##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140052_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8777891
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53187605086461	33.0	32.0	33.0	27.0	34.0
2	32.04539142716627	33.0	32.0	33.0	30.0	34.0
3	32.72037668273621	33.0	33.0	34.0	31.0	34.0
4	32.73080276344284	33.0	33.0	34.0	31.0	34.0
5	33.23763339052627	34.0	33.0	34.0	33.0	34.0
6	37.3090180773491	38.0	38.0	38.0	37.0	38.0
7	37.40236874666136	38.0	38.0	38.0	37.0	38.0
8	37.51858504508657	38.0	38.0	38.0	37.0	38.0
9	37.53647840922153	38.0	38.0	38.0	37.0	38.0
10-11	37.553620112165895	38.0	38.0	38.0	37.5	38.0
12-13	37.57588958441157	38.0	38.0	38.0	38.0	38.0
14-15	37.546542102197435	38.0	38.0	38.0	38.0	38.0
16-17	37.570486976883174	38.0	38.0	38.0	38.0	38.0
18-19	37.484952194097644	38.0	38.0	38.0	37.5	38.0
20-21	37.56237218604943	38.0	38.0	38.0	38.0	38.0
22-23	37.57077944241314	38.0	38.0	38.0	38.0	38.0
24-25	37.572638528513856	38.0	38.0	38.0	38.0	38.0
26-27	37.550343468750604	38.0	38.0	38.0	38.0	38.0
28-29	37.48356706360264	38.0	38.0	38.0	37.5	38.0
30-31	37.52123213401588	38.0	38.0	38.0	38.0	38.0
32-33	37.512495016500594	38.0	38.0	38.0	38.0	38.0
34-35	37.493540248610095	38.0	38.0	38.0	37.5	38.0
36-37	37.476457194586004	38.0	38.0	38.0	37.0	38.0
38-39	37.467669727155595	38.0	38.0	38.0	37.0	38.0
40-41	37.44839191592824	38.0	38.0	38.0	37.0	38.0
42-43	37.386126188331254	38.0	38.0	38.0	37.0	38.0
44-45	37.39977679024099	38.0	38.0	38.0	37.0	38.0
46-47	37.38499406416434	38.0	38.0	38.0	37.0	38.0
48-49	37.3556366089464	38.0	38.0	38.0	37.0	38.0
50-51	37.32865345080232	38.0	38.0	38.0	37.0	38.0
52-53	37.31196409678063	38.0	38.0	38.0	37.0	38.0
54-55	37.30279851515233	38.0	38.0	38.0	37.0	38.0
56-57	37.30551848194835	38.0	38.0	38.0	37.0	38.0
58-59	37.301498760895456	38.0	38.0	38.0	37.0	38.0
60-61	37.30262450933269	38.0	38.0	38.0	37.0	38.0
62-63	37.30500510357082	38.0	38.0	38.0	37.0	38.0
64-65	37.30622899743682	38.0	38.0	38.0	37.0	38.0
66-67	37.30926480499757	38.0	38.0	38.0	37.0	38.0
68-69	37.310328725926794	38.0	38.0	38.0	37.0	38.0
70-71	37.31752154712548	38.0	38.0	38.0	37.0	38.0
72-73	37.29507808022451	38.0	38.0	38.0	37.0	38.0
74-75	37.29364687775565	38.0	38.0	38.0	37.0	38.0
76-77	37.290201674775155	38.0	38.0	38.0	37.0	38.0
78-79	37.274334598736914	38.0	38.0	38.0	37.0	38.0
80-81	37.268390717709465	38.0	38.0	38.0	37.0	38.0
82-83	37.26960175312334	38.0	38.0	38.0	37.0	38.0
84-85	37.2553137966232	38.0	38.0	38.0	37.0	38.0
86-87	37.242873737808395	38.0	38.0	38.0	37.0	38.0
88-89	37.224259751333705	38.0	38.0	38.0	37.0	38.0
90-91	37.21497863777344	38.0	38.0	38.0	36.5	38.0
92-93	37.211908694447864	38.0	38.0	38.0	36.5	38.0
94-95	37.173772161248976	38.0	38.0	38.0	36.0	38.0
96-97	37.1858350906823	38.0	38.0	38.0	36.0	38.0
98-99	37.177088329191236	38.0	38.0	38.0	36.0	38.0
100	35.69858105818446	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	3.0
19	24.0
20	87.0
21	255.0
22	685.0
23	1519.0
24	2994.0
25	5797.0
26	9729.0
27	15525.0
28	23649.0
29	34801.0
30	48569.0
31	64419.0
32	83382.0
33	110671.0
34	158809.0
35	282968.0
36	857312.0
37	7076690.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.863733669055584	20.890416616018587	14.390039703158767	23.855810011767066
2	14.355405769804547	20.99511373543767	42.25629426543179	22.393186229325995
3	17.77551122473496	26.253960091324895	30.60558623933699	25.36494244460315
4	12.31825503415342	18.274389599961996	39.5876070914984	29.819748274386182
5	13.171560635424107	37.69055488525292	35.97214579253127	13.165738686791695
6	29.538001639354533	38.265246196532296	19.127804147828993	13.06894801628418
7	26.117571977141207	32.85356357238886	23.585323627281312	17.443540823188624
8	23.831435476769176	37.09774051494181	21.602869028245415	17.467954980043597
9	25.22692288062528	17.23912128133106	21.800682443307444	35.73327339473622
10-11	23.70176389750112	27.086341126815082	29.413175670556857	19.798719305126937
12-13	24.56437428990631	24.612375569484744	29.450303039762055	21.37294710084689
14-15	21.7852797320723	26.091752910337622	27.088211000125828	25.034756357464243
16-17	21.380898897012962	29.17462178557469	27.82479869025487	21.619680627157482
18-19	21.429133717882802	28.352852638521032	29.42038696994529	20.79762667365088
20-21	22.151646552031043	27.513905087400765	29.336409270532947	20.998039090035245
22-23	21.906808355116134	27.44120011374449	29.13721659575864	21.514774935380736
24-25	21.679060369047633	27.53412272417142	29.250913071513153	21.53590383526779
26-27	21.65056088831953	27.69156802555154	29.178823220692678	21.479047865436254
28-29	21.562501834290888	27.742581871205225	29.192946771856043	21.501969522647844
30-31	21.865098080460275	27.519650056770413	29.15375498897758	21.461496873791734
32-33	21.65986424412651	27.67215089126364	29.068670409827675	21.599314454782174
34-35	21.80122110082388	27.71099162598431	29.0899315894819	21.39785568370991
36-37	21.7555934554477	27.62202414440208	29.073434263979927	21.548948136170303
38-39	21.624302306776126	27.727605814758526	29.151349099950743	21.496742778514605
40-41	21.881858745200784	27.59832775598933	29.068537530588678	21.4512759682212
42-43	21.81170512029936	27.652809441146776	28.873478001913984	21.66200743663988
44-45	21.841642719227654	27.70941163063729	28.799671611632462	21.649274038502593
46-47	21.87026915026599	27.755703736115244	28.602769069374567	21.771258044244195
48-49	21.820515369211332	27.79482871404131	28.66857258679017	21.71608332995719
50-51	21.79651790089043	27.90769553960742	28.5506416376548	21.74514492184735
52-53	21.954752571056062	27.85831204639697	28.442804307040763	21.744131075506203
54-55	21.912326520515858	27.79618274333493	28.455507391574308	21.835983344574903
56-57	21.905399960148987	27.88702213309449	28.41778533916763	21.789792567588893
58-59	21.87201641545958	27.919431355315115	28.471914384364645	21.736637844860656
60-61	21.964602832254602	27.900309209263348	28.463849231788657	21.671238726693396
62-63	21.909773126433286	27.970997733519003	28.40661024010855	21.712618899939162
64-65	21.886070043528946	27.92637897111122	28.359255063418132	21.828295921941702
66-67	21.969783555440515	27.949302140224948	28.240142630694958	21.840771673639576
68-69	21.944147886814804	27.915921940484367	28.30111004591717	21.838820126783656
70-71	21.926327297322963	27.759996999677778	28.329082985941096	21.98459271705816
72-73	21.99841396765379	27.774009216955154	28.255275242008654	21.9723015733824
74-75	21.900343662994207	27.86679228611965	28.220038684162663	22.012825366723476
76-77	22.030648519080007	27.771066140111955	28.140974120835445	22.057311219972597
78-79	21.983067251292017	27.820861694019143	28.10623189909617	22.089839155592667
80-81	21.99286782295113	27.827154593246135	28.083613992945995	22.096363590856743
82-83	22.0384421848228	27.82596251546347	28.145540939741547	21.990054359972184
84-85	22.174691759396495	27.798031609862132	28.048007988285935	21.979268642455434
86-87	21.993299977866034	27.829829400550587	28.11129129206679	22.06557932951658
88-89	22.085045566870313	27.802992729669736	28.05033547887103	22.061626224588927
90-91	22.079588005945954	27.81759207236656	28.090815958677307	22.012003963010176
92-93	22.110218437454073	27.794794520946624	28.103067670354587	21.99191937124472
94-95	22.057695232598128	27.900419776599655	28.02660428708142	22.015280703720798
96-97	22.10751576511603	27.993325865021195	28.1121537258531	21.787004644009667
98-99	23.226298845045676	29.8723821939417	28.3568759127547	18.54444304825793
100	22.00789315883225	30.79235145235345	22.347561120539257	24.852194268275042
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1406.0
1	1289.0
2	1700.0
3	2509.5
4	3224.5
5	4254.5
6	5159.5
7	6509.0
8	8400.5
9	9815.0
10	10388.0
11	10852.5
12	12116.0
13	14588.5
14	18890.0
15	23186.0
16	26038.0
17	27456.0
18	27025.0
19	25445.0
20	24209.0
21	24250.5
22	26808.0
23	32770.0
24	42041.5
25	55146.5
26	72284.5
27	92144.5
28	113213.5
29	135495.5
30	157782.5
31	180323.5
32	203160.0
33	225157.5
34	246935.0
35	266795.0
36	286912.0
37	306607.5
38	322348.0
39	334885.0
40	346987.5
41	357169.5
42	363700.0
43	369878.5
44	373754.5
45	375469.5
46	374993.5
47	372492.0
48	367532.5
49	357203.0
50	344827.0
51	329144.5
52	309046.0
53	288591.0
54	268407.5
55	246802.0
56	225219.5
57	201620.5
58	176749.0
59	152475.5
60	126916.5
61	102616.0
62	80662.5
63	61744.0
64	46474.5
65	34447.5
66	25264.5
67	18880.0
68	14131.5
69	10372.0
70	7596.0
71	5617.0
72	4013.0
73	2742.0
74	1891.5
75	1237.5
76	770.5
77	469.0
78	285.0
79	181.0
80	115.5
81	70.0
82	46.5
83	39.0
84	31.5
85	25.5
86	21.0
87	16.0
88	10.0
89	4.0
90	1.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.2784516235164006E-4
3	0.0
4	0.0
5	0.008669508427479903
6	5.240438734087721E-4
7	0.0
8	6.835354870549201E-5
9	2.1645290423405803E-4
10-11	0.0
12-13	0.0
14-15	5.6961290587910014E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.0823602060585968E-4
26-27	1.1394320102343782E-5
28-29	6.32559225209548E-4
30-31	2.7377025964086247E-4
32-33	0.001542864372278773
34-35	3.036130004337083E-4
36-37	0.0012993860860702996
38-39	7.109388930681435E-4
40-41	6.510815190685209E-4
42-43	1.2280352733834584E-4
44-45	4.711452120780667E-5
46-47	4.808935072608389E-4
48-49	0.00151998938640482
50-51	0.0016460789224297064
52-53	0.0017124392987330316
54-55	0.001321200876176381
56-57	0.002977640141893492
58-59	3.7908839061068906E-4
60-61	1.879615042308568E-5
62-63	0.002077790716077414
64-65	0.0024069318107940193
66-67	0.0031543666070399296
68-69	7.002781595626581E-4
70-71	0.004902118631532678
72-73	0.0011163534953820618
74-75	0.004071781170351196
76-77	2.959524135423789E-4
78-79	9.642857094351826E-4
80-81	1.3714404520212872E-5
82-83	3.4617118400445424E-5
84-85	9.294794181458843E-4
86-87	0.0
88-89	0.0
90-91	0.00288763118108796
92-93	7.203101786594795E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	48.0
20-21	194.0
22-23	412.0
24-25	680.0
26-27	1751.0
28-29	4808.0
30-31	15517.0
32-33	20145.0
34-35	27806.0
36-37	43715.0
38-39	49517.0
40-41	49738.0
42-43	57327.0
44-45	67264.0
46-47	67391.0
48-49	63993.0
50-51	59400.0
52-53	58223.0
54-55	63489.0
56-57	64589.0
58-59	65243.0
60-61	63699.0
62-63	64012.0
64-65	68182.0
66-67	71534.0
68-69	71777.0
70-71	71509.0
72-73	66157.0
74-75	68621.0
76-77	70334.0
78-79	71344.0
80-81	70674.0
82-83	67480.0
84-85	67534.0
86-87	70416.0
88-89	71859.0
90-91	71750.0
92-93	71532.0
94-95	84599.0
96-97	265802.0
98-99	577662.0
100-101	5890164.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.49452991796484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.75021153711032	76.7315214540315
2	7.167028959545522	12.2548354360957
3	1.8128971755516872	4.6497837544019305
4	0.6148549409815582	2.102669365878253
5	0.2723108750687469	1.164054512777608
6	0.12974343614094885	0.6655412449687147
7	0.08208204456442483	0.49122960703186597
8	0.0440262301802311	0.30112014826551786
9	0.02853337353486764	0.21955026216034867
>10	0.0965191495981838	1.242532523448959
>50	0.0014215405150206117	0.08229117561045794
>100	3.0916471125339067E-4	0.05242425646393283
>500	4.104841503526912E-5	0.023653456607810198
>1k	2.0524082157581216E-5	0.018792802257403624
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.2784516235164006E-5	0.0	0.0	0.0
3	0.0	2.2784516235164006E-5	0.0	0.0	0.0
4	0.0	6.835354870549201E-5	0.0	0.0	0.0
5	0.0	7.974580682307401E-5	0.0	1.1392258117582003E-5	0.0
6	2.2784516235164006E-5	7.974580682307401E-5	0.0	3.417677435274601E-5	2.2784516235164006E-5
7	2.2784516235164006E-5	7.974580682307401E-5	0.0	3.417677435274601E-5	3.417677435274601E-5
8	2.2784516235164006E-5	1.1392258117582002E-4	0.0	3.417677435274601E-5	4.556903247032801E-5
9	2.2784516235164006E-5	2.1645290423405805E-4	0.0	7.974580682307401E-5	5.696129058791001E-5
10-11	6.835354870549201E-5	2.2784516235164006E-4	0.0	1.1392258117582002E-4	5.696129058791001E-5
12-13	6.835354870549201E-5	2.3923742046922205E-4	0.0	1.31010968352193E-4	5.696129058791001E-5
14-15	7.974580682307403E-5	3.0759096917471406E-4	0.0	1.5949161364614802E-4	8.544193588186502E-5
16-17	1.1392258117582003E-4	3.1898322729229605E-4	0.0	1.7088387176373003E-4	9.113806494065602E-5
18-19	1.3670709741098403E-4	3.6455225976262404E-4	0.0	1.8797225894010304E-4	9.683419399944703E-5
20-21	1.3670709741098403E-4	4.101212922329521E-4	0.0	2.0506064611647604E-4	1.1392258117582002E-4
22-23	1.4809935552856604E-4	4.3860193752690707E-4	0.0	2.2214903329284905E-4	1.1392258117582002E-4
24-25	1.8797225894010304E-4	5.012593571736082E-4	0.0	2.8480645293955004E-4	1.1392258117582002E-4
26-27	1.9366838799889403E-4	5.240438734087721E-4	0.0	3.4176774352746007E-4	1.1392258117582002E-4
28-29	1.9366838799889403E-4	5.240438734087721E-4	0.0	5.753090349378911E-4	1.1392258117582002E-4
30-31	2.1645290423405803E-4	5.696129058791001E-4	0.0	0.0014069438775213773	1.2531483929340202E-4
32-33	2.2784516235164004E-4	5.696129058791001E-4	0.0	0.003315147112216363	2.0506064611647604E-4
34-35	2.4493354952801307E-4	6.550548417609652E-4	0.0	0.006009416157024506	2.2214903329284905E-4
36-37	2.6202193670438607E-4	7.291045195252482E-4	0.0	0.0101903748861771	2.3923742046922205E-4
38-39	2.6202193670438607E-4	7.404967776428301E-4	0.0	0.0185465962154235	2.3923742046922205E-4
40-41	2.7341419482196806E-4	7.974580682307402E-4	0.0	0.027096485932668793	2.3923742046922205E-4
42-43	2.7341419482196806E-4	8.316348425834862E-4	0.0	0.03340779692980922	2.3923742046922205E-4
44-45	2.7341419482196806E-4	9.113806494065601E-4	0.0	0.040846941480590274	2.3923742046922205E-4
46-47	2.7341419482196806E-4	9.455574237593062E-4	0.0	0.049624676360187206	2.3923742046922205E-4
48-49	2.7341419482196806E-4	9.512535528180972E-4	0.0	0.05751381510661274	2.3923742046922205E-4
50-51	2.7341419482196806E-4	0.0010366954886999622	0.0	0.06585864417774157	2.6202193670438607E-4
52-53	2.7341419482196806E-4	0.0010480877468175443	0.0	0.08298120812846731	2.6202193670438607E-4
54-55	2.7341419482196806E-4	0.0010480877468175443	0.0	0.10489421661763629	2.6771806576317704E-4
56-57	2.7341419482196806E-4	0.0010480877468175443	0.0	0.13113058706242764	2.848064529395501E-4
58-59	2.7341419482196806E-4	0.0010651761339939172	0.0	0.15315751813277245	2.961987110571321E-4
60-61	2.7341419482196806E-4	0.0010993529083466631	0.0	0.16250486591824848	2.961987110571321E-4
62-63	2.7341419482196806E-4	0.0011221374245818272	0.0	0.17467749371688485	2.961987110571321E-4
64-65	2.7341419482196806E-4	0.0012417561348164383	0.0	0.18133626858661153	2.961987110571321E-4
66-67	2.7341419482196806E-4	0.0012531483929340203	0.0	0.1872944195821069	2.961987110571321E-4
68-69	2.961987110571321E-4	0.0012702367801103933	0.0	0.19365129961171768	3.0189484011592304E-4
70-71	2.961987110571321E-4	0.0012816290382279753	0.0	0.19878351189368837	3.0759096917471406E-4
72-73	2.961987110571321E-4	0.0012987174254043483	0.0	0.20245181900754977	3.0759096917471406E-4
74-75	2.961987110571321E-4	0.0013442864578746762	0.0	0.20531127579506286	3.132870982335051E-4
76-77	2.961987110571321E-4	0.0013955516194037953	0.0	0.20854098097139734	3.303754854098781E-4
78-79	2.961987110571321E-4	0.0014126400065801682	0.0	0.2106599409812676	3.4176774352746007E-4
80-81	2.961987110571321E-4	0.0014126400065801682	0.0	0.21126942679055824	3.4176774352746007E-4
82-83	3.0189484011592304E-4	0.0014297283937565412	0.0	0.2117877745349082	3.5885613070383307E-4
84-85	3.0759096917471406E-4	0.0014411206518741234	0.0	0.212038404213495	3.759445178802061E-4
86-87	3.0759096917471406E-4	0.0014809935552856602	0.0	0.21215802292372962	3.816406469389971E-4
88	3.1898322729229605E-4	0.0015265625877559884	0.0	0.2121694151818472	3.8733677599778806E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9795	0.0	45.759914	1
GTATCAA	17285	0.0	36.363094	1
TCAACGC	20045	0.0	30.820446	4
ATCAACG	20230	0.0	30.581041	3
CAACGCA	20665	0.0	29.874983	5
AACGCAG	21360	0.0	28.981342	6
TATCAAC	21695	0.0	28.931892	2
ACGCAGA	24565	0.0	25.112474	7
CGCAGAG	24960	0.0	24.697865	8
GCAGAGT	30315	0.0	20.377586	9
GTGGTAT	4880	0.0	19.882809	1
GTACATG	21120	0.0	19.677528	1
GAGTACT	17995	0.0	19.108658	12-13
TACATGG	21365	0.0	18.889223	2
TATACCG	595	0.0	18.760334	5
AGAGTAC	26835	0.0	18.524925	10-11
TGGTATC	4890	0.0	18.437395	2
ACATGGG	21655	0.0	18.239536	3
CAGAGTA	29305	0.0	18.003614	10-11
GTACTTT	20440	0.0	16.686539	14-15
>>END_MODULE