##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140048_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6383959
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52843650781592	33.0	32.0	33.0	27.0	34.0
2	31.990611625168647	33.0	32.0	33.0	30.0	34.0
3	32.687085709666995	33.0	33.0	34.0	31.0	34.0
4	32.68547730334734	33.0	33.0	34.0	31.0	34.0
5	33.20726903164635	34.0	33.0	34.0	33.0	34.0
6	37.27140164903941	38.0	38.0	38.0	36.0	38.0
7	37.368890527022494	38.0	38.0	38.0	37.0	38.0
8	37.49034697747902	38.0	38.0	38.0	37.0	38.0
9	37.50926846491339	38.0	38.0	38.0	37.0	38.0
10-11	37.527530878566104	38.0	38.0	38.0	37.0	38.0
12-13	37.55085887926285	38.0	38.0	38.0	37.5	38.0
14-15	37.520744494129744	38.0	38.0	38.0	37.0	38.0
16-17	37.54520118315296	38.0	38.0	38.0	38.0	38.0
18-19	37.4611690801899	38.0	38.0	38.0	37.0	38.0
20-21	37.538421929541144	38.0	38.0	38.0	37.5	38.0
22-23	37.54762102250054	38.0	38.0	38.0	38.0	38.0
24-25	37.54986519587425	38.0	38.0	38.0	38.0	38.0
26-27	37.52795170986515	38.0	38.0	38.0	38.0	38.0
28-29	37.45849794574498	38.0	38.0	38.0	37.0	38.0
30-31	37.49655773405718	38.0	38.0	38.0	37.0	38.0
32-33	37.485436133782834	38.0	38.0	38.0	37.0	38.0
34-35	37.462747285391835	38.0	38.0	38.0	37.0	38.0
36-37	37.44405498165631	38.0	38.0	38.0	37.0	38.0
38-39	37.422265192254	38.0	38.0	38.0	37.0	38.0
40-41	37.423487942371025	38.0	38.0	38.0	37.0	38.0
42-43	37.3666698730046	38.0	38.0	38.0	37.0	38.0
44-45	37.38026567466478	38.0	38.0	38.0	37.0	38.0
46-47	37.36779586053815	38.0	38.0	38.0	37.0	38.0
48-49	37.33976060408744	38.0	38.0	38.0	37.0	38.0
50-51	37.31233321753925	38.0	38.0	38.0	37.0	38.0
52-53	37.29562872106479	38.0	38.0	38.0	37.0	38.0
54-55	37.28450183909007	38.0	38.0	38.0	37.0	38.0
56-57	37.28853538785606	38.0	38.0	38.0	37.0	38.0
58-59	37.28330277240555	38.0	38.0	38.0	37.0	38.0
60-61	37.28172262039175	38.0	38.0	38.0	37.0	38.0
62-63	37.28059610638571	38.0	38.0	38.0	37.0	38.0
64-65	37.279957294068026	38.0	38.0	38.0	37.0	38.0
66-67	37.27802067978465	38.0	38.0	38.0	37.0	38.0
68-69	37.27755476037909	38.0	38.0	38.0	37.0	38.0
70-71	37.27981953318751	38.0	38.0	38.0	37.0	38.0
72-73	37.253335249753505	38.0	38.0	38.0	37.0	38.0
74-75	37.25112897219981	38.0	38.0	38.0	37.0	38.0
76-77	37.25123059967858	38.0	38.0	38.0	37.0	38.0
78-79	37.236809741521824	38.0	38.0	38.0	37.0	38.0
80-81	37.22968341000721	38.0	38.0	38.0	37.0	38.0
82-83	37.23318637519962	38.0	38.0	38.0	37.0	38.0
84-85	37.21769029629783	38.0	38.0	38.0	37.0	38.0
86-87	37.20251923001854	38.0	38.0	38.0	36.5	38.0
88-89	37.1858541011313	38.0	38.0	38.0	36.5	38.0
90-91	37.175439179596594	38.0	38.0	38.0	36.0	38.0
92-93	37.172713426443885	38.0	38.0	38.0	36.0	38.0
94-95	37.13396009015301	38.0	38.0	38.0	36.0	38.0
96-97	37.14590342828372	38.0	38.0	38.0	36.0	38.0
98-99	37.133380727760766	38.0	38.0	38.0	36.0	38.0
100	35.64869145025746	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	19.0
20	79.0
21	175.0
22	415.0
23	1036.0
24	2201.0
25	4090.0
26	7167.0
27	11890.0
28	18044.0
29	26701.0
30	37392.0
31	49900.0
32	64890.0
33	85605.0
34	124399.0
35	219412.0
36	648389.0
37	5082150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.46763144938744	20.98439228698054	14.393074892868201	24.154901370763817
2	14.192225207665674	20.895343768165706	42.44326269408276	22.46916833008586
3	17.673124153836202	26.29108363634541	30.782042929786986	25.2537492800314
4	12.176080704778961	18.497769174269447	39.28366394583674	30.042486175114846
5	13.136255892059676	37.607589961366855	36.07145995874599	13.18469418782748
6	29.330126737097746	38.3077712078026	19.06903966901862	13.293062386081036
7	25.86514105118783	32.80500391684846	23.335221921068104	17.994633110895606
8	23.720577604322084	37.11644270675467	21.712778363882578	17.450201325040666
9	24.94261769813831	17.263061963174138	21.588464923761	36.20585541492656
10-11	23.645609252816318	26.988276397138517	29.430922097087404	19.935192252957766
12-13	24.521578224421553	24.462962559753283	29.357762166079077	21.65769704974609
14-15	21.770160316674993	25.97108048243108	26.789013431086683	25.469745769807243
16-17	21.486917444175315	29.217567030114072	27.50652847237897	21.788987053331642
18-19	21.547060374291252	28.204058014783616	29.103037785800318	21.145843825124818
20-21	22.21674225574218	27.377398216345316	28.984686221923134	21.42117330598937
22-23	21.86990568958669	27.58212048946579	28.820854703345034	21.727119117602484
24-25	21.80367833569327	27.637852209388114	28.951593883064614	21.606875571854
26-27	21.8198452572914	27.762872090348882	28.9175436694427	21.49973898291701
28-29	21.490097303860765	27.617917989813094	29.07355014677307	21.81843455955307
30-31	21.981865280910753	27.376082347327973	29.175202949436663	21.46684942232461
32-33	21.645134999710102	27.759022253186384	28.92211094083965	21.673731806263863
34-35	21.659256617855057	27.801456788085627	29.17613004556912	21.363156548490196
36-37	21.9237331456758	27.565250984048163	28.899568444576378	21.611447425699662
38-39	21.60993019524534	27.519355366345277	29.11595711223074	21.754757326178645
40-41	21.86704146538331	27.37696764533143	29.10694736734471	21.649043521940552
42-43	21.934742827245188	27.391135203302774	28.989140837764648	21.68498113168739
44-45	21.788968022974796	27.62493630537953	28.85882618752295	21.72726948412272
46-47	22.072204541350207	27.394604043359738	28.496827838221584	22.036363577068467
48-49	21.903834870645348	27.44776298322255	28.633491028902586	22.01491111722952
50-51	21.752951756702647	27.636858237383	28.701574661993668	21.908615343920687
52-53	21.956045923154953	27.58665260388662	28.76059917043376	21.696702302524667
54-55	21.863221278301666	27.643411235318254	28.508972057757997	21.98439542862208
56-57	22.05116760077887	27.879823040953166	28.226714142713753	21.842295215554202
58-59	21.73342335480767	28.168526067211996	28.551211141035832	21.546839436944502
60-61	21.96009044391691	28.408629619230837	28.10594922699542	21.52533070985683
62-63	21.8148616366526	28.596054178167936	28.056096691214943	21.532987493964516
64-65	21.778273016358295	28.51970819362845	28.06224894769332	21.639769842319932
66-67	21.832619868645736	28.532895209121634	27.929985834309544	21.704499087923086
68-69	21.84264726736107	28.349807554102735	28.039450672692762	21.768094505843433
70-71	21.887362248983955	28.033080001346033	28.13196671610852	21.94759103356149
72-73	22.024477395995273	27.77813603506009	28.182874408799368	22.014512160145266
74-75	21.918037545759603	27.873858873424407	28.148248254495435	22.05985532632056
76-77	22.038103188624095	27.8578624048111	28.06883359508992	22.035200811474883
78-79	22.04200369591049	27.84730307104691	28.03875747080708	22.07193576223552
80-81	22.051397992468626	27.806653812173938	28.050738703415902	22.091209491941537
82-83	22.078540961399185	27.82831489919407	28.101447066208557	21.991697073198196
84-85	22.20946013470295	27.72815232958248	28.035064211320066	22.0273233243945
86-87	22.026386283940784	27.79049705824899	28.06573309403046	22.117383563779764
88-89	22.119822979999608	27.78140004899921	28.01194863321567	22.08682833778551
90-91	22.144962579583666	27.834082624526204	28.0145059562103	22.00644883967983
92-93	22.16350722295088	27.768942025460248	27.991562745141827	22.075988006447048
94-95	22.108262368949728	27.887213397424947	27.97296033314626	22.03156390047906
96-97	22.20822738281065	27.9474855636499	28.04763319418091	21.79665385935854
98-99	23.30162768677747	29.886946602152175	28.2286154037135	18.582810307356855
100	22.077965065588295	30.796214992696765	22.28027721788629	24.845542723828654
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	772.0
1	744.5
2	1123.0
3	1724.0
4	2210.0
5	2880.0
6	3551.0
7	4528.5
8	5743.0
9	6693.5
10	7209.5
11	7673.5
12	8728.5
13	10447.5
14	13248.5
15	16159.0
16	18039.0
17	19029.5
18	18890.0
19	17889.5
20	17249.5
21	17699.5
22	19577.5
23	23748.5
24	30623.0
25	40221.5
26	52995.5
27	67918.0
28	82346.5
29	97002.5
30	111951.5
31	126686.5
32	142424.0
33	158163.0
34	173543.0
35	188081.0
36	203132.0
37	216947.0
38	227788.0
39	237846.0
40	248564.5
41	257569.5
42	265655.0
43	284949.5
44	294203.0
45	290833.5
46	288159.0
47	278953.0
48	270042.5
49	261180.0
50	252345.5
51	241090.0
52	226648.5
53	211007.0
54	194726.5
55	178031.0
56	161553.0
57	144626.0
58	127210.5
59	109748.0
60	90671.5
61	72698.5
62	57097.5
63	43658.5
64	32607.5
65	23863.5
66	17608.0
67	13453.0
68	10144.5
69	7374.0
70	5262.5
71	3798.0
72	2794.0
73	1880.5
74	1237.0
75	840.5
76	574.0
77	363.5
78	233.5
79	148.0
80	81.0
81	44.5
82	27.0
83	20.5
84	14.5
85	10.5
86	8.0
87	7.5
88	10.0
89	9.0
90	6.5
91	5.0
92	2.5
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.1328522003352466E-4
3	0.0
4	0.0
5	0.009085271380972215
6	5.482491350586681E-4
7	0.0
8	6.265704400670493E-5
9	3.446137420368771E-4
10-11	0.0
12-13	0.0
14-15	7.832130500838116E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.4882574917315548E-4
26-27	3.1334573973565523E-5
28-29	6.033558338044601E-4
30-31	3.0584658688148706E-4
32-33	0.0016183645091091925
34-35	3.701335607265927E-4
36-37	0.0014146825875097754
38-39	7.387868833393549E-4
40-41	9.106797329503579E-4
42-43	1.3658698530107108E-4
44-45	9.707378359875328E-5
46-47	5.299477553043693E-4
48-49	0.0017007465044729631
50-51	0.0017131970715420337
52-53	0.001816705725839772
54-55	0.0015360576953342877
56-57	0.0030370978541930773
58-59	4.3492565585509713E-4
60-61	2.579002364515335E-5
62-63	0.0021934912438950718
64-65	0.002640917661171838
66-67	0.003213953502745767
68-69	8.477917523605647E-4
70-71	0.004997764988671582
72-73	0.0011143402621549405
74-75	0.003945372810537022
76-77	3.44190977247488E-4
78-79	9.861424446133097E-4
80-81	0.0
82-83	0.0
84-85	9.568252422483383E-4
86-87	0.0
88-89	4.9275128369100667E-5
90-91	0.0029671389362806912
92-93	5.029814729792317E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	54.0
20-21	186.0
22-23	322.0
24-25	486.0
26-27	1290.0
28-29	3379.0
30-31	10694.0
32-33	14088.0
34-35	19836.0
36-37	30709.0
38-39	35213.0
40-41	35144.0
42-43	40144.0
44-45	47512.0
46-47	47999.0
48-49	44954.0
50-51	41753.0
52-53	42201.0
54-55	45831.0
56-57	47623.0
58-59	46353.0
60-61	49227.0
62-63	48728.0
64-65	52277.0
66-67	58437.0
68-69	61515.0
70-71	70176.0
72-73	51856.0
74-75	48388.0
76-77	50984.0
78-79	51403.0
80-81	50993.0
82-83	48923.0
84-85	48430.0
86-87	50560.0
88-89	51457.0
90-91	51775.0
92-93	51174.0
94-95	60259.0
96-97	193707.0
98-99	418165.0
100-101	4259754.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.35098754531019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.89860701629671	77.62833494804971
2	7.2967855897840606	12.601692831684847
3	1.6659543659247342	4.315704141090656
4	0.5792190016507809	2.0006453119021432
5	0.24396955177146754	1.0533505863226449
6	0.1203695139683378	0.6236415840908986
7	0.062465598420002835	0.37757762778232085
8	0.04203998523353739	0.29041553930449693
9	0.025192868791572814	0.19578861893265637
>10	0.06464614087537245	0.8095857526315114
>50	4.219367286131634E-4	0.022390558800526764
>100	2.758172771435433E-4	0.046094242415715386
>500	5.261327766363223E-5	0.03477825699187962
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.5664261001676233E-5	0.0
3	0.0	0.0	0.0	1.5664261001676233E-5	0.0
4	0.0	6.265704400670493E-5	0.0	3.1328522003352465E-5	0.0
5	0.0	6.265704400670493E-5	0.0	3.1328522003352465E-5	0.0
6	0.0	6.265704400670493E-5	0.0	3.1328522003352465E-5	0.0
7	0.0	6.265704400670493E-5	0.0	3.1328522003352465E-5	0.0
8	0.0	6.265704400670493E-5	0.0	3.1328522003352465E-5	0.0
9	0.0	1.5664261001676233E-4	0.0	4.69927830050287E-5	0.0
10-11	0.0	1.5664261001676233E-4	0.0	9.39855660100574E-5	0.0
12-13	0.0	1.5664261001676233E-4	0.0	9.39855660100574E-5	0.0
14-15	0.0	2.506281760268197E-4	0.0	9.39855660100574E-5	0.0
16-17	7.832130500838116E-6	2.6629243702849597E-4	0.0	9.39855660100574E-5	0.0
18-19	1.5664261001676233E-5	2.976209590318484E-4	0.0	9.39855660100574E-5	1.5664261001676233E-5
20-21	1.5664261001676233E-5	3.2894948103520086E-4	0.0	9.39855660100574E-5	1.5664261001676233E-5
22-23	1.5664261001676233E-5	3.2894948103520086E-4	0.0	1.1748195751257174E-4	3.1328522003352465E-5
24-25	1.5664261001676233E-5	3.36781611536039E-4	0.0	1.6447474051760043E-4	4.69927830050287E-5
26-27	1.5664261001676233E-5	3.446137420368771E-4	0.0	2.4279604552598162E-4	4.69927830050287E-5
28-29	1.5664261001676233E-5	3.524458725377152E-4	0.0	4.5426356904861075E-4	4.69927830050287E-5
30-31	1.5664261001676233E-5	4.0727078604358205E-4	0.0	0.0012923015326382892	4.69927830050287E-5
32-33	1.5664261001676233E-5	4.0727078604358205E-4	0.0	0.002968377459817646	6.265704400670493E-5
34-35	1.5664261001676233E-5	5.169206130553156E-4	0.0	0.005208366783057347	7.832130500838117E-5
36-37	1.5664261001676233E-5	6.344025705678875E-4	0.0	0.008724993377933662	7.832130500838117E-5
38-39	1.5664261001676233E-5	6.500668315695636E-4	0.0	0.013941192291491846	8.615343550921929E-5
40-41	1.5664261001676233E-5	6.73563223072078E-4	0.0	0.01946284429458272	9.39855660100574E-5
42-43	1.5664261001676233E-5	6.813953535729162E-4	0.0	0.02489834286216437	9.39855660100574E-5
44-45	1.5664261001676233E-5	7.753809195829736E-4	0.0	0.0313363541338533	1.1748195751257174E-4
46-47	1.5664261001676233E-5	8.458700940905166E-4	0.0	0.03779786179704475	1.4097834901508608E-4
48-49	1.5664261001676233E-5	8.458700940905166E-4	0.0	0.04428286585173871	1.5664261001676233E-4
50-51	1.5664261001676233E-5	0.0010025127041072789	0.0	0.050572066643911714	1.5664261001676233E-4
52-53	1.5664261001676233E-5	0.0010181769651089552	0.0	0.05682210678358053	2.1929965402346725E-4
54-55	1.5664261001676233E-5	0.0010181769651089552	0.0	0.06374571014632144	2.897888285310103E-4
56-57	1.5664261001676233E-5	0.0010338412261106313	0.0	0.07071630629206735	2.976209590318484E-4
58-59	1.5664261001676233E-5	0.0010495054871123076	0.0	0.07734228869577639	2.976209590318484E-4
60-61	1.5664261001676233E-5	0.0010886661396164981	0.0	0.08361582522694773	2.976209590318484E-4
62-63	1.5664261001676233E-5	0.0011043304006181744	0.0	0.08898083462002185	2.976209590318484E-4
64-65	1.5664261001676233E-5	0.0013314621851424797	0.0	0.09468262562463198	2.976209590318484E-4
66-67	1.5664261001676233E-5	0.0013314621851424797	0.0	0.10043140941224717	2.976209590318484E-4
68-69	1.5664261001676233E-5	0.0013784549681475084	0.0	0.10590606863233301	2.976209590318484E-4
70-71	1.5664261001676233E-5	0.0014254477511525371	0.0	0.11073066102084929	3.1328522003352466E-4
72-73	1.5664261001676233E-5	0.0014411120121542134	0.0	0.1143491053122365	3.759422640402296E-4
74-75	1.5664261001676233E-5	0.0014411120121542134	0.0	0.11742713259906587	3.759422640402296E-4
76-77	1.5664261001676233E-5	0.0014567762731558895	0.0	0.12049732775539441	3.759422640402296E-4
78-79	3.1328522003352465E-5	0.0014567762731558895	0.0	0.12278430986163914	3.759422640402296E-4
80-81	3.1328522003352465E-5	0.0014646084036567277	0.0	0.12349703373721542	3.837743945410677E-4
82-83	3.1328522003352465E-5	0.0014724405341575658	0.0	0.12394346517576318	3.916065250419058E-4
84-85	3.1328522003352465E-5	0.0015037690561609184	0.0	0.12419409335179002	3.994386555427439E-4
86-87	3.1328522003352465E-5	0.0015742582306684614	0.0	0.12425675039579671	4.151029165444202E-4
88	3.1328522003352465E-5	0.001738732971186062	0.0	0.12428024678729922	4.229350470452583E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7580	0.0	41.11012	1
CTTATAC	6185	0.0	37.96024	1
GTATCAA	12700	0.0	34.844666	1
TCAACGC	14350	0.0	30.21024	4
ATCAACG	14500	0.0	29.86812	3
TATACAC	8950	0.0	29.734001	3
CAACGCA	14810	0.0	29.300892	5
AACGCAG	15425	0.0	28.3526	6
TATCAAC	15850	0.0	27.621815	2
TTATACA	9225	0.0	27.032803	2
ACGCAGA	17385	0.0	24.9831	7
CGCAGAG	17485	0.0	24.913855	8
ACATCTC	10770	0.0	22.435368	8
ACACATC	10845	0.0	22.359537	6
TACACAT	12365	0.0	22.285667	5
GCAGAGT	21400	0.0	20.295849	9
CACATCT	12210	0.0	19.789429	7
GTACATG	14815	0.0	19.758959	1
TACATGG	14870	0.0	19.512688	2
CATCTCC	12695	0.0	18.965778	9
>>END_MODULE