##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140044_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7007224
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.693115561882994	18.0	18.0	30.0	18.0	33.0
2	31.119374377071434	32.0	31.0	33.0	27.0	33.0
3	31.88102934914026	33.0	31.0	33.0	29.0	33.0
4	32.64908528684112	33.0	33.0	33.0	31.0	34.0
5	33.03085016263216	33.0	33.0	34.0	33.0	34.0
6	37.05601604858072	38.0	38.0	38.0	36.0	38.0
7	37.29232403588068	38.0	38.0	38.0	36.0	38.0
8	37.23260295375173	38.0	38.0	38.0	36.0	38.0
9	37.452048771382216	38.0	38.0	38.0	37.0	38.0
10-11	37.50939252120383	38.0	38.0	38.0	37.0	38.0
12-13	37.54278620178262	38.0	38.0	38.0	37.5	38.0
14-15	37.512761187597256	38.0	38.0	38.0	37.5	38.0
16-17	37.52028535123181	38.0	38.0	38.0	37.0	38.0
18-19	37.47510897325389	38.0	38.0	38.0	37.0	38.0
20-21	37.55753820496018	38.0	38.0	38.0	38.0	38.0
22-23	37.57628534650969	38.0	38.0	38.0	38.0	38.0
24-25	37.58611657853842	38.0	38.0	38.0	38.0	38.0
26-27	37.56716969173638	38.0	38.0	38.0	38.0	38.0
28-29	37.504046500649295	38.0	38.0	38.0	38.0	38.0
30-31	37.539768385960905	38.0	38.0	38.0	38.0	38.0
32-33	37.53010660380559	38.0	38.0	38.0	38.0	38.0
34-35	37.50881921748413	38.0	38.0	38.0	38.0	38.0
36-37	37.489814165146655	38.0	38.0	38.0	38.0	38.0
38-39	37.46841542151447	38.0	38.0	38.0	38.0	38.0
40-41	37.44960457367793	38.0	38.0	38.0	38.0	38.0
42-43	37.38307173554594	38.0	38.0	38.0	37.0	38.0
44-45	37.3897025946496	38.0	38.0	38.0	37.0	38.0
46-47	37.36664150250222	38.0	38.0	38.0	37.0	38.0
48-49	37.329854359732664	38.0	38.0	38.0	37.0	38.0
50-51	37.29671007982625	38.0	38.0	38.0	37.0	38.0
52-53	37.276548528851436	38.0	38.0	38.0	37.0	38.0
54-55	37.26577702299572	38.0	38.0	38.0	37.0	38.0
56-57	37.26916697413222	38.0	38.0	38.0	37.0	38.0
58-59	37.265939506475675	38.0	38.0	38.0	37.0	38.0
60-61	37.264160514333	38.0	38.0	38.0	37.0	38.0
62-63	37.2682952377754	38.0	38.0	38.0	37.0	38.0
64-65	37.27131801785734	38.0	38.0	38.0	37.0	38.0
66-67	37.27496551991871	38.0	38.0	38.0	37.0	38.0
68-69	37.27956446003138	38.0	38.0	38.0	37.0	38.0
70-71	37.288474937604484	38.0	38.0	38.0	37.0	38.0
72-73	37.26377519518481	38.0	38.0	38.0	37.0	38.0
74-75	37.262016085161534	38.0	38.0	38.0	37.0	38.0
76-77	37.260456816394054	38.0	38.0	38.0	37.0	38.0
78-79	37.24736794441749	38.0	38.0	38.0	37.0	38.0
80-81	37.23981108824172	38.0	38.0	38.0	37.0	38.0
82-83	37.24252455560568	38.0	38.0	38.0	37.0	38.0
84-85	37.225618815563614	38.0	38.0	38.0	37.0	38.0
86-87	37.21251163646634	38.0	38.0	38.0	36.5	38.0
88-89	37.1963153625477	38.0	38.0	38.0	36.0	38.0
90-91	37.188130430353894	38.0	38.0	38.0	36.0	38.0
92-93	37.184311517678836	38.0	38.0	38.0	36.0	38.0
94-95	37.14775728471159	38.0	38.0	38.0	36.0	38.0
96-97	37.161042330131956	38.0	38.0	38.0	36.0	38.0
98-99	37.14960401693583	38.0	38.0	38.0	36.0	38.0
100	35.60977643914597	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	24.0
20	57.0
21	148.0
22	457.0
23	1121.0
24	2441.0
25	4438.0
26	7982.0
27	12777.0
28	20532.0
29	30767.0
30	43084.0
31	57054.0
32	74006.0
33	98141.0
34	144309.0
35	265248.0
36	979794.0
37	5264841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.93311017315844	18.993313186505812	19.343680179197925	23.72989646113782
2	14.36678185285262	21.27918031808969	41.59542333991608	22.758614489141607
3	17.627251533560223	25.76435404376969	31.492171507575613	25.116222915094475
4	12.313463933791756	18.350448051896155	39.04342147475234	30.292666539559747
5	13.071788805126932	37.462702095875684	35.95591458021759	13.50959451877979
6	29.556624235036168	38.15854106508254	18.851126428068067	13.433708271813224
7	25.75863137813205	32.98966894736061	23.439781573987073	17.811918100520263
8	23.875767030072687	36.53031115611864	21.795199295812235	17.79872251799643
9	25.18819193036656	16.654893075815647	21.818704396958104	36.33821059685969
10-11	23.658356005174088	26.88868658972512	29.508861141016755	19.94409626408404
12-13	24.50331258141598	24.6469429263286	29.390997062460112	21.45874742979531
14-15	21.759716954939428	26.11261792919835	27.177312098008578	24.950353017853647
16-17	21.237647033975225	29.079211682115485	27.98195833328576	21.70118295062353
18-19	21.233765325612538	28.082397537170213	29.851778678689307	20.832058458527943
20-21	22.01911520721675	27.286354275461893	29.71443356340569	20.980096953915673
22-23	21.759839141980194	27.286436438697176	29.488458167345744	21.46526625197688
24-25	21.57680153560682	27.328240810187026	29.690696370974244	21.40426128323191
26-27	21.541419251125536	27.53855856970782	29.60042314275708	21.31959903640956
28-29	21.44604939053468	27.496206750331524	29.64173916222285	21.416004696910946
30-31	21.714515373453963	27.364305546490524	29.612877956076705	21.308301123978808
32-33	21.43424580094908	27.354845591428433	29.626941711138787	21.583966896483698
34-35	21.53517269118784	27.40032314501915	29.538915733694253	21.52558843009875
36-37	21.55053700328923	27.383098804076933	29.645823701986156	21.42054049064768
38-39	21.403374416935655	27.444841513809777	29.716806320899416	21.43497774835516
40-41	21.70495906729252	27.270352571242228	29.544593307482693	21.480095053982556
42-43	21.713142253991535	27.35804612611568	29.366689632791488	21.5621219871013
44-45	21.724141071476026	27.518984200809264	29.197105138924844	21.559769588789862
46-47	21.80998246295123	27.410197500393423	28.92636838545235	21.85345165120299
48-49	21.71058898225718	27.493019476882548	29.019750296846546	21.776641244013728
50-51	21.658733632228508	27.651725676276673	28.930355025112625	21.7591856663822
52-53	21.842018151747716	27.600265284152176	28.861932073262054	21.695784490838054
54-55	21.8244472666899	27.56676755598198	28.746000336236815	21.8627848410913
56-57	21.87718254461032	27.708625360933066	28.59260585722008	21.821586237236538
58-59	21.7063210007405	27.83804635074059	28.839563381970635	21.616069266548273
60-61	21.888144644948532	27.920465089678316	28.601561629698214	21.58982863567494
62-63	21.788889735861744	27.992395812566212	28.54649522633069	21.672219225241353
64-65	21.74708372676841	27.983290876073998	28.54871034737952	21.72091504977807
66-67	21.826289720667972	27.987475064097122	28.420914712972994	21.765320502261908
68-69	21.786834106468895	27.888820999344382	28.51737680074819	21.806968093438535
70-71	21.768804062205803	27.677013350084884	28.593125175548074	21.96105741216124
72-73	21.907679641343332	27.55340753839855	28.590645277782663	21.94826754247545
74-75	21.836027930060645	27.643402222775315	28.47837486416591	22.042194982998126
76-77	21.922540536651226	27.634432627965737	28.383459508495307	22.059567326887723
78-79	21.89169290243328	27.688543753906814	28.355800357281087	22.063962986378815
80-81	21.91663593369623	27.673802283102344	28.333987114271125	22.075574668930297
82-83	21.983584663601782	27.659518500271542	28.391606845916108	21.965289990210568
84-85	22.10528688844938	27.622493986565612	28.258217066826223	22.014002058158784
86-87	21.925391943441436	27.648700266422615	28.33611250376109	22.08979528637486
88-89	22.021105257796414	27.67323356316146	28.215899077089933	22.089762101952196
90-91	22.06413876095144	27.711316993539565	28.21571818347689	22.008826062032103
92-93	22.10539232961369	27.68143243506155	28.202128335025495	22.01104690029927
94-95	22.039713289765665	27.741638585301093	28.17323037236963	22.045417752563615
96-97	22.084938884689503	27.902212700280366	28.193426756876853	21.819421658153278
98-99	23.17431624398533	29.743277002411116	28.449867890313175	18.632538863290378
100	29.27467153057886	40.8739202559646	29.851408213456537	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2046.0
1	2051.0
2	2554.5
3	3275.0
4	3999.0
5	4879.0
6	5643.0
7	8304.5
8	11016.0
9	11709.5
10	11846.0
11	12229.5
12	13489.0
13	15994.0
14	20804.0
15	25839.0
16	28540.5
17	29041.0
18	27774.0
19	25842.5
20	24541.5
21	24359.0
22	26369.5
23	31772.5
24	40802.5
25	52334.0
26	67225.0
27	83967.5
28	100382.5
29	117871.0
30	134828.5
31	151223.0
32	168711.5
33	185849.0
34	202124.0
35	217555.5
36	233579.5
37	248004.0
38	258189.5
39	267224.0
40	276895.5
41	285275.0
42	290611.0
43	295083.0
44	304810.0
45	313459.0
46	309407.5
47	303274.5
48	296640.0
49	286209.0
50	276769.5
51	264195.5
52	248564.0
53	233396.5
54	218324.0
55	201151.0
56	181941.5
57	162956.5
58	145145.0
59	125200.0
60	103673.0
61	83889.0
62	66441.5
63	51271.5
64	38753.5
65	29121.0
66	21572.5
67	16545.5
68	12595.5
69	9130.5
70	6748.0
71	4926.0
72	3483.5
73	2375.0
74	1636.5
75	1096.5
76	698.0
77	438.0
78	266.5
79	173.0
80	120.5
81	84.5
82	54.0
83	28.0
84	16.0
85	12.0
86	8.5
87	4.5
88	2.0
89	2.0
90	2.5
91	3.0
92	3.0
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.5687775929526444E-4
3	0.0
4	0.0
5	0.006607466808539302
6	4.2812959882544075E-4
7	0.0
8	2.854197325502938E-5
9	2.2833578604023505E-4
10-11	0.0
12-13	0.0
14-15	7.135493313757345E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	7.849868832259935E-5
26-27	1.4274143053606044E-5
28-29	6.42586751531909E-4
30-31	2.7880104114317005E-4
32-33	0.0013906678445766677
34-35	2.230566299050153E-4
36-37	0.0012152439330935878
38-39	7.212326521691618E-4
40-41	6.458826048181227E-4
42-43	9.614405396728455E-5
44-45	5.225486069115414E-5
46-47	5.05453122504561E-4
48-49	0.0014325850616430684
50-51	0.0015765076065338472
52-53	0.0016135867729947944
54-55	0.0014172799469295218
56-57	0.0029106338901871615
58-59	3.834705345635175E-4
60-61	2.4199551437114558E-5
62-63	0.0018730779573417084
64-65	0.0024679061149284133
66-67	0.003052943107864473
68-69	6.817900608476587E-4
70-71	0.004666869806084747
72-73	0.001077848514341033
74-75	0.003992589126924496
76-77	3.2625319805288565E-4
78-79	0.0010617320454196478
80-81	1.805608708161902E-5
82-83	3.653453161725767E-5
84-85	8.684918546551163E-4
86-87	0.0
88-89	9.467296974053074E-6
90-91	0.0027804189094180745
92-93	5.533710344048247E-4
94-95	9.835279718797551E-6
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	42.0
20-21	182.0
22-23	411.0
24-25	608.0
26-27	2002.0
28-29	5516.0
30-31	17977.0
32-33	24067.0
34-35	33047.0
36-37	47062.0
38-39	51543.0
40-41	50370.0
42-43	59409.0
44-45	69606.0
46-47	68169.0
48-49	61233.0
50-51	56652.0
52-53	58122.0
54-55	63456.0
56-57	63810.0
58-59	60509.0
60-61	59044.0
62-63	60409.0
64-65	65715.0
66-67	69954.0
68-69	69840.0
70-71	73629.0
72-73	63347.0
74-75	64588.0
76-77	66250.0
78-79	65766.0
80-81	64987.0
82-83	62481.0
84-85	63572.0
86-87	66038.0
88-89	66135.0
90-91	65265.0
92-93	65074.0
94-95	75101.0
96-97	208575.0
98-99	1534707.0
100-101	3282954.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.85885875947132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.60822178802053	78.70126738732527
2	6.690354792112741	11.622331638777453
3	1.5777423647592652	4.111227036583779
4	0.5634731018890748	1.9577052228697736
5	0.23656173437218556	1.027374113686462
6	0.11505173073425182	0.5995957217927462
7	0.07077128104924865	0.4302978893418477
8	0.040427911219824274	0.280921778446653
9	0.02871277565791822	0.2244563032917154
>10	0.066961375410698	0.853808870011643
>50	0.0011204768452648564	0.06495588326757414
>100	5.291330844259726E-4	0.083703248146807
>500	7.153484454904162E-5	0.04235490645832936
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.427098662751469E-5	0.0	0.0	0.0
3	0.0	1.427098662751469E-5	0.0	0.0	0.0
4	0.0	7.135493313757346E-5	0.0	0.0	0.0
5	0.0	8.562591976508814E-5	0.0	1.427098662751469E-5	0.0
6	1.4270986627514692E-4	8.562591976508814E-5	0.0	1.427098662751469E-5	1.427098662751469E-5
7	1.569808529026616E-4	1.1416789302011752E-4	0.0	1.427098662751469E-5	1.427098662751469E-5
8	1.7125183953017628E-4	1.2843887964763222E-4	0.0	1.427098662751469E-5	1.427098662751469E-5
9	1.7125183953017628E-4	2.4260677266774974E-4	0.0	2.854197325502938E-5	1.427098662751469E-5
10-11	1.7125183953017628E-4	2.4260677266774974E-4	0.0	4.994845319630141E-5	1.427098662751469E-5
12-13	1.7125183953017628E-4	2.4260677266774974E-4	0.0	5.708394651005876E-5	1.427098662751469E-5
14-15	1.7125183953017628E-4	3.5677466568786727E-4	0.0	5.708394651005876E-5	1.427098662751469E-5
16-17	1.8552282615769098E-4	3.7104565231538196E-4	0.0	5.708394651005876E-5	1.427098662751469E-5
18-19	1.8552282615769098E-4	3.92452132256654E-4	0.0	7.135493313757346E-5	2.854197325502938E-5
20-21	1.9265831947144833E-4	4.13858612197926E-4	0.0	8.562591976508816E-5	2.854197325502938E-5
22-23	2.0692930609896303E-4	4.281295988254407E-4	0.0	1.3557437296138954E-4	2.854197325502938E-5
24-25	2.1406479941272035E-4	4.566715720804701E-4	0.0	1.7125183953017628E-4	4.281295988254407E-5
26-27	2.1406479941272035E-4	4.780780520217421E-4	0.0	2.2120029272647772E-4	4.281295988254407E-5
28-29	2.1406479941272035E-4	5.066200252767715E-4	0.0	4.6380706539422747E-4	4.281295988254407E-5
30-31	2.1406479941272035E-4	5.422974918455583E-4	0.0	0.0013771502095551677	4.281295988254407E-5
32-33	2.354712793539924E-4	5.637039717868303E-4	0.0	0.003232378471132077	7.135493313757346E-5
34-35	2.5687775929526444E-4	6.564653848656757E-4	0.0	0.005765478597515935	8.562591976508814E-5
36-37	2.5687775929526444E-4	7.135493313757345E-4	0.0	0.00949020610729727	8.562591976508814E-5
38-39	2.5687775929526444E-4	7.492267979445212E-4	0.0	0.014877503559184064	8.562591976508814E-5
40-41	2.8541973255029383E-4	8.990721575334254E-4	0.0	0.020500272290424854	9.989690639260284E-5
42-43	2.8541973255029383E-4	9.632915973572416E-4	0.0	0.02615871848823443	9.989690639260284E-5
44-45	2.996907191778085E-4	0.0011060014636323885	0.0	0.032616339937184824	9.989690639260284E-5
46-47	3.139617058053232E-4	0.0012130338633387487	0.0	0.03996589805035489	1.2130338633387487E-4
48-49	3.139617058053232E-4	0.0012344403432800206	0.0	0.046994358964405875	1.4270986627514692E-4
50-51	3.5677466568786727E-4	0.0014770471159477705	0.0	0.053202238147374764	1.4270986627514692E-4
52-53	3.781811456291393E-4	0.0014984535958890426	0.0	0.05943152381028492	1.4984535958890424E-4
54-55	3.8531663894289666E-4	0.0015127245825165571	0.0	0.06573929989964641	1.569808529026616E-4
56-57	3.8531663894289666E-4	0.0015412665557715866	0.0	0.07271067686718735	1.569808529026616E-4
58-59	3.8531663894289666E-4	0.0015626730357128586	0.0	0.07923965324927532	1.569808529026616E-4
60-61	3.8531663894289666E-4	0.0016054859955954028	0.0	0.08519065467294895	1.569808529026616E-4
62-63	4.281295988254407E-4	0.0016411634621641895	0.0	0.09119160454981887	1.569808529026616E-4
64-65	4.281295988254407E-4	0.0018124153016943656	0.0	0.09707838653366868	1.569808529026616E-4
66-67	4.495360787667127E-4	0.0018266862883218804	0.0	0.103450382062854	1.569808529026616E-4
68-69	4.6380706539422747E-4	0.0018837702348319392	0.0	0.10967966772576415	1.569808529026616E-4
70-71	4.709425587079848E-4	0.0019123122080869684	0.0	0.1152096750439261	1.7125183953017628E-4
72-73	4.994845319630141E-4	0.001940854181341998	0.0	0.11952664849874929	1.9979381278520568E-4
74-75	4.994845319630141E-4	0.0020122091144795717	0.0	0.12285892387627398	1.9979381278520568E-4
76-77	4.994845319630141E-4	0.0020978350342446593	0.0	0.12639099306658386	1.9979381278520568E-4
78-79	4.994845319630141E-4	0.0021406479941272036	0.0	0.12853164106071108	1.9979381278520568E-4
80-81	4.994845319630141E-4	0.0021620544740684755	0.0	0.1292951388452831	2.1406479941272035E-4
82-83	4.994845319630141E-4	0.0021763254606959907	0.0	0.12990165577695248	2.4260677266774974E-4
84-85	4.994845319630141E-4	0.002212002927264777	0.0	0.13010858508305145	2.4260677266774974E-4
86-87	4.994845319630141E-4	0.002290493353716108	0.0	0.1302013464961303	2.4974226598150706E-4
88	4.994845319630141E-4	0.0023832547667949535	0.0	0.13020848198944404	2.5687775929526444E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8305	0.0	50.405952	1
GTATCAA	15880	0.0	34.99041	1
ATCAACG	19930	0.0	30.402822	3
TCAACGC	20145	0.0	30.03674	4
CAACGCA	20480	0.0	29.524956	5
TATCAAC	21010	0.0	29.05959	2
AACGCAG	21110	0.0	28.621727	6
ACGCAGA	23570	0.0	25.652254	7
CGCAGAG	24005	0.0	25.187403	8
CTTATAC	4795	0.0	24.906471	1
TGGTATC	3800	0.0	21.834213	2
GTGGTAT	3670	0.0	21.580015	1
GCAGAGT	28215	0.0	21.414305	9
TATACAC	7050	0.0	21.219345	3
GAGTACT	18480	0.0	20.440077	12-13
CAGAGTA	27655	0.0	19.385876	10-11
AGAGTAC	25945	0.0	18.66908	10-11
TACATGG	17110	0.0	18.147808	2
GTACTTT	20820	0.0	18.112785	14-15
TATGCCG	3700	0.0	18.001404	42-43
>>END_MODULE