##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140047_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5978724
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260749116366636	33.0	32.0	33.0	25.0	34.0
2	31.93198699254222	33.0	32.0	33.0	29.0	34.0
3	32.65467798814596	33.0	33.0	34.0	31.0	34.0
4	32.65929402327319	33.0	33.0	34.0	31.0	34.0
5	33.19343759638344	33.0	33.0	34.0	33.0	34.0
6	37.28184559113282	38.0	38.0	38.0	36.0	38.0
7	37.37943196575055	38.0	38.0	38.0	37.0	38.0
8	37.51353750398915	38.0	38.0	38.0	37.0	38.0
9	37.53124077980519	38.0	38.0	38.0	37.0	38.0
10-11	37.54583528190965	38.0	38.0	38.0	37.5	38.0
12-13	37.567911062628085	38.0	38.0	38.0	38.0	38.0
14-15	37.540420330491926	38.0	38.0	38.0	38.0	38.0
16-17	37.56632635993901	38.0	38.0	38.0	38.0	38.0
18-19	37.483483264990994	38.0	38.0	38.0	37.5	38.0
20-21	37.56009947556646	38.0	38.0	38.0	38.0	38.0
22-23	37.56883636461005	38.0	38.0	38.0	38.0	38.0
24-25	37.568920209575104	38.0	38.0	38.0	38.0	38.0
26-27	37.54603096692411	38.0	38.0	38.0	38.0	38.0
28-29	37.474612242258075	38.0	38.0	38.0	37.0	38.0
30-31	37.51478335354446	38.0	38.0	38.0	37.5	38.0
32-33	37.502640242894394	38.0	38.0	38.0	38.0	38.0
34-35	37.478744060475236	38.0	38.0	38.0	37.0	38.0
36-37	37.457524060498216	38.0	38.0	38.0	37.0	38.0
38-39	37.43899274507632	38.0	38.0	38.0	37.0	38.0
40-41	37.43647666884593	38.0	38.0	38.0	37.0	38.0
42-43	37.37189318649308	38.0	38.0	38.0	37.0	38.0
44-45	37.37714979620378	38.0	38.0	38.0	37.0	38.0
46-47	37.351338934671745	38.0	38.0	38.0	37.0	38.0
48-49	37.31322045493668	38.0	38.0	38.0	37.0	38.0
50-51	37.28091926665623	38.0	38.0	38.0	37.0	38.0
52-53	37.26431416053656	38.0	38.0	38.0	37.0	38.0
54-55	37.25579155851375	38.0	38.0	38.0	37.0	38.0
56-57	37.260472364733445	38.0	38.0	38.0	37.0	38.0
58-59	37.258156855785785	38.0	38.0	38.0	37.0	38.0
60-61	37.25973769914091	38.0	38.0	38.0	37.0	38.0
62-63	37.2645381228529	38.0	38.0	38.0	37.0	38.0
64-65	37.26774249129848	38.0	38.0	38.0	37.0	38.0
66-67	37.27197190929802	38.0	38.0	38.0	37.0	38.0
68-69	37.27203214829045	38.0	38.0	38.0	37.0	38.0
70-71	37.28062695924348	38.0	38.0	38.0	37.0	38.0
72-73	37.25543073378536	38.0	38.0	38.0	37.0	38.0
74-75	37.25566142189952	38.0	38.0	38.0	37.0	38.0
76-77	37.258144197557925	38.0	38.0	38.0	37.0	38.0
78-79	37.24874650134597	38.0	38.0	38.0	37.0	38.0
80-81	37.243961782271654	38.0	38.0	38.0	37.0	38.0
82-83	37.25064654112349	38.0	38.0	38.0	37.0	38.0
84-85	37.240287267776864	38.0	38.0	38.0	37.0	38.0
86-87	37.228056272018925	38.0	38.0	38.0	37.0	38.0
88-89	37.21392769531604	38.0	38.0	38.0	37.0	38.0
90-91	37.20554456829344	38.0	38.0	38.0	36.5	38.0
92-93	37.20510519762239	38.0	38.0	38.0	36.5	38.0
94-95	37.172151985932956	38.0	38.0	38.0	36.0	38.0
96-97	37.186344824786076	38.0	38.0	38.0	36.0	38.0
98-99	37.18486474253783	38.0	38.0	38.0	36.0	38.0
100	35.70630982952784	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	21.0
20	50.0
21	154.0
22	396.0
23	942.0
24	1934.0
25	3703.0
26	6716.0
27	10558.0
28	16667.0
29	24982.0
30	34994.0
31	47565.0
32	61275.0
33	80463.0
34	114667.0
35	203036.0
36	617811.0
37	4752786.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.870911585816636	21.253063362684077	14.333192166087613	23.54283288541167
2	14.21432039691552	21.24507606415639	42.733131249369634	21.807472289558454
3	17.65584094532546	27.146076654483466	30.853874505663747	24.34420789452733
4	11.952533684445042	18.717104184772538	39.65294266803418	29.67741946274824
5	12.760000307786704	38.22904884206962	35.90954148814202	13.101409362001656
6	28.484249996278454	38.58352413577741	19.548205905065938	13.384019962878199
7	25.556289268412456	33.38070130014364	23.537614380593585	17.525395050850314
8	23.70183795132137	36.98178306333695	21.945225083220127	17.37115390212156
9	25.405944253907403	17.046673072725493	21.76925978241144	35.778122890955665
10-11	23.529167762218158	27.31218233188219	29.409711838178183	19.748938067721475
12-13	24.5820847391517	24.91148612981633	29.444669130068558	21.061760000963417
14-15	21.81359266626123	26.219666604446033	27.23147949294866	24.735261236344076
16-17	21.064009644867365	29.456327136024342	27.94275166406745	21.53691155504084
18-19	21.142387573000526	28.349251445626187	29.84306517577998	20.6652958055933
20-21	22.150843399127297	27.356295654012335	29.674631322315488	20.81822962454488
22-23	21.77644084251008	27.562659362289903	29.45485133768982	21.20604845751019
24-25	21.46242637460281	27.73663502076532	29.697221722260547	21.10371688237132
26-27	21.411739317542228	27.906099846388244	29.646032170225077	21.036128665844448
28-29	21.224028145086958	27.766708300618554	29.830149206177087	21.179114348117402
30-31	21.51453986748666	27.834660672214994	29.582895377108574	21.067904083189767
32-33	21.653783141252163	27.614551759168464	29.58860327821624	21.14306182136313
34-35	21.47006608088632	27.678508733746888	29.672377354309436	21.17904783105736
36-37	21.295943395867415	27.85508340813773	29.628359908450406	21.22061328754444
38-39	21.19028079670908	27.653072192430766	29.83592795709622	21.32071905376393
40-41	21.435150745688382	27.513754224941938	29.826909667757157	21.224185361612527
42-43	21.52220168727904	27.518545096159198	29.61516039765968	21.344092818902084
44-45	21.435742644188526	27.760493707887758	29.448799914007584	21.354963733916133
46-47	21.606095624351138	27.59148586995997	29.132029907175532	21.67038859851336
48-49	21.5115048947633	27.642020201016155	29.183970040697393	21.66250486352315
50-51	21.404880998806433	27.824871028225555	29.1803666615733	21.589881311394713
52-53	21.551888234096815	27.772517337394646	29.211690711160447	21.46390371734809
54-55	21.548199408871305	27.81904863459844	28.98995919699095	21.642792759539308
56-57	21.745776338663582	27.988784748170247	28.705218627115237	21.560220286050935
58-59	21.424078325106947	28.291388099782523	29.022238831292952	21.262294743817574
60-61	21.680369380326862	28.462565284599005	28.62889864032403	21.2281666947501
62-63	21.424360878891	28.64136078036062	28.59201979702741	21.342258543720963
64-65	21.409007676455406	28.603328014344235	28.56376576006517	21.42389854913519
66-67	21.491616158966984	28.59753101569712	28.49283643441109	21.418016390924805
68-69	21.528459798959776	28.497633481063	28.513827283157106	21.46007943682012
70-71	21.544665321951435	28.19088713211088	28.646015668548095	21.61843187738959
72-73	21.64934985918099	28.008425507222473	28.68931470094126	21.652909932655277
74-75	21.620564304586054	28.069678220552852	28.620541220609496	21.689216254251594
76-77	21.706901607300235	28.004912828750534	28.537937886809182	21.75024767714005
78-79	21.70999317985635	28.03617827732762	28.49523000796637	21.758598534849664
80-81	21.666840831745063	28.021393154354772	28.51352789098508	21.798238122915084
82-83	21.756786031556103	28.01752655623503	28.517337866846503	21.708349545362367
84-85	21.873132597110335	27.988175649308534	28.419348917922278	21.719342835658857
86-87	21.7462319300441	27.99769241172375	28.429352540296225	21.826723117935924
88-89	21.83361306610626	27.94933692754686	28.42444923202776	21.79260077431912
90-91	21.86363235797733	27.955321994647832	28.42525982783373	21.755785819541106
92-93	21.861128059589195	28.0049312920491	28.383697787083257	21.75024286127845
94-95	21.82475565370536	28.088340768112403	28.33348156402663	21.753422014155614
96-97	21.87931751531561	28.187297861948103	28.38728932792473	21.546095294811558
98-99	22.94370451091914	30.070077017898328	28.65219557155249	18.334022899630046
100	21.757685320094254	31.016098091063093	22.630123767169163	24.596092821673487
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1066.0
1	1054.0
2	1624.5
3	2515.5
4	3239.0
5	4091.0
6	4800.0
7	6019.5
8	7791.5
9	9177.0
10	9776.5
11	10252.5
12	11450.5
13	13626.5
14	17335.5
15	21246.5
16	23775.5
17	24533.5
18	23569.5
19	21612.5
20	19679.0
21	18944.5
22	20566.0
23	24882.5
24	32034.5
25	42158.5
26	54919.0
27	69027.5
28	83785.5
29	99447.0
30	114419.0
31	129487.5
32	145905.0
33	161198.5
34	176206.5
35	190004.5
36	204237.5
37	218069.0
38	227620.0
39	235348.5
40	243354.0
41	249661.0
42	256405.0
43	271620.5
44	276153.0
45	270765.5
46	265995.5
47	257055.0
48	249459.5
49	241475.0
50	233230.5
51	222311.0
52	208798.5
53	194122.0
54	178133.0
55	161568.5
56	145503.5
57	129077.5
58	112262.0
59	96446.5
60	80294.5
61	64300.5
62	50019.5
63	38477.0
64	28984.0
65	21272.0
66	15331.5
67	11197.0
68	8311.0
69	5912.0
70	4084.0
71	2805.5
72	2026.5
73	1415.0
74	930.0
75	614.0
76	394.5
77	245.0
78	164.0
79	97.5
80	57.5
81	39.0
82	26.0
83	22.0
84	15.5
85	10.0
86	8.0
87	5.0
88	3.0
89	2.0
90	3.0
91	3.5
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.5124551660187025E-4
3	0.0
4	0.0
5	0.009333095155421123
6	4.516013784881188E-4
7	0.0
8	3.345195396208288E-5
9	1.5053379282937296E-4
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	8.363664161414704E-5
26-27	0.0
28-29	6.526179770147948E-4
30-31	2.5963233883259754E-4
32-33	0.0015445385745571016
34-35	3.367768201903092E-4
36-37	0.0014967702741761802
38-39	7.829468058280859E-4
40-41	8.312064730589702E-4
42-43	1.7260253993267637E-4
44-45	1.0444412356888703E-4
46-47	5.800836199327279E-4
48-49	0.0016596053564960991
50-51	0.0018539992600124693
52-53	0.001770680760803705
54-55	0.0014673192014393215
56-57	0.0030546302213705244
58-59	4.459899648541325E-4
60-61	1.8763982685347337E-5
62-63	0.002065305724932731
64-65	0.0026795196846128772
66-67	0.003174221911595791
68-69	8.620945547266931E-4
70-71	0.004890415994854449
72-73	0.0012269029239208558
74-75	0.004047158960506541
76-77	3.495638574001538E-4
78-79	0.0011129285476989734
80-81	0.0
82-83	3.1936134543954124E-5
84-85	0.001001141301083235
86-87	0.0
88-89	1.1018884274057724E-5
90-91	0.0031233024014849075
92-93	5.193659129312402E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	40.0
20-21	116.0
22-23	264.0
24-25	385.0
26-27	1310.0
28-29	3677.0
30-31	12711.0
32-33	16502.0
34-35	22752.0
36-37	35489.0
38-39	40792.0
40-41	40120.0
42-43	46534.0
44-45	54993.0
46-47	55935.0
48-49	52346.0
50-51	48599.0
52-53	47424.0
54-55	51120.0
56-57	53182.0
58-59	51961.0
60-61	52031.0
62-63	52137.0
64-65	55958.0
66-67	61546.0
68-69	64521.0
70-71	69331.0
72-73	56734.0
74-75	53425.0
76-77	55538.0
78-79	56255.0
80-81	54927.0
82-83	52409.0
84-85	52479.0
86-87	53854.0
88-89	55094.0
90-91	54488.0
92-93	53592.0
94-95	61280.0
96-97	176041.0
98-99	370508.0
100-101	3780324.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.426584937891
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.30127424167306	80.73459881668981
2	6.459441542645358	11.42372712444143
3	1.4127265912485092	3.7476776374516048
4	0.4393090616966004	1.5538640023239851
5	0.1777476818759889	0.785881024446018
6	0.07778859942910109	0.4127148116770191
7	0.0433528970231803	0.26834840416467265
8	0.024359056595742064	0.17231905496562236
9	0.017107761347715893	0.13615028207198712
>10	0.04548986343899308	0.5880162405350678
>50	8.989502357088719E-4	0.0527239230140164
>100	4.001389088560012E-4	0.06435555159923696
>500	1.0361388116074056E-4	0.05962312661972922
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.017793094312432E-5	0.0
3	0.0	0.0	0.0	5.017793094312432E-5	0.0
4	0.0	1.0035586188624863E-4	0.0	6.690390792416576E-5	0.0
5	0.0	1.3380781584833152E-4	0.0	6.690390792416576E-5	0.0
6	6.690390792416576E-5	1.3380781584833152E-4	0.0	6.690390792416576E-5	0.0
7	6.690390792416576E-5	1.3380781584833152E-4	0.0	1.3380781584833152E-4	0.0
8	6.690390792416576E-5	1.8398574679145583E-4	0.0	1.3380781584833152E-4	0.0
9	6.690390792416576E-5	3.177935626397874E-4	0.0	1.672597698104144E-4	0.0
10-11	6.690390792416576E-5	3.177935626397874E-4	0.0	2.0071172377249727E-4	0.0
12-13	6.690390792416576E-5	3.345195396208288E-4	0.0	2.592526432061423E-4	0.0
14-15	6.690390792416576E-5	4.348754015070774E-4	0.0	3.094305741492666E-4	0.0
16-17	1.1708183886729007E-4	4.5160137848811887E-4	0.0	3.5124551660187025E-4	0.0
18-19	1.1708183886729007E-4	5.268682749028054E-4	0.0	3.5124551660187025E-4	0.0
20-21	1.1708183886729007E-4	5.854091943364504E-4	0.0	3.5960850509239096E-4	0.0
22-23	1.1708183886729007E-4	6.690390792416576E-4	0.0	3.7633448207343237E-4	0.0
24-25	1.1708183886729007E-4	6.85765056222699E-4	0.0	4.265124130165567E-4	0.0
26-27	1.1708183886729007E-4	6.941280447132197E-4	0.0	4.934163209407225E-4	0.0
28-29	1.1708183886729007E-4	7.024910332037405E-4	0.0	6.941280447132197E-4	0.0
30-31	1.1708183886729007E-4	7.192170101847819E-4	0.0	0.0014718859743316466	0.0
32-33	1.1708183886729007E-4	7.192170101847819E-4	0.0	0.003203024591869436	0.0
34-35	1.1708183886729007E-4	8.028468950899892E-4	0.0	0.005753736081478255	0.0
36-37	1.1708183886729007E-4	8.864767799951963E-4	0.0	0.009951956303719657	0.0
38-39	1.1708183886729007E-4	9.032027569762377E-4	0.0	0.015964945028404055	0.0
40-41	1.1708183886729007E-4	9.533806879193621E-4	0.0	0.022479713062519696	0.0
42-43	1.1708183886729007E-4	0.0010119216073530071	0.0	0.02893594017720169	0.0
44-45	1.1708183886729007E-4	0.0011290034462202972	0.0	0.03650444476112294	0.0
46-47	1.1708183886729007E-4	0.0012042703426349837	0.0	0.04454963968900387	0.0
48-49	1.1708183886729007E-4	0.0012042703426349837	0.0	0.0530380730068824	0.0
50-51	1.5053379282937296E-4	0.001312989193011753	0.0	0.06095782310740552	0.0
52-53	1.5053379282937296E-4	0.0013464411469738358	0.0	0.0685765056222699	0.0
54-55	1.5053379282937296E-4	0.0013966190779169602	0.0	0.07597775043638075	0.0
56-57	1.5053379282937296E-4	0.0014384340203695638	0.0	0.08215799893087555	0.0
58-59	1.5053379282937296E-4	0.0014635229858411258	0.0	0.08867276696499118	0.0
60-61	1.672597698104144E-4	0.0014802489628221675	0.0	0.09512899407967318	0.0
62-63	2.0071172377249727E-4	0.0015053379282937295	0.0	0.1009914490115282	0.0
64-65	2.0071172377249727E-4	0.0015722418362178953	0.0	0.10737240922979552	0.0
66-67	2.0071172377249727E-4	0.0015889678131989367	0.0	0.1137031915171197	0.0
68-69	2.0071172377249727E-4	0.0016224197671610196	0.0	0.11980817311519984	0.0
70-71	2.174377007535387E-4	0.0016307827556515404	0.0	0.1250434039102658	0.0
72-73	2.174377007535387E-4	0.001647508732632582	0.0	0.12962632160307117	0.0
74-75	2.174377007535387E-4	0.0016642347096136232	0.0	0.1326286344711681	0.0
76-77	2.174377007535387E-4	0.0017478645945188304	0.0	0.13584002205152806	0.0
78-79	2.174377007535387E-4	0.0017813165484809133	0.0	0.1380729399784971	0.0
80-81	2.174377007535387E-4	0.001789679536971434	0.0	0.13896777974698282	0.0
82-83	2.258006892440594E-4	0.001798042525461955	0.0	0.1395364629643382	0.0
84-85	2.3416367773458014E-4	0.0018064055139524755	0.0	0.1397789896305633	0.0
86-87	2.4252666622510087E-4	0.001823131490933517	0.0	0.13987098250395902	0.0
88	2.508896547156216E-4	0.0019234873528197656	0.0	0.1398960714694306	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8780	0.0	53.372856	1
GTATCAA	15940	0.0	37.091938	1
CTTATAC	5685	0.0	32.690258	1
ATCAACG	19350	0.0	30.46821	3
TCAACGC	19605	0.0	30.114902	4
CAACGCA	20015	0.0	29.371681	5
TATCAAC	20340	0.0	29.19243	2
AACGCAG	20705	0.0	28.451542	6
TATACAC	8135	0.0	26.833813	3
ACGCAGA	23600	0.0	24.961199	7
CGCAGAG	24075	0.0	24.45121	8
TTATACA	8370	0.0	24.066483	2
TGGTATC	3680	0.0	21.528822	2
GTGGTAT	4050	0.0	20.91468	1
GCAGAGT	28390	0.0	20.734867	9
GAGTACT	14505	0.0	20.48055	12-13
ACATCTC	9285	0.0	20.422121	8
TACATGG	23455	0.0	20.087069	2
GTACATG	23615	0.0	20.022352	1
ACACATC	9585	0.0	19.871021	6
>>END_MODULE