FastQCFastQC Report
Fri 27 May 2016
SRR2140051_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140051_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6323287
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72500.049.5959931
CTTATAC62550.041.8949471
GTATCAA130500.037.3303151
TATACAC86050.033.8983083
ATCAACG155300.031.1477073
TCAACGC155650.030.9672664
TTATACA89400.030.8020522
CAACGCA159700.030.2088345
TATCAAC165500.029.4097292
AACGCAG166750.028.9288946
ACGCAGA189400.025.4916157
CGCAGAG193150.025.0189368
ACACATC110500.024.4904176
ACATCTC113700.023.8007768
TACACAT120450.023.5751135
CACATCT125200.021.5116777
GCAGAGT230550.020.867199
ATACACA156550.020.0596544
GTGGTAT37350.020.0132561
CATCTCC134400.019.879359
Produced by FastQC (version 0.11.5)