FastQCFastQC Report
Fri 27 May 2016
SRR2140045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6916870
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69550.045.7518041
CTTATAC61750.036.8575131
GTATCAA125550.033.5536231
ATCAACG158200.030.5098953
TCAACGC159050.030.4008394
CAACGCA164000.029.4308825
TATCAAC168400.028.9428232
AACGCAG170000.028.3648366
TATACAC95550.027.3245963
TTATACA94400.025.883762
ACGCAGA197650.024.3533237
CGCAGAG198900.024.2002748
ACACATC103800.022.586286
ACATCTC105700.022.4240238
TACACAT127400.020.6619785
GCAGAGT238200.020.171489
TATGCCG67800.019.72814442-43
TACATGG157900.019.4723362
CGTATGC68750.019.46896440-41
CTCGTAT67750.019.43104238-39
Produced by FastQC (version 0.11.5)