##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140046_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6468830
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.746482748812383	18.0	18.0	30.0	18.0	33.0
2	31.07008516223181	32.0	31.0	33.0	27.0	33.0
3	31.820579610223177	33.0	31.0	33.0	29.0	33.0
4	32.593461414197	33.0	33.0	33.0	31.0	34.0
5	32.978190182768756	33.0	33.0	34.0	32.0	34.0
6	36.92270920707454	38.0	37.0	38.0	35.0	38.0
7	37.166629359559614	38.0	38.0	38.0	36.0	38.0
8	37.08858479817834	38.0	38.0	38.0	36.0	38.0
9	37.34115288236049	38.0	38.0	38.0	37.0	38.0
10-11	37.41211888394037	38.0	38.0	38.0	37.0	38.0
12-13	37.44642493310228	38.0	38.0	38.0	37.0	38.0
14-15	37.41754521296741	38.0	38.0	38.0	37.0	38.0
16-17	37.416109481931045	38.0	38.0	38.0	37.0	38.0
18-19	37.386537982911904	38.0	38.0	38.0	37.0	38.0
20-21	37.47689424778911	38.0	38.0	38.0	37.0	38.0
22-23	37.50339113456328	38.0	38.0	38.0	37.0	38.0
24-25	37.511298745556616	38.0	38.0	38.0	37.0	38.0
26-27	37.49080848488269	38.0	38.0	38.0	37.0	38.0
28-29	37.414454877238995	38.0	38.0	38.0	37.0	38.0
30-31	37.459714468383794	38.0	38.0	38.0	37.0	38.0
32-33	37.44551453809322	38.0	38.0	38.0	37.0	38.0
34-35	37.41090195157609	38.0	38.0	38.0	37.0	38.0
36-37	37.388295580952025	38.0	38.0	38.0	37.0	38.0
38-39	37.33871230811156	38.0	38.0	38.0	37.0	38.0
40-41	37.36302481399988	38.0	38.0	38.0	37.0	38.0
42-43	37.296849801428394	38.0	38.0	38.0	37.0	38.0
44-45	37.29120389380164	38.0	38.0	38.0	37.0	38.0
46-47	37.25335738316821	38.0	38.0	38.0	37.0	38.0
48-49	37.20750373154448	38.0	38.0	38.0	37.0	38.0
50-51	37.16677850349782	38.0	38.0	38.0	37.0	38.0
52-53	37.14468797498159	38.0	38.0	38.0	37.0	38.0
54-55	37.12449781204094	38.0	38.0	38.0	37.0	38.0
56-57	37.1247513965733	38.0	38.0	38.0	37.0	38.0
58-59	37.116065759265126	38.0	38.0	38.0	36.0	38.0
60-61	37.0955804779129	38.0	38.0	38.0	36.0	38.0
62-63	37.089103273367954	38.0	38.0	38.0	36.0	38.0
64-65	37.085532569184494	38.0	38.0	38.0	36.0	38.0
66-67	37.07755333766696	38.0	38.0	38.0	36.0	38.0
68-69	37.076402611119136	38.0	38.0	38.0	36.0	38.0
70-71	37.079073818635194	38.0	38.0	38.0	36.0	38.0
72-73	37.03186295104351	38.0	38.0	38.0	36.0	38.0
74-75	37.03711700665124	38.0	38.0	38.0	36.0	38.0
76-77	37.04395818568257	38.0	38.0	38.0	36.0	38.0
78-79	37.039878129467006	38.0	38.0	38.0	36.0	38.0
80-81	37.04214509214951	38.0	38.0	38.0	36.0	38.0
82-83	37.05197990141835	38.0	38.0	38.0	36.0	38.0
84-85	37.04309507805766	38.0	38.0	38.0	36.0	38.0
86-87	37.03198498811177	38.0	38.0	38.0	36.0	38.0
88-89	37.02071594762283	38.0	38.0	38.0	36.0	38.0
90-91	37.01524828053304	38.0	38.0	38.0	36.0	38.0
92-93	37.01353349027883	38.0	38.0	38.0	36.0	38.0
94-95	36.979574902800294	38.0	38.0	38.0	36.0	38.0
96-97	36.99713103013036	38.0	38.0	38.0	36.0	38.0
98-99	36.99494106486995	38.0	38.0	38.0	36.0	38.0
100	35.348779003525465	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	5.0
18	14.0
19	31.0
20	98.0
21	219.0
22	601.0
23	1356.0
24	3032.0
25	5659.0
26	9502.0
27	15694.0
28	24696.0
29	37428.0
30	50575.0
31	65399.0
32	83211.0
33	109919.0
34	161394.0
35	294063.0
36	1003179.0
37	4602754.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.87065512619747	19.379022172479413	19.57564196307524	24.174680738247876
2	13.579854718255755	20.854593039523497	43.11040206776826	22.455150174452488
3	17.135447986730213	26.984895259266363	32.107336257097494	23.772320496905934
4	11.533553981168156	19.256326105338985	38.017632245707496	31.19248766778536
5	12.381670453294614	37.97508060755899	35.98490563937533	13.658343299771069
6	28.17149294203507	38.32354453869355	18.956695308288673	14.5482672109827
7	24.766565205763637	33.44830518038038	22.466906689463165	19.31822292439282
8	23.873737251941154	36.52088137140144	21.882541938045037	17.72283943861237
9	24.590682437095136	16.73845723854257	20.769766832137442	37.90109349222485
10-11	23.47794732586882	26.867532459501952	28.902707290190033	20.751812924439193
12-13	24.726071020570952	24.26253279186499	28.705708760316778	22.305687427247275
14-15	22.01430552356454	25.722765940672428	26.193399733800394	26.069528801962644
16-17	21.56173527515795	29.46930897859427	26.7148541544607	22.254101591787077
18-19	21.503046145902736	27.35970028583221	29.007795845616595	22.129457722648453
20-21	22.051224060161534	26.2679869488823	29.102018359743	22.57877063121317
22-23	21.480261022011558	27.269974738807935	29.09913858407463	22.150625655105884
24-25	21.47688979534978	27.46632421595947	29.63027584191103	21.426510146779716
26-27	21.24275531547474	27.344770401072598	29.81025751687846	21.602216766574198
28-29	20.272755076420708	26.40683981546842	30.055764477036504	23.264640631074368
30-31	20.672770128444366	26.268079914056518	30.553738219324394	22.505411738174715
32-33	20.659538746660996	26.8019171668331	30.47725819892877	22.061285887577135
34-35	20.770162571040448	27.6551897516495	30.74773143054398	20.82691624676607
36-37	21.130017987874016	27.046743970923192	29.515542301250196	22.307695739952596
38-39	21.23746166051877	26.47158856551205	30.144154794199757	22.146794979769428
40-41	21.34423352572346	26.338623194868404	30.441530536694515	21.875612742713617
42-43	21.923966286183383	26.144730335873977	30.44013749245888	21.49116588548376
44-45	21.14527171482155	26.938468533999156	30.171574905209564	21.74468484596973
46-47	22.202429074523877	26.098084782591325	29.07635052012804	22.623135622756756
48-49	21.79629470542891	26.17368880259951	29.408546651490614	22.621469840480966
50-51	21.240862652864152	26.717147846589423	29.821341351128115	22.220648149418306
52-53	21.35732227802249	27.157822476678366	30.29405346111027	21.19080178418887
54-55	21.29698285262181	27.384242940632507	29.2181823407194	22.10059186602628
56-57	22.031135335290163	28.1812525933464	28.03205150179166	21.755560569571777
58-59	20.809176238513505	29.23576002341074	29.311891386839307	20.64317235123645
60-61	21.619617860455513	30.34743379510042	27.4289353395432	20.604013004900867
62-63	20.796852313828214	30.961323109178668	27.44993148261371	20.791893094379407
64-65	20.784593916450078	30.721359725131727	27.599491571480545	20.894554786937654
66-67	21.016908241209187	30.399514928471056	27.507256923944283	21.076319906375474
68-69	21.129213521460937	29.78298612231145	27.862958724157323	21.22484163207029
70-71	21.264851524412194	29.001080435113774	28.145630376305103	21.58843766416893
72-73	21.69244079702119	27.893556598966057	28.54196077963287	21.87204182437988
74-75	21.685029657673038	27.870828557130757	28.477045441327935	21.967096343868274
76-77	21.782257179973367	27.815419739489357	28.347856360952196	22.054466719585086
78-79	21.769998080420045	27.79608525165215	28.35314177890103	22.08077488902677
80-81	21.765010688199173	27.81327753816405	28.340330389987084	22.0813813836497
82-83	21.882087057112887	27.730590294079988	28.3941397719062	21.99318287690092
84-85	22.085740786833743	27.722608631088306	28.214913927927103	21.976736654150844
86-87	21.85888958541162	27.769195044182915	28.302284849103327	22.06963052130214
88-89	21.969790491342167	27.710444001958322	28.274462830182795	22.045302676516712
90-91	22.04015358484002	27.64776529453295	28.26629984344783	22.045781277179195
92-93	22.03793314218447	27.65883335928667	28.268460049614735	22.034773448914123
94-95	21.932835478793887	27.78817460854531	28.231877825535527	22.047112087125274
96-97	21.998738005420563	27.91850670558057	28.260124000343072	21.822631288655796
98-99	23.059101552805082	29.773703627768004	28.48574227344285	18.68145254598406
100	21.901227770854263	30.712986371973972	22.439506055282486	24.94627980188928
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2164.0
1	2218.0
2	2897.5
3	3705.5
4	4465.0
5	5520.5
6	6534.0
7	9352.0
8	12186.5
9	12910.0
10	13023.0
11	13497.5
12	14991.0
13	17918.5
14	23793.5
15	30270.0
16	33776.0
17	34162.0
18	31954.5
19	28497.0
20	25190.5
21	23080.0
22	23506.5
23	27186.5
24	33783.5
25	43235.5
26	55792.5
27	69769.5
28	83572.0
29	98377.5
30	113251.0
31	128115.5
32	144297.5
33	159866.0
34	175682.0
35	191350.5
36	206610.5
37	219778.0
38	228841.0
39	236217.0
40	243100.5
41	247531.5
42	262171.5
43	324781.0
44	352799.0
45	342585.0
46	334607.5
47	296260.0
48	266558.0
49	251568.0
50	242996.5
51	234587.0
52	223351.5
53	210556.5
54	197250.0
55	182266.0
56	166432.0
57	149378.5
58	132095.0
59	114758.0
60	96196.5
61	78057.0
62	60768.5
63	46265.5
64	35435.0
65	26522.5
66	19522.0
67	14766.0
68	10988.5
69	8047.5
70	5889.0
71	4169.5
72	3003.5
73	2181.0
74	1487.0
75	1005.5
76	678.5
77	400.5
78	251.5
79	161.0
80	109.5
81	84.5
82	59.5
83	35.5
84	21.0
85	13.5
86	12.0
87	10.0
88	7.5
89	5.0
90	3.5
91	3.0
92	3.5
93	3.5
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.0917492034881116E-4
3	0.0
4	0.0
5	0.007018270691918013
6	6.028910946801817E-4
7	0.0
8	3.0917492034881116E-5
9	2.1642244424416781E-4
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.0822684005534102E-4
26-27	7.731683371484035E-6
28-29	5.723843427821097E-4
30-31	2.5556225438434473E-4
32-33	0.0015451015931472647
34-35	3.038279517352939E-4
36-37	0.001291597057413133
38-39	7.80321028800467E-4
40-41	7.941519287289941E-4
42-43	1.3602005479687926E-4
44-45	1.1301139776452155E-4
46-47	5.055857439172326E-4
48-49	0.0013837151714567234
50-51	0.0016211578558815607
52-53	0.0016348285894799534
54-55	0.001208737770933711
56-57	0.002916230511138466
58-59	3.9579892141352186E-4
60-61	1.738111599105255E-5
62-63	0.0018497634460846364
64-65	0.0023551019090063555
66-67	0.0030329631488639876
68-69	7.833775757339488E-4
70-71	0.004674064265004802
72-73	0.0012802353322812303
74-75	0.004237396278767294
76-77	2.855302242111317E-4
78-79	0.001005578972049385
80-81	1.0071329181797159E-5
82-83	2.037333525657618E-5
84-85	8.959163514826077E-4
86-87	0.0
88-89	2.106630471946318E-5
90-91	0.0028350828068001914
92-93	5.284100755507785E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	62.0
20-21	255.0
22-23	482.0
24-25	828.0
26-27	2004.0
28-29	5103.0
30-31	15829.0
32-33	20140.0
34-35	26990.0
36-37	41490.0
38-39	48100.0
40-41	46273.0
42-43	52307.0
44-45	61522.0
46-47	62147.0
48-49	57784.0
50-51	51840.0
52-53	47873.0
54-55	50995.0
56-57	52499.0
58-59	53977.0
60-61	77601.0
62-63	71246.0
64-65	72760.0
66-67	92245.0
68-69	102703.0
70-71	138905.0
72-73	68713.0
74-75	54110.0
76-77	55730.0
78-79	57268.0
80-81	57032.0
82-83	53573.0
84-85	53160.0
86-87	54710.0
88-89	55324.0
90-91	55203.0
92-93	54583.0
94-95	62204.0
96-97	183104.0
98-99	401455.0
100-101	3948701.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.33804349864607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.72488535610627	76.56946169430428
2	7.128693523195485	12.166975159419456
3	1.8288483070240942	4.682110091317422
4	0.6464158240769655	2.206554468531729
5	0.2814170155413484	1.2007788756763373
6	0.13972534521113467	0.7154332552494689
7	0.0830544764439534	0.49613945664719605
8	0.04434326142363401	0.3027333737793531
9	0.03179299489509832	0.244183678317912
>10	0.08852044254065967	1.128470020774557
>50	0.0013847752981513476	0.08307598165798463
>100	8.067585881806631E-4	0.12667305509144328
>500	8.393974334759799E-5	0.05226787206498968
>1k	2.7979911657642807E-5	0.025143017167889904
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5458746017440558E-5	0.0
2	0.0	0.0	0.0	3.0917492034881116E-5	3.0917492034881116E-5
3	0.0	0.0	0.0	3.0917492034881116E-5	3.0917492034881116E-5
4	0.0	0.0	0.0	3.0917492034881116E-5	3.0917492034881116E-5
5	0.0	0.0	0.0	3.0917492034881116E-5	3.0917492034881116E-5
6	0.0	0.0	0.0	3.0917492034881116E-5	3.0917492034881116E-5
7	0.0	0.0	0.0	6.183498406976223E-5	3.0917492034881116E-5
8	1.5458746017440558E-5	0.0	0.0	9.275247610464335E-5	3.0917492034881116E-5
9	1.5458746017440558E-5	6.183498406976223E-5	0.0	1.2366996813952447E-4	3.0917492034881116E-5
10-11	1.5458746017440558E-5	6.183498406976223E-5	0.0	1.855049522092867E-4	3.0917492034881116E-5
12-13	2.3188119026160837E-5	6.183498406976223E-5	0.0	1.855049522092867E-4	3.0917492034881116E-5
14-15	1.0048184911336362E-4	8.502310309592306E-5	0.0	2.0096369822672727E-4	3.8646865043601396E-5
16-17	1.855049522092867E-4	9.275247610464335E-5	0.0	2.241518172528881E-4	4.6376238052321675E-5
18-19	2.1642244424416781E-4	9.275247610464335E-5	0.0	2.3188119026160836E-4	4.6376238052321675E-5
20-21	2.1642244424416781E-4	9.275247610464335E-5	0.0	2.3188119026160836E-4	7.729373008720279E-5
22-23	2.859868013226503E-4	1.0821122212208391E-4	0.0	2.7825742831393E-4	1.0048184911336364E-4
24-25	2.937161743313706E-4	1.3912871415696504E-4	0.0	3.246336663662517E-4	1.0821122212208391E-4
26-27	3.091749203488111E-4	1.5458746017440558E-4	0.0	4.019273964534545E-4	1.0821122212208391E-4
28-29	4.0965676946217475E-4	1.5458746017440558E-4	0.0	7.49749181845867E-4	1.0821122212208391E-4
30-31	4.4830363450577615E-4	2.0096369822672724E-4	0.0	0.002164224442441678	1.0821122212208391E-4
32-33	4.4830363450577615E-4	2.0096369822672724E-4	0.0	0.005093656812746664	1.0821122212208391E-4
34-35	4.4830363450577615E-4	2.0869307123544753E-4	0.0	0.008510039682601027	1.2366996813952447E-4
36-37	4.4830363450577615E-4	2.1642244424416781E-4	0.0	0.013704178344461054	1.3139934114824472E-4
38-39	4.4830363450577615E-4	2.1642244424416781E-4	0.0	0.02524413224648043	1.39128714156965E-4
40-41	4.4830363450577615E-4	2.3188119026160836E-4	0.0	0.037479729719284635	1.39128714156965E-4
42-43	4.4830363450577615E-4	2.550693092877692E-4	0.0	0.046268026830199586	1.39128714156965E-4
44-45	4.4830363450577615E-4	3.091749203488111E-4	0.0	0.05626983550348363	1.39128714156965E-4
46-47	4.4830363450577615E-4	3.4009241238369225E-4	0.0	0.06724554517586642	1.39128714156965E-4
48-49	4.4830363450577615E-4	3.4009241238369225E-4	0.0	0.07744058817436847	1.39128714156965E-4
50-51	4.4830363450577615E-4	4.0965676946217475E-4	0.0	0.08723370377641707	1.39128714156965E-4
52-53	4.4830363450577615E-4	4.3284488848833563E-4	0.0	0.10841991519331935	1.468580871656853E-4
54-55	4.4830363450577615E-4	4.4830363450577615E-4	0.0	0.13276744017078823	1.5458746017440558E-4
56-57	4.4830363450577615E-4	4.4830363450577615E-4	0.0	0.16421052957026233	1.5458746017440558E-4
58-59	4.4830363450577615E-4	4.4830363450577615E-4	0.0	0.18967881363399564	1.5458746017440558E-4
60-61	4.4830363450577615E-4	4.4830363450577615E-4	0.0	0.200499935846204	1.5458746017440558E-4
62-63	4.4830363450577615E-4	4.637623805232167E-4	0.0	0.21391812738934243	1.5458746017440558E-4
64-65	4.4830363450577615E-4	5.410561106104195E-4	0.0	0.220534470684807	1.5458746017440558E-4
66-67	4.4830363450577615E-4	5.410561106104195E-4	0.0	0.22625420671126	1.5458746017440558E-4
68-69	4.4830363450577615E-4	5.410561106104195E-4	0.0	0.23209761270585255	1.5458746017440558E-4
70-71	4.4830363450577615E-4	5.410561106104195E-4	0.0	0.23733039823275615	1.6231683318312587E-4
72-73	4.4830363450577615E-4	5.410561106104195E-4	0.0	0.24158155338755233	2.0096369822672724E-4
74-75	4.4830363450577615E-4	5.565148566278601E-4	0.0	0.24498247751138924	2.0096369822672724E-4
76-77	4.4830363450577615E-4	5.951617216714614E-4	0.0	0.24840658975425234	2.0096369822672724E-4
78-79	4.4830363450577615E-4	6.028910946801817E-4	0.0	0.25035439175244983	2.0096369822672724E-4
80-81	4.4830363450577615E-4	6.028910946801817E-4	0.0	0.25094182410111254	2.0869307123544753E-4
82-83	4.4830363450577615E-4	6.028910946801817E-4	0.0	0.2515060683307492	2.3188119026160836E-4
84-85	4.4830363450577615E-4	6.028910946801817E-4	0.0	0.2517224907749933	2.3188119026160836E-4
86-87	4.4830363450577615E-4	6.183498406976223E-4	0.0	0.25179978450508056	2.3188119026160836E-4
88	4.4830363450577615E-4	7.111023168022657E-4	0.0	0.25180751387808925	2.3188119026160836E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	14830	0.0	58.134552	1
GGTATCA	9340	0.0	51.66265	1
TATACAC	18370	0.0	49.20003	3
TTATACA	19015	0.0	46.87372	2
GTATCAA	18635	0.0	36.783367	1
TACACAT	25940	0.0	34.536907	5
ATACACA	28680	0.0	32.167213	4
ACACATC	27800	0.0	31.683626	6
ATCAACG	24665	0.0	30.797857	3
TATCAAC	25225	0.0	30.394962	2
TCAACGC	25085	0.0	30.315432	4
CAACGCA	25495	0.0	29.844254	5
AACGCAG	26225	0.0	29.026705	6
CACATCT	30500	0.0	28.906168	7
CATCTCC	32465	0.0	27.130903	9
ACGCAGA	29665	0.0	25.590494	7
TGGTATC	4110	0.0	25.447603	2
CGCAGAG	30200	0.0	25.137156	8
GTGGTAT	3905	0.0	24.969498	1
GCAGAGT	33930	0.0	22.336931	9
>>END_MODULE