488E-4
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	7.590922167997735E-5
26-27	1.8979109883542284E-5
28-29	6.075186508225802E-4
30-31	2.7552195496698156E-4
32-33	0.0014373506904375914
34-35	2.767494837906396E-4
36-37	0.00125479576235356
38-39	7.030197877920325E-4
40-41	7.55579445642985E-4
42-43	7.796655429737028E-5
44-45	7.853079099059427E-5
46-47	4.9488873960845E-4
48-49	0.0014669067337704982
50-51	0.0015285434881181386
52-53	0.0015496577180854575
54-55	0.0015508252890223096
56-57	0.002868786634868035
58-59	3.835353856484583E-4
60-61	4.180834032490725E-5
62-63	0.0019713650269091326
64-65	0.0026268592207650777
66-67	0.003017504425046747
68-69	6.945305071440059E-4
70-71	0.004716338042103298
72-73	0.001109925464065386
74-75	0.004325196995355119
76-77	2.488505085995383E-4
78-79	9.252823110582087E-4
80-81	2.3077127220740798E-5
82-83	4.6613744528711736E-5
84-85	8.70705626899815E-4
86-87	0.0
88-89	0.0
90-91	0.0027300606983495265
92-93	5.518429469688738E-4
94-95	1.239812768434962E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	38.0
20-21	180.0
22-23	252.0
24-25	419.0
26-27	1209.0
28-29	2982.0
30-31	9433.0
32-33	12346.0
34-35	16989.0
36-37	26725.0
38-39	30328.0
40-41	30543.0
42-43	35021.0
44-45	41348.0
46-47	41636.0
48-49	39279.0
50-51	35984.0
52-53	35360.0
54-55	38043.0
56-57	38543.0
58-59	39334.0
60-61	41122.0
62-63	40959.0
64-65	43546.0
66-67	47565.0
68-69	49981.0
70-71	54621.0
72-73	43785.0
74-75	41590.0
76-77	42900.0
78-79	43661.0
80-81	43311.0
82-83	41215.0
84-85	41497.0
86-87	42688.0
88-89	43786.0
90-91	43802.0
92-93	43307.0
94-95	50727.0
96-97	159629.0
98-99	345103.0
100-101	3489222.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.64286147505942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.83689229200756	79.61205167973311
2	6.751456778775674	11.834339824341749
3	1.4782185242990464	3.886659040650243
4	0.49034386419317466	1.7190055745851103
5	0.17977809693294197	0.7878133422871818
6	0.10014118984074194	0.5265996257495864
7	0.05155822108714073	0.3163097020048534
8	0.033585487672656074	0.235482259493353
9	0.019185723251685965	0.15133425167217054
>10	0.05687939422946446	0.740115411376679
>50	0.0014308676204823725	0.08566072002552158
>100	4.989879953468467E-4	0.08370225257250889
>500	3.057209402087482E-5	0.02092631550795013
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.89752996626761E-5	0.0	0.0	0.0
3	0.0	1.89752996626761E-5	0.0	0.0	0.0
4	0.0	9.487649831338049E-5	0.0	1.89752996626761E-5	0.0
5	0.0	9.487649831338049E-5	0.0	1.89752996626761E-5	0.0
6	0.0	9.487649831338049E-5	0.0	1.89752996626761E-5	0.0
7	0.0	9.487649831338049E-5	0.0	1.89752996626761E-5	0.0
8	0.0	9.487649831338049E-5	0.0	1.89752996626761E-5	0.0
9	0.0	2.4667889561478927E-4	0.0	1.89752996626761E-5	0.0
10-11	0.0	2.4667889561478927E-4	0.0	5.6925898988028294E-5	0.0
12-13	0.0	2.4667889561478927E-4	0.0	8.538884848204244E-5	0.0
14-15	0.0	2.941171447714795E-4	0.0	9.487649831338049E-5	0.0
16-17	0.0	3.225800942654937E-4	0.0	1.1385179797605659E-4	0.0
18-19	0.0	3.6053069359084586E-4	0.0	1.4231474747007075E-4	0.0
20-21	0.0	3.9848129291619803E-4	0.0	1.7077769696408488E-4	0.0
22-23	0.0	4.269442424102122E-4	0.0	2.087282962894371E-4	0.0
24-25	0.0	5.028454410609166E-4	0.0	2.5616654544612734E-4	0.0
26-27	0.0	5.313083905549307E-4	0.0	2.846294949401415E-4	0.0
28-29	0.0	5.597713400489449E-4	0.0	6.166972390369732E-4	0.0
30-31	0.0	6.261848888683112E-4	0.0	0.0016223881211588063	0.0
32-33	0.0	6.261848888683112E-4	0.0	0.0038045475823665575	0.0
34-35	0.0	6.925984376876775E-4	0.0	0.006916496727045437	0.0
36-37	0.0	7.590119865070439E-4	0.0	0.01109106265283418	0.0
38-39	0.0	7.7798728616972E-4	0.0	0.022400341251789135	0.0
40-41	0.0	9.108143838084527E-4	0.0	0.034202977641973664	0.0
42-43	0.0	9.487649831338049E-4	0.0	0.04196387520400819	1.89752996626761E-5
44-45	0.0	0.0010815920807725377	0.0	0.05048378475254976	3.79505993253522E-5
46-47	0.0	0.001157493279423242	0.0	0.06005682343236985	3.79505993253522E-5
48-49	0.0	0.001157493279423242	0.0	0.06973422626033465	3.79505993253522E-5
50-51	0.0	0.001347246276050003	0.0	0.07937367848897411	3.79505993253522E-5
52-53	0.0	0.001347246276050003	0.0	0.10519906132987628	3.79505993253522E-5
54-55	0.0	0.001366221575712679	0.0	0.13724834246013623	3.79505993253522E-5
56-57	0.0	0.0014041721750380314	0.0	0.18238109270781133	3.79505993253522E-5
58-59	0.0	0.0014231474747007074	0.0	0.21528426232289166	3.79505993253522E-5
60-61	0.0	0.0014421227743633834	0.0	0.22920264462546458	3.79505993253522E-5
62-63	0.0	0.0014516104241947213	0.0	0.24191609539945758	3.79505993253522E-5
64-65	0.0	0.0016698263703154967	0.0	0.24782690124438117	3.79505993253522E-5
66-67	0.0	0.0017077769696408488	0.0	0.2539084847862688	3.79505993253522E-5
68-69	0.0	0.0017552152187975392	0.0	0.2604265002203981	3.79505993253522E-5
70-71	0.0	0.0018121411177855675	0.0	0.265587781728646	3.79505993253522E-5
72-73	0.0	0.0018500917171109196	0.0	0.26980978590359145	5.6925898988028294E-5
74-75	0.0	0.0019165052659302858	0.0	0.2730640497957404	5.6925898988028294E-5
76-77	0.0	0.0019924064645809904	0.0	0.27615702364075656	5.6925898988028294E-5
78-79	0.0	0.002011381764243666	0.0	0.2782822572029763	5.6925898988028294E-5
80-81	0.0	0.002011381764243666	0.0	0.27887997914235063	6.641354881936634E-5
82-83	0.0	0.002011381764243666	0.0	0.27936384928374886	7.59011986507044E-5
84-85	0.0	0.0020303570639063425	0.0	0.2795725775800383	7.59011986507044E-5
86-87	0.0	0.0020493323635690187	0.0	0.2796959170278457	7.59011986507044E-5
88	0.0	0.002144208861882399	0.0	0.2796959170278457	7.59011986507044E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7080	0.0	43.644943	1
GTATCAA	12220	0.0	35.1149	1
TCAACGC	14330	0.0	29.586838	4
ATCAACG	14410	0.0	29.3336	3
CAACGCA	14555	0.0	29.129463	5
AACGCAG	15085	0.0	28.104425	6
TATCAAC	15295	0.0	27.971355	2
CTTATAC	5530	0.0	27.668577	1
ACGCAGA	17380	0.0	24.41787	7
CGCAGAG	17385	0.0	24.361681	8
TATACAC	7500	0.0	23.250483	3
GCAGAGT	20435	0.0	20.767439	9
TTATACA	8155	0.0	19.915379	2
GAGTACT	11885	0.0	19.651476	12-13
GTACATG	14305	0.0	19.420233	1
TACATGG	14305	0.0	19.151337	2
AGAGTAC	17970	0.0	18.84757	10-11
ACATGGG	14420	0.0	18.494915	3
CAGAGTA	19880	0.0	18.35886	10-11
ACATCTC	8570	0.0	18.202053	8
>>END_MODULE