##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140045_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6916870
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.48695233537713	18.0	18.0	30.0	18.0	33.0
2	30.979460507426047	32.0	29.0	33.0	27.0	33.0
3	31.81015415932351	33.0	31.0	33.0	29.0	33.0
4	32.616396289072945	33.0	33.0	33.0	31.0	34.0
5	33.00802212561462	33.0	33.0	34.0	32.0	34.0
6	36.985615169867295	38.0	37.0	38.0	35.0	38.0
7	37.20731327898312	38.0	38.0	38.0	36.0	38.0
8	37.13536975539514	38.0	38.0	38.0	36.0	38.0
9	37.374027558707915	38.0	38.0	38.0	37.0	38.0
10-11	37.44120570431423	38.0	38.0	38.0	37.0	38.0
12-13	37.48059577525673	38.0	38.0	38.0	37.0	38.0
14-15	37.45477795592515	38.0	38.0	38.0	37.0	38.0
16-17	37.460876740491	38.0	38.0	38.0	37.0	38.0
18-19	37.41672830340892	38.0	38.0	38.0	37.0	38.0
20-21	37.50168758143466	38.0	38.0	38.0	37.0	38.0
22-23	37.525136446037855	38.0	38.0	38.0	37.5	38.0
24-25	37.53414132902611	38.0	38.0	38.0	38.0	38.0
26-27	37.51639259705801	38.0	38.0	38.0	37.5	38.0
28-29	37.45208601026221	38.0	38.0	38.0	37.0	38.0
30-31	37.489713187442405	38.0	38.0	38.0	37.0	38.0
32-33	37.48251297533622	38.0	38.0	38.0	37.0	38.0
34-35	37.461078260150984	38.0	38.0	38.0	37.0	38.0
36-37	37.441758776908	38.0	38.0	38.0	37.0	38.0
38-39	37.41704715851776	38.0	38.0	38.0	37.0	38.0
40-41	37.41548392159635	38.0	38.0	38.0	37.0	38.0
42-43	37.35509189755177	38.0	38.0	38.0	37.0	38.0
44-45	37.36663280667601	38.0	38.0	38.0	37.0	38.0
46-47	37.351529883010755	38.0	38.0	38.0	37.0	38.0
48-49	37.32103980391143	38.0	38.0	38.0	37.0	38.0
50-51	37.289579707408805	38.0	38.0	38.0	37.0	38.0
52-53	37.26803489920889	38.0	38.0	38.0	37.0	38.0
54-55	37.25431007731538	38.0	38.0	38.0	37.0	38.0
56-57	37.255562195749874	38.0	38.0	38.0	37.0	38.0
58-59	37.24881258580447	38.0	38.0	38.0	37.0	38.0
60-61	37.24166021261884	38.0	38.0	38.0	37.0	38.0
62-63	37.241530648964506	38.0	38.0	38.0	37.0	38.0
64-65	37.23847163833578	38.0	38.0	38.0	37.0	38.0
66-67	37.23604417789963	38.0	38.0	38.0	37.0	38.0
68-69	37.23566122610934	38.0	38.0	38.0	37.0	38.0
70-71	37.23783727352397	38.0	38.0	38.0	37.0	38.0
72-73	37.20857770707981	38.0	38.0	38.0	37.0	38.0
74-75	37.20404929762608	38.0	38.0	38.0	37.0	38.0
76-77	37.198964347366925	38.0	38.0	38.0	37.0	38.0
78-79	37.184534750698674	38.0	38.0	38.0	36.0	38.0
80-81	37.17609364272677	38.0	38.0	38.0	36.0	38.0
82-83	37.17475280833369	38.0	38.0	38.0	36.0	38.0
84-85	37.15620923766012	38.0	38.0	38.0	36.0	38.0
86-87	37.13671417898435	38.0	38.0	38.0	36.0	38.0
88-89	37.11947566874427	38.0	38.0	38.0	36.0	38.0
90-91	37.10835730216626	38.0	38.0	38.0	36.0	38.0
92-93	37.10064542959644	38.0	38.0	38.0	36.0	38.0
94-95	37.06009945763799	38.0	38.0	38.0	36.0	38.0
96-97	37.07139685126179	38.0	38.0	38.0	36.0	38.0
98-99	37.060499168322934	38.0	38.0	38.0	36.0	38.0
100	35.44012922805392	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	9.0
19	22.0
20	72.0
21	181.0
22	506.0
23	1139.0
24	2625.0
25	5018.0
26	8465.0
27	13835.0
28	21642.0
29	32205.0
30	44063.0
31	59144.0
32	77391.0
33	105298.0
34	157224.0
35	288772.0
36	983714.0
37	5115543.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.8302281234142	18.866510430295786	20.340630950126286	23.96263049616373
2	13.908430927061504	21.086216206643048	42.41686431188741	22.588488554408038
3	17.631168433120763	25.994055114524343	31.167522304163587	25.207254148191303
4	12.034388386654657	18.481928964979826	39.385299998409685	30.098382649955834
5	12.99402884988382	37.57325137574726	36.140155106187116	13.292564668181805
6	29.237127388512928	38.40639446592498	19.102089157270296	13.25438898829179
7	25.805169101052932	32.732666654136914	23.512672639503126	17.949491605307024
8	23.601907163486832	37.15017589605933	21.777931604603182	17.46998533585066
9	24.911404886032102	17.121106976286928	21.703113840673367	36.2643742970076
10-11	23.552264246689617	27.027745208454114	29.56290923495743	19.85708130989884
12-13	24.43512022056219	24.48177427073228	29.466622908916897	21.616482599788633
14-15	21.627882264665953	25.99882605860743	27.01699612686085	25.356295549865767
16-17	21.333211409206765	29.17719286324595	27.74719634748087	21.74239938006642
18-19	21.3580420045483	28.23503983738309	29.4003646157872	21.00655354228141
20-21	22.03389279161926	27.400752659101524	29.303493951691607	21.261860597587617
22-23	21.69898395576573	27.560322746864514	29.125088120974556	21.615605176395203
24-25	21.584562196040512	27.633103468816984	29.334925991356243	21.447408343786268
26-27	21.65495131811408	27.80585634397258	29.22667131694573	21.312521020967612
28-29	21.467563453626827	27.64273920184765	29.415900043217487	21.473797301308036
30-31	21.91068096643588	27.599695727005457	29.19428453143841	21.295338775120253
32-33	21.674829555317498	27.516804461486455	29.225311823893335	21.583054159302716
34-35	21.51455257247685	27.758133164336922	29.467477409461768	21.25983685372446
36-37	21.707081216113572	27.62029446912817	29.20366763577165	21.468956678986608
38-39	21.41924467989089	27.568702274125172	29.40660647194555	21.605446574038385
40-41	21.69818123583238	27.476174212998437	29.337194240403182	21.488450310766005
42-43	21.76486832734302	27.47475956440245	29.18300691399728	21.57736519425725
44-45	21.638824506098782	27.704573074966603	29.08219761992029	21.57440479901432
46-47	21.849414397181715	27.510051009796516	28.746512584794342	21.894022008227427
48-49	21.72937430875493	27.544875715226556	28.87945216802132	21.846297807997193
50-51	21.612959353801305	27.723569063585668	28.86285845399809	21.80061312861494
52-53	21.764257860313243	27.69047108696349	28.933448359587636	21.611822693135625
54-55	21.70117320847863	27.651772005075863	28.764235584860593	21.882819201584912
56-57	21.894024806226	27.872083437122598	28.48584194748444	21.748049809166965
58-59	21.612876831741744	28.124137529522645	28.797065122126607	21.465920516609
60-61	21.836848661747858	28.33551685988293	28.420373488099816	21.407260990269396
62-63	21.63188391155574	28.553120936758987	28.355311933823778	21.459683217861492
64-65	21.613780414255675	28.457247830390937	28.373382282167963	21.555589473185428
66-67	21.6584154389061	28.42448727534438	28.301011686419507	21.61608559933001
68-69	21.712163101314022	28.311368332976	28.326612199086696	21.64985636662328
70-71	21.70673225420118	28.033755593771776	28.43745038921426	21.822061762812783
72-73	21.845432812123462	27.80061378251288	28.48690947968303	21.867043925680623
74-75	21.761529181983345	27.86611399441698	28.422432063129982	21.949924760469692
76-77	21.84257771380907	27.863826280352633	28.33230332952048	21.961292676317814
78-79	21.862731929085925	27.87735891839923	28.326085200722666	21.93382395179218
80-81	21.813407221699286	27.886389310841203	28.317423192080675	21.982780275378833
82-83	21.935051018310023	27.857451126587385	28.34896131312191	21.85853654198068
84-85	22.045779612780915	27.835666064628484	28.249555093515916	21.868999229074685
86-87	21.87842793026086	27.824470944971925	28.313942254752135	21.98315887001508
88-89	21.938762698999835	27.802201318608322	28.306920586079166	21.95211539631268
90-91	21.973525887873606	27.870551426916744	28.260497818676118	21.895424866533535
92-93	21.99332301477682	27.873484317413606	28.241361679287238	21.89183098852234
94-95	21.973423373871032	27.949048370597033	28.176656661330984	21.900871594200947
96-97	22.004387306884546	28.076332262360303	28.25529745030653	21.663982980448623
98-99	23.105482345580544	29.91370755061239	28.54211889945214	18.438691204354928
100	21.88261857164692	30.87289252621432	22.53286639783069	24.71162250430807
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	878.0
1	1084.0
2	1741.0
3	2414.5
4	3019.5
5	3635.0
6	4156.0
7	5855.0
8	7748.5
9	8510.5
10	8996.5
11	9567.0
12	10689.5
13	12699.5
14	16383.0
15	20398.0
16	22605.5
17	22968.5
18	21845.5
19	20188.0
20	19160.5
21	19332.5
22	21645.0
23	26647.5
24	34246.5
25	44673.5
26	58434.0
27	74246.5
28	89740.0
29	107048.5
30	124881.0
31	141491.0
32	159522.5
33	176941.5
34	194039.5
35	210497.5
36	227009.5
37	242683.5
38	252988.0
39	261661.0
40	272406.5
41	280855.5
42	288463.0
43	307356.0
44	314465.5
45	308434.5
46	303781.5
47	294247.5
48	286719.5
49	278244.5
50	267961.0
51	255468.0
52	240114.5
53	223831.0
54	206765.5
55	188243.5
56	169503.5
57	150908.5
58	132743.5
59	113938.0
60	94260.5
61	75681.0
62	58941.0
63	45385.0
64	34434.5
65	25491.0
66	18642.5
67	14007.0
68	10635.0
69	7792.5
70	5653.0
71	4076.0
72	2942.0
73	2037.5
74	1344.0
75	872.5
76	524.0
77	295.5
78	195.0
79	129.0
80	88.5
81	59.0
82	33.5
83	18.5
84	10.0
85	6.0
86	5.0
87	4.0
88	2.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.1686109468589118E-4
3	0.0
4	0.0
5	0.006462460621639556
6	2.024036883734984E-4
7	0.0
8	1.4457406312392744E-5
9	1.4457406312392743E-4
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	7.22927224868176E-5
26-27	2.1689729717771067E-5
28-29	6.147027792737555E-4
30-31	2.2436826040267156E-4
32-33	0.0015371798116084628
34-35	2.980057455507742E-4
36-37	0.001400498416962662
38-39	6.745337340552506E-4
40-41	7.963002122582454E-4
42-43	1.3348039907079844E-4
44-45	9.706251497935929E-5
46-47	4.740664934693578E-4
48-49	0.0016606667812198153
50-51	0.001825481661738248
52-53	0.0017919655647283342
54-55	0.0013865322788974821
56-57	0.003106751008972031
58-59	4.7204546741942184E-4
60-61	1.585808535979181E-5
62-63	0.0020780343457516668
64-65	0.0024658619146337947
66-67	0.0033755362120611084
68-69	8.710704757647238E-4
70-71	0.005072912531779389
72-73	0.0010586980870333851
74-75	0.0040780638065224094
76-77	2.823483028129078E-4
78-79	0.0010624818816605953
80-81	0.0
82-83	0.0
84-85	9.866466706274753E-4
86-87	0.0
88-89	1.8129931964710813E-5
90-91	0.00308657579494439
92-93	5.18238717596083E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	32.0
20-21	151.0
22-23	285.0
24-25	483.0
26-27	1325.0
28-29	3587.0
30-31	11748.0
32-33	15480.0
34-35	21371.0
36-37	33150.0
38-39	38468.0
40-41	38130.0
42-43	43514.0
44-45	51279.0
46-47	51813.0
48-49	48417.0
50-51	45310.0
52-53	45389.0
54-55	49033.0
56-57	50260.0
58-59	49297.0
60-61	50182.0
62-63	50601.0
64-65	54481.0
66-67	61324.0
68-69	63874.0
70-71	72620.0
72-73	54971.0
74-75	52853.0
76-77	55092.0
78-79	55879.0
80-81	55161.0
82-83	53004.0
84-85	53113.0
86-87	55491.0
88-89	56274.0
90-91	56625.0
92-93	56256.0
94-95	66607.0
96-97	208341.0
98-99	459978.0
100-101	4625621.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.15837674375005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.62528694697146	78.98752902234591
2	6.833719853258664	11.912318590631259
3	1.5159647353033883	3.963870765894361
4	0.5129763106815333	1.7884073018800022
5	0.21090213829886806	0.919094401295761
6	0.1105135642924276	0.5779309723136429
7	0.06120524820088006	0.3734185056970924
8	0.036634308999957545	0.2554389524452202
9	0.021749366832095024	0.17060755574607836
>10	0.06939294032077076	0.8876824826163076
>50	0.0012568204270875748	0.07668540028083175
>100	3.5007873874447204E-4	0.05596788866567917
>500	4.768767414010598E-5	0.0310481601878791
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.8914812624785487E-5	0.0	1.4457406312392744E-5	0.0
3	0.0	2.8914812624785487E-5	0.0	1.4457406312392744E-5	0.0
4	0.0	8.674443787435647E-5	0.0	1.4457406312392744E-5	0.0
5	0.0	1.0120184418674921E-4	0.0	1.4457406312392744E-5	0.0
6	1.4457406312392744E-5	1.0120184418674921E-4	0.0	1.4457406312392744E-5	0.0
7	1.4457406312392744E-5	1.0120184418674921E-4	0.0	1.4457406312392744E-5	0.0
8	1.4457406312392744E-5	1.0120184418674921E-4	0.0	1.4457406312392744E-5	0.0
9	1.4457406312392744E-5	1.1565925049914195E-4	0.0	1.4457406312392744E-5	0.0
10-11	2.8914812624785487E-5	1.1565925049914195E-4	0.0	7.228703156196372E-5	0.0
12-13	2.8914812624785487E-5	1.1565925049914195E-4	0.0	8.674443787435647E-5	0.0
14-15	2.8914812624785487E-5	1.6626017259251656E-4	0.0	1.0843054734294559E-4	0.0
16-17	8.674443787435647E-5	1.7348887574871293E-4	0.0	1.1565925049914195E-4	0.0
18-19	8.674443787435647E-5	1.807175789049093E-4	0.0	1.3011665681153468E-4	0.0
20-21	8.674443787435647E-5	1.8794628206110568E-4	0.0	1.3734535996773106E-4	0.0
22-23	8.674443787435647E-5	1.8794628206110568E-4	0.0	1.7348887574871293E-4	0.0
24-25	8.674443787435647E-5	2.096323915296948E-4	0.0	2.096323915296948E-4	0.0
26-27	8.674443787435647E-5	2.1686109468589115E-4	0.0	2.457759073106767E-4	0.0
28-29	8.674443787435647E-5	2.2408979784208752E-4	0.0	4.264934862155859E-4	0.0
30-31	8.674443787435647E-5	2.746907199354621E-4	0.0	0.0010409332544922775	0.0
32-33	8.674443787435647E-5	2.8914812624785487E-4	0.0	0.0025155886983563376	0.0
34-35	8.674443787435647E-5	3.975786735908005E-4	0.0	0.004236020049531074	0.0
36-37	8.674443787435647E-5	5.204666272461387E-4	0.0	0.007264846671977354	0.0
38-39	8.674443787435647E-5	5.566101430271207E-4	0.0	0.011464723205727447	0.0
40-41	1.3011665681153468E-4	6.072110651204952E-4	0.0	0.01595374786572539	0.0
42-43	1.3011665681153468E-4	6.433545809014772E-4	0.0	0.02014639569631929	0.0
44-45	1.3734535996773106E-4	6.867267998386553E-4	0.0	0.024808909232065947	0.0
46-47	1.4457406312392743E-4	7.228703156196372E-4	0.0	0.030309952333931387	0.0
48-49	1.4457406312392743E-4	7.300990187758336E-4	0.0	0.03618688799991904	0.0
50-51	1.5903146943632018E-4	8.602156755873683E-4	0.0	0.04136263945975564	0.0
52-53	1.5903146943632018E-4	8.746730818997609E-4	0.0	0.046993799218432615	0.0
54-55	1.5903146943632018E-4	8.819017850559573E-4	0.0	0.05281290525917069	0.0
56-57	1.5903146943632018E-4	8.819017850559573E-4	0.0	0.058574181674659206	0.0
58-59	1.5903146943632018E-4	8.963591913683501E-4	0.0	0.0643499154964601	0.0
60-61	1.5903146943632018E-4	9.397314103055283E-4	0.0	0.06940277900264137	0.0
62-63	1.5903146943632018E-4	9.686462229303138E-4	0.0	0.07436889807094828	0.0
64-65	1.5903146943632018E-4	0.0010264758481798848	0.0	0.07946513379606672	0.0
66-67	1.5903146943632018E-4	0.0010264758481798848	0.0	0.08499509171055694	0.0
68-69	1.5903146943632018E-4	0.0010626193639608665	0.0	0.09013469965461257	0.0
70-71	1.5903146943632018E-4	0.001069848067117063	0.0	0.09489841503454596	0.0
72-73	1.7348887574871293E-4	0.001069848067117063	0.0	0.09855613883158135	0.0
74-75	1.7348887574871293E-4	0.0010915341765856523	0.0	0.10120184418674921	0.0
76-77	1.7348887574871293E-4	0.001163821208147616	0.0	0.10432464395022605	0.0
78-79	1.7348887574871293E-4	0.001185507317616205	0.0	0.10628362250555526	0.0
80-81	1.7348887574871293E-4	0.001185507317616205	0.0	0.1069848067117063	0.0
82-83	1.7348887574871293E-4	0.001185507317616205	0.0	0.10741852890107809	0.0
84-85	1.7348887574871293E-4	0.001185507317616205	0.0	0.10761370388629539	0.0
86-87	1.7348887574871293E-4	0.001185507317616205	0.0	0.10774382054310692	0.0
88	1.7348887574871293E-4	0.0012288795365533833	0.0	0.10775104924626312	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6955	0.0	45.751804	1
CTTATAC	6175	0.0	36.857513	1
GTATCAA	12555	0.0	33.553623	1
ATCAACG	15820	0.0	30.509895	3
TCAACGC	15905	0.0	30.400839	4
CAACGCA	16400	0.0	29.430882	5
TATCAAC	16840	0.0	28.942823	2
AACGCAG	17000	0.0	28.364836	6
TATACAC	9555	0.0	27.324596	3
TTATACA	9440	0.0	25.88376	2
ACGCAGA	19765	0.0	24.353323	7
CGCAGAG	19890	0.0	24.200274	8
ACACATC	10380	0.0	22.58628	6
ACATCTC	10570	0.0	22.424023	8
TACACAT	12740	0.0	20.661978	5
GCAGAGT	23820	0.0	20.17148	9
TATGCCG	6780	0.0	19.728144	42-43
TACATGG	15790	0.0	19.472336	2
CGTATGC	6875	0.0	19.468964	40-41
CTCGTAT	6775	0.0	19.431042	38-39
>>END_MODULE