FastQCFastQC Report
Fri 27 May 2016
SRR2140049_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140049_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5451023
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69100.048.9824751
GTATCAA123900.035.8699041
ATCAACG143300.030.7743323
TCAACGC144450.030.5293314
CAACGCA147600.029.8777925
TATCAAC152650.029.1703932
AACGCAG152850.028.933646
CTTATAC41750.027.7427221
ACGCAGA176650.024.9625747
CGCAGAG177500.024.8430358
TATACAC62850.022.3877433
GCAGAGT214300.020.5769429
GAGTACT130500.019.8370312-13
GTGGTAT33200.019.7694741
TTATACA69800.018.9294092
TGGTATC33100.018.9219822
CAGAGTA205950.018.70356610-11
AGAGTAC190150.018.35148210-11
GTACATG144650.018.2092321
TATGCCG36050.018.16910242-43
Produced by FastQC (version 0.11.5)