FastQCFastQC Report
Fri 27 May 2016
SRR2140046_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140046_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6468830
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC148300.058.1345521
GGTATCA93400.051.662651
TATACAC183700.049.200033
TTATACA190150.046.873722
GTATCAA186350.036.7833671
TACACAT259400.034.5369075
ATACACA286800.032.1672134
ACACATC278000.031.6836266
ATCAACG246650.030.7978573
TATCAAC252250.030.3949622
TCAACGC250850.030.3154324
CAACGCA254950.029.8442545
AACGCAG262250.029.0267056
CACATCT305000.028.9061687
CATCTCC324650.027.1309039
ACGCAGA296650.025.5904947
TGGTATC41100.025.4476032
CGCAGAG302000.025.1371568
GTGGTAT39050.024.9694981
GCAGAGT339300.022.3369319
Produced by FastQC (version 0.11.5)