FastQCFastQC Report
Fri 27 May 2016
SRR2140048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6383959
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75800.041.110121
CTTATAC61850.037.960241
GTATCAA127000.034.8446661
TCAACGC143500.030.210244
ATCAACG145000.029.868123
TATACAC89500.029.7340013
CAACGCA148100.029.3008925
AACGCAG154250.028.35266
TATCAAC158500.027.6218152
TTATACA92250.027.0328032
ACGCAGA173850.024.98317
CGCAGAG174850.024.9138558
ACATCTC107700.022.4353688
ACACATC108450.022.3595376
TACACAT123650.022.2856675
GCAGAGT214000.020.2958499
CACATCT122100.019.7894297
GTACATG148150.019.7589591
TACATGG148700.019.5126882
CATCTCC126950.018.9657789
Produced by FastQC (version 0.11.5)