FastQCFastQC Report
Fri 27 May 2016
SRR2140050_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140050_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6250578
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80400.050.9986721
GTATCAA147350.036.8821331
CTTATAC55600.036.3398481
TCAACGC176200.030.4586224
ATCAACG176000.030.4210323
CAACGCA178550.030.0340165
TATCAAC184250.029.2658022
AACGCAG187050.028.7570156
TATACAC83900.027.4648043
ACGCAGA212900.025.265187
TGGTATC35250.025.1146262
CGCAGAG215900.024.8944978
TTATACA88400.024.389622
GTGGTAT37400.024.3504161
ACATCTC92850.022.5340588
ACACATC97150.021.3624536
GCAGAGT257100.020.8228119
TACACAT112700.020.521465
TATGCCG57450.020.40349242-43
CTCGTAT57350.019.8843438-39
Produced by FastQC (version 0.11.5)