##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140051_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6323287
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14967389587093	33.0	32.0	33.0	25.0	34.0
2	31.927109112713055	33.0	32.0	33.0	29.0	34.0
3	32.649246981830814	33.0	33.0	34.0	31.0	34.0
4	32.682771792581924	33.0	33.0	34.0	31.0	34.0
5	33.20554341436661	34.0	33.0	34.0	33.0	34.0
6	37.271420069973104	38.0	38.0	38.0	36.0	38.0
7	37.36953502189605	38.0	38.0	38.0	37.0	38.0
8	37.48423913069263	38.0	38.0	38.0	37.0	38.0
9	37.503231468064	38.0	38.0	38.0	37.0	38.0
10-11	37.522660998939315	38.0	38.0	38.0	37.0	38.0
12-13	37.544026390072126	38.0	38.0	38.0	37.5	38.0
14-15	37.51478692964592	38.0	38.0	38.0	37.0	38.0
16-17	37.53962330034996	38.0	38.0	38.0	37.5	38.0
18-19	37.45427987057997	38.0	38.0	38.0	37.0	38.0
20-21	37.53213446032275	38.0	38.0	38.0	37.0	38.0
22-23	37.542463261480236	38.0	38.0	38.0	38.0	38.0
24-25	37.54283797710525	38.0	38.0	38.0	38.0	38.0
26-27	37.520724656020356	38.0	38.0	38.0	37.5	38.0
28-29	37.45088647838929	38.0	38.0	38.0	37.0	38.0
30-31	37.48999531425445	38.0	38.0	38.0	37.0	38.0
32-33	37.47895317329239	38.0	38.0	38.0	37.0	38.0
34-35	37.452819895669684	38.0	38.0	38.0	37.0	38.0
36-37	37.43285793285239	38.0	38.0	38.0	37.0	38.0
38-39	37.40685097493964	38.0	38.0	38.0	37.0	38.0
40-41	37.412498357471065	38.0	38.0	38.0	37.0	38.0
42-43	37.35395578360006	38.0	38.0	38.0	37.0	38.0
44-45	37.364119310023014	38.0	38.0	38.0	37.0	38.0
46-47	37.348836846503474	38.0	38.0	38.0	37.0	38.0
48-49	37.318480579958134	38.0	38.0	38.0	37.0	38.0
50-51	37.29049544061421	38.0	38.0	38.0	37.0	38.0
52-53	37.27379214612016	38.0	38.0	38.0	37.0	38.0
54-55	37.26376403834297	38.0	38.0	38.0	37.0	38.0
56-57	37.26915921422776	38.0	38.0	38.0	37.0	38.0
58-59	37.263723125684734	38.0	38.0	38.0	37.0	38.0
60-61	37.26120528997838	38.0	38.0	38.0	37.0	38.0
62-63	37.26368743446303	38.0	38.0	38.0	37.0	38.0
64-65	37.26385247117711	38.0	38.0	38.0	37.0	38.0
66-67	37.2629615681426	38.0	38.0	38.0	37.0	38.0
68-69	37.26078799427877	38.0	38.0	38.0	37.0	38.0
70-71	37.26480216939563	38.0	38.0	38.0	37.0	38.0
72-73	37.232527682333235	38.0	38.0	38.0	37.0	38.0
74-75	37.23220641603761	38.0	38.0	38.0	37.0	38.0
76-77	37.234312287381854	38.0	38.0	38.0	37.0	38.0
78-79	37.22172811624561	38.0	38.0	38.0	37.0	38.0
80-81	37.2177801322738	38.0	38.0	38.0	37.0	38.0
82-83	37.222067159706455	38.0	38.0	38.0	37.0	38.0
84-85	37.20761680572764	38.0	38.0	38.0	37.0	38.0
86-87	37.19392938704891	38.0	38.0	38.0	36.5	38.0
88-89	37.17938010699966	38.0	38.0	38.0	36.0	38.0
90-91	37.16764337748269	38.0	38.0	38.0	36.0	38.0
92-93	37.16463951612819	38.0	38.0	38.0	36.0	38.0
94-95	37.128801564041694	38.0	38.0	38.0	36.0	38.0
96-97	37.144261923691225	38.0	38.0	38.0	36.0	38.0
98-99	37.13729696662196	38.0	38.0	38.0	36.0	38.0
100	35.657448226742694	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	29.0
20	71.0
21	172.0
22	461.0
23	1043.0
24	2157.0
25	4047.0
26	7102.0
27	11635.0
28	18513.0
29	27241.0
30	38300.0
31	50967.0
32	66651.0
33	87884.0
34	126128.0
35	223135.0
36	659066.0
37	4998681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.511746501463556	20.88796538888714	14.413611148758548	24.18667696089075
2	14.288883986358314	20.65225797115889	42.496068091405306	22.562789951077487
3	17.695796505836284	26.11842543284845	30.73407865244769	25.451699408867572
4	12.235772312722798	18.420277301979176	38.974681996879156	30.369268388418874
5	13.14563443077855	37.59025988044693	35.994031045207635	13.270074643566884
6	29.602583098367475	38.12367957725597	18.87373267487541	13.400004649501152
7	26.095288731952227	32.7266024774773	23.054243781754646	18.123865008815827
8	23.85216144289764	37.04748930775756	21.511934636413148	17.58841461293165
9	25.033099931111657	17.129190628402114	21.34580239736491	36.491907043121316
10-11	23.78370616421491	26.92450935723778	29.238780400130505	20.053004078416812
12-13	24.704184706466748	24.291400975473675	29.20114174795482	21.803272570104756
14-15	21.896872773359235	25.813483225850987	26.647780390782543	25.64186361000723
16-17	21.602071833842114	29.043264998093555	27.392319848838113	21.962343319226218
18-19	21.65656089941829	28.007047600401503	29.08639130249821	21.250000197682
20-21	22.29126241843836	27.183905132971653	28.961934435658733	21.56289801293125
22-23	22.002169050737837	27.361171461365313	28.846904002305852	21.789755485590995
24-25	21.903184682549895	27.463811432036017	29.02943569091263	21.603568194501456
26-27	21.990493853034334	27.59001775482524	28.955011882683706	21.464476509456716
28-29	21.72263356007413	27.39524333222107	29.159252222118326	21.722870885586477
30-31	22.20312837226816	27.417681227027014	28.87040783671774	21.508782563987094
32-33	21.96507104023809	27.267302265012106	28.949443290308423	21.818183404441385
34-35	21.769100219449363	27.58197233494314	29.231356423480985	21.417571022126513
36-37	22.039251807541966	27.398212557569266	28.90271387175724	21.65982176313153
38-39	21.66708200952819	27.337057307051595	29.16128645315157	21.83457423026865
40-41	21.951037226977373	27.228738973378896	29.096074053261557	21.72414974638217
42-43	22.09432464017505	27.155289067201217	28.974525037226627	21.77586125539711
44-45	21.898436226527707	27.44347716496973	28.827782488881294	21.83030411962127
46-47	22.13969749005172	27.237354117610767	28.463969234882214	22.158979157455295
48-49	21.97613563714788	27.296077396284574	28.56538767113476	22.16239929543278
50-51	21.839275477011643	27.42618027808027	28.66005062229148	22.07449362261661
52-53	22.03470352310613	27.39734169503113	28.79057220703407	21.777382574828668
54-55	21.973776527054742	27.369434428999114	28.549853917532257	22.10693512641389
56-57	22.196166641812106	27.66526775869759	28.167873051319948	21.970692548170355
58-59	21.827484615908393	28.019542799997527	28.541444711841024	21.61152787225306
60-61	22.113131123053805	28.285475190864663	27.98740740721488	21.613986278866655
62-63	21.83129277124951	28.60763837648043	27.9239445647058	21.63712428756426
64-65	21.83416404813307	28.50281268055469	27.95361390209588	21.709409369216363
66-67	21.942116749837975	28.48067222469301	27.822856307919068	21.754354717549944
68-69	21.893246741371424	28.27374806442228	27.986744969743505	21.846260224462792
70-71	21.937801710627298	27.954407849025465	28.05626895253952	22.051521487807722
72-73	22.11225877786671	27.697490966124438	28.08732513418395	22.102925121824903
74-75	22.03160667154221	27.77553959611409	28.06305263937289	22.12980109297081
76-77	22.1381709900659	27.717964273020907	27.989986988101712	22.15387774881149
78-79	22.122511817917207	27.736803077793105	28.002197829813113	22.138487274476574
80-81	22.134885314163018	27.69485301269896	27.976298781676356	22.193962891461663
82-83	22.19432646010671	27.68235622026304	28.0334443731384	22.08987294649185
84-85	22.33979046898925	27.64522000037486	27.929666616717753	22.08532291391814
86-87	22.134626155097443	27.651463098926076	28.005527334488228	22.208383411488253
88-89	22.21243333741405	27.64678189202341	27.92921244155163	22.211572329010906
90-91	22.260392064359884	27.70129705277067	27.93381102829688	22.104499854572566
92-93	22.289326508963295	27.661246937965995	27.94119279787396	22.108233755196753
94-95	22.235217759948853	27.77605504760225	27.843702089930595	22.145025102518307
96-97	22.28839204939512	27.85105620395431	27.937166677689223	21.923385068961345
98-99	23.39803766153978	29.72422435832216	28.19829864252585	18.67943933761221
100	22.230360808751637	30.678370539906823	22.134894561107107	24.956374090234434
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	822.0
1	825.5
2	1225.5
3	1877.0
4	2473.5
5	3164.0
6	3774.0
7	4959.5
8	6582.0
9	7703.0
10	8136.0
11	8438.5
12	9341.5
13	11092.5
14	14451.0
15	18085.0
16	20211.0
17	21005.0
18	20664.0
19	19653.0
20	18407.0
21	17938.5
22	19516.5
23	23379.0
24	29646.5
25	38219.5
26	49870.0
27	63563.5
28	77002.0
29	91693.0
30	106385.0
31	121016.5
32	136913.5
33	151793.0
34	166669.0
35	181095.5
36	196482.5
37	210966.0
38	221419.0
39	230171.0
40	239692.5
41	247693.5
42	257048.0
43	279892.5
44	287615.0
45	280732.0
46	276380.0
47	267345.0
48	260904.0
49	253762.0
50	246850.5
51	238968.0
52	227386.5
53	214081.0
54	200123.5
55	184119.5
56	167913.5
57	151636.0
58	133953.5
59	115643.0
60	96572.0
61	78137.0
62	61940.5
63	48102.5
64	36149.5
65	26723.0
66	19641.0
67	14758.0
68	11176.5
69	8071.5
70	5685.5
71	4019.0
72	2855.0
73	1955.0
74	1335.0
75	862.5
76	528.5
77	311.5
78	196.0
79	125.0
80	77.5
81	53.5
82	44.0
83	28.5
84	15.0
85	8.0
86	4.5
87	3.0
88	2.0
89	3.0
90	3.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.4792031422897616E-4
3	0.0
4	0.0
5	0.00904592816995338
6	4.428076726550606E-4
7	0.0
8	1.7396015711448808E-4
9	1.7396015711448808E-4
10-11	0.0
12-13	0.0
14-15	7.907279868840368E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.6606671769923262E-4
26-27	7.908607499052351E-6
28-29	6.407747773960289E-4
30-31	3.087731232472672E-4
32-33	0.001554510209126831
34-35	3.418450362391513E-4
36-37	0.001499764066370942
38-39	7.456872217353873E-4
40-41	7.336582068619455E-4
42-43	1.1350804058528638E-4
44-45	7.346214348821765E-5
46-47	5.34685017056452E-4
48-49	0.0016413547111771116
50-51	0.0017867943539970257
52-53	0.0017402152822847763
54-55	0.0013037993984405035
56-57	0.0031394249324255847
58-59	4.2986547102165097E-4
60-61	2.5991254462698392E-5
62-63	0.0020782127510053138
64-65	0.002517743267479675
66-67	0.003155042054933104
68-69	7.994201060444243E-4
70-71	0.005011990642831382
72-73	0.0011862338390582297
74-75	0.0043235965197460325
76-77	3.18252740039087E-4
78-79	0.0010580911902666192
80-81	0.0
82-83	5.7746311598712414E-5
84-85	9.41995967091905E-4
86-87	0.0
88-89	1.97932927062805E-5
90-91	0.003098357870328726
92-93	6.459626225840241E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	49.0
20-21	150.0
22-23	255.0
24-25	399.0
26-27	1294.0
28-29	3356.0
30-31	10518.0
32-33	13667.0
34-35	19028.0
36-37	29888.0
38-39	34556.0
40-41	34062.0
42-43	39118.0
44-45	46637.0
46-47	47389.0
48-49	44175.0
50-51	41162.0
52-53	40895.0
54-55	44431.0
56-57	45209.0
58-59	44739.0
60-61	45151.0
62-63	45655.0
64-65	50284.0
66-67	57695.0
68-69	61670.0
70-71	70722.0
72-73	50728.0
74-75	46640.0
76-77	48817.0
78-79	49420.0
80-81	48663.0
82-83	46558.0
84-85	46984.0
86-87	48836.0
88-89	49368.0
90-91	49111.0
92-93	49044.0
94-95	58874.0
96-97	190047.0
98-99	419421.0
100-101	4248622.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.89028233919586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.88070771005484	77.1987936206335
2	7.208035634470524	12.382004315113162
3	1.7067953052133318	4.3979139197996115
4	0.5765374284740749	1.9807585004300892
5	0.27313630147906953	1.1729877025560502
6	0.12263045515770293	0.6319658650127522
7	0.0723288946199918	0.43486444261351287
8	0.043007103172948896	0.2955113787292406
9	0.02724600798307063	0.21061505864537286
>10	0.08776573390883524	1.108035937952389
>50	0.0013506694647430937	0.0802460452245133
>100	4.0315884002947104E-4	0.06588067299220528
>500	2.7798581730876473E-5	0.015853850142401437
>1k	2.779857911785549E-5	0.024568690155238538
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.162911947536147E-5	0.0	0.0	0.0
3	0.0	3.162911947536147E-5	0.0	0.0	0.0
4	0.0	4.744367921304221E-5	0.0	0.0	0.0
5	0.0	4.744367921304221E-5	0.0	0.0	0.0
6	0.0	4.744367921304221E-5	0.0	0.0	0.0
7	0.0	4.744367921304221E-5	0.0	0.0	0.0
8	0.0	4.744367921304221E-5	0.0	0.0	0.0
9	0.0	1.1070191816376515E-4	0.0	0.0	1.5814559737680733E-5
10-11	0.0	1.1070191816376515E-4	0.0	0.0	1.5814559737680733E-5
12-13	0.0	1.1070191816376515E-4	0.0	0.0	1.5814559737680733E-5
14-15	0.0	1.660528772456477E-4	0.0	0.0	2.37218396065211E-5
16-17	0.0	1.7396015711448808E-4	0.0	0.0	3.162911947536147E-5
18-19	0.0	1.8186743698332845E-4	0.0	0.0	4.744367921304221E-5
20-21	0.0	1.8977471685216883E-4	0.0	0.0	4.744367921304221E-5
22-23	0.0	2.5303295580289173E-4	0.0	0.0	8.698007855724404E-5
24-25	0.0	2.8466207527825323E-4	0.0	0.0	1.3442375777028624E-4
26-27	0.0	3.00476635015934E-4	0.0	7.116551881956331E-5	1.7396015711448808E-4
28-29	0.0	3.00476635015934E-4	0.0	2.7675479540941285E-4	1.7396015711448808E-4
30-31	0.0	3.321057544912954E-4	0.0	9.251517446543229E-4	1.7396015711448808E-4
32-33	0.0	3.321057544912954E-4	0.0	0.0023168330015702274	1.7396015711448808E-4
34-35	0.0	3.87456713573178E-4	0.0	0.00408806369219047	1.7396015711448808E-4
36-37	0.0	4.428076726550606E-4	0.0	0.007156088281300533	1.8977471685216883E-4
38-39	0.0	4.428076726550606E-4	0.0	0.011441833970212012	2.0558927658984955E-4
40-41	0.0	4.902513518681028E-4	0.0	0.01526105014686191	2.0558927658984955E-4
42-43	0.0	5.060659116057835E-4	0.0	0.019459815757216142	2.0558927658984955E-4
44-45	0.0	5.456023109499853E-4	0.0	0.024196276398651524	2.214038363275303E-4
46-47	0.0	5.851387102941872E-4	0.0	0.02939135927247965	2.688475155405725E-4
48-49	0.0	5.930459901630276E-4	0.0	0.03479993870276646	3.00476635015934E-4
50-51	1.5814559737680733E-5	6.800260687202716E-4	0.0	0.04039829284990544	3.00476635015934E-4
52-53	1.5814559737680733E-5	6.800260687202716E-4	0.0	0.045031958853045895	3.3210575449129547E-4
54-55	1.5814559737680733E-5	6.800260687202716E-4	0.0	0.05011633980871025	3.716421538354973E-4
56-57	1.5814559737680733E-5	6.958406284579523E-4	0.0	0.055406310040964454	3.87456713573178E-4
58-59	1.5814559737680733E-5	7.116551881956331E-4	0.0	0.060791167631644744	3.9536399344201835E-4
60-61	1.5814559737680733E-5	7.432843076709946E-4	0.0	0.06589927042691562	3.9536399344201835E-4
62-63	1.5814559737680733E-5	7.828207070151963E-4	0.0	0.07073852570664593	4.111785531796991E-4
64-65	1.5814559737680733E-5	9.172444647854826E-4	0.0	0.07522986067214726	4.111785531796991E-4
66-67	1.5814559737680733E-5	9.172444647854826E-4	0.0	0.0798635266752877	4.111785531796991E-4
68-69	1.5814559737680733E-5	9.251517446543229E-4	0.0	0.08467906011541149	4.1908583304853947E-4
70-71	1.5814559737680733E-5	9.409663043920037E-4	0.0	0.08905969316274906	4.428076726550606E-4
72-73	1.5814559737680733E-5	9.488735842608441E-4	0.0	0.09270494918228447	4.586222323927413E-4
74-75	1.5814559737680733E-5	9.646881439985249E-4	0.0	0.09539342433769019	4.586222323927413E-4
76-77	1.5814559737680733E-5	0.0010200391030804074	0.0	0.0981372504521778	4.586222323927413E-4
78-79	1.5814559737680733E-5	0.0010437609426869284	0.0	0.09975824282529008	4.586222323927413E-4
80-81	1.5814559737680733E-5	0.0010437609426869284	0.0	0.10032756697584658	4.744367921304221E-4
82-83	1.5814559737680733E-5	0.0010437609426869284	0.0	0.10084944744719004	5.218804713434642E-4
84-85	1.5814559737680733E-5	0.0010674827822934494	0.0	0.10107085128351757	5.218804713434642E-4
86-87	1.5814559737680733E-5	0.0011070191816376514	0.0	0.10114992408220598	5.297877512123046E-4
88	1.5814559737680733E-5	0.0011544628608506937	0.0	0.10114992408220598	5.37695031081145E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7250	0.0	49.595993	1
CTTATAC	6255	0.0	41.894947	1
GTATCAA	13050	0.0	37.330315	1
TATACAC	8605	0.0	33.898308	3
ATCAACG	15530	0.0	31.147707	3
TCAACGC	15565	0.0	30.967266	4
TTATACA	8940	0.0	30.802052	2
CAACGCA	15970	0.0	30.208834	5
TATCAAC	16550	0.0	29.409729	2
AACGCAG	16675	0.0	28.928894	6
ACGCAGA	18940	0.0	25.491615	7
CGCAGAG	19315	0.0	25.018936	8
ACACATC	11050	0.0	24.490417	6
ACATCTC	11370	0.0	23.800776	8
TACACAT	12045	0.0	23.575113	5
CACATCT	12520	0.0	21.511677	7
GCAGAGT	23055	0.0	20.86719	9
ATACACA	15655	0.0	20.059654	4
GTGGTAT	3735	0.0	20.013256	1
CATCTCC	13440	0.0	19.87935	9
>>END_MODULE