##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140050_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6250578
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43418912618961	33.0	32.0	33.0	27.0	34.0
2	31.95109380284511	33.0	32.0	33.0	30.0	34.0
3	32.674989896934335	33.0	33.0	34.0	31.0	34.0
4	32.66464525360695	33.0	33.0	34.0	31.0	34.0
5	33.19980424210369	33.0	33.0	34.0	33.0	34.0
6	37.28751933021234	38.0	38.0	38.0	36.0	38.0
7	37.385658414309844	38.0	38.0	38.0	37.0	38.0
8	37.51834870311193	38.0	38.0	38.0	37.0	38.0
9	37.534548005000495	38.0	38.0	38.0	37.0	38.0
10-11	37.55090721210102	38.0	38.0	38.0	37.5	38.0
12-13	37.570804731978384	38.0	38.0	38.0	38.0	38.0
14-15	37.54218177902908	38.0	38.0	38.0	38.0	38.0
16-17	37.56951277145889	38.0	38.0	38.0	38.0	38.0
18-19	37.4851301751614	38.0	38.0	38.0	37.5	38.0
20-21	37.56220529030713	38.0	38.0	38.0	38.0	38.0
22-23	37.570071794408534	38.0	38.0	38.0	38.0	38.0
24-25	37.571517708163526	38.0	38.0	38.0	38.0	38.0
26-27	37.546260942458886	38.0	38.0	38.0	38.0	38.0
28-29	37.479288694397894	38.0	38.0	38.0	37.0	38.0
30-31	37.51599325580865	38.0	38.0	38.0	37.5	38.0
32-33	37.50585682122609	38.0	38.0	38.0	38.0	38.0
34-35	37.48474210763841	38.0	38.0	38.0	37.0	38.0
36-37	37.46245932744466	38.0	38.0	38.0	37.0	38.0
38-39	37.4434846137116	38.0	38.0	38.0	37.0	38.0
40-41	37.432922314830776	38.0	38.0	38.0	37.0	38.0
42-43	37.36845460432083	38.0	38.0	38.0	37.0	38.0
44-45	37.377641187189965	38.0	38.0	38.0	37.0	38.0
46-47	37.35613573350566	38.0	38.0	38.0	37.0	38.0
48-49	37.32405059568407	38.0	38.0	38.0	37.0	38.0
50-51	37.29344475715769	38.0	38.0	38.0	37.0	38.0
52-53	37.27780951879437	38.0	38.0	38.0	37.0	38.0
54-55	37.26687697576704	38.0	38.0	38.0	37.0	38.0
56-57	37.27236124859638	38.0	38.0	38.0	37.0	38.0
58-59	37.26984919445751	38.0	38.0	38.0	37.0	38.0
60-61	37.271208401015215	38.0	38.0	38.0	37.0	38.0
62-63	37.277750232748474	38.0	38.0	38.0	37.0	38.0
64-65	37.28183849401736	38.0	38.0	38.0	37.0	38.0
66-67	37.28584529190228	38.0	38.0	38.0	37.0	38.0
68-69	37.2860060428263	38.0	38.0	38.0	37.0	38.0
70-71	37.29302585937978	38.0	38.0	38.0	37.0	38.0
72-73	37.26847518779232	38.0	38.0	38.0	37.0	38.0
74-75	37.27054349074277	38.0	38.0	38.0	37.0	38.0
76-77	37.270959237004	38.0	38.0	38.0	37.0	38.0
78-79	37.25987828443134	38.0	38.0	38.0	37.0	38.0
80-81	37.255239678742115	38.0	38.0	38.0	37.0	38.0
82-83	37.260720837056404	38.0	38.0	38.0	37.0	38.0
84-85	37.24979015961927	38.0	38.0	38.0	37.0	38.0
86-87	37.23782006905108	38.0	38.0	38.0	37.0	38.0
88-89	37.222620431556436	38.0	38.0	38.0	37.0	38.0
90-91	37.21429633075605	38.0	38.0	38.0	36.5	38.0
92-93	37.21438132605774	38.0	38.0	38.0	37.0	38.0
94-95	37.17997550626232	38.0	38.0	38.0	36.0	38.0
96-97	37.19529470841421	38.0	38.0	38.0	36.0	38.0
98-99	37.18896333318123	38.0	38.0	38.0	36.0	38.0
100	35.72767306961011	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	18.0
20	58.0
21	159.0
22	416.0
23	926.0
24	2023.0
25	3897.0
26	6732.0
27	10909.0
28	17189.0
29	25723.0
30	36448.0
31	48424.0
32	62977.0
33	82370.0
34	117214.0
35	210051.0
36	641915.0
37	4983127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.76311022756616	20.997658136575527	14.420234416721142	23.818997219137174
2	14.27945656069216	20.950586827420263	42.45458006962576	22.315376542261813
3	17.612131230103838	26.59720429054721	30.916820812411267	24.87384366693768
4	12.080786768839618	18.60746318180495	39.428913614068975	29.882836435286464
5	12.939440765715965	37.8902811275309	36.015350927017174	13.154927179735957
6	29.12854068841942	38.39398132964299	19.166857316556143	13.310620665381446
7	25.775200309475384	33.09724316695192	23.369022832768426	17.758533690804274
8	23.843370594965968	36.87346574139727	21.724717850002946	17.558445813633817
9	25.258552833666105	17.017339246403523	21.58731377668561	36.136794143244764
10-11	23.63204650833891	27.079519686019438	29.42650263703613	19.861931168605526
12-13	24.601180882791958	24.63588167366282	29.387834213091974	21.37510323045325
14-15	21.802041491366197	26.113291131899413	27.035605910013917	25.049061466720474
16-17	21.285199544746103	29.239711911442427	27.778127078807753	21.696961465003717
18-19	21.343282173264615	28.201327941192	29.56652168807429	20.888868197469098
20-21	22.20911839631569	27.283098795025744	29.40242287545634	21.10535993320222
22-23	21.86047181357844	27.45710240226547	29.238930619885284	21.4434951642708
24-25	21.736377743975172	27.529599547904798	29.366612314762325	21.36741039335771
26-27	21.746358912010795	27.636745518036264	29.285798064689665	21.331097505263273
28-29	21.521659966497822	27.57312494647735	29.39103828740323	21.514176799621602
30-31	21.77271001909756	27.49755612422211	29.42077940930709	21.30895444737323
32-33	21.383557814146684	27.443937604948133	29.531788214207317	21.64071636669787
34-35	21.512110845892735	27.5290688966912	29.3999991625813	21.55882109483477
36-37	21.52009868201864	27.514316461731443	29.540616571773477	21.42496828447644
38-39	21.432731849618357	27.53191259129143	29.532263254520892	21.50309230456932
40-41	21.66619030030736	27.409538847335362	29.467072983967064	21.457197868390214
42-43	21.777924579437844	27.41523475270219	29.260293607534354	21.546547060325615
44-45	21.657259456141762	27.67007456389134	29.136216253239272	21.53644972672763
46-47	21.856370366654087	27.464634056511343	28.81483966046278	21.864155916371786
48-49	21.73450311191604	27.564014103311514	28.883257558145342	21.818225226627103
50-51	21.64432801001903	27.689679201640327	28.869581062345446	21.7964117259952
52-53	21.812341099770794	27.637659872977956	28.953263813075225	21.59673521417603
54-55	21.778699818220442	27.620283435124037	28.727134203908587	21.87388254274693
56-57	21.9603012122763	27.832340923504006	28.456924414052732	21.750433450166963
58-59	21.63655032598221	28.03542501825601	28.81897242371843	21.50905223204335
60-61	21.89280896171425	28.26512410186417	28.412880456551875	21.429186479869703
62-63	21.697750897471792	28.46253652401997	28.317165319134546	21.52254725937369
64-65	21.626772222761616	28.440918514025597	28.346200573307634	21.586108689905153
66-67	21.732584592417496	28.420979215428293	28.22262309203068	21.623813100123527
68-69	21.743733004857415	28.250642619750387	28.30155018941595	21.704074185976246
70-71	21.776357100155735	27.969703568903782	28.421534868035174	21.832404462905313
72-73	21.877786367306243	27.77746427587574	28.431074435625085	21.91367492119293
74-75	21.85434195254218	27.846366495374138	28.343561118683088	21.955730433400596
76-77	21.90761050247473	27.836790116343803	28.27954161648044	21.976057764701025
78-79	21.896723320456758	27.826796266737187	28.29697628694932	21.97950412585673
80-81	21.933122997765274	27.861568055041896	28.20686308817681	21.998445859016016
82-83	21.99412030004872	27.82397302598401	28.283166832338047	21.898739841629222
84-85	22.107079489441066	27.78414467293112	28.172784424653713	21.935991412974097
86-87	21.917270901767594	27.807132552836027	28.2476904688963	22.027906076500077
88-89	22.01691706122632	27.777588457483347	28.180141123041523	22.025353358248807
90-91	22.06666146044256	27.829888484151095	28.180089387995043	21.923360667411306
92-93	22.097380865231823	27.805393057321343	28.160001666945377	21.937224410501457
94-95	22.019829186636738	27.86458338936105	28.129945006418893	21.985642417583314
96-97	22.055590404176936	27.99737148076312	28.22938456168162	21.71765355337832
98-99	23.20195522154287	29.833783489760034	28.430691620286737	18.533569668410358
100	22.022462067139323	30.76488893401743	22.390740818167156	24.821908180676093
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1113.0
1	1054.0
2	1494.0
3	2261.5
4	2840.5
5	3583.0
6	4355.5
7	5676.0
8	7493.5
9	8891.0
10	9433.5
11	9641.5
12	10646.0
13	12655.5
14	16210.0
15	20047.5
16	22437.0
17	23568.5
18	23226.0
19	21732.5
20	20567.5
21	20489.0
22	21923.5
23	26340.5
24	33672.5
25	43396.0
26	56270.5
27	70693.0
28	85625.5
29	101843.5
30	117202.5
31	131812.0
32	147588.5
33	163136.0
34	178246.5
35	192468.5
36	207038.0
37	219714.5
38	228825.5
39	235947.5
40	243474.5
41	251098.0
42	254840.5
43	257726.5
44	269989.0
45	282953.0
46	276927.5
47	268011.5
48	261732.0
49	251488.5
50	243508.0
51	233739.5
52	221648.0
53	209035.0
54	195523.5
55	180356.5
56	164343.5
57	147028.0
58	129047.5
59	111365.5
60	93286.0
61	75319.5
62	58587.0
63	44893.5
64	33890.0
65	25028.5
66	18117.5
67	13373.0
68	9956.0
69	7126.5
70	5094.0
71	3681.5
72	2624.5
73	1826.0
74	1210.0
75	753.5
76	496.5
77	315.0
78	199.5
79	124.5
80	83.5
81	61.5
82	40.5
83	29.5
84	23.0
85	14.0
86	8.5
87	6.5
88	4.5
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.8797336822290677E-4
3	0.0
4	0.0
5	0.008511212883032577
6	4.4795857279118827E-4
7	0.0
8	3.1997040913656305E-5
9	2.8797336822290677E-4
10-11	0.0
12-13	0.0
14-15	7.999260228414076E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.0399970880081536E-4
26-27	4.8004301185386205E-5
28-29	6.96289823676207E-4
30-31	3.3647742108322657E-4
32-33	0.0016298459313866978
34-35	3.462392260216654E-4
36-37	0.001520251857214064
38-39	7.322380408238979E-4
40-41	7.780135022195906E-4
42-43	1.3192343460683147E-4
44-45	6.649767391136658E-5
46-47	5.956581221047135E-4
48-49	0.001693208557408321
50-51	0.0018101827046435029
52-53	0.0018938112486534572
54-55	0.0013282345332297368
56-57	0.003258818806082673
58-59	4.4208574995824497E-4
60-61	4.461510017696133E-5
62-63	0.0020980413838212735
64-65	0.002499931138260464
66-67	0.0032620275550359176
68-69	7.530098780951378E-4
70-71	0.0047879727629255514
72-73	0.0012486963752822555
74-75	0.004151303552658612
76-77	3.6982601243277195E-4
78-79	0.001141248376180651
80-81	0.0
82-83	3.0167768996945814E-5
84-85	9.552792133783805E-4
86-87	0.0
88-89	1.0389527273392201E-5
90-91	0.0032476534390127472
92-93	5.634413723305637E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	44.0
20-21	136.0
22-23	296.0
24-25	462.0
26-27	1421.0
28-29	4142.0
30-31	12695.0
32-33	16779.0
34-35	23090.0
36-37	35769.0
38-39	40523.0
40-41	40241.0
42-43	46469.0
44-45	54813.0
46-47	54791.0
48-49	51060.0
50-51	47702.0
52-53	47981.0
54-55	51496.0
56-57	52620.0
58-59	51750.0
60-61	50945.0
62-63	51732.0
64-65	56339.0
66-67	61874.0
68-69	64044.0
70-71	71085.0
72-73	56045.0
74-75	53064.0
76-77	54937.0
78-79	55710.0
80-81	55083.0
82-83	52358.0
84-85	52794.0
86-87	54034.0
88-89	55187.0
90-91	54457.0
92-93	53911.0
94-95	63171.0
96-97	185148.0
98-99	396228.0
100-101	4018152.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.72301202928851
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.88457931624495	79.72669044635781
2	6.715112529885103	11.781397944742736
3	1.4932823247167846	3.929856700027632
4	0.46986140763555306	1.648706316564482
5	0.1974320195747895	0.8659665714062992
6	0.08709150893550803	0.45839576915990743
7	0.046923523413124765	0.2881390966178301
8	0.029263235470924936	0.205364732578548
9	0.02024390083999362	0.15982703612158353
>10	0.05447492310339206	0.7160995619631443
>50	9.601304429627348E-4	0.05643995400515052
>100	6.720081934170984E-4	0.09865356239738057
>500	1.0317154352557325E-4	0.06446230805744387
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5998520456828152E-5	0.0	0.0	0.0
3	0.0	1.5998520456828152E-5	0.0	0.0	0.0
4	0.0	4.799556137048446E-5	0.0	0.0	0.0
5	0.0	1.1198964319779707E-4	0.0	3.1997040913656305E-5	0.0
6	0.0	1.1198964319779707E-4	0.0	3.1997040913656305E-5	0.0
7	0.0	1.1198964319779707E-4	0.0	6.399408182731261E-5	0.0
8	0.0	1.2798816365462522E-4	0.0	6.399408182731261E-5	0.0
9	0.0	2.07980765938766E-4	0.0	7.999260228414076E-5	0.0
10-11	0.0	2.2397928639559413E-4	0.0	7.999260228414076E-5	0.0
12-13	0.0	2.4797706708083636E-4	0.0	9.599112274096891E-5	0.0
14-15	0.0	3.439681898218053E-4	0.0	9.599112274096891E-5	1.5998520456828152E-5
16-17	0.0	3.599667102786334E-4	0.0	9.599112274096891E-5	1.5998520456828152E-5
18-19	0.0	3.759652307354616E-4	0.0	1.1198964319779707E-4	1.5998520456828152E-5
20-21	0.0	4.15961531877532E-4	0.0	1.2798816365462522E-4	1.5998520456828152E-5
22-23	0.0	4.239607921059461E-4	0.0	1.2798816365462522E-4	4.799556137048446E-5
24-25	0.0	4.8795487393325863E-4	0.0	1.5198594433986747E-4	7.999260228414076E-5
26-27	0.0	5.359504353037431E-4	0.0	1.9198224548193783E-4	7.999260228414076E-5
28-29	0.0	5.599482159889854E-4	0.0	4.639570932480164E-4	7.999260228414076E-5
30-31	0.0	5.999445171310557E-4	0.0	0.0013998705399724633	7.999260228414076E-5
32-33	0.0	6.079437773594698E-4	0.0	0.002919729983371138	7.999260228414076E-5
34-35	0.0	6.719378591867823E-4	0.0	0.004863550218875759	7.999260228414076E-5
36-37	0.0	7.759282421561654E-4	0.0	0.00787127206475945	7.999260228414076E-5
38-39	0.0	9.119156660392047E-4	0.0	0.012022888123306357	9.599112274096891E-5
40-41	0.0	9.43912706952861E-4	0.0	0.016822444260354803	9.599112274096891E-5
42-43	0.0	9.919082683233454E-4	0.0	0.021622000397403248	9.599112274096891E-5
44-45	0.0	0.0011038979115211424	0.0	0.0272214825572931	9.599112274096891E-5
46-47	0.0	0.0012158875547189395	0.0	0.033132935866091104	9.599112274096891E-5
48-49	0.0	0.0012398853354041819	0.0	0.038700420985067305	1.1198964319779707E-4
50-51	0.0	0.0013358764581451509	0.0	0.04487584988140297	1.1198964319779707E-4
52-53	0.0	0.0013518749786019788	0.0	0.05081130097088621	1.1998890342621114E-4
54-55	0.0	0.0013918712797440494	0.0	0.056834743922882014	1.2798816365462522E-4
56-57	0.0	0.0014558653615713618	0.0	0.06285018761464939	1.2798816365462522E-4
58-59	0.0	0.0015038609229418463	0.0	0.06882563500527471	1.359874238830393E-4
60-61	7.999260228414076E-6	0.0015198594433986745	0.0	0.07420113787876897	1.5198594433986747E-4
62-63	1.5998520456828152E-5	0.001567855004769159	0.0	0.07951264667043592	1.5998520456828152E-4
64-65	1.5998520456828152E-5	0.0017438387297942686	0.0	0.08488814954393017	1.5998520456828152E-4
66-67	1.5998520456828152E-5	0.0018158320718499954	0.0	0.09039963984130747	1.5998520456828152E-4
68-69	1.5998520456828152E-5	0.0018558283729920657	0.0	0.09613510942508037	1.5998520456828152E-4
70-71	1.5998520456828152E-5	0.001895824674134136	0.0	0.10104665520532662	1.5998520456828152E-4
72-73	1.5998520456828152E-5	0.0019198224548193784	0.0	0.10519827126387352	1.9198224548193783E-4
74-75	1.5998520456828152E-5	0.0019438202355046208	0.0	0.10827798645181294	1.999815057103519E-4
76-77	1.5998520456828152E-5	0.0020398113582455893	0.0	0.11120571569541249	2.07980765938766E-4
78-79	1.5998520456828152E-5	0.002111804700301316	0.0	0.113205530752516	2.07980765938766E-4
80-81	1.5998520456828152E-5	0.002111804700301316	0.0	0.1139414626935301	2.1598002616718008E-4
82-83	1.5998520456828152E-5	0.002175798782128629	0.0	0.11463739833340213	2.2397928639559413E-4
84-85	1.5998520456828152E-5	0.0022797891650980116	0.0	0.1149573687425387	2.3197854662400822E-4
86-87	1.5998520456828152E-5	0.0024797706708083638	0.0	0.11509335616642173	2.399778068524223E-4
88	1.5998520456828152E-5	0.002527766232178848	0.0	0.11512535320733538	2.399778068524223E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8040	0.0	50.998672	1
GTATCAA	14735	0.0	36.882133	1
CTTATAC	5560	0.0	36.339848	1
TCAACGC	17620	0.0	30.458622	4
ATCAACG	17600	0.0	30.421032	3
CAACGCA	17855	0.0	30.034016	5
TATCAAC	18425	0.0	29.265802	2
AACGCAG	18705	0.0	28.757015	6
TATACAC	8390	0.0	27.464804	3
ACGCAGA	21290	0.0	25.26518	7
TGGTATC	3525	0.0	25.114626	2
CGCAGAG	21590	0.0	24.894497	8
TTATACA	8840	0.0	24.38962	2
GTGGTAT	3740	0.0	24.350416	1
ACATCTC	9285	0.0	22.534058	8
ACACATC	9715	0.0	21.362453	6
GCAGAGT	25710	0.0	20.822811	9
TACACAT	11270	0.0	20.52146	5
TATGCCG	5745	0.0	20.403492	42-43
CTCGTAT	5735	0.0	19.88434	38-39
>>END_MODULE