FastQCFastQC Report
Fri 27 May 2016
SRR2140044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7007224
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83050.050.4059521
GTATCAA158800.034.990411
ATCAACG199300.030.4028223
TCAACGC201450.030.036744
CAACGCA204800.029.5249565
TATCAAC210100.029.059592
AACGCAG211100.028.6217276
ACGCAGA235700.025.6522547
CGCAGAG240050.025.1874038
CTTATAC47950.024.9064711
TGGTATC38000.021.8342132
GTGGTAT36700.021.5800151
GCAGAGT282150.021.4143059
TATACAC70500.021.2193453
GAGTACT184800.020.44007712-13
CAGAGTA276550.019.38587610-11
AGAGTAC259450.018.6690810-11
TACATGG171100.018.1478082
GTACTTT208200.018.11278514-15
TATGCCG37000.018.00140442-43
Produced by FastQC (version 0.11.5)