FastQCFastQC Report
Fri 27 May 2016
SRR2140052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8777891
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97950.045.7599141
GTATCAA172850.036.3630941
TCAACGC200450.030.8204464
ATCAACG202300.030.5810413
CAACGCA206650.029.8749835
AACGCAG213600.028.9813426
TATCAAC216950.028.9318922
ACGCAGA245650.025.1124747
CGCAGAG249600.024.6978658
GCAGAGT303150.020.3775869
GTGGTAT48800.019.8828091
GTACATG211200.019.6775281
GAGTACT179950.019.10865812-13
TACATGG213650.018.8892232
TATACCG5950.018.7603345
AGAGTAC268350.018.52492510-11
TGGTATC48900.018.4373952
ACATGGG216550.018.2395363
CAGAGTA293050.018.00361410-11
GTACTTT204400.016.68653914-15
Produced by FastQC (version 0.11.5)