FastQCFastQC Report
Fri 27 May 2016
SRR2140047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5978724
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87800.053.3728561
GTATCAA159400.037.0919381
CTTATAC56850.032.6902581
ATCAACG193500.030.468213
TCAACGC196050.030.1149024
CAACGCA200150.029.3716815
TATCAAC203400.029.192432
AACGCAG207050.028.4515426
TATACAC81350.026.8338133
ACGCAGA236000.024.9611997
CGCAGAG240750.024.451218
TTATACA83700.024.0664832
TGGTATC36800.021.5288222
GTGGTAT40500.020.914681
GCAGAGT283900.020.7348679
GAGTACT145050.020.4805512-13
ACATCTC92850.020.4221218
TACATGG234550.020.0870692
GTACATG236150.020.0223521
ACACATC95850.019.8710216
Produced by FastQC (version 0.11.5)